scholarly journals Ellagic Acid (EA), a Tannin Was Isolated From Eucalyptus Citriodora Leaves and Its Anti-Inflammatory Activity

2021 ◽  
Author(s):  
Qiujian Yu ◽  
Zongcai Feng ◽  
Liping Huang ◽  
Jingwei He ◽  
Zhongliu Zhou ◽  
...  
Keyword(s):  
Gastric Ulcer ◽  
Intragastric Administration ◽  
Eucalyptus Citriodora ◽  
H Nmr ◽  
Tnf Α ◽  
Liver And Kidney ◽  
Anti Inflammatory ◽  
Significant Protective Effect ◽  
Ulcer Treatment ◽  
C Nmr

Abstract A tannin, EA ( 1 ), and other nine non-tannins compounds, gallic acid ( 2 ), quercetin ( 3 ), myricetin ( 4 ), 3-O-methylellagic acid 4'-O-α-L-rhamnopyranoside ( 5 ), quercetin-3-O-β-D-galactopyranoside ( 6 ), kaempferol-3-O-β-D-glucoside ( 7 ), quercetin 3-O-β-D-glucuronide ( 8 ), quercetin-3-O-rutinoside ( 9 ), 3,3',4-tri-O-methylellagic acid-4'-O-β-D-glucopyranosyl ( 10 ) were isolated from a valuable medicinal plant, Eucalyptus citriodora . Structural identification of these compounds was conducted using 1 H NMR and 13 C NMR spectroscopy and comparing their spectral data with those previously reported in literatures. The anti-inflammatory effects of EA were evaluated in ethanol-induced acute gastric ulcer mice models in our study. The result demonstrated that the intragastric administration of EA significantly prevented the gastric ulceration caused by ethanol treatments. Especially, the gastric tissue in the middle dose EA (100 mg/kg) showed few ulcerations with only slight focal congestion which indicated that it has a significant protective effect on gastric ulcer by increasing the IL-10 and PGE 2 levels, and reducing the IL-6, TNF-α, GAS and COX-2 levels. In addition, the middle-dose EA has no adverse effect on liver and kidney. These findings imply that EA exerts gastroprotective effects by means of its anti-inflammatory effects and may be a potential drug for anti-ulcer treatment.

scholarly journals The potential effects of Centella asiatica ethanolic extracts as an anti-inflammatory agent through decreasing TNF-α expression in indomethacin-induced gastric ulcer model rats

2021 ◽  
Author(s):  
Priscilla Maiselina Sriepindonnta ◽  
Fatimah Nur Fitriani ◽  
Savannah Quila Thirza ◽  
Made Dinda Pratiwi ◽  
Dwi Evan Prima Putra Noviardi ◽  
...  
Keyword(s):  
Gastric Ulcer ◽  
Centella Asiatica ◽  
Inflammatory Agent ◽  
Tnf Α ◽  
Ethanolic Extracts ◽  
Ulcer Model ◽  
Anti Inflammatory

Evaluation of Antinociceptive and Anti-Inflammatory Activities of a New Triterpene Saponin from Bauhinia variegata Leaves

2009 ◽  
Vol 64 (11-12) ◽  
pp. 798-808 ◽  
Author(s):  
Mona A. Mohamed ◽  
Madeha R. Mammoud ◽  
Heiko Hayen
Keyword(s):  
High Resolution ◽  
Spectral Data ◽  
Cinnamic Acid ◽  
Cinnamic Acid Derivatives ◽  
Triterpene Saponin ◽  
H Nmr ◽  
Antischistosomal Activity ◽  
Bauhinia Variegata ◽  
Anti Inflammatory ◽  
C Nmr

A new triterpene saponin, named as 23-hydroxy-3α-[O-α-L-1C4-rhamnopyranosyl-(1´´4´)- O-α-L-4C1-arabinopyranosyl-oxy]olean-12-en-28-oic acid O-α-L-1C4-rhamnopyranosyl- (1´´´´´→4´´´´)-O-β-D-4C1-glucopyranosyl-(1´´´´→6´´´)-O-β-D-4C1-glucopyranosyl ester (9), was isolated from the leaves of Bauhinia variegata Linn. In addition, six flavonoid compounds along with two cinnamic acid derivatives were isolated and identified based on their chromatographic properties, and chemical and spectral data (ESI-high resolution-MSn, 1H NMR, 13C NMR, 1H-1H COSY, HSQC, and HMBC). Compound 9 was found to be nontoxic (LD50) and to have significant anti-inflammatory and antinociceptive activities. It also showed a slight antischistosomal activity.

Novel benzofurane-pyrazole derivatives with anti-inflammatory, cyclooxygenase inhibitory and cytotoxicity evaluation

2022 ◽  
Vol 0 (0) ◽  
Author(s):  
Zafer Sahin ◽  
Yağmur Özhan ◽  
Hande Sipahi ◽  
Sevde Nur Biltekin ◽  
Leyla Yurttaş ◽  
...  
Keyword(s):  
Hydroxy Ketone ◽  
No Production ◽  
Raw 264.7 ◽  
Pyrazole Derivatives ◽  
H Nmr ◽  
Pyrazolone Derivatives ◽  
Cox 2 ◽  
Anti Inflammatory ◽  
Cox 1 ◽  
C Nmr

Abstract Novel benzofurane-pyrazolone hybrids have been synthesized for evaluating their anti-inflammatory and cytotoxic properties. 4-(2-chloroacetyl)-1,5-dimethyl-2-phenyl-1,2-dihydro-3H-pyrazol-3-one were reacted with α-hydroxy aldehyde or α-hydroxy ketone derivatives to obtain nine novel pyrazolone derivatives. Structures were successfully elucidated by 1H NMR, 13C NMR, IR and HRMS. Enzyme inhibitory activity was measured on cyclooxygenases (COXs) as considered to address anti-inflammatory activity. Compound 2 showed the highest activity on both COX-1 and COX-2 subtypes with 12.0 μM and 8.0 μM IC50, respectively. This activity was found close to indomethacin COX-2 inhibition measured as 7.4 μM IC50. Rest of the compounds (1, 3–9) showed 10.4–28.1 μM IC50 on COX-2 and 17.0–35.6 μM IC50 on COX-1 (Compound 1 has no activity on COX-1). Tested compounds (1–9) showed activity on NO production. Only compound was the 4, which showed a low inhibition on IL-6 levels. Cell viability was up to 60% at 100 μM for all compounds (1–9) on RAW 264.7 and NIH3T3 cell lines, thus compounds were reported to be noncytotoxic.

scholarly journals Moxibustion Ameliorates Ovarian Reserve in Rats by Mediating Nrf2/HO-1/NLRP3 Anti-Inflammatory Pathway

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Ge Lu ◽  
Qian Wang ◽  
Zi-Jing Xie ◽  
Shang-Jie Liang ◽  
Hong-Xiao Li ◽  
...  
Keyword(s):  
Ovarian Reserve ◽  
Tissue Injury ◽  
Ovarian Tissue ◽  
Hormone Replacement ◽  
Receptor Protein ◽  
Heme Oxygenase 1 ◽  
Intragastric Administration ◽  
Related Factor ◽  
Tnf Α ◽  
Anti Inflammatory

Diminished ovarian reserve (DOR) is an increasingly emerging reproductive disorder that disturbs reproductive-aged women, which is closely linked with inflammation. In clinic, moxibustion has already been applied for reproductive problems. In the present study, we examined the involvement of inflammation in DOR and investigated the effect of moxibustion for its anti-inflammatory activities. Methods. DOR rat model was established using tripterygium glycosides A tablets (TGs) suspension by intragastric administration and was then treated with either moxibustion or hormone replacement therapy (HRT), respectively. Estrus cycles were observed through vaginal cytology. Ovarian morphological alterations were observed by HE staining. The serum levels of follicle-stimulating hormone (FSH), estradiol (E2), anti-Müllerian hormone (AMH), tumor necrosis factor alpha (TNF-α), and interleukin-10 (IL-10) were measured through ELISA. The expression levels of Nrf2, HO-1, and NLRP3 were detected using immunohistochemistry. Nrf2, HO-1, and NLRP3 mRNA were examined by RT-PCR. Results. Moxibustion improved estrus cycles, FSH, E2, and AMH levels relative to DOR rats as well as HRT, while also inhibiting ovarian tissue injury. Anti-inflammatory cytokine IL-10 in peripheral blood was upregulated, and proinflammatory factor TNF-α was decreased after treatment with moxibustion. Moxibustion enhanced the expression of mRNA and protein of nuclear factor erythroid 2-related factor (Nrf2) and heme oxygenase-1 (HO-1); in the mean time, nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) was suppressed. Conclusions. We demonstrated that moxibustion could ameliorate the ovarian reserve in rats induced by TGs. Overall, the effect of moxibustion was comparable to that of HRT. The underlying mechanism could be attributed to the anti-inflammatory effects of moxibustion, which suppressed NLRP3 activation by upregulating Nrf2/HO-1 signaling pathway.

Anti-Inflammatory Effects of Boldine and Reticuline Isolated from Litsea cubeba through JAK2/STAT3 and NF-κB Signaling Pathways

Planta Medica ◽  
2017 ◽  
Vol 84 (01) ◽  
pp. 20-25 ◽  
Author(s):  
Xinyao Yang ◽  
Xiaoli Gao ◽  
Yuan Cao ◽  
Qiang Guo ◽  
Shanshan Li ◽  
...  
Keyword(s):  
Synergistic Effects ◽  
Underlying Mechanism ◽  
Janus Kinase ◽  
Inflammatory Activity ◽  
Intragastric Administration ◽  
Litsea Cubeba ◽  
Tnf Α ◽  
Anti Inflammatory ◽  
Rat Paw

AbstractThe anti-inflammatory effects of boldine and reticuline isolated from Litsea cubeba were evaluated by using xylene-induced ear edema and carrageenan-induced paw edema in mice and rats. Our results demonstrated that intragastric administration with boldine and reticuline significantly mitigated ear weight in mice and decreased paw volume in rats. A combination administration of boldine (0.5 mg/kg) + reticuline (0.25 mg/kg) resulted in a potentiated inhibition in these two models. In parallel, boldine or reticuline reduce the infiltration of neutrophil leukocytes in rat paw tissue, respectively, and the combination of the two groups performed a better anti-inflammatory activity as shown in histopathologies. Boldine, reticuline, and their combination notably inhibited mRNA expressions of TNF-α, and IL-6 and reduced the phosphorylation levels of Janus kinase 2 (JAK2) and signal transducer and activator of transcription 3 (STAT3). Beyond that, their combination also can reduce the phosphorylation levels of p65 and IκBα in the pathological tissues of animals, as observed by real-time PCR and western blot analyses, respectively. These findings indicate for the first time that boldine and reticuline have not only anti-inflammatory activity but also potential synergistic effects in vivo. The underlying mechanism may relate to the inhibition on the expression of pro-inflammatory cytokines, such as TNF-α and IL-6, which may be a consequence of JAK2/STAT3 and NF-κB pathway involvements. This study provides useful data for further exploration and application of boldine and reticuline as potential anti-inflammatory medicines.

scholarly journals Potential Toxicity Evaluation of Protopine in Macleaya cordata (Willd.) R. Br.—A Bioactivity Guided Approach

2021 ◽  
Vol 8 ◽  
Author(s):  
Wanjun Hu ◽  
Fan Yang ◽  
Weixue Liu ◽  
Liyang Guo ◽  
Liwen Ai ◽  
...  
Keyword(s):  
Primary Component ◽  
Perennial Herb ◽  
Bioactive Constituents ◽  
Apparent Lack ◽  
H Nmr ◽  
Liver And Kidney ◽  
Macleaya Cordata ◽  
Kidney Liver ◽  
The Brain ◽  
C Nmr

Macleaya cordata (Willd.) R. Br. (M. cordata) is a perennial herb known for its chemotherapeutic properties, strong feeding additive, and potential antidiarrheal drug. Despite its therapeutic potentials, its clinical applications are hindered by an apparent lack of toxicity data. In this study, the toxic ingredients of this plant were investigated using a bioactivity-guided approach. Two compounds, protopine and allocryptopine, were purified and elucidated by LC-MS, 1H-NMR, and 13C-NMR. Protopine, a primary component in M. cordata, had an LD50 of 313.10 mg/kg i.e., which was considered toxic. An autopsy was performed on protopine-administered mice, and the histopathology of the kidney, liver, brain, heart, lung, and spleen was determined. Autopsy findings included hemorrhage in the respiratory system, lung congestion, and hemorrhage and edema in the parenchymatous organs (heart, liver, kidney, and brain). Histopathology confirmed the pathological changes in the brain, liver, and kidney. Protopine is one of the principal bioactive constituents of many phytopreparations used in veterinary and human medicine, such as Sangrovit and Iberogast. Our findings indicated that phytopreparations containing protopine might pose a serious health threat to humans and animals.

scholarly journals Rosmarinic Acid Enriched Fraction fromPerilla frutescensLeaves Strongly Protects Indomethacin-Induced Gastric Ulcer in Rats

2019 ◽  
Vol 2019 ◽  
pp. 1-13 ◽  
Author(s):  
Napapan Kangwan ◽  
Komsak Pintha ◽  
Suree Lekawanvijit ◽  
Maitree Suttajit
Keyword(s):  
Body Weight ◽  
Gastric Ulcer ◽  
Rosmarinic Acid ◽  
Perilla Frutescens ◽  
Gastric Ulcers ◽  
Histopathological Study ◽  
Intragastric Administration ◽  
Standard Drug ◽  
Aqueous Fraction ◽  
Anti Inflammatory

Gastric ulcers are a common problem in upper gastrointestinal tract (GI) disorders. Nonsteroidal anti-inflammatory drugs (NSAIDs) are one of the most aggressive factors leading to inducing gastric ulcers. Natural products with lower toxicity and safety are currently sought as a potential source to minimize the effect of the gastric ulcers.Perilla frutescensor Nga-mon (in Thai) leaves are rich in rosmarinic acid (RA), which has antioxidant, anti-inflammatory, and anticancer effects. This study investigates the protective effect of ethanolic extract (EE) and aqueous fraction (AF) fromPerilla frutescensleaves, which are rich in RA, on indomethacin- (IND-) induced gastric ulcer in a rat model. The EE at the doses of 50 and 500 mg/kg body weight, AF at the doses of 50, 250, and 500 mg/kg body weight, or famotidine (a standard drug) were administered for 14 days prior to ulcer induction. The ulceration was performed by intragastric administration of IND. Gross gastric ulcers and biological and histological parameters were examined. The pretreatment with AF had more significant effects than EE, including reduced ulcer index, decreased gastric secretion volume and decreased acidity, but it had an elevated gastric pH relative to the IND-induced gastric ulcer. In a histopathological study, the EE and AF decreased mucosal ulcer, inflammatory infiltration, and degenerative lining cells. The IND-induced expression of inflammatory mediators was significantly attenuated with EE and AF. The experiment also remarkably showed the preservation of mucus and apoptosis protection of EE and AF on a gastric mucosal ulcer. The findings demonstrated that the EE and AF of perilla leaves were capable of protecting the stomach against gastric ulcers induced by IND through anti-inflammatory and antiapoptotic mechanisms that should be further investigated. It is suggested thatPerilla frutescensleaf could be a potential alternative source of RA as a therapeutic agent and food supplement for NSAID-induced gastric injuries.

scholarly journals Difference of Liver and Kidney Metabolic Profiling in Chronic Atrophic Gastritis Rats between Acupuncture and Moxibustion Treatment

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Qi-da He ◽  
Yuan-peng Huang ◽  
Li-bing Zhu ◽  
Jia-cheng Shen ◽  
Lin-yu Lian ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Atrophic Gastritis ◽  
Metabolic Profiling ◽  
Chronic Atrophic Gastritis ◽  
Intragastric Administration ◽  
Therapeutic Effects ◽  
H Nmr ◽  
Acupuncture And Moxibustion ◽  
Liver And Kidney ◽  
Electroacupuncture Treatment

Acupuncture and moxibustion proved to be very effective in chronic atrophic gastritis (CAG). According to the Chinese traditional medicine theory, chronic diseases have an influence on the function of liver and kidney. However, there is little research to demonstrate this theory. This study is aimed at assessing the1H NMR-based metabolic profiling in liver and kidney of CAG rats and comparing the difference between electroacupuncture and moxibustion treatment. Male SD rats were subjected to CAG modeling by intragastric administration of mixture of 2% sodium salicylate and 30% alcohol coupled with compulsive sporting and irregular fasting for 12 weeks and then treated by electroacupuncture or moxibustion at Liangmen (ST 21) and Zusanli (ST 36) acupoints for 2 weeks. A1H NMR analysis of liver and kidney samples along with histopathological examination and molecular biological assay was employed to assess and compare the therapeutic effects of electroacupuncture and moxibustion. CAG brought characterization of metabolomic signatures in liver and kidney of rats. Both electroacupuncture and moxibustion treatment were found to normalize the CAG-induced changes by restoring energy metabolism, neurotransmitter metabolism, antioxidation metabolism, and other metabolism, while the moxibustion treatment reversed more metabolites related to energy metabolism in liver than electroacupuncture treatment. CAG did have influence on liver and kidney of rats. Both of these treatments had good effects on CAG by reversing the CAG-induced perturbation in liver and kidney. For regulating the energy metabolism in liver, the moxibustion played more important role than electroacupuncture treatment.

Anti-inflammatory potential of chia seeds oil and mucilage against adjuvant-induced arthritis in obese and non-obese rats

2020 ◽  
Vol 31 (4) ◽  
Author(s):  
Doha A. Mohamed ◽  
Rasha S. Mohamed ◽  
Karem Fouda
Keyword(s):  
Oxidative Stress ◽  
Lipid Profile ◽  
Sprague Dawley ◽  
Pressing Method ◽  
Adjuvant Induced Arthritis ◽  
Freeze Dried ◽  
Tnf Α ◽  
Obese Rats ◽  
Liver And Kidney ◽  
Anti Inflammatory

AbstractBackgroundNatural anti-inflammatory nutraceuticals may be useful in suppressing the incessant aggravation of rheumatoid arthritis. Chia seeds as a natural source of antioxidants help prevent several oxidative stress-mediated diseases. The current study was focused on arthritis combined with obesity and evaluated the validation of oil and mucilage extracted from chia seeds as anti-inflammatory nutraceuticals in obese and non-obese adjuvant arthritic rat model.MethodsChia seeds oil was extracted by pressing method, whereas the mucilage was extracted using water (50 °C for 30 min). Oil and freeze-dried mucilage were tested for their anti-inflammatory effects using 48 male Sprague-Dawley rats. Obesity was developed in rats after 8 weeks of feeding on high-fat high-sucrose diet; on the first day of the ninth week, chia seeds oil and mucilage were administrated for 21 days, and arthritis was induced either in obese or non-obese rats via the injection with Freund’s complete adjuvant. Swelling of the paw was then measured. Plasma tumor necrosis factor (TNF-α), lipid profile, liver and kidney functions, serum lipid peroxidation, and erythrocyte catalase activity were determined.ResultsResults emphasized that arthritis with obesity resulted in the elevation of the swelling of the paw, TNF-α, dyslipidemia, and oxidative stress. Chia seeds oil and mucilage, more promisingly the oil, attenuated TNF-α and the swelling of the paw, improved lipid profile, and diminished the oxidative stress both in obese and non-obese arthritic rats.ConclusionsResults showed that chia seeds oil and mucilage exhibited anti-inflammatory effects against adjuvant-induced arthritis in obese and non-obese rats.

scholarly journals Dynamic Analysis of Metabolic Response in Gastric Ulcer (GU) Rats with Electroacupuncture Treatment Using 1H NMR-Based Metabolomics

2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Jia-cheng Shen ◽  
Lin-yu Lian ◽  
Yuan Zhang ◽  
Qi-da He ◽  
Jiao-long Chen ◽  
...  
Keyword(s):  
Gastric Ulcer ◽  
Metabolic Pathways ◽  
Metabolic Response ◽  
Complete Recovery ◽  
Pathological Examination ◽  
Good Effect ◽  
Intragastric Administration ◽  
Stomach Meridian ◽  
Liver And Kidney ◽  
Electroacupuncture Treatment

Gastric ulcer (GU), a common digestive disease, has a high incidence and seriously endangers health of human. According to the previous studies, it has been proved that electroacupuncture at acupoints of stomach meridian had a good effect on GU. However, there are few published studies on metabolic response in gastric ulcer (GU) rats with electroacupuncture treatment. Herein, we observed the metabolic profiles in biological samples (stomach, liver, and kidney) of GU rats with electroacupuncture treatment by 1H NMR metabolomics combined with pathological examination. The male SD rats were induced by intragastric administration of 70% ethanol after fasting for 24 hours and treated by electroacupuncture at Zusanli (ST36) and Liangmen (ST21) for 1 day, 4 days, or 7 days, respectively. And the conventional histopathological examinations as well as metabolic pathways assays were also performed. We found that GU rats were basically cured after electroacupuncture treatment for 4 days and had a complete recovery after electroacupuncture treatment for 7 days by being modulated comprehensive metabolic changes, involved in the function of neurotransmitters, energy metabolism, cells metabolism, antioxidation, tissue repairing, and other metabolic pathways. These findings may be helpful to facilitate the mechanism elucidating of electroacupuncture treatment on GU.

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