scholarly journals Fungal α-amylase entrapped in agar-agar organic matrix “beads” enhances fabric starch-desizing potentials and α-amylase-detergent compatibility

2021 ◽  
Author(s):  
Adedeji Nelson Ademakinwa ◽  
Zainab Adenike Ayinla ◽  
Femi Kayode Agboola
Keyword(s):  
Immobilized Enzyme ◽  
Residual Activity ◽  
Organic Matrix ◽  
Industrial Applications ◽  
Free Enzyme ◽  
Detergent Additive ◽  
Detergent Compatibility ◽  
Washing Efficiency ◽  
And Control ◽  
Catalytic Cycles

Abstract Aureobasidium pullulans α-amylase (ApAmy) mixed with melted agar-agar solution and drop-wisely added to a mixture of organic solvent solution allowed for the entrapment of the α-amylase in the agar-agar organic matrix as beads. The immobilized ApAmy’s characteristics and wash performance were elucidated in comparison with the soluble ApAmy. Agar-agar at 2.0 % (w/v) and toluene: chloroform at 3:1 resulted in the highest immobilization yield retaining about 98% residual activity after ten catalytic cycles. The optimum temperature and pH for the immobilized enzyme were 60ºC and 6.5 respectively. The immobilized ApAmy hydrolysed branched and linear substrates thus establishing its broad substrate specificity. Relatively, the immobilized ApAmy (iApAmy) was more tolerant to organic solvents than the free enzyme. The iApAmy was mildly inhibited by cobalt but metals such as zinc, manganese, calcium and sodium enhanced the free and immobilized ApAmy activity. The iApAmy had a higher washing efficiency (77%) in the presence of detergents than the free enzyme (68%) and control (36%). The iApAmy showed good potentials as a detergent additive and from its characteristics, it could be useful in other industrial applications.

scholarly journals Immobilization of the Peroxygenase from Agrocybe aegerita. The Effect of the Immobilization pH on the Features of an Ionically Exchanged Dimeric Peroxygenase

Catalysts ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 560
Author(s):  
Diego Carballares ◽  
Roberto Morellon-Sterling ◽  
Xiaomin Xu ◽  
Frank Hollmann ◽  
Roberto Fernandez-Lafuente
Keyword(s):  
Anion Exchange ◽  
Immobilized Enzyme ◽  
Residual Activity ◽  
Free Enzyme ◽  
The Other ◽  
Vinyl Sulfone ◽  
Agrocybe Aegerita ◽  
Inactive Form ◽  
The Stability

This paper outlines the immobilization of the recombinant dimeric unspecific peroxygenase from Agrocybe aegerita (rAaeUPO). The enzyme was quite stable (remaining unaltered its activity after 35 h at 47 °C and pH 7.0). Phosphate destabilized the enzyme, while glycerol stabilized it. The enzyme was not immobilized on glyoxyl-agarose supports, while it was immobilized albeit in inactive form on vinyl-sulfone-activated supports. rAaeUPO immobilization on glutaraldehyde pre-activated supports gave almost quantitative immobilization yield and retained some activity, but the biocatalyst was very unstable. Its immobilization via anion exchange on PEI supports also produced good immobilization yields, but the rAaeUPO stability dropped. However, using aminated agarose, the enzyme retained stability and activity. The stability of the immobilized enzyme strongly depended on the immobilization pH, being much less stable when rAaeUPO was adsorbed at pH 9.0 than when it was immobilized at pH 7.0 or pH 5.0 (residual activity was almost 0 for the former and 80% for the other preparations), presenting stability very similar to that of the free enzyme. This is a very clear example of how the immobilization pH greatly affects the final biocatalyst performance.

Immobilized Nanoparticles-Mediated Enzymatic Hydrolysis of Cellulose for Clean Sugar Production: A Novel Approach

2019 ◽  
Vol 15 (3) ◽  
pp. 296-303 ◽  
Author(s):  
Swapnil Gaikwad ◽  
Avinash P. Ingle ◽  
Silvio Silverio da Silva ◽  
Mahendra Rai
Keyword(s):  
Catalytic Activity ◽  
Enzymatic Hydrolysis ◽  
Immobilized Enzyme ◽  
Free Enzyme ◽  
Magnetic Nanomaterials ◽  
Sugar Production ◽  
Cellulase Enzyme ◽  
Enzymatic Hydrolysis Of Cellulose ◽  
Hydrolysis Of Cellulose ◽  
Hydrolysis Of

Background: Enzymatic hydrolysis of cellulose is an expensive approach due to the high cost of an enzyme involved in the process. The goal of the current study was to apply magnetic nanomaterials as a support for immobilization of enzyme, which helps in the repeated use of immobilized enzyme for hydrolysis to make the process cost-effective. In addition, it will also provide stability to enzyme and increase its catalytic activity. Objective: The main aim of the present study is to immobilize cellulase enzyme on Magnetic Nanoparticles (MNPs) in order to enable the enzyme to be re-used for clean sugar production from cellulose. Methods: MNPs were synthesized using chemical precipitation methods and characterized by different techniques. Further, cellulase enzyme was immobilized on MNPs and efficacy of free and immobilized cellulase for hydrolysis of cellulose was evaluated. Results: Enzymatic hydrolysis of cellulose by immobilized enzyme showed enhanced catalytic activity after 48 hours compared to free enzyme. In first cycle of hydrolysis, immobilized enzyme hydrolyzed the cellulose and produced 19.5 ± 0.15 gm/L of glucose after 48 hours. On the contrary, free enzyme produced only 13.7 ± 0.25 gm/L of glucose in 48 hours. Immobilized enzyme maintained its stability and produced 6.15 ± 0.15 and 3.03 ± 0.25 gm/L of glucose in second and third cycle, respectively after 48 hours. Conclusion: This study will be very useful for sugar production because of enzyme binding efficiency and admirable reusability of immobilized enzyme, which leads to the significant increase in production of sugar from cellulosic materials.

Commissioning and Control of the AMB Supported 3.5 kW Laboratory Gas Blower Prototype

2013 ◽  
Vol 198 ◽  
pp. 451-456 ◽  
Author(s):  
Rafał P. Jastrzębski ◽  
Alexander Smirnov ◽  
Katja Hynynen ◽  
Janne Nerg ◽  
Jussi Sopanen ◽  
...  
Keyword(s):  
Control System ◽  
Permanent Magnet ◽  
Induction Motor ◽  
Industrial Applications ◽  
Magnetic Bearing ◽  
Active Magnetic Bearing ◽  
Sensor Calibration ◽  
Full Potential ◽  
Disturbance Analysis ◽  
And Control

This paper presents the practical results of the design analysis, commissioning, identification, sensor calibration, and tuning of an active magnetic bearing (AMB) control system for a laboratory gas blower. The presented step-by-step procedures, including modeling and disturbance analysis for different design choices, are necessary to reach the full potential of the prototype in research and industrial applications. The key results include estimation of radial and axial disturbance forces caused by the permanent magnet (PM) rotor and a discussion on differences between the unbalance forces resulting from the PM motor and the induction motor in the AMB rotor system.

scholarly journals Nanotechnology: A Journey towards Finding Solutions

2015 ◽  
Vol 5 (1) ◽  
pp. 61
Author(s):  
Satya Pal Singh
Keyword(s):  
Health And Safety ◽  
Functional Materials ◽  
Simulation Method ◽  
Industrial Applications ◽  
Policy Makers ◽  
Advance Research ◽  
American Physical ◽  
Richard Feynman ◽  
And Control ◽  
Thin Film Magnetism

<p class="1Body">Nanotechnology is the understanding and control of matter at the diemnsions ranging between 1-100 nm. One nanometer is one billionth of a meter. Nanotechnology involves manipulation of atoms, imaging, measuring and modelling at nano scale. Its potentials were first highlighted by Richard Feynman in the American Physical Scociety meeting in 1959. Though, he did not coin the world nanotechnology himself but he explored the possiblities of functional materials at the bottom of the scale. In last two decades this technology has been commercialized to great extent and gaining importance day by day influencing the economies of different countries and henceforth enforcing the policy makers to address the issues like environment, health and safety. Governments are regularisaing and monitoring its research, uses, applications and technology transfer which includes intelluctaul property rights. This paper addresses the dimensions and trends of nanotechnology covering economic aspects. The paper is focussed on the changes in the functional properties of nanomaterials as physical, chemical, optical, electronic, electrical, magnetic etc. in comparision to those of the bulk of material. It has been discussed how the basic and advance research in nanoscience could be explotiedfor making technologies for its commercial and industrial applications for the benefit and safety of the soceity. Thin film magnetism is demonstrated using Monte Carlo simulation method. Experimental synthesisof some of thenanorods and qunatum dots are also discussed.</p>

scholarly journals Immobilization of Mutant Phosphotriesterase on Fuller’s Earth Enhanced the Stability of the Enzyme

Catalysts ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 983
Author(s):  
Wahhida Latip ◽  
Victor Feizal Knight ◽  
Ong Keat Khim ◽  
Noor Azilah Mohd Kasim ◽  
Wan Md Zin Wan Yunus ◽  
...  
Keyword(s):  
Thermal Stability ◽  
Metal Ions ◽  
Half Life ◽  
Industrial Applications ◽  
Free Enzyme ◽  
High Tolerance ◽  
Tween 40 ◽  
Fuller’S Earth ◽  
The Stability ◽  
Biochemical Features

Immobilization is a method for making an enzyme more robust in the environment, especially in terms of its stability and reusability. A mutant phosphotriesterase (YT PTE) isolated from Pseudomonas dimunita has been reported to have high proficiency in hydrolyzing the Sp and Rp-enantiomers of organophosphate chromophoric analogs and therefore has great potential as a decontamination agent and biosensor. This work aims to investigate the feasibility of using Fuller’s earth (FE) as a YT PTE immobilization support and characterize its biochemical features after immobilization. The immobilized YT PTE was found to show improvement in thermal stability with a half-life of 24 h compared to that of the free enzyme, which was only 8 h. The stability of the immobilized YT PTE allowed storage for up to 4 months and reuse for up to 6 times. The immobilized YT PTE showed high tolerance against all tested metal ions, Tween 40 and 80 surfactants and inorganic solvents. These findings showed that the immobilized YT PTE became more robust for use especially with regards to its stability and reusability. These features would enhance the future applicability of this enzyme as a decontamination agent and its use in other suitable industrial applications.

scholarly journals The Development of Radio and 3G Based Telemetry System for the Remote Gas Accounting and Control Nodes

2019 ◽  
Vol 8 (9S3) ◽  
pp. 1417-1421
Keyword(s):  
Dynamic Range ◽  
High Dynamic Range ◽  
Industrial Applications ◽  
Telemetry System ◽  
Computing Device ◽  
Gas Leakage ◽  
Remote Environment ◽  
High Dynamic ◽  
And Control ◽  
Direct Management

In this article we have described the use of vortex and recently developed ultrasonic flowmeters with high dynamic range of 1 to 1500 for industrial applications. Its software and the software of corresponding computing device is able to avoid gas leakage, to minimize energy consumption and to save human resources while maintaining metrological data. Described is the low power consumption that makes it possible to use this ultrasonic flowmeter in hard remote environment without direct management for a period of several months. Shown is the new telemetry system that was developed to unite flowmeters in the severe conditions of the desert with power supply problems and low GPRS signal quality. Experiments held in Turkmenistan have shown that device indications didn’t drift and remained stable during the year, that is a great advantage in comparison to rotary and turbine flowmeters. Also described is the mobile ultrasonic calibration stand that uses the same physical principles and similar software. Outlined is the usage of modern wireless technologies to collect and transmit metrological data.

scholarly journals The Use of Granular Cyclopentanone as Alternative to Artificial Source of Carbon Dioxide in Improved Passive Outdoor Host Seeking Device (POHD)

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Stella T. Kessy ◽  
Bruno A. Nyundo ◽  
Ladslaus L. Mnyone ◽  
Issa N. Lyimo
Keyword(s):  
Electrical Power ◽  
Scale Up ◽  
Residual Activity ◽  
Yeast Fermentation ◽  
Power Sources ◽  
Host Seeking ◽  
Relative Attractiveness ◽  
Residual Malaria Transmission ◽  
Control Devices ◽  
And Control

Reliable sources of CO2 that are relatively cheap, obtainable, and easy to sustain are immediately required for scaling up of odor-baited mosquito surveillance and control devices. Several odor-baited devices are in the pipeline; however, their scale-up against residual malaria transmission, particularly in resource poor areas, is limited by the unavailability of reliable sources of CO2 and reliance on electrical power sources among other factors. We evaluated the use of granular cyclopentanone as an alternative to artificial or yeast fermentation-produced CO2 in passive outdoor host seeking device (POHD). Experiments were conducted against semifield reared An. arabiensis within the semifield system (SFS) at Ifakara Health Institute. Mosquitoes were tested against odor-baited POHDs augmented with yeast fermentation-produced CO2, granular cyclopentanone, attractive blends (Mbita or Ifakara), or their combinations. An insecticide, bendiocarb, was a killing agent used as a proxy for marking the mosquitoes visit the POHDs. Relative attractiveness of different treatment combinations was compared based on the proportion of dead mosquitoes that visited the POHD. The POHD augmented with granules of cyclopentanone alone was attractive to An. arabiensis as much as, or more than, POHDs augmented with yeast fermentation-produced CO2. The POHD baited with CO2 attracted more mosquitoes than those POHDs baited with synthetic blends alone; when these blends are combined with CO2, they attracted more mosquitoes than individual blends. More importantly, such POHDs baited with cyclopentanone attracted far greater proportion of mosquitoes than the POHD baited with either Mbita or Ifakara blend alone. The granular cyclopentanone strongly enhanced/potentiated the attractiveness of POHD baited with Mbita blends against mosquitoes compared to that of POHD baited with Ifakara blend. Moreover, the granular cyclopentanone retained its residual activity against An. arabiensis for up to 2 months after application particularly when used in combination with Mbita blend. In conclusion, this study demonstrates that cyclopentanone granules have the potential to substitute sources of CO2 in outdoor-based surveillance and control devices, thus warranting evaluation of such alternative under realistic field conditions.

Design of a Compliant Industrial Gripper Driven by a Bistable Shape Memory Alloy Actuator

2020 ◽  
Author(s):  
Dominik Scholtes ◽  
Stefan Seelecke ◽  
Gianluca Rizzello ◽  
Paul Motzki
Keyword(s):  
Shape Memory ◽  
High Energy ◽  
Industrial Applications ◽  
High Energy Density ◽  
Small Scale ◽  
Monitoring And Control ◽  
Actuation System ◽  
First Results ◽  
Functional Prototype ◽  
And Control

Abstract Within industrial manufacturing most processing steps are accompanied by transporting and positioning of workpieces. The active interfaces between handling system and workpiece are industrial grippers, which often are driven by pneumatics, especially in small scale areas. On the way to higher energy efficiency and digital factories, companies are looking for new actuation technologies with more sensor integration and better efficiencies. Commonly used actuators like solenoids and electric engines are in many cases too heavy and large for direct integration into the gripping system. Due to their high energy density shape memory alloys (SMA) are suited to overcome those drawbacks of conventional actuators. Additionally, they feature self-sensing abilities that lead to sensor-less monitoring and control of the actuation system. Another drawback of conventional grippers is their design, which is based on moving parts with linear guides and bearings. These parts are prone to wear, especially in abrasive environments. This can be overcome by a compliant gripper design that is based on flexure hinges and thus dispenses with joints, bearings and guides. In the presented work, the development process of a functional prototype for a compliant gripper driven by a bistable SMA actuation unit for industrial applications is outlined. The focus lies on the development of the SMA actuator, while the first design approach for the compliant gripper mechanism with solid state joints is proposed. The result is a working gripper-prototype which is mainly made of 3D-printed parts. First results of validation experiments are discussed.

scholarly journals Enhancing the Ethynylation Performance of CuO-Bi2O3 Nanocatalysts by Tuning Cu-Bi Interactions and Phase Structures

Catalysts ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 35 ◽  
Author(s):  
Zhipeng Wang ◽  
Zhuzhu Niu ◽  
Quanai Hao ◽  
Lijun Ban ◽  
Haitao Li ◽  
...  
Keyword(s):  
Industrial Applications ◽  
Strong Interactions ◽  
Catalytic Systems ◽  
Key Factor ◽  
Different Temperatures ◽  
Catalytic Stability ◽  
Phase Spinel ◽  
Co Precipitation ◽  
Catalytic Cycles ◽  
Cu Ions

Catalytic systems consisting of copper oxide and bismuth oxide are commonly employed for the industrial production of 1,4-butynediol (BD) through ethynylation. However, few studies have investigated the influence mechanism of Bi for these Cu-based catalysts. Herein, a series of nanostructured CuO-Bi2O3 catalysts were prepared by co-precipitation followed by calcination at different temperatures. The obtained catalysts were applied to the ethynylation reaction. The textural and crystal properties of the catalysts, their reduction behavior, and the interactions between copper and bismuth species, were found to strongly depend on temperature. When calcined at 600 °C, strong interactions between Cu and Bi in the CuO phase facilitated the formation of highly dispersed active cuprous sites and stabilized the Cu+ valency, resulting in the highest BD yield. Bi2O3 was completely absent when calcined at 700 °C, having been converted into the spinel CuBi2O4 phase. Spinel Cu2+ was released gradually to form active Cu+ species over eight catalytic cycles, which continuously replenished the decreasing activity resulting from the formation of metallic Cu and enhanced catalytic stability. Moreover, the positive correlation between the in-situ-formed surface Cu+ ions and BD yield suggests that the amount of Cu+ ions is the key factor for ethynylation of formaldehyde to BD on the as prepared CuO-Bi2O3 catalysts. Based on these results and the literature, we propose an ethynylation reaction mechanism for CuO-based catalysts and provide a simple design strategy for highly efficient catalytic CuO-Bi2O3 systems, which has considerable potential for industrial applications.

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