scholarly journals Evaluation and GWAS of radicle gravitropic response in a core rice germplasms population

2021 ◽  
Author(s):  
qiaojun lou ◽  
Qingsong Li ◽  
Fangjun Feng ◽  
Ryan Joynson ◽  
Yunan Yang ◽  
...  

Abstract Aims: Gravitropism is one of the primary determinants of root development, facilitating root penetration into soil and subsequent absorption of water and nutrients. To study the gravitropism of the radicle roots, we conducted this research.Methods: The gravitropism of 226 Chinese rice micro-core accessions and drought-resistant core accessions were assessed through the modified gravity-bending experiment and genome-wide association analysis (GWAS) was used to map the associated QTLs.Results: The average value of gravitropic response speed of radicle roots was 41.05°/h from 16.77°/h to 62.83°/h. Significant difference (p < 0.002) in gravity response speed between Indica (42.49°/h) and Japonica (39.71°/h) subspecies was found. The gravitational response speed of radicle roots was significantly positively correlated with the number of deep roots (r =0.16), the growth speed of radicle roots (r =0.21) and the drought resistance coefficient (r=0.14).Conclusions: In total, 3 QTLs (quantitative traits) associated with gravitropic response speed were identified on chromosome 4,11 and 12. There are some known QTLs relating to roots traits and drought resistance located nearby the QTLs identified here, which confirms the close relationship between radicle gravitropism and the drought resistance. From within these intervals, 5 candidate genes were screened for qPCR in 6 extreme rice varieties, demonstrating that gene LOC_Os12g29350 may regulate gravitropism negatively and confirming its candidacy for further study.

2021 ◽  
Author(s):  
Qiaojun Lou ◽  
Qingsong Li ◽  
Fangjun Feng ◽  
Ryan Joynson ◽  
Yunan Yang ◽  
...  

Abstract Aims Since gravitropism is one of the primary determinants of root development, facilitating root penetration into soil and subsequent absorption of water and nutrients, we studied this response in rice. Methods The gravitropism of 226 Chinese rice micro-core accessions and drought-resistant core accessions were assessed through the modified gravity-bending experiment and genome-wide association analysis (GWAS) was used to map the associated QTLs. Results The average value of gravitropic response speed of seminal roots was 41.05°/h, ranging from 16.77°/h to 62.83°/h. The gravity response speed of Indica (42.49°/h) was significantly (P < 0.002) higher than Japonica (39.71°/h) subspecies. The gravitational response speed of seminal roots was significantly positively correlated with the number of deep roots (r = 0.16), the growth speed of seminal roots (r = 0.21) and the drought resistance coefficient (r = 0.14). Conclusions In total, 3 QTLs (quantitative traits) associated with gravitropic response speed were identified on chromosome 4, 11 and 12. There are some known QTLs relating to roots traits and drought resistance located nearby the QTLs identified here, which confirms the close relationship between radicle gravitropism and the drought resistance. From within these intervals, 5 candidate genes were screened and verified by qPCR in a few rice varieties with extreme phenotypic values, demonstrating that gene LOC_Os12g29350 may regulate gravitropism negatively. This may be a promising candidate to be confirmed in further studies.


2020 ◽  
Author(s):  
qiaojun lou ◽  
Qingsong Li ◽  
Fangjun Feng ◽  
Ryan Joynson ◽  
Yunan Yang ◽  
...  

Abstract Gravitropism is one of the primary determinants of root development, facilitating root penetration into soil and subsequent absorption of water and nutrients. In this study, the gravitropism of 226 Chinese rice micro-core accessions and drought-resistant core accessions were assessed. The average value of gravitropic response speed of radicle roots was 41.05°/h in the population ranging from 16.77°/h to 62.83°/h. We observed a highly significant difference in gravity response speed between Indica (42.49°/h) and Japonica (39.71°/h) subspecies with p-value < 0.002. The correlation analysis showed that the gravitational response speed of radicle roots was significantly positively correlated with the number of deep roots (correlation coefficient = 0.16), the growth speed of radicle roots (correlation coefficient = 0.21) and the drought resistance coefficient (correlation coefficient = 0.14). Using genome-wide association analysis (GWAS), 4 QTLs(quantitative traits) associating with gravitropic response speed were identified on chromosome 4,11 and 12. From within these intervals 5 candidate genes were screened for qPCR verification in 6 extreme rice varieties, demonstrating that gene LOC_Os12g29350 may regulate gravitropism negatively and confirming it’s candidacy for further study.


2017 ◽  
Vol 1 (1) ◽  
pp. 89
Author(s):  
Indah Purwaningsih ◽  
Supriyanto Supriyanto

Abstract: Chlorine is a green halogen-shaped halogen gas at normal temperature and serves as bleach, stain remover and disinfectant. Chlorine is now widely used for bleaching rice so that less quality rice looks like quality rice. Chlorine is very toxic and causes mucous membrane irritation, highly reactive and very powerful oxidizer. The purpose of this research was to determine the difference of chlorine level in chlorinated rice washed once, twice and 3 times. The sample in this study amounted to 11 samples calculated by replication formula. Each sample was treated 3 times, ie 1 washed once, 2 washed twice and washed 3 times. The samples then examined by iodometric titration method. Based on the results of the study using ANOVA test, 11 samples obtained the average value of chlorine after washed once amount of 0.0176%, after washed twice amount of 0.0111%, and after washed 3 times amount of 0.0052% with the value significance p = 0.03 (p <0.05) at 95% confidence level which means there was a significant difference between chlorine levels in chlorinated rice washed once, twice and 3 times.Abstrak: Klorin merupakan unsur halogen berbentuk gas berwarna kuning kehijauan pada suhu normal danberfungsi sebagai pemutih, penghilang noda maupun desinfektan. Klorin sekarang banyak digunakan untuk bahan pemutih beras agar beras yang kurang berkualitas tampak seperti beras berkualitas. Klorin sangat toksik dan menyebabkan iritasi membran mukosa, sangat reaktif dan merupakan oksidator yang sangat kuat. Tujuan dari penelitian ini ialah untuk mengetahui perbedaan kadar klorin pada beras berklorin yang dicuci sebanyak 1 kali, 2 kali dan 3 kali. Sampel dalam penelitian ini berjumlah 11 sampel yang dihitung dengan rumus replikasi. Setiap sampel diberi perlakuan sebanyak 3 kali, yaitu 1 kali pencucian, 2 kali pencucian dan 3 kali pencucian. Sampel penelitian kemudian diperiksa dengan metode titrasi iodometri. Berdasarkan hasil penelitian menggunakan uji Anova secara komputerisasi terhadap 11 sampel diperoleh nilai rata-rata kadar klorin setelah 1 kali pencucian sebesar 0,0176 %, setelah 2 kali pencucian sebesar 0,0111 %, dan setelah 3 kali pencucian sebesar 0,0052 % dengan nilai signifkansi p = 0,03 (p<0,05) pada tingkat kepercayaan 95% yang artinya ada perbedaan yang bermakna antara kadar klorin pada beras berklorin yang dicuci sebanyak 1 kali, 2 kali dan 3 kali.


2018 ◽  
Vol 5 (1) ◽  
Author(s):  
Danuri Danuri ◽  
Dwendha Lahitani

This study aims to determine the effectiveness of CSM strategies on comprehension reading skills in classes V Padokan 2 Elementary School. The research method used is a quasi-experimental method. In this study the subjects of the study were VA class students as the control class and V C as the experimental class with 28 VA and VC class students. Quantitative data analysis technique used is the calculation of independent sample t-test. Based on data analysis and discussion, researchers can conclude that CSM strategies are more effective than conventional methods of reading comprehension learning. The results of this study indicate that there is a significant difference between the experimental class and the control class as evidenced by the results of the independent sample t-test obtained by the value of Asymp. Sig (2-Tailed) 0.014 <0.05. CSM strategy is an effective learning strategy seen from the average value of the experimental class posstest which is 81.61 higher than the control class average value of 72.96.


2015 ◽  
Vol 72 (2) ◽  
pp. 123-131 ◽  
Author(s):  
Marko Igic ◽  
Nebojsa Krunic ◽  
Ljiljana Aleksov ◽  
Milena Kostic ◽  
Aleksandra Igic ◽  
...  

Background/Aim. The vertical dimension of occlusion is a very important parameter for proper reconstruction of the relationship between the jaws. The literature describes many methods for its finding, from the simple, easily applicable clinically, to quite complicated, with the use of one or more devices for determination. The aim of this study was to examine the possibility of determining the vertical dimension of occlusion using the vocals ?O? and ?E? with the control of values o btained by applying cognitive functions. Methods. This investigation was performed with the two groups of patients. The first group consisted of 50 females and 50 males, aged 18 to 30 years. In this group the distance between the reference points (on top of the nose and chin) was measured in the position of the mandible in the vertical dimension of occlusion, the vertical dimension at rest and the pronunciation of the words ?OLO? and ?ELE?. Checking the correctness of the particular value for the word ?OLO? was also performed by the phonetic method with the application of cognitive exercises when the patients counted from 89 to 80. The obtained difference in the average values i n determining the vertical dimension of occlusion and the ?OLO? and ?ELE? in the first group was used as the reference for determining the vertical dimension of occlusion in the second group of patients. The second group comprised of 31 edentulous persons (14 females and 17 males), aged from 54 to 85 years who had been made a complete denture. Results. The average value obtained for the vertical dimension of rest for the entire sample was 2.16 mm, for the word ?OLO? for the entire sample was 5.51 mm and for the word ?ELE? for the entire sample was 7.47 mm. There was no statistically significant difference between the genders for the value of the vertical dimension at rest, ?ELE? and ?OLO?. There was a statistically significant difference between the values f or the vertical dimension at rest, ?OLO? and ?ELE? for both genders. There was a statistically significant correlation between the value for the vertical dimension at rest, ?OLO? and ?ELE?, for both groups of subjects. Conclusion. Determining the vertical dimension of occlusion requires 5.5 mm subtraction from the position of the mandible in pronunciation of the word ?OLO? or 7.5 mm in pronunciation of the word ?ELE?.


2021 ◽  
Vol 14 (6) ◽  
pp. 255
Author(s):  
MinhTam Bui ◽  
Trinh Q. Long

This paper identifies whether there was a performance difference among micro, small and medium enterprises (MSMEs) led by men and by women in Vietnam during the period 2005–2013 and aims to provide explanations for the differences, if any, in various performance indicators. The paper adopts a quantitative approach using a firm-level panel dataset in the manufacturing sector in 10 provinces/cities in Vietnam in five waves from 2005 to 2013. Fixed effect models are estimated to examine the influence of firm variables and demographic, human capital characteristics of owners/managers on firms’ value added, labor productivity and employment creation. We found that men led MSMEs did not outperform those led by women on average. Although the average value added was lower for female-led firms in the informal sector, the opposite was true in the formal sector where women tend to lead medium-size firms with higher value added and labor productivity. The performance disparity was more envisaged across levels of formality and less clear from a gender perspective. Moreover, while firms owned by businessmen seemed to create more jobs, firms owned by women had a higher share of female employees. No significant difference in business constraints faced by women and by men was found.


2019 ◽  
Vol 10 (2) ◽  
pp. 1243-1248
Author(s):  
Cek Dara Manja ◽  
Dennis

The height of the maxillary alveolar ridge can be measured using panoramic radiography. The decline occurred because the height of the alveolar ridge undergoes slow physiological resorption due to the absence of mechanical stimulus. The purpose of this research is to know the difference and the average height of the maxillary alveolar ridge in edentulous and dentate women using panoramic radiography. This study is an analytical method with a cross-sectional approach. A total sample of 40 women, edentulous and dentate has been measured. Measurements were made by measuring the distance between the lowest point of the infraorbital ridge and alveolar crest maxilla on the incisor point, right and left premolar, right and left molar. A statistical test is done using independent t-test. The results showed that the average value of a dentate women sample is greater than edentulous. The average value obtained by the dentate sample is greatest in incisor point by 37,57mm± 3,34mm and the smallest at the right molar point by 33,87mm ± 2,81mm. The average value obtained by the edentulous sample is greatest in incisor point by 35,30mm ± 2,79mm and the smallest at the right molar point by 31,84mm ± 3,85mm. It can be concluded that there is a significant difference in the height of the maxillary alveolar ridge on an edentulous sample and dentate, except the right molar teeth. The average height of the maxillary alveolar ridge in an edentulous sample is 33,35mm ± 3,43mm, and the dentate sample is 35,66mm ± 3,21mm.


2017 ◽  
Vol 1 (1) ◽  
Author(s):  
Dita Yulianda

This article will describe learning to write auotobiografi text using Contextual Teaching and Learning approach. The method used in the research, namely the experiment with the design of One-Group Pretest-Postest. Technique of collecting data in the form of test, observation, and questionnaire. The population in the study of students of class XI SMK Nurul Islam Cianjur, with a sample of 22 students. From the results of the study known the average value of the initial test 60.45 and the average value of the final test 80.91. The result of calculation with t-test known value that is 11,41 and value for t.s 0,05 value t = 1,72 and for t.s 0,01 value t = 2.51. Thus, there is a significant difference between students' ability in learning to write autobiographic text before and after using Contextual Teaching and Learning approach. The difference seen in the final test results is higher than the initial test results. The use of Contextual Teaching and Learning approach also get positive response for students, this can be proved by the percentage of student answers for positive response is greater than negative response, that is 30% of students who choose strongly agree (SS)> 4% of students who choose strongly disagree (STS).Keywords: autobiographic text, contextual


2019 ◽  
pp. 10-17

The purpose of this study is to analyze real-life data in order to characterize patients with RA and cardiovascular diseases that are treated with biological agents. Material and Methods: In a retrospective study, data from real clinical practice were analyzed in 195 patients with seropositive RA, which are being treated with biological agents. Results: In patients with existing CVD, significantly higher mean ESR values ​​(23.95 vs. 19.31, p = 0.031) and CRP (6.41 vs. 3.36, p = 0.004) were detected over the period of the study period of treatment. In contrast to the laboratory parameters, mean values of clinical parameters of RA - TJC, SJC and VAS clinical sings did not show a significant difference in patients with and without CVD. The time average value of DAS28 (ESR) during the study period was significantly higher in patients with CVD (3.7 vs. 3.39, p = 0.002) compared to those without CVD. DAS28 (CRP) shows the same trend. In patients with CVD, the time average value of the follow-up indicator was 3.21, and in patients without CVD, 2.88 (p <0.001). Conclusion: The results of this study, in PA patients conducting treatment with biological agents in the real life, outside of the clinical study conditions, demonstrate that independently of treatment, patients with CVD continue to maintain higher background inflammation. An optimization of therapeutic behavior in real life is necessary to improve the long-term prognosis of these patients.


2020 ◽  
Vol 1 (2) ◽  
pp. 49
Author(s):  
Hijriyah Putri Tarmizi Hasibuan ◽  
Isra Thristy

Background: Stroke is the second largest cause of death in the world. Stroke is classified based on its etiology as ischemic stroke and hemorrhagic stroke. Most large-scale studies on the risk of total cholesterol and triglyceride levels in stroke are not distinguished between ischemic and hemorrhagic strokes. Purposes: The purpose of this study was to determine the comparison of triglyceride and total cholesterol levels in ischemic stroke patients with hemorrhagic stroke. Method: Descriptive analytic study using medical records of patients at Medan Haji General Hospital in 2018-2019. The number of ischemic stroke patients is 28 patients and hemorrhagic stroke 28 patients with a total sample of 56 patients. Results: In ischemic stroke patients, the average value of triglyceride levels was 144.75 mg/dL and the average value of total cholesterol was 250.93 mg/dL. In hemorrhagic stroke patients, the average value of triglyceride levels is 126.93 mg/dL and the average total cholesterol level is 174.25 mg/dL. Conclusion: From this study we found a significant difference in total cholesterol between ischemic and hemorrhagic strokes. No significant difference was found in triglycerides between ischemic and hemorrhagic strokes.


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