scholarly journals Community structure, distribution pattern, and influencing factors of soil Archaea in the construction area of a large-scale photovoltaic power station

2021 ◽  
Author(s):  
Wei Wu ◽  
bo yuan ◽  
Shengjuan Yue ◽  
Penghui Zou ◽  
Ruoting Yang ◽  
...  
Keyword(s):  
Community Structure ◽  
Environmental Factors ◽  
Distribution Pattern ◽  
Archaeal Community ◽  
Community Diversity ◽  
Power Station ◽  
Photovoltaic Power ◽  
Significant Difference ◽  
Bare Land ◽  
Archaeal Communities

Abstract The photovoltaic power station in Qinghai has been built for 8 years, however, its impact on the regional soil ecological environment has not been studied in depth. To reveal the structure and distribution pattern of archaeal communities in desert soil under the influence of a large photovoltaic power station, a comparative study was carried out between the soil affected by photovoltaic panels and the bare land samples outside the photovoltaic station in Gonghe, Qinghai Province. The abundance, community structure, diversity, and distribution characteristics of archaea were analyzed by quantitative PCR and Illumina-MiSeq high-throughput sequencing, and the main environmental factors affecting the variation of soil archaeal community were identified by RDA. The contribution rate of environmental factors and human factors to microbial community diversity was quantitatively evaluated by VPA. The results showed that there was no significant difference in soil nutrients and other physicochemical factors between the photovoltaic power station and bare land. Thaumarchaeota was the dominant archaeal phylum in the area, accounting for more than 99% of archaeal phylum, while at the level of genus, Nitrososphaera was the dominant archaeal genera. There was no significant difference in archaeal community structure between and under different types of PV panels. The analysis has shown that the construction of a photovoltaic station has little effect on the community structure of soil archaea in a desert area, and it was speculated that the selection of niche played a leading role in the distribution pattern of soil archaeal community. This study provides the basis for a scientific understanding of the characteristics and distribution patterns of soil archaeal communities affected by the construction of a photovoltaic power station.

scholarly journals Deciphering the archaeal communities in tree rhizosphere of the Qinghai-Tibetan Plateau

2020 ◽  
Author(s):  
Mengjun Zhang ◽  
Liwei Chai ◽  
Muke Huang ◽  
Weiqian Jia ◽  
Jiabao Guo ◽  
...  
Keyword(s):  
Community Structure ◽  
Tibetan Plateau ◽  
Keystone Species ◽  
Terrestrial Ecosystem ◽  
Archaeal Community ◽  
Ammonium Nitrogen ◽  
Bulk Soil ◽  
Available Phosphorus ◽  
Picea Crassifolia ◽  
Archaeal Communities

Abstract Background: The Qinghai-Tibetan Plateau represents one of the most important component of the terrestrial ecosystem and a particularly vulnerable region, which harbouring complex and diverse microbiota. The knowledge about their underground microorganisms have largely been studied, but the characteristics of rhizosphere microbiota, particularly archaeal communities remains unclear. Results: High-throughput Illumina sequencing was used to investigate the rhizosphere archaeal communities of two native alpine trees (Picea crassifolia and Populus szechuanica) living on the Qinghai-Tibetan Plateau. The archaeal community structure in rhizospheres significantly differed from that in bulk soil. Thaumarchaeota was the dominant archaeal phylum in all soils tested (92.46-98.01%), while its relative abundance in rhizospheres were significantly higher than that in bulk soil. Ammonium nitrogen, soil organic matter, available phosphorus and pH were significantly correlated with the archaeal community structure, and the deterministic processes dominated the assembly of archaeal communities across all soils. In addition, the network structures of the archaeal community in the rhizosphere were less complex than they were in the bulk soil, and an unclassified archaeal group (Unclassified_k_norank) was identified as the keystone species in all archaeal networks. Conclusions: Overall, the structure, assembly and co-occurrence patterns of archaeal communities are significantly affected by the presence of roots of alpine trees living on the Qinghai-Tibetan Plateau. This study provides new insights into our understanding of archaeal communities in vulnerable ecosystems.

scholarly journals Changes in the Microbial Community Diversity of Oil Exploitation

Genes ◽  
2019 ◽  
Vol 10 (8) ◽  
pp. 556 ◽  
Author(s):  
Jingjing Liu ◽  
Jing Wu ◽  
Jiawei Lin ◽  
Jian Zhao ◽  
Tianyi Xu ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Environmental Factors ◽  
Oil Recovery ◽  
Microbial Community Structure ◽  
Storage Tank ◽  
Oil Production ◽  
Operational Taxonomic Unit ◽  
Community Diversity ◽  
Microbial Resources

To systematically evaluate the ecological changes of an active offshore petroleum production system, the variation of microbial communities at several sites (virgin field, wellhead, storage tank) of an oil production facility in east China was investigated by sequencing the V3 to V4 regions of 16S ribosomal ribonucleic acid (rRNA) of microorganisms. In general, a decrease of microbial community richness and diversity in petroleum mining was observed, as measured by operational taxonomic unit (OTU) numbers, α (Chao1 and Shannon indices), and β (principal coordinate analysis) diversity. Microbial community structure was strongly affected by environmental factors at the phylum and genus levels. At the phylum level, virgin field and wellhead were dominated by Proteobacteria, while the storage tank had higher presence of Firmicutes (29.3–66.9%). Specifically, the wellhead displayed a lower presentence of Proteobacteria (48.6–53.4.0%) and a higher presence of Firmicutes (24.4–29.6%) than the virgin field. At the genus level, the predominant genera were Ochrobactrum and Acinetobacter in the virgin field, Lactococcus and Pseudomonas in the wellhead, and Prauseria and Bacillus in the storage tank. Our study revealed that the microbial community structure was strongly affected by the surrounding environmental factors, such as temperature, oxygen content, salinity, and pH, which could be altered because of the oil production. It was observed that the various microbiomes produced surfactants, transforming the biohazard and degrading hydro-carbon. Altering the microbiome growth condition by appropriate human intervention and taking advantage of natural microbial resources can further enhance oil recovery technology.

scholarly journals Microbial Community Structure Drives Predatory Myxobacteria Distribution Under Different Compost Manures

2021 ◽  
Author(s):  
Wei Dai ◽  
Ning Wang ◽  
Wenhui Wang ◽  
Xianfeng Ye ◽  
Zhongli Cui ◽  
...  
Keyword(s):  
Community Structure ◽  
High Throughput Sequencing ◽  
Soil Microbial Communities ◽  
Alpha Diversity ◽  
Distribution Patterns ◽  
Diversity Indices ◽  
Community Diversity ◽  
Microbial World ◽  
Significant Difference ◽  
Compost Manure

Abstract Myxobacteria are unique predatory microorganisms with a distinct social lifestyle. The associated taxa play key roles in the microbial food webs in different ecosystems and regulate the community structures of soil microbial communities. Compared with conditions under conventional management, under organic conditions, myxobacteria abundance increases in the soil, which could be related to the presence of abundant myxobacteria in the applied compost manure. In the present study, high-throughput sequencing technologies were used to investigate the distribution patterns and drivers of predatory myxobacteria community distribution patterns in four common compost manures. According to the results, there was a significant difference in predatory myxobacteria community structure among different compost manure treatments (P < 0.05). The alpha-diversity indices of myxobacteria community under swine manure compost were the lowest (Observed OTU richness = 13.25, Chao1 = 14.83, Shannon = 0.61), and those under wormcast were the highest (Observed OTU richness = 30.25, Chao1 = 31.65, Shannon = 2.62). Bacterial community diversity and Mg2+ and Ca2+ concentrations were the major factors influencing myxobacteria distribution patterns under different compost manure treatments. In addition, pH, total nitrogen, and organic carbon influenced myxobacteria distribution in compost manure. The predator–prey relationship between prey bacteria and myxobacteria and the interaction between myxobacteria and specific bacterial taxa (Micrococcales) in compost manure could explain the influence of bacteria on myxobacteria community structure. Further investigations on the in-situ distribution patterns of predatory myxobacteria and the key bacteria influencing their distribution are would advance our understanding of the ecological distribution patterns and functions of predatory microorganisms in the microbial world.

scholarly journals Spatial-temporal dynamics and influencing factors of archaeal communities in the sediments of Lancang River cascade reservoirs (LRCR), China

PLoS ONE ◽  
2021 ◽  
Vol 16 (6) ◽  
pp. e0253233
Author(s):  
Bo Yuan ◽  
Wei Wu ◽  
Mengjing Guo ◽  
Xiaode Zhou ◽  
Shuguang Xie
Keyword(s):  
Community Structure ◽  
16S Rrna ◽  
Large Scale ◽  
Temporal Dynamics ◽  
Archaeal Community ◽  
Rrna Gene ◽  
Regulated Rivers ◽  
Cascade Reservoirs ◽  
Lancang River ◽  
Archaeal Communities

The spatial and temporal distribution of the archaeal community and its driving factors in the sediments of large-scale regulated rivers, especially in rivers with cascade hydropower development rivers, remain poorly understood. Quantitative PCR (qPCR) and Illumina MiSeq sequencing of the 16S rRNA archaeal gene were used to comprehensively investigate the spatiotemporal diversity and structure of archaeal community in the sediments of the Lancang River cascade reservoirs (LRCR). The archaeal abundance ranged from 5.11×104 to 1.03×106 16S rRNA gene copies per gram dry sediment and presented no temporal variation. The richness, diversity, and community structure of the archaeal community illustrated a drastic spatial change. Thaumarchaeota and Euryyarchaeota were the dominant archaeal phyla in the sediments of the cascade rivers, and Bathyarchaeota was also an advantage in the sediments. PICRUSt metabolic inference analysis revealed a growing number of genes associated with xenobiotic metabolism and carbon and nitrogen metabolism in downstream reservoirs, indicating that anthropogenic pollution discharges might act as the dominant selective force to alter the archaeal communities. Nitrate and C/N ratio were found to play important roles in the formation of the archaeal community composition. In addition, the sediment archaeal community structure was also closely related to the age of the cascade reservoir and hydraulic retention time (HRT). This finding indicates that the engineering factors of the reservoir might be the greatest contributor to the archaeal community structure in the LRCR.

scholarly journals Community Structure and Abundance of Archaea in a Zostera marina Meadow: A Comparison between Seagrass-Colonized and Bare Sediment Sites

Archaea ◽  
2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Pengfei Zheng ◽  
Chuantao Wang ◽  
Xiaoli Zhang ◽  
Jun Gong
Keyword(s):  
Community Structure ◽  
Zostera Marina ◽  
High Throughput Sequencing ◽  
Archaeal Community ◽  
Distribution Patterns ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Absolute Abundance ◽  
Bare Sediment ◽  
Archaeal Communities

Seagrass colonization alters sediment physicochemical properties by depositing seagrass fibers and releasing organic carbon and oxygen from the roots. How this seagrass colonization-induced spatial heterogeneity affects archaeal community structure and abundance remains unclear. In this study, we investigated archaeal abundance, diversity, and composition in both vegetated and adjacent bare surface sediments of a Zostera marina meadow. High-throughput sequencing of 16S rDNA showed that Woesearchaeota, Bathyarchaeota, and Thaumarchaeota were the most abundant phyla across all samples, accounting for approximately 42%, 21%, and 17% of the total archaeal communities, respectively. In terms of relative abundance, Woesearchaeota and Bathyarchaeota were not significantly different between these two niches; however, specific subclades (Woese-3, Woese-21, Bathy-6, Bathy-18) were significantly enriched in vegetated sediments (P<0.05), while Thaumarchaeota was favored in unvegetated sites (P=0.02). The quantification of archaeal 16S rRNA genes showed that the absolute abundance of the whole archaeal community, Bathyarchaeota, and Woese-3, Woese-10, Woese-13, and Woese-21 was significantly more abundant in vegetated sediments than in bare sediments (P<0.05). Our study expands the available knowledge of the distribution patterns and niche preferences of archaea in seagrass systems, especially for the different subclades of Woesearchaeota and Bathyarchaeota, in terms of both relative proportions and absolute quantities.

scholarly journals Bryophyte communities and seston in a karst stream (Jankovac Stream, Papuk Nature Park, Croatia)

2012 ◽  
Vol 48 (1) ◽  
pp. 125-138 ◽  
Author(s):  
Maria Špoljar ◽  
Tvrtko Dražina ◽  
Ana Ostojić ◽  
Marko Miliša ◽  
Marija Gligora Udovič ◽  
...  
Keyword(s):  
Organic Matter ◽  
Community Structure ◽  
Environmental Factors ◽  
Environmental Parameters ◽  
Community Diversity ◽  
Nature Park ◽  
Community Assemblages ◽  
Abundance And Diversity ◽  
Bottom Cover ◽  
Source Site

An investigation into bryophyte communities in karst Jankovac Stream (Papuk Nature Park, Croatia) was carried out once a month from July 2008 to June 2009. Samples were taken from two lotic microhabitats: (i) Jankovac Spring (JS), a hypocrenal habitat with dense bryophyte clusters (90% bottom cover) and (ii) Jankovac Waterfall (JW), with scattered bryophyte clusters (50% bottom cover). At the same time, seston samples were collected during the spring as the source site and after the JW, as the outflow site. The goals of this study were to understand the (i) algal, protozoan and metazoan bryophyte community assemblages in these two lotic microhabitats, (ii) influence of environmental factors on the structuring of the bryophyte community and (iii) structure of seston along the longitudinal profile. A total of 172 taxa were determined: 68 algae, 55 protozoa, 24 meiofauna and 25 macroinvertebrates. Statistically significant differences between two microhabitats differing in percentage of bryophyte cover were established testing 13 environmental parameters. In dense bryophyte clusters, community structure was determined by flow velocity and pH, and macroinvertebrates achieved higher diversity and abundance. On the contrary, in scattered bryophyte coverage algae, protozoa and meiofauna reached higher abundance and diversity governed by the amount of suspended organic matter and epiphyton. In contrast to previous studies, the inverse ratio of community diversity and abundanceversuspercentage of bryophyte cover was established. We assume this to be the result of an enrichment of the scattered bryophyte clusters by upstream seston. Additionally, the effects of anthropogenic hydromorphological disturbance are reflected in macroinvertebrate diversity and abundance reduction.

scholarly journals Seasonal Dynamics of Bathyarchaeota-Dominated Benthic Archaeal Communities Associated with Seagrass (Zostera japonica) Meadows

2021 ◽  
Vol 9 (11) ◽  
pp. 1304
Author(s):  
Pengyuan Liu ◽  
Haikun Zhang ◽  
Zenglei Song ◽  
Yanyan Huang ◽  
Xiaoke Hu
Keyword(s):  
Community Structure ◽  
Seasonal Changes ◽  
Archaeal Community ◽  
Rrna Gene ◽  
Zostera Japonica ◽  
Total Organic Nitrogen ◽  
Seagrass Meadows ◽  
Metabolic Functions ◽  
Seagrass Ecosystems ◽  
Archaeal Communities

Little is known about the seasonal dynamic of archaeal communities and their potential ecological functions in temperate seagrass ecosystems. In this study, seasonal changes in diversity, community structure, and potential metabolic functions of benthic archaea in surface sediments of two seagrass meadows along the northern Bohai Sea in China were investigated using Miseq sequencing of the 16S rRNA gene and Tax4Fun2 functional prediction. Overall, Crenarchaeota (mainly Bathy-15, Bathy-8, and Bathy-6) dominated, followed by Thermoplasmatota, Asgardarchaeota, and Halobacterota, in terms of alpha diversities and relative abundance. Significant seasonal changes in the entire archaeal community structure were observed. The major phyla Methanobacteria, Nitrosopumilales, and genus Methanolobus had higher proportions in spring, while MBG-D and Bathyarchaeota were more abundant in summer and autumn, respectively. Alpha diversities (Shannon and Simpson) were the highest in summer and the lowest in autumn (ANOVA test, p < 0.05). Salinity, total organic carbon, and total organic nitrogen were the most significant factors influencing the entire archaeal community. Higher cellulose and hemicellulose degradation potentials occurred in summer, while methane metabolism potentials were higher in winter. This study indicated that season had strong effects in modulating bsenthic archaeal diversity and functional potentials in the temperate seagrass ecosystems.

scholarly journals Land conversion changes soil microbial community structure and diversity

2020 ◽  
Author(s):  
Tong Zhang ◽  
Yufei Liu ◽  
Xin Sui ◽  
Fuqiang Song
Keyword(s):  
Land Use ◽  
Community Structure ◽  
Microbial Communities ◽  
Soil Microbial Communities ◽  
Community Diversity ◽  
Forest Land ◽  
Soil Microbial Community Structure ◽  
Soil Microbial ◽  
Significant Difference ◽  
The Impact

Abstract Background: To study the impact of land-use change on soil microbial community structure and diversity in Northeast China, three typical land-use types (plough, grassland, and forest), from grassland change to forest land and grassland change to plough, in the Qiqihar region of Heilongjiang Province were taken as research objects. Methods: MiSeq high-throughput sequencing technology based on bacterial 16S rRNA and fungal ITS rRNA was used to study the above community structure of soil bacteria and fungi and to explore the relationship between soil bacteria and soil environmental factors. Results: The results showed that there was no significant difference in soil bacterial community diversity and fungal community diversity after the grassland was completely changed to forest land. The dominant bacterial phylum changed from Actinobacteria to Acidobacteria, the dominant fungal phylum changed from Ascomycetes to Basidiomycetes, and the ECM functional group increased significantly. After the grassland was changed to plough, there was no significant difference in the diversity of soil bacterial communities, and the diversity of fungal communities increased significantly. The dominant phylum changed from Actinomycetes to Proteobacteria. The dominant phylum was still Ascomycetes, and the functional groups of pathogens and parasites increased significantly. CCA showed that soil pH, MC, NO3--N, TP and AP were important factors affecting the composition of soil microbial communities, and changes in land-use patterns changed the physical and chemical properties of soils, thereby affecting the structure and diversity of microbial communities. Conclusions: Our research results clarify the impact of changes in land use on the characteristics of soil microbial communities and provide basic data on the healthy use of land.

scholarly journals Axial Differences in Community Structure ofCrenarchaeota and Euryarchaeota in the Highly Compartmentalized Gut of the Soil-Feeding TermiteCubitermes orthognathus

2001 ◽  
Vol 67 (10) ◽  
pp. 4880-4890 ◽  
Author(s):  
Michael W. Friedrich ◽  
Dirk Schmitt-Wagner ◽  
Tillmann Lueders ◽  
Andreas Brune
Keyword(s):  
Community Structure ◽  
Archaeal Community ◽  
Small Subunit ◽  
Ssu Rdna ◽  
Ecological Niches ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Rdna Sequences ◽  
Electron Sink ◽  
Archaeal Communities

ABSTRACT Methanogenesis represents an important electron sink reaction in the hindgut of soil-feeding termites. This is the first comprehensive analysis of the archaeal community structure within the highly compartmentalized intestinal tract of a humivorous insect, combining clonal analysis and terminal restriction fragment (T-RF) length polymorphism (T-RFLP) fingerprinting of the archaeal communities in the different gut compartments of Cubitermes orthognathus. We found that the morphological and physicochemical heterogeneity of the gut is reflected in a large phylogenetic diversity and pronounced axial differences in the composition of the archaeal gut microbiota, notably among those clones or ribotypes that could be assigned to methanogenic taxa. Comparative analysis of the relative frequencies of different archaeal lineages among the small-subunit rRNA gene (SSU rDNA) clones and their corresponding T-RF indicated that the archaeal community in the anterior, extremely alkaline hindgut compartment (P1) consists mainly of members of theMethanosarcinaceae, whereasMethanobacteriaceae andMethanomicrobiales predominate in the subsequent, more posterior compartments (P3/4a and P4b). The relative abundance ofThermoplasmales increased towards the rectum (P5). SSU rDNA sequences representing Crenarchaeota, which have not yet been reported to occur in the intestinal tracts of arthropods, were detected in all gut sections. We discuss how the spatial distribution of methanogenic populations may be linked to axial heterogeneity in the physicochemical gut conditions and to functional adaptations to their respective ecological niches.

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