scholarly journals Evaluation and GWAS of radicle gravitropic response in a core rice germplasms population

2020 ◽  
Author(s):  
qiaojun lou ◽  
Qingsong Li ◽  
Fangjun Feng ◽  
Ryan Joynson ◽  
Yunan Yang ◽  
...  
Keyword(s):  
Correlation Coefficient ◽  
Response Speed ◽  
Resistance Coefficient ◽  
P Value ◽  
Growth Speed ◽  
Root Penetration ◽  
Deep Roots ◽  
Rice Varieties ◽  
Gravitropic Response ◽  
Significant Difference

Abstract Gravitropism is one of the primary determinants of root development, facilitating root penetration into soil and subsequent absorption of water and nutrients. In this study, the gravitropism of 226 Chinese rice micro-core accessions and drought-resistant core accessions were assessed. The average value of gravitropic response speed of radicle roots was 41.05°/h in the population ranging from 16.77°/h to 62.83°/h. We observed a highly significant difference in gravity response speed between Indica (42.49°/h) and Japonica (39.71°/h) subspecies with p-value < 0.002. The correlation analysis showed that the gravitational response speed of radicle roots was significantly positively correlated with the number of deep roots (correlation coefficient = 0.16), the growth speed of radicle roots (correlation coefficient = 0.21) and the drought resistance coefficient (correlation coefficient = 0.14). Using genome-wide association analysis (GWAS), 4 QTLs(quantitative traits) associating with gravitropic response speed were identified on chromosome 4,11 and 12. From within these intervals 5 candidate genes were screened for qPCR verification in 6 extreme rice varieties, demonstrating that gene LOC_Os12g29350 may regulate gravitropism negatively and confirming it’s candidacy for further study.

scholarly journals Evaluation and GWAS of radicle gravitropic response in a core rice germplasms population

2021 ◽  
Author(s):  
qiaojun lou ◽  
Qingsong Li ◽  
Fangjun Feng ◽  
Ryan Joynson ◽  
Yunan Yang ◽  
...  
Keyword(s):  
Drought Resistance ◽  
Response Speed ◽  
Resistance Coefficient ◽  
Growth Speed ◽  
Root Penetration ◽  
Deep Roots ◽  
Rice Varieties ◽  
Gravitropic Response ◽  
Average Value ◽  
Significant Difference

Abstract Aims: Gravitropism is one of the primary determinants of root development, facilitating root penetration into soil and subsequent absorption of water and nutrients. To study the gravitropism of the radicle roots, we conducted this research.Methods: The gravitropism of 226 Chinese rice micro-core accessions and drought-resistant core accessions were assessed through the modified gravity-bending experiment and genome-wide association analysis (GWAS) was used to map the associated QTLs.Results: The average value of gravitropic response speed of radicle roots was 41.05°/h from 16.77°/h to 62.83°/h. Significant difference (p < 0.002) in gravity response speed between Indica (42.49°/h) and Japonica (39.71°/h) subspecies was found. The gravitational response speed of radicle roots was significantly positively correlated with the number of deep roots (r =0.16), the growth speed of radicle roots (r =0.21) and the drought resistance coefficient (r=0.14).Conclusions: In total, 3 QTLs (quantitative traits) associated with gravitropic response speed were identified on chromosome 4,11 and 12. There are some known QTLs relating to roots traits and drought resistance located nearby the QTLs identified here, which confirms the close relationship between radicle gravitropism and the drought resistance. From within these intervals, 5 candidate genes were screened for qPCR in 6 extreme rice varieties, demonstrating that gene LOC_Os12g29350 may regulate gravitropism negatively and confirming its candidacy for further study.

scholarly journals Evaluation and GWAS of radicle gravitropic response in a core rice germplasm population

2021 ◽  
Author(s):  
Qiaojun Lou ◽  
Qingsong Li ◽  
Fangjun Feng ◽  
Ryan Joynson ◽  
Yunan Yang ◽  
...  
Keyword(s):  
Drought Resistance ◽  
Response Speed ◽  
Resistance Coefficient ◽  
Growth Speed ◽  
Root Penetration ◽  
Deep Roots ◽  
Rice Varieties ◽  
Gravitropic Response ◽  
Average Value ◽  
Seminal Roots

Abstract Aims Since gravitropism is one of the primary determinants of root development, facilitating root penetration into soil and subsequent absorption of water and nutrients, we studied this response in rice. Methods The gravitropism of 226 Chinese rice micro-core accessions and drought-resistant core accessions were assessed through the modified gravity-bending experiment and genome-wide association analysis (GWAS) was used to map the associated QTLs. Results The average value of gravitropic response speed of seminal roots was 41.05°/h, ranging from 16.77°/h to 62.83°/h. The gravity response speed of Indica (42.49°/h) was significantly (P < 0.002) higher than Japonica (39.71°/h) subspecies. The gravitational response speed of seminal roots was significantly positively correlated with the number of deep roots (r = 0.16), the growth speed of seminal roots (r = 0.21) and the drought resistance coefficient (r = 0.14). Conclusions In total, 3 QTLs (quantitative traits) associated with gravitropic response speed were identified on chromosome 4, 11 and 12. There are some known QTLs relating to roots traits and drought resistance located nearby the QTLs identified here, which confirms the close relationship between radicle gravitropism and the drought resistance. From within these intervals, 5 candidate genes were screened and verified by qPCR in a few rice varieties with extreme phenotypic values, demonstrating that gene LOC_Os12g29350 may regulate gravitropism negatively. This may be a promising candidate to be confirmed in further studies.

Performance of promising rice varieties under ambient and elevated temperature regimes

2019 ◽  
Vol 4 (01) ◽  
pp. 10-17
Author(s):  
Shubhechhya Regmi ◽  
Binayak P. Rajbhandari
Keyword(s):  
Elevated Temperature ◽  
Open Field ◽  
Ambient Condition ◽  
P Value ◽  
Research Station ◽  
Chamber System ◽  
Open Top Chamber ◽  
Rice Varieties ◽  
Plastic Sheet ◽  
Significant Difference

Open top chamber system was designed for undertaking research into rice crop response to increased temperature during rainy season at Regional Agricultural Research Station (RARS), Khajura, Banke in 2018. With the objective of determining the efficacy of the chamber system and to generate possible differences in climate which could alter plant response between ambient and chamber system, 5 different rice varieties (Radha-4, Sukhkhadhan-3, Sukhkhadhan-2, IR83383-G-B-141-1 and IR87751-20-4-4-2) with three temperature conditions were used: ambient condition, open top chamber with 1.2m height enclosed from base with plastic sheet and open top chamber with 1.5m height enclosed from base with plastic sheet. With 3.1°c higher temperature Sukhkhadhan-2 significantly (p value= 0.0199) produced higher grain yield 4.33 t/ha under chamber condition in contrast to open field (2.93 t/ha) as well as significantly (p value=0.0175) higher biomass yield 13.62 t/ha under elevated temperature. Also, Sukhkhadhan-2 significantly (p value=0.0012) attained maximum height (86.99 cm) under chamber condition compared to open field (73.06 cm) during harvest. Statistical analysis showed no significant difference for number of tillers per hill during maturity stage. Significantly sukhkhadhan-3 (p value=0.02994) showed longer panicle length 25.7cm under chamber than normal field condition (23.74 cm). In addition, Radha- 4 significantly (p value=0.03823) produced more filled grains per panicle (169) under chamber conditions than ambient condition (112). The consideration of crop variety is a good adoption measure to minimize incidence of elevated temperature in farming system.

scholarly journals Rhodamin-B increases Hippocampus cell apoptosis in Rattus norvegicus-oxidative stress related to Parkinson, Alzheimer, cancer, hyperactive, anterograde amnesia diseases

2019 ◽  
Author(s):  
Dewi Ratna Sulistina ◽  
I Wayan Arsana Wiyasa ◽  
Windhu Purnomo
Keyword(s):  
Correlation Coefficient ◽  
Rhodamine B ◽  
Rattus Norvegicus ◽  
Anterograde Amnesia ◽  
Control Group ◽  
P Value ◽  
Textile Dye ◽  
Significant Difference ◽  
The Relationship ◽  
Rhodamin B

Rhodamine B is a textile dye compounds containing chlorine (Cl-), alkylating (CH3-CH3), Poly Aromatic Hydrocarbons (PAH) which activate the enzyme cytochrome P-450 as well as the structure of quinone which is very redox that leads to the formation of Reactive Oxygen Species (ROS). ROS increases induce apoptosis of the intrinsic pathway. The imbalance ratio between BAX and BCL-2 stimulates apoptosis in Hippocampus tissue. “The selected design was “the post test only control group” using twenty-eight Wistar female Rattus norvegicus mouse age of 10-12 weeks. There was a significant difference (p-value <0.05) of total BCL-2 expression between the control group to the treatment group. Correlation coefficient of 0.945 indicates that the level of the relationship/ correlation is very strong category. Increasing doses of Rhodamine B was given, accompanied by the decrease in the expression of BCL-2. Correlation coefficient of -0.731 indicates that the level of the relationship/ correlation belongs strong category. It is concluded that Rhodamin B has been verified as capable to increase the expression of BAX and to reduce the expression of BCL-2 in hippocampus tissue on Rattus norvegicus.

scholarly journals Association of lumbar spine mobility and hamstring tightness in dental practitioners

Biomedicine ◽  
2021 ◽  
Vol 41 (1) ◽  
pp. 146-149
Author(s):  
S. Divyashri ◽  
Lavanya Prathap ◽  
S Preetha
Keyword(s):  
Lumbar Spine ◽  
Correlation Coefficient ◽  
Mean Value ◽  
P Value ◽  
Positive Correlation ◽  
Lumbar Flexion ◽  
Dental Practitioners ◽  
Significant Difference ◽  
Spine Mobility ◽  
Awkward Posture

  Introduction and Aim: The decrease in lumbar spine mobility and hamstring tightness can be caused due to the lack of regular exercise and awkward postures exposed at the workplace due to an individual's occupation. It can be expressed due to the awkward posture during dental practice as well and this can be controlled by doing regular exercises.The aim of the present study is to analyze the association of lumbar spine mobility and hamstring tightness in dental practitioners.   Materials and Methods: The study is conducted in a Private dental college and hospital for the dental practitioners who work for more than 4 hours in a day.  The hamstring tightness is measured using the goniometer and lumbar spine flexibility is measured using an inch tape. The measurements are tabulated and analyzed statistically for the correlation of lumbar spine flexibility with hamstring tightness using the SPSS tool.   Results: The collected data are analyzed statistically using correlation coefficient and the findings concluded that they have a low positive correlation. The mean value of lumbar flexion range is 19.37 cm and mean value of the hamstring tightness is 27.8°, the correlation coefficient r is 0.473. Majority of female participants have increased hamstring tightness with a maximum range of 30° and around 11% of male participants have a restriction range of 25°. Similarly, around 8% of female participants have a lumbar flexion range of around 21cm, However, there is no significant difference between the gender in both the variables with p value equals 0.35 and 0.53 (p > 0.05) respectively.    Conclusion: From the obtained results it can be concluded that there is a low positive correlation between hamstring tightness and the lumbar flexion range.  The findings suggest that the dental practitioners are prone to hamstring tightness which in turn can induce reduced lumbar spine mobility and impacts stress on the lumbar spine leading to low back pain.

Agreement Analysis of Pentacam and Lenstar in Measurement of Keratometry, Anterior Chamber Depth and Lens Thickness

2016 ◽  
Vol 1 (1) ◽  
pp. 10
Author(s):  
Azlindarita Mohd Abdullah ◽  
Norasyikin Mustafa ◽  
Loo Voon Pei ◽  
Visvaraja Subrayan
Keyword(s):  
Anterior Chamber ◽  
Correlation Coefficient ◽  
Anterior Segment ◽  
Anterior Chamber Depth ◽  
P Value ◽  
Camera System ◽  
Depth Measurement ◽  
Lens Thickness ◽  
Age Related ◽  
Significant Difference

Introduction: To assess the agreement of the Scheimpflug camera system Pentacam with the optical low-coherence reflectometry (OLCR) device LENSTAR LS900 in measuring anterior segment biometry. Methods: This is a prospective, non- randomized, comparative analysis study. Patients with age-related nucleus sclerosis cataract who fulfilled the inclusion criteria and attended the Ophthalmology Clinic in University of Malaya Medical Centre (UMMC) between December 2011 and March 2012 were recruited. The keratometer, anterior chamber (AC) depth and lens thickness were measured with both methods Pentacam and Lenstar. Results: 223 eyes of 125 patients were analysed. There was an agreement in the keratometer, anterior chamber depth and lens thickness measurement between the two devices. There was a significant correlation between K-reading as measured by both devices, at 0.05 significant level, with correlation coefficient of 0.904 and p < 0.001. The correlation coefficient of AC depth measurement between Pentacam and Lenstar at 0.05 significant level was 0.992 (p < 0.001). From the 124 eyes in which the lens thickness were measureable with both Pentacam and Lenstar, a positive correlation was noted (correlation coefficient of 0.585, p-value of < 0.001). There was no significant difference in the three parameters between males and females and among Malay, Chinese and Indian patients. Conclusions: There is a statistical agreement in the anterior segment biometry measurements between the Pentacam and the Lenstar.

Hubungan Religiusitas dengan Citra Tubuh pada Wanita Dewasa Awal

2018 ◽  
Vol 1 (1) ◽  
pp. 9-28
Author(s):  
Dessy Sumanty ◽  
Deden Sudirman ◽  
Diah Puspasari
Keyword(s):  
Body Image ◽  
Correlation Coefficient ◽  
Research Design ◽  
Calculation Result ◽  
The Body ◽  
Statistical Testing ◽  
P Value ◽  
Significance Level ◽  
Correlational Research

This research attempts to relate the body image phenomenon with the level of subject religiosity. This research used correlational research design that was involving 332 respondents. The statistical testing which is used to test the hypothesis Rank Spearman. The calculation result with the significance level of trust 95% (a = 0.05) show that the correlation coefficient is 0.083 and p-value is 0.129. It means that Ho is accepted and H1 is rejected. It can be concluded that there is no relationship between religiosity with body image.

PENENTUAN NILAI PARAMETER LIPOFILITAS SENYAWA 4-KLOROBENZOILTIOUREA DAN UJI POTENSIASI TERHADAP TIOPENTAL PADA MENCIT PUTIH (Mus musculus)

2019 ◽  
Vol 4 (1) ◽  
pp. 1
Author(s):  
Dini Kesuma
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Electronic Properties ◽  
Mus Musculus ◽  
Log P ◽  
P Value ◽  
Lead Compounds ◽  
Significant Difference ◽  
Central Nervous System Depressant ◽  
The Central Nervous System

Synthesis of the 4-chlorobenzoylthiourea compound was carried out by acylating thiourea with 4-chlorobenzoyl chloride. The 4-chlorobenzoylthiourea compound  will increase the lipophilic and the electronic properties other than the lead compounds of benzoylthiourea in order to, by expectation, raise the central nervous system depressant as well. The lipophilic would affect the ability of the compounds in penetrating biological membranes, which is highly dependent on the solubility of the drug within lipid/water. Log P is the most common method used in determining the parameter value. This experiment was to mix two dissolvents (octanol and water) which are immissible. The both levels of the compounds were carefully observed by a spectrophotometer UV-Vis. From the test, the result of log P value of the 4-chlorobenzoylthiourea compound was 2.32, while the theoretical log P value of the compounds, by using the π Hansch-Fujita method is 1.62 and the f Rekker-Mannhold method is 2.225. Consequently, the result of the test shows that there is a significant difference between the progress experiment and both theoretical log P methods. Moreover, in the test of the central nervous system depressant through the potentiation test to thiopental using mice indicates that the 4-chlorobenzoylthiourea compound have potentiation effects to thiopental compared to the lead compounds of benzoylthiourea.

Employing Personal Reading Logs through Short Stories to Enhance Students’ L2 Reading Comprehension Achievement

2019 ◽  
Vol 3 (2) ◽  
pp. 1
Author(s):  
Eliyas Sulaiman Mohandas ◽  
Nik Mastura Nik Ismail Azlan ◽  
Salwa Othman ◽  
Muhammad Aizat Azhari
Keyword(s):  
Short Stories ◽  
Reading Comprehension ◽  
P Value ◽  
L2 Reading ◽  
Reading Score ◽  
Paired Samples ◽  
Significant Difference ◽  
Post Test ◽  
The Mean ◽  
L2 Reading Comprehension

This study aims to investigate whether the use of six selected short stories throughout the duration of a 14-week course could enhance students’ reading comprehension achievement at the end of the semester. Out of the six short stories read, three were chosen as in-class assignments known as ‘Personal Reading Logs’ (hereafter, PRLs). One group of semester two Diploma students taking a reading skills course was selected through a convenience sampling method. A pre-test was conducted by having the students answer a past semester reading quiz of which the results would then be compared to their post-test (final reading exam) results. A paired samples t-test revealed no significant difference in the reading scores of the pre-test and the post-test, t (17) = -.265, p > .05. Since the p-value was bigger than 0.05, this indicated that the mean reading score of the post-test (M = 50.556) was not significantly higher than the mean reading score of the pre-test (M = 49.722). Therefore, the null hypothesis which stated that there was no difference in the mean score of the pre-test and post-test was retained. Overall, the result refuted the findings of other studies promoting the effectiveness of using short stories to enhance L2 reading comprehension achievement.

THE ANALYSIS OF NURSING STUDENT SOFT SKILLS DEVELOPMENT BETWEEN LECTURE AND PROBLEM BASED LEARNING APPROACHES 2011

2018 ◽  
Vol 1 (1) ◽  
pp. 1
Author(s):  
Hilman Syarif
Keyword(s):  
Nursing Students ◽  
Nursing Student ◽  
Soft Skills ◽  
Comparative Method ◽  
P Value ◽  
Learning Approaches ◽  
Soft Skill ◽  
Student Centered Learning ◽  
Hard Skills ◽  
Significant Difference

Introduction: Student Centered Learning (SCL) is an effective method to develop student's soft skills and hard skills which are very important to support their successful carrier later. This research was conducted to identify the differences of developed soft skills between students who learn with PBL method and lecturing method. Methods: Descriptive comparative method was used in this study. The samples consisted of 15 students who learned with PBL method and 15 students who learned with lecturing method. The sample for this research was selected by random sampling method. Results: The result showed that the average of student's soft skills score in PBL method was 122.63, while student's soft skills score in lecturing method was 116.27. Discussion & Conclusion: There was significant difference of student's soft skills in PBL method and lecturing method (p value = 0.038; α = 0.05). This study recommends nursing program management, faculty of medicine at the University of Syiah Kuala keeps PBL method running and develops other methods which facilitate hard skills and soft skills are development. Keywords: nursing students, soft skill, PBL

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