Faecalibacterium predicts postprandial glucose control following potatoes in overweight females
Abstract Background: Individual glycemic responses following dietary intake result from complex physiological processes and can be influenced by physical properties of foods, such as increased resistant starch (RS) from retrogradation of starch upon cooling after cooking. Predictive equations are needed to provide personalized recommendations for those individuals most at risk for poor metabolic outcomes. Methods: Thirty overweight women with no comorbid conditions participated in this randomized crossover trial, in which the women consumed 250g of hot (9.2 g RS) or cold (13.7 g RS) potatoes. Baseline characteristics included demographics, 10-day dietary records, body composition, and the relative abundance (RA) and α-diversity of gut microbiota. Elastic net regression using 5-fold cross-validation predicted postprandial glucose response (PPGR; incremental AUC0-120min) following the potatoes. Results: Thirty participants (29.6 ± 6.0 yrs; BMI 32.8 ± 3.7 kg/m2) participated in this trial. Most women (70%) showed a favorable PPGR to the cold potato. The model explained 32.2% of the variance in iAUC0-120min glucose with the equation: 547.65 x (0 [if cold potato], x 1 [if hot potato]) + (BMI[kg/m2] x 40.66) - (insoluble fiber[g] x 49.35) + (Bacteroides[RA] x 8.69) - (Faecalibacterium[RA] x 73.49) - (Parabacteroides[RA] x 42.08) + (α-diversity x 110.87) + 292.52.Conclusion: This model improves understanding of baseline characteristics that explain interpersonal variation in PPGR following potato intake and offers a tool to optimize dietary recommendations for a commonly consumed food. Larger studies are warranted to expand generalizability and application of the equation. Trial Registration: The National Clinical Trials number is NCT03310476, and this study was registered with clinicaltrials.gov on Oct 16, 2017.