scholarly journals Distribution of Leptospira Serogroups In Swine Herds In France, 2007 - 2017

2021 ◽  
Author(s):  
Jeanne Naudet ◽  
Laurent Crespin ◽  
Julien Cappelle ◽  
Angeli Kodjo ◽  
Florence Ayral
Keyword(s):  
Retrospective Study ◽  
Selection Bias ◽  
Clinical Signs ◽  
Weighted Average ◽  
Agglutination Test ◽  
Economic Losses ◽  
Prophylactic Vaccine ◽  
Management Measures ◽  
Swine Herds ◽  
Epidemiological Information

Abstract Background: Leptospirosis is a widespread zoonotic disease caused by pathogenic Leptospira and is responsible for significant economic losses in porcine livestock. Knowledge of Leptospira serogroups and their distributions is important for evaluating the relevance of leptospirosis management measures, including the use of the prophylactic vaccine recently made available in France.A retrospective study was conducted to determine the distribution of Leptospira serogroups. Pigs from across France presenting clinical signs suggestive of leptospirosis were tested by micro-agglutination test between 2007 and 2017. We used a weighted average to determine the serogroup distributions according to the MAT results, considering cross reactions.Results: A total of 19,395 pig sera, mostly from Brittany, were tested, and 22.7 % were found to be positive for at least one Leptospira serogroup. In analysing the 4346 seropositive results for which the putative infective serogroup could be defined, we found that two serogroups out of ten were much more frequent than the others: Australis (48.5 %) and Icterohaemorrhagiae (38.2 %). Other serogroups, including Autumnalis, Panama, Ballum, Tarassovi, Sejroe, Grippotyphosa, Bataviae, and Pomona, were less common. Conclusion: Although data from diagnostic laboratories are prone to selection bias, using such a large amount of data provides a relevant overview of Leptospira distribution in space and time. Extracting epidemiological information with a standardized approach could be used for surveillance and support prophylactic strategies. Along the last decade, provide protection against the serogroups Australis and Icterohaemorrhagiae could prevent most of the clinical porcine leptospirosis in France.

scholarly journals Experimental infection of indigenous North African goats with goatpox virus

2021 ◽  
Vol 63 (1) ◽  
Author(s):  
Jihane Hamdi ◽  
Zahra Bamouh ◽  
Mohammed Jazouli ◽  
Meryem Alhyane ◽  
Najet Safini ◽  
...  
Keyword(s):  
Antibody Response ◽  
Experimental Infection ◽  
Subcutaneous Tissue ◽  
Severe Disease ◽  
Clinical Signs ◽  
Economic Losses ◽  
North African ◽  
Cutaneous Lesions ◽  
Viral Dna ◽  
Goatpox Virus

Abstract Background Goatpox is a viral disease caused by infection with goatpox virus (GTPV) of the genus Capripoxvirus, Poxviridae family. Capripoxviruses cause serious disease to livestock and contribute to huge economic losses. Goatpox and sheeppox are endemic to Africa, particularly north of the Equator, the Middle East and many parts of Asia. GTPV and sheeppox virus are considered host-specific; however, both strains can cause clinical disease in either goats or sheep with more severe disease in the homologous species and mild or sub-clinical infection in the other. Goatpox has never been reported in Morocco, Algeria or Tunisia despite the huge population of goats living in proximity with sheep in those countries. To evaluate the susceptibility and pathogenicity of indigenous North African goats to GTPV infection, we experimentally inoculated eight locally bred goats with a virulent Vietnamese isolate of GTPV. Two uninfected goats were kept as controls. Clinical examination was carried out daily and blood was sampled for virology and for investigating the antibody response. After necropsy, tissues were collected and assessed for viral DNA using real-time PCR. Results Following the experimental infection, all inoculated goats displayed clinical signs characteristic of goatpox including varying degrees of hyperthermia, loss of appetite, inactivity and cutaneous lesions. The infection severely affected three of the infected animals while moderate to mild disease was noticed in the remaining goats. A high antibody response was developed. High viral DNA loads were detected in skin crusts and nodules, and subcutaneous tissue at the injection site with cycle threshold (Ct) values ranging from 14.6 to 22.9, while lower viral loads were found in liver and lung (Ct = 35.7 and 35.1). The results confirmed subcutaneous tropism of the virus. Conclusion Clinical signs of goatpox were reproduced in indigenous North African goats and confirmed a high susceptibility of the North African goat breed to GTPV infection. A clinical scoring system is proposed that can be applied in GTPV vaccine efficacy studies.

scholarly journals Poult Enteritis and Mortality Syndrome in Turkey Poults: Causes, Diagnosis and Preventive Measures

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 2063
Author(s):  
Awad A. Shehata ◽  
Shereen Basiouni ◽  
Reinhard Sting ◽  
Valerij Akimkin ◽  
Marc Hoferer ◽  
...  
Keyword(s):  
Preventive Measures ◽  
Clinical Signs ◽  
Accurate Diagnosis ◽  
Economic Losses ◽  
Turkey Poults ◽  
Causative Agents ◽  
Enteric Disorders ◽  
And Control ◽  
Enteropathogenic Viruses ◽  
Poult Enteritis

Poult enteritis and mortality syndrome (PEMS) is one of the most significant problem affecting turkeys and continues to cause severe economic losses worldwide. Although the specific causes of PEMS remains unknown, this syndrome might involve an interaction between several causative agents such as enteropathogenic viruses (coronaviruses, rotavirus, astroviruses and adenoviruses) and bacteria and protozoa. Non-infectious causes such as feed and management are also interconnected factors. However, it is difficult to determine the specific cause of enteric disorders under field conditions. Additionally, similarities of clinical signs and lesions hamper the accurate diagnosis. The purpose of the present review is to discuss in detail the main viral possible causative agents of PEMS and challenges in diagnosis and control.

scholarly journals CRISPR/Cas9-based generation of a recombinant double-reporter pseudorabies virus and its characterization in vitro and in vivo

2021 ◽  
Vol 52 (1) ◽  
Author(s):  
Peng-Fei Fu ◽  
Xuan Cheng ◽  
Bing-Qian Su ◽  
Li-Fang Duan ◽  
Cong-Rong Wang ◽  
...  
Keyword(s):  
Pseudorabies Virus ◽  
Fluorescent Protein ◽  
Antiviral Agents ◽  
Firefly Luciferase ◽  
Inhibitory Effect ◽  
Economic Losses ◽  
Green Fluorescent ◽  
Swine Herds

AbstractPseudorabies, caused by pseudorabies virus (PRV) variants, has broken out among commercial PRV vaccine-immunized swine herds and resulted in major economic losses to the pig industry in China since late 2011. However, the mechanism of virulence enhancement of variant PRV is currently unclear. Here, a recombinant PRV (rPRV HN1201-EGFP-Luc) with stable expression of enhanced green fluorescent protein (EGFP) and firefly luciferase as a double reporter virus was constructed on the basis of the PRV variant HN1201 through CRISPR/Cas9 gene-editing technology coupled with two sgRNAs. The biological characteristics of the recombinant virus and its lethality to mice were similar to those of the parental strain and displayed a stable viral titre and luciferase activity through 20 passages. Moreover, bioluminescence signals were detected in mice at 12 h after rPRV HN1201-EGFP-Luc infection. Using the double reporter PRV, we also found that 25-hydroxycholesterol had a significant inhibitory effect on PRV both in vivo and in vitro. These results suggested that the double reporter PRV based on PRV variant HN1201 should be an excellent tool for basic virology studies and evaluating antiviral agents.

scholarly journals Experimental infection of broiler chicks with Salmonella Typhimurium from pigeon (Columba livia)

2016 ◽  
Vol 37 (4) ◽  
pp. 1919
Author(s):  
Átilla Holanda de Albuquerque ◽  
Régis Siqueira de Castro Teixeira ◽  
Débora Nishi Machado ◽  
Elisângela De Souza Lopes ◽  
Ruben Horn Vasconcelos ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Clinical Signs ◽  
Columba Livia ◽  
Poultry Feed ◽  
Economic Losses ◽  
Control Group ◽  
Broiler Chicks ◽  
Post Inoculation ◽  
Microbiological Analysis ◽  
Fecal Samples

Several cases of animal and human salmonellosis caused by the Salmonella serotype Typhimurium have been reported. In animals, subclinical infection favors pathogen dissemination through feces. In this context, the domestic pigeon (Columba livia) with an asymptomatic condition may play an important role in the transmission of salmonellosis, through the elimination of contaminated feces in commercial aviaries or in poultry feed facilities, causing economic losses to the poultry industry and presenting a risk to public health. This study aimed to evaluate the mortality, clinical signs and the presence of Salmonella Typhimurium in the feces and organs of chicks previously inoculated with bacteria isolated from a pigeon. One-day-old chicks were distributed in two experimental groups (G1 and G2) of 32 birds each, and a control group of six birds. Two inocula of 0.4 and 0.7 mL with 105 and 106 colony forming units were used in G1 and G2 birds, respectively. At 1, 4, 7 and 14 days post-inoculation (dpi) fecal samples were pooled from each cage and individual cloacal swabs were collected. At 14 dpi, all chicks were euthanized and samples were collected from the liver, spleen, lung, cecum and intestine for microbiological analysis. Mortality was only observed among G2 birds (6.25%). Most birds presented clinical signs of diarrhea at 4 dpi and no symptom as observed at 14 dpi. The results from cloacal swabs demonstrated bacterial elimination in 68.8% and 53.1% of G2 and G1 birds, respectively at 1 dpi. Additionally, fecal samples had elevated bacterial shedding in all four periods of observation , with a higher excretion at 4 dpi (62.5%) for both groups. Among G2 birds, 74.2% were positive for the pathogen in the intestine; G1 birds presented the lowest rate of lung infection (29%), and both groups had more than 50% positivity for liver and caeca. The results revealed that infected chicks with a Salmonella Typhimurium strains isolated from pigeons may host the pathogen in several organs, and simultaneously present diarrheic disorders with significant levels of bacterial excretion in feces.

scholarly journals Otitis and meningoencephalitis associated with infectious coryza (Avibacterium paragallinarum) in commercial broiler chickens

2018 ◽  
Vol 30 (5) ◽  
pp. 784-788 ◽  
Author(s):  
Manuela Crispo ◽  
C. Gabriel Sentíes-Cué ◽  
George L. Cooper ◽  
Grace Mountainspring ◽  
Charles Corsiglia ◽  
...  
Keyword(s):  
Broiler Chickens ◽  
Animal Health ◽  
Clinical Signs ◽  
Economic Losses ◽  
Laboratory System ◽  
Sequencing Analysis ◽  
Contributing Factors ◽  
Infectious Coryza ◽  
Avibacterium Paragallinarum ◽  
Commercial Broiler

Infectious coryza, caused by Avibacterium paragallinarum, is an acute respiratory disease of poultry that can result in substantial morbidity, mortality, and economic losses. In March 2017, the Turlock branch of the California Animal Health and Food Safety laboratory system encountered an unusual clinical and pathologic presentation of infectious coryza in 6 live, 29-d-old, commercial broiler chickens that were submitted for diagnostic investigation. Antemortem evaluation revealed severe neurologic signs, including disorientation, torticollis, and opisthotonos. Swollen head–like syndrome and sinusitis were also present. Histologically, severe sinusitis, cranial osteomyelitis, otitis media and interna, and meningoencephalitis were noted, explaining the clinical signs described. A. paragallinarum was readily isolated from the upper and lower respiratory tract, brain, and cranial bones. Infectious bronchitis virus (IBV) was also detected by PCR, and IBV was isolated in embryonated chicken eggs. Based on sequencing analysis, the IBV appeared 99% homologous to strain CA1737. A synergistic effect between A. paragallinarum and IBV, resulting in exacerbation of clinical signs and increased mortality, may have occurred in this case. A. paragallinarum should be considered among the possible causes of neurologic signs in chickens. Appropriate media should be used for bacterial isolation, and the role of additional contributing factors and/or complicating agents should be investigated in cases of infectious coryza.

Computed tomographic findings in 205 dogs with clinical signs compatible with middle ear disease: a retrospective study

2017 ◽  
Vol 29 (1) ◽  
pp. 45-e20 ◽  
Author(s):  
Audrey Belmudes ◽  
Charline Pressanti ◽  
Paul Y. Barthez ◽  
Eloy Castilla-Castaño ◽  
Lionel Fabries ◽  
...  
Keyword(s):  
Retrospective Study ◽  
Middle Ear ◽  
Clinical Signs ◽  
Computed Tomographic ◽  
Middle Ear Disease

scholarly journals Degenerative joint disease in cattle and buffaloes in the Amazon region: a retrospective study

2014 ◽  
Vol 34 (9) ◽  
pp. 845-850 ◽  
Author(s):  
José Diomedes Barbosa ◽  
Danillo Henrique S. Lima ◽  
Alessandra S. Belo-Reis ◽  
Cleyton P. Pinheiro ◽  
Melina G.S. Sousa ◽  
...  
Keyword(s):  
Retrospective Study ◽  
Bone Tissue ◽  
Phosphorus Deficiency ◽  
Clinical Signs ◽  
Degenerative Joint Disease ◽  
Joint Disease ◽  
Predisposing Factor ◽  
Hind Limbs ◽  
Postural Changes ◽  
Progressive Weight Loss

A retrospective study of the epidemiological and clinic-pathological aspects of cattle and buffaloes with degenerative joint disease (DJD) was conducted in the state of Pará, Brazil. From 1999 to 2014, eleven cattle and 24 buffaloes were evaluated. All the treated animals with suspected DJD underwent a clinical examination of the musculoskeletal system. In seven cattle and eight buffaloes with clinical signs of the disease postmortem examination was performed. The common clinical signs observed in both species were chronic lameness, stiff gait, postural changes, audible crackles in the affected limb, prolonged recumbency, difficulty in getting up and progressive weight loss. The lesions observed at necropsy were: irregular articular surfaces, erosion of the articular cartilage and the underlying bone tissue, and proliferation of the periarticular bone tissue with formation of osteophytes. The most affected joints in cattle and buffaloes wereof the hind limb. In buffaloes, the main predisposing factor to the onset of DJD was phosphorus deficiency. In cattle, defects of the anatomical conformation of the hind limbs, chronic trauma due to the activities performed, such as semen collection, and advanced age possibly contributed to the emergence of the disease.

scholarly journals Clinical signs, prevalence, and hematobiochemical profiles associated with Anaplasma infections in sheep of North Iraq

2020 ◽  
Vol 13 (8) ◽  
pp. 1524-1527 ◽  
Author(s):  
Donea Abdulrazak Abdullah ◽  
Fawwaz Fadhil Ali ◽  
Afrah Younis Jasim ◽  
Shola David Ola-Fadunsin ◽  
Fufa Ido Gimba ◽  
...  
Keyword(s):  
Natural Infection ◽  
Clinical Signs ◽  
Economic Losses ◽  
Nasal Discharge ◽  
Rickettsial Disease ◽  
Livestock Sector ◽  
North Iraq ◽  
Hematological And Biochemical Parameters ◽  
Biochemical Analyses ◽  
Male Sheep

Background and Aim: Anaplasma infection is a worldwide prevalent condition that causes significant economic losses in affected flocks. This study was conducted to determine the prevalence and clinical signs associated with ovine anaplasmosis as well as the hematological and biochemical changes associated with the disease in natural infection in North Iraq. Materials and Methods: A total of 420 sheep were appropriately examined, and the clinical signs were documented accordingly. Blood samples were collected and subjected to parasitological, hematological, and biochemical analyses. Results: Anaplasma-infected sheep displayed the following clinical signs: Paleness of the mucous membrane, bloody diarrhea, emaciation, pyrexia, jaundice, nasal discharge, coughing, loss of wool, nervous signs, hemoglobinuria, and lacrimation. The prevalence of Anaplasma infection was 66.19%, and female sheep were significantly (p<0.05) more infected than male sheep. The hematological and biochemical parameters were significantly different between Anaplasma-positive and Anaplasma-negative sheep. Conclusion: Anaplasma infection among sheep is a significant concern in North Iraq considering its prevalence, clinical signs, and hematological and biochemical findings, which entirely causes significant debilitating effects on sheep productivity. It is important to pay more attention toward managing tick infestation among sheep to reduce the occurrence of this rickettsial disease for a more robust livestock sector of the Iraqi economy.

scholarly journals Acute fasciolosis in cattle in southern Brazil

2013 ◽  
Vol 33 (6) ◽  
pp. 705-709 ◽  
Author(s):  
Maria de Lourdes Adrien ◽  
Ana Lucia Schild ◽  
Clairton Marcolongo-Pereira ◽  
Letícia Fiss ◽  
Jerônimo L. Ruas ◽  
...  
Keyword(s):  
Fasciola Hepatica ◽  
Abdominal Cavity ◽  
Clinical Signs ◽  
Early Spring ◽  
Eosinophil Infiltration ◽  
Coagulation Necrosis ◽  
Economic Losses ◽  
Southern Brazil ◽  
Bile Duct Proliferation ◽  
Mild Anemia

This study describes the epidemiological and pathological aspects of an outbreak of acute fasciolosis in cattle in southern Brazil. Fifteen out of 70 three-year-old pregnant cows lost weight in the 30-40 days prior to calving. Clinical signs included diarrhea, weakness, mild anemia and jaundice. Dark yellow fluid in the abdominal cavity was observed at necropsy. Fibrin and clotted blood were adhered to the pericardium and lung, primarily in the diaphragmatic lobes. The liver was enlarged, and the capsular surface was irregular with clear areas and petechiae. At the cut surface, the liver was irregular, firm and edematous, and several hemorrhagic channels could be observed. Areas of fibrosis through the parenchyma and whitish thrombi occluding the great vessels were also observed. The livers of 10 cows that not died were condemned at slaughter for lesions of fasciolosis similar to those observed at necropsy. Microscopically, the liver showed areas of coagulation necrosis, extensive hemorrhages in the streaks or foci and disruption of the parenchyma with neutrophil and eosinophil infiltration. Fibrosis and bile duct proliferation were also observed. Immature Fasciola hepatica flukes were observed in the parenchyma surrounded by degenerated hepatocytes, neutrophils, eosinophils, and hemorrhages. The outbreak occurred on a farm located in an area endemic for fasciolosis, although the acute form of the disease is not common in cattle in this region. It is likely that the cows were infected by F. hepatica metacercariae released in the late fall or early spring in the rice stubble where the herd was grazing prior to calving. Although mortality due to fasciolosis in cattle is infrequent, outbreaks can occur and treatments that are effective in both the immature and adult forms of the parasite should be administered to prevent economic losses.

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