scholarly journals Applying Four-Steps Characteristic Ion Filtering with HPLC-QE Orbitrap MS Approach to Rapid Characterize Compound Structure s and Determine Major Representative Components in Modified Tabusen-2 Decoction

2021 ◽  
Author(s):  
yu Zhao ◽  
xin Dong ◽  
qianxi Feng ◽  
rui Dong ◽  
ren Bu ◽  
...  
Keyword(s):  
Quantitative Method ◽  
Structural Features ◽  
Assay Method ◽  
Compound Structure ◽  
Material Basis ◽  
Development And Utilization ◽  
Good Repeatability ◽  
Main Components ◽  
Orbitrap Ms

Abstract Background: Modified Tabusen-2 decoction (MTBD) is Traditional Chinese Mongolia Medicine, it is consist of “Lancitou” “Duzhong” “Sanqi” and “Honghua”. MTBD is mainly used to treat osteoporosis. However, the precise material basis of this prescription is not yet fully elucidated, which affects its modernization developmentMethods: We establish a new HPLC-QE Orbitrap MS technology with four-steps characteristic ion filtering (FSCIF) strategy to quickly and effectively identify the structural features of MTBD, and determine the representative compounds content. Then the quantitative method of nineteen main components in MTBD were built, and eight batches MTBD were determined as well. Results: By using the FSCIF strategy, a total of 143 compounds were unambiguously or tentatively identified, including 5 compounds were first reported in MTBD. Nineteen representative components were simultaneously quantified by HPLC-QE Orbitrap MS, and it is suitable for eight batches of MTBD. Methodology analysis showed that the assay method of 19 compounds (including three pairs of isomers) with a good repeatability, accuracy, and stability. Conclusion: The method established above was successfully applied to assess the quality of MTBD extracts, which prove that the concentrations of 19 compounds varied significantly in different batches of MTBD extracts. Collectively, our findings enhance our molecular understanding of the MTBD formulation and will allow us to control its quality in a better way. At the same time, this study can promote the development and utilization of ethnic medicine.

scholarly journals Applying Four-Step Characteristic Ion Filtering with HPLC-Q-Exactive MS/MS Spectrometer Approach for Rapid Compound Structures Characterization and Major Representative Components Quantification in Modified Tabusen-2 Decoction

2021 ◽  
Vol 2021 ◽  
pp. 1-20
Author(s):  
Yu Zhao ◽  
Xin Dong ◽  
Zhi Wang ◽  
Rui Dong ◽  
Ren Bu ◽  
...  
Keyword(s):  
Mass Spectrum ◽  
Neutral Loss ◽  
Structural Features ◽  
Assay Method ◽  
Material Basis ◽  
Development And Utilization ◽  
Q Exactive ◽  
Good Repeatability ◽  
Accuracy And Stability

Modified Tabusen-2 decoction (MTBD) is traditional Chinese Mongolia medicine, mainly used to treat osteoporosis. However, the precise material basis of this prescription is not yet fully elucidated. Herein, we establish an HPLC-Q-Exactive MS/MS spectrometer method with four-step characteristic ion filtering (FSCIF) strategy to quickly and effectively identify the structural features of MTBD and determine the representative compounds content. The FSCIF strategy included database establishment, characteristic ions summarization, neutral loss fragments screening, and secondary mass spectrum fragment matching four steps. By using this strategy, a total of 143 compounds were unambiguously or tentatively annotated, including 5 compounds which were first reported in MTBD. Nineteen representative components were simultaneously quantified with the HPLC-Q-Exactive MS/MS spectrometer, and it is suitable for eight batches of MTBD. Methodology analysis showed that the assay method had good repeatability, accuracy, and stability. The method established above was successfully applied to assess the quality of MTBD extracts. Collectively, our findings enhance our molecular understanding of the MTBD formulation and will allow us to control its quality in a better way. At the same time, this study can promote the development and utilization of ethnic medicine.

Histamine metabolism disorder in pathogenesis of chronic pelvic pain in patients with external genital endometriosis

2017 ◽  
pp. 56-60
Author(s):  
М.Р. Оразов ◽  
В.Е. Радзинский ◽  
М.Б. Хамошина ◽  
Е.Н. Носенко ◽  
Э.С. Токаева ◽  
...  
Keyword(s):  
Quality Of Life ◽  
Pelvic Pain ◽  
Peripheral Blood ◽  
Chronic Pelvic Pain ◽  
Pain Syndrome ◽  
Histamine Level ◽  
Life Assessment ◽  
Assay Method ◽  
Histamine Metabolism

Цель исследования - изучение особенностей обмена гистамина при хронической тазовой боли у пациенток с наружным генитальным эндометриозом. Методика. В сыворотке крови 100 пациенток методом высокоточного (в нг/мл) иммуноферментного твердофазного анализа определяли концентрацию гистамина. Использованы реактивы «Histamine ЕLISA», аппарат BAE-1000 Гистамин (Labor Diagnostika Nord - LDN, Германия). Оценка выраженности болевого синдрома осуществлялась по шкале ВАШ, оценка качества жизни - на основании опросника для оценки качества жизни при эндометриозе (EHR-30), оценка тревожности - опросника Спилбергера-Ханина. Результаты. Показано статистически значимое повышение уровня гистамина у пациенток с высокой интенсивностью болевого синдрома. Женщины с наружным генитальным эндометриозом, сопровождающимся интенсивным болевым синдромом в 100% случаев обнаруживали высокие уровни ситуативной и личностной тревожности, тогда как, лишь у 40% женщин (n = 16) контрольной группы выявлен умеренный уровень тревожности. Депрессивные расстройства выявлены у 58,3% женщин с хронической тазовой болью (n = 35), из них основную часть (n = 20) составили женщины с выраженной степенью болевого синдрома по ВАШ. Заключение. Психоэмоциональное состояние женщин с тазовой болью, ассоциированной с наружным генитальным эндометриозом, характеризуется высокими уровнями депрессивных и тревожных расстройств, значительным снижением уровня качества жизни. Выявлена прямая связь между интенсивностью болевого синдрома и уровнем гистамина в периферической крови пациенток с наружным генитальным эндометриозом. Objective. To study features of histamine metabolism in patients with chronic pelvic pain associated with external genital endometriosis. Methods. For quantitative assessment of histamine level in peripheral blood was taken from 100 patients which than was centrifuged. In blood serum histamine concentration was determined by enzyme-linked immunosorbent assay method with reagents «Histamine ЕLISA» on the machine BAE-1000 Histamine (Labor Diagnostika Nord - LDN, Hermany). A pain syndrome was assessed by Visual Analog Scale (VAS), quality of life assessment - by Endometriosis Health Profile Questionnaire (EHR-30), level of anxiety was determined by Spielberger-Khanin questionnaire. The results. Showed statistically higher histamine level in patients with severe pain according to VAS. After assessment of results obtained from Spielberger-Khanin questionnaire 100% experimental group’s women with external genital endometriosis (n = 60) were noted to be have high level of state and trait anxiety, then 40% women of control group (n = 16) have moderate level of anxiety. The incidence of depression in women with chronic pelvic pain was 58.3% (n = 35) and the main part (n = 20) were women with severe stage of pelvic pain according to VAS. Conclusions. Psycho emotional condition of women with external genital endometriosis associated pelvic pain characterized by higher depression and anxiety levels, with significant decrease quality of life. Direct relationship also was found between pain syndrome intensity and histamine level in peripheral blood in patients with external genital endometriosis.

Methodical approach to the assessment of the educational migration environment of the population of the Carpathian region

2019 ◽  
pp. 37-48
Author(s):  
Lyubov Semiv
Keyword(s):  
Institutional Conditions ◽  
Carpathian Region ◽  
Research Activities ◽  
Methodical Approach ◽  
Synthetic Indicator ◽  
Industry Collaboration ◽  
Migration Potential ◽  
Educational Migration ◽  
Main Components

The role and importance of the educational migration environment in activating migration movements of the population is described. The main components of the educational migration environment of the population are identified, and their features are outlined. Indicators have been proposed and the conditions for the formation of the educational migration environment of the population have been determined. It is proved that «freedom of knowledge movement» motivates students, teachers and researchers to combine educational and research activities with future employment abroad. The processes of educational migration in the form of cross-border education and academic mobility are presented. The concept of educational migration environment is defined and five main components of its formation are described: quantitative measurement of educational migration potential; quality of the academic environment; motivational conditions; opportunities for universities and industry collaboration in research; institutional conditions in the educational sphere. The list of indicators offered by the Ukrainian statistics is provided for quantitative representation of each component of the educational migration. Based on the method of multidimensional (cluster) analysis, the regional index of formation of educational migration environment is calculated. Using this method allows to move from the assessment of educational migration environment on 28 indicators to the construction of one synthetic indicator. Application of methodical approach allows to see the place of the region by the important parameters of development of the environment of educational migration of the population, to evaluate the attractiveness, opportunities and threats of formation of this environment in the regional dimension. It is proved that the «most favorable» environment in the Carpathian region has the Lviv region (4th place in Ukraine). Other regions of the Ukrainian Carpathians occupy in the ranking the lower places: respectively Ivano-Frankivsk (15th place), Chernivtsi (21st place), Transcarpathian region (24th place).

New Monitoring Strategy for the Quality Control in the Processing Practice of Scutellariae Radix

2020 ◽  
Vol 16 (3) ◽  
pp. 303-311
Author(s):  
Qi Huang ◽  
Chunsong Cheng ◽  
Lili Li ◽  
Daiyin Peng ◽  
Cun Zhang
Keyword(s):  
Quality Control ◽  
Monitoring Strategy ◽  
Scutellariae Radix ◽  
Chromatographic Fingerprints ◽  
New Strategy ◽  
Processed Products ◽  
Main Components ◽  
Processing Techniques ◽  
Multivariate Monitoring

Background: Scutellariae Radix (Huangqin) is commonly processed into 3 products for different clinical applications. However, a simple analytical method for quality control has rarely been reported to quickly estimate the degree of processing Huangqin or distinguish differently processed products or unqualified Huangqin products. Objective: To study a new strategy for quality control in the processing practice of Huangqin. Methods: Seven kinds of flavonoids that mainly exist in Huangqin were determined by HPLC-DAD. Chromatographic fingerprints were established to study the variation and discipline of the 3 processed products of Huangqin. PCA and OPLS-DA were used to classify differently processed products of Huangqin. Results: The results showed that baicalin and wogonoside were the main components in the crude and the alcohol Huangqin herb while baicalein and wogonin mainly existed in carbonized Huangqin. The results of mathematical statistics revealed that the processing techniques can make the quality of medicinal materials more uniform. Conclusion: This multivariate monitoring strategy is suitable for quality control in the processing of Huangqin.

scholarly journals Determination of Environmental Factors for the Implementation of the Exploitability Index in Industrial Aggregate Mining Using Multi-Criteria Analysis

2021 ◽  
Vol 10 (4) ◽  
pp. 196
Author(s):  
Julio Manuel de Luis-Ruiz ◽  
Benito Ramiro Salas-Menocal ◽  
Gema Fernández-Maroto ◽  
Rubén Pérez-Álvarez ◽  
Raúl Pereda-García
Keyword(s):  
Environmental Factors ◽  
Environmental Factor ◽  
Human Life ◽  
Accurate Estimation ◽  
Analytic Hierarchy ◽  
Main Components ◽  
Hierarchy Process ◽  
Made In

The quality of human life is linked to the exploitation of mining resources. The Exploitability Index (EI) assesses the actual possibilities to enable a mine according to several factors. The environment is one of the most constraining ones, but its analysis is made in a shallow way. This research is focused on its determination, according to a new preliminary methodology that sets the main components of the environmental impact related to the development of an exploitation of industrial minerals and its weighting according to the Analytic Hierarchy Process (AHP). It is applied to the case of the ophitic outcrops in Cantabria (Spain). Twelve components are proposed and weighted with the AHP and an algorithm that allows for assigning a normalized value for the environmental factor to each deposit. Geographic Information Systems (GISs) are applied, allowing us to map a large number of components of the environmental factors. This provides a much more accurate estimation of the environmental factor, with respect to reality, and improves the traditional methodology in a substantial way. It can be established as a methodology for mining spaces planning, but it is suitable for other contexts, and it raises developing the environmental analysis before selecting the outcrop to be exploited.

scholarly journals Analysis of ergonomic and unergonomic human lifting behaviors by using Inertial Measurement Units

2017 ◽  
Vol 3 (1) ◽  
pp. 7-10 ◽  
Author(s):  
Jan Kuschan ◽  
Henning Schmidt ◽  
Jörg Krüger
Keyword(s):  
Quality Of Life ◽  
Machine Learning ◽  
Angular Velocity ◽  
Musculoskeletal System ◽  
Occupational Diseases ◽  
Inertial Measurement Units ◽  
Inertial Measurement ◽  
Measurement Units ◽  
Main Components

Abstract:This paper presents an analysis of two distinct human lifting movements regarding acceleration and angular velocity. For the first movement, the ergonomic one, the test persons produced the lifting power by squatting down, bending at the hips and knees only. Whereas performing the unergonomic one they bent forward lifting the box mainly with their backs. The measurements were taken by using a vest equipped with five Inertial Measurement Units (IMU) with 9 Dimensions of Freedom (DOF) each. In the following the IMU data captured for these two movements will be evaluated using statistics and visualized. It will also be discussed with respect to their suitability as features for further machine learning classifications. The reason for observing these movements is that occupational diseases of the musculoskeletal system lead to a reduction of the workers’ quality of life and extra costs for companies. Therefore, a vest, called CareJack, was designed to give the worker a real-time feedback about his ergonomic state while working. The CareJack is an approach to reduce the risk of spinal and back diseases. This paper will also present the idea behind it as well as its main components.

Research on the Optimal Design Method of the Main Components for the Wheelchair Pickup

2013 ◽  
Vol 791-793 ◽  
pp. 799-802
Author(s):  
Ya Ping Wang ◽  
H.R. Shi ◽  
L. Gao ◽  
Z. Wang ◽  
X.Y. Jia ◽  
...  
Keyword(s):  
Parametric Design ◽  
Design Method ◽  
Basic Function ◽  
Design Parameters ◽  
Algorithm Optimization ◽  
Optimization Analysis ◽  
Research Designs ◽  
Optimal Design Method ◽  
Main Components

With the increasing of the aging of population all over the world, and With the inconvenience coming from diseases and damage, there will be more and more people using the wheelchair as a tool for transport. When it cant be short of the wheelchair in the daily life, the addition of the function will bring the elevation of the quality of life for the unfortunate. Staring with this purpose, the research designs a pickup with planetary bevel gear for the wheelchair. After determining the basic function of the wheelchair aids, the study determines the design parameters by using the knowledge of parametric design and completes the model for the system with Pro/E, on the other hand, it completes key components optimization analysis which is based on genetic algorithm optimization.

Modal Analysis and Optimization of Spindle Box of Turn-Milling Center Based on Finite Element Method

2012 ◽  
Vol 226-228 ◽  
pp. 281-284
Author(s):  
Li Da Zhu ◽  
Xiao Bang Wang ◽  
Tiao Biao Yu ◽  
Wan Shan Wang
Keyword(s):  
Finite Element ◽  
Natural Frequencies ◽  
Element Model ◽  
Ansys Software ◽  
Design Requirement ◽  
Design Quality ◽  
Machining Center ◽  
Main Components ◽  
Turn Milling

The dynamic characteristics of machine tool may directly affect its machining capability, which is analyzed to improve the machining precision and efficiency. In this paper, the 3D finite element model of main components turn-milling center is established by using ANSYS software, and then spindle box of turn-milling center is analyzed and optimized; the natural frequencies and vibration models are obtained after analysis, which guarantee the design requirement of the machining center. Therefore it is significant to improve the design quality of machining center by using FEA software in the design process.

ISSUES OF ETHNOCULTURAL TRADITIONS DEVELOPMENT IN MODERN ARTISTIC PRACTICE

2020 ◽  
Vol 1 (4) ◽  
pp. 50-54
Author(s):  
K. K. Murataev ◽  
◽  
S. M. Krykbayeva ◽  
Keyword(s):  
Folk Art ◽  
Structural Features ◽  
Artistic Practice ◽  
Creative Development ◽  
Traditional Art ◽  
Spatial Features ◽  
Applied Art ◽  
Main Components ◽  
Structurally Stable ◽  
Figurative Interpretation

The article deals with insufficiently studied theoretical aspects of revealing and analyzing new elements and motives of artistic and compositional means of expression in folk art, as well as topical issues concerning determining principles and possible options for their creative development in modern decorative and applied art, design, and artistic practice in general. The most important structural features of shaping in traditional art in the ontologically interrelated system "nature — man — object" are analyzed. The main structurally stable elements and components in Kazakh folk ornament are distinguished and characterized, these are the circle, S-shaped element, cruciform and triangular components. The evolution of their ideological and figurative interpretation and symbolism is traced, and their role in the genesis of folk art traditions formed over thousands of years is revealed. The selected basic elements of folk ornament, as the main components of means of artistic expression, are proposed to be defined and developed in line with modern interpretations of artistic and aesthetic categories — dynamics, statics, harmony and their decorative variations in accordance with the volumetric and spatial features of form.

A Semantic Differential for Evaluating the Sound Quality of Fan Systems

2017 ◽  
Author(s):  
Carolin Feldmann ◽  
Thomas Carolus ◽  
Marc Schneider
Keyword(s):  
Principal Component ◽  
Semantic Differential ◽  
Sound Quality ◽  
Heat Pumps ◽  
First Case ◽  
Objective Metrics ◽  
Main Components ◽  
Air Conditioning Systems ◽  
The University

Fans are main components e.g. in heating, ventilating and air conditioning systems for vehicles or buildings, cooling units of engines and electronic circuits, and household appliances such as kitchen exhaust hoods or vacuum cleaners. End-users increasingly demand a high sound quality of their system or device. The overall objective of a recent research project at the University of Siegen is a multidimensional assessment of fan sound quality. In a first step an advanced novel semantic differential for the assessment of fan-related sounds is established with the aid of carefully designed jury tests. Eventually, this semantic differential is employed for sound quality jury tests of fans in kitchen exhaust hoods, heat pumps and air purifiers as a first case. Finally, a prediction model is suggested, which relates the outcome from the jury tests to objective metrics. A principal component analysis is carried out and yields five main assessment criteria with 23 relevant adjective scales. The results show that the perceived sound quality of fan systems is mainly determined by the loudness and tonality of the sound. The spectral content (represented by the sharpness) as well as the time structure (represented by the roughness) have no significant impact on perceived sound quality of the fan systems investigated.

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