scholarly journals Plasmon-Modulated Excitation-Dependent Fluorescence and Antimicrobial Activities of Silver and Gold Nanoparticles Prepared with Eugenia unifloraL. extracts

2021 ◽  
Author(s):  
Marcia Regina Franzolin ◽  
Daniella dos Santos Courrol ◽  
Karina de oliveira Gonçalves ◽  
Lilia Coronato Courrol
Keyword(s):  
Gold Nanoparticles ◽  
Field Enhancement ◽  
Ph Control ◽  
Antimicrobial Activities ◽  
Excitation Wavelength ◽  
Xenon Lamp ◽  
Local Field Enhancement ◽  
Silver And Gold Nanoparticles ◽  
Gram Positive Bacteria ◽  
Nanoparticles Stability

Abstract Green synthesis using plant extract is a sustainable method to obtain silver and gold nanoparticles (Ag and AuNPs) and was employed in this work. The Eugenia uniflora L. fruits and leaves extracts were used in nanoparticles synthesis. The photoreduction process with a xenon lamp and pH control improved optical properties and nanoparticles stability. The UV-vis, TEM, FTIR, and Zeta potential of the prepared solutions were obtained. The fluorescence spectra of Ag and AuNPs were investigated at different excitation wavelengths, which showed two kinds of fluorescence peaks. The shorter wavelength peaks red-shift with the increasing excitation wavelength, which results from the electron interband transitions, and the longer fixed wavelength peaks due to the local field enhancement. Finally, the antimicrobial tests were performed with Gram-negative and Gram-positive bacteria and Candida albicans. The best results were obtained with EuAgNPs prepared with fruits extract, photoreduction, and pH 7.0 (with a mean of 95.12% ± 10.29% of inhibition).

scholarly journals Size and Shape-Dependent Antimicrobial Activities of Silver and Gold Nanoparticles: A Model Study as Potential Fungicides

Molecules ◽  
2020 ◽  
Vol 25 (11) ◽  
pp. 2682 ◽  
Author(s):  
Francis J. Osonga ◽  
Ali Akgul ◽  
Idris Yazgan ◽  
Ayfer Akgul ◽  
Gaddi B. Eshun ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Pathogenic Fungi ◽  
Antimicrobial Activities ◽  
Plant Diseases ◽  
Production Plant ◽  
Silver And Gold Nanoparticles ◽  
Size And Shape ◽  
Model Study ◽  
Pathogenic Microbes ◽  
Bacteria And Fungi

Plant-based pathogenic microbes hinder the yield and quality of food production. Plant diseases have caused an increase in food costs due to crop destruction. There is a need to develop novel methods that can target and mitigate pathogenic microbes. This study focuses on investigating the effects of luteolin tetraphosphate derived silver nanoparticles (LTP-AgNPs) and gold nanoparticles (LTP-AuNPs) as a therapeutic agent on the growth and expression of plant-based bacteria and fungi. In this study, the silver and gold nanoparticles were synthesized at room temperature using luteolin tetraphosphate (LTP) as the reducing and capping agents. The synthesis of LTP-AgNPs and LTP-AuNP was characterized by Transmission Electron Microscopy (TEM) and size distribution. The TEM images of both LTP-AgNPs and LTP-AuNPs showed different sizes and shapes (spherical, quasi-spherical, and cuboidal). The antimicrobial test was conducted using fungi: Aspergillus nidulans, Trichaptum biforme, Penicillium italicum, Fusarium oxysporum, and Colletotrichum gloeosporioides, while the class of bacteria employed include Pseudomonas aeruginosa, Aeromonas hydrophila, Escherichia coli, and Citrobacter freundii as Gram (−) bacteria, and Listeria monocytogenes and Staphylococcus epidermidis as Gram (+) bacterium. The antifungal study demonstrated the selective size and shape-dependent capabilities in which smaller sized spherical (9 nm) and quasi-spherical (21 nm) AgNPs exhibited 100% inhibition of the tested fungi and bacteria. The LTP-AgNPs exhibited a higher antimicrobial activity than LTP-AuNPs. We have demonstrated that smaller sized AgNPs showed excellent inhibition of A. nidulans growth compared to the larger size nanoparticles. These results suggest that LTP-AuNP and LTP-AgNPs could be used to address the detection and remediation of pathogenic fungi, respectively.

scholarly journals Multi-Faceted Role of Silver and Gold Nanoparticles Synthesized from Biowaste and its in vitroAntibacterial, Antifungal and Antidiabetic Activities

2021 ◽  
Vol 11 (1) ◽  
pp. 3076-3092
Keyword(s):  
Gold Nanoparticles ◽  
Open Water ◽  
Antimicrobial Activities ◽  
Assay Method ◽  
Innovative Strategy ◽  
Silver And Gold Nanoparticles ◽  
Resazurin Assay ◽  
Ic50 Values ◽  
Uv Visible

Increasing global fish production, increasing the amount of waste generated, unsafe disposal of waste tissues like head, bones, skin, scales, fins etc., into land and open water reservoirs leads to environmental pollution. The role of nanobiotechnology in biowaste management is an innovative strategy to handle environmental issues. This study synthesized silver and gold nanoparticles from prawn heads using one millimolar AgNO3 and HAuCl4. Biosynthesized nanoparticles were characterized by UV -Visible spectroscopy, XRD, FTIR, SEM and EDAX. The maximum absorption spectrum was monitored at 437 nm for silver and 552 for gold nanoparticles. Antimicrobial activity was assessed using the resazurin assay method. MIC values obtained for the tested organisms revealed antimicrobial activities. P aeruginosa, K. pneumoniae, showed MIC at 15.6 µg for silver nanoparticles, and A. niger, A.flavus and C.albicans showed MIC at 125 µg for gold nanoparticles synthesized from the prawn head extract. IC 50 values of α- amylase activity were found to be 296 and 356 µg/ml for the silver and gold nanoparticles, respectively. IC50 values are about 705 and 2475 µg/ml for the silver and gold nanoparticles, respectively, in α-glucosidase activity. Conclusively silver and gold nanoparticles synthesized from prawn head extract (PHE) showed antibacterial, antifungal, and antidiabetic activities.

Antibacterial and Anti-Biofilm Biosynthesised Silver and Gold Nanoparticles for Medical Applications: Mechanism of Action, Toxicity and Current Status

2020 ◽  
Vol 17 (2) ◽  
pp. 88-100 ◽  
Author(s):  
Sundos Suleman Ismail Abdalla ◽  
Haliza Katas ◽  
Fazren Azmi ◽  
Mohd Fauzi Mh Busra
Keyword(s):  
Gold Nanoparticles ◽  
Synthesis Method ◽  
Chemical Characterization ◽  
Current Status ◽  
Silver And Gold Nanoparticles ◽  
Physical And Chemical Characterization ◽  
Biosynthesized Agnps ◽  
Physical And Chemical ◽  
Insight Into ◽  
Bio Synthesis

Fast progress in nanoscience and nanotechnology has contributed to the way in which people diagnose, combat, and overcome various diseases differently from the conventional methods. Metal nanoparticles, mainly silver and gold nanoparticles (AgNPs and AuNPs, respectively), are currently developed for many applications in the medical and pharmaceutical area including as antibacterial, antibiofilm as well as anti-leshmanial agents, drug delivery systems, diagnostics tools, as well as being included in personal care products and cosmetics. In this review, the preparation of AgNPs and AuNPs using different methods is discussed, particularly the green or bio- synthesis method as well as common methods used for their physical and chemical characterization. In addition, the mechanisms of the antimicrobial and anti-biofilm activity of AgNPs and AuNPs are discussed, along with the toxicity of both nanoparticles. The review will provide insight into the potential of biosynthesized AgNPs and AuNPs as antimicrobial nanomaterial agents for future use.

Stabilization of Silver and Gold Nanoparticles: Preservation and Improvement of Plasmonic Functionalities

2018 ◽  
Vol 119 (1) ◽  
pp. 664-699 ◽  
Author(s):  
Hyunho Kang ◽  
Joseph T. Buchman ◽  
Rebeca S. Rodriguez ◽  
Hattie L. Ring ◽  
Jiayi He ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Silver And Gold Nanoparticles

scholarly journals Plant antimicrobial peptides: structures, functions, and applications

2021 ◽  
Vol 62 (1) ◽  
Author(s):  
Junpeng Li ◽  
Shuping Hu ◽  
Wei Jian ◽  
Chengjian Xie ◽  
Xingyong Yang
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Peptides ◽  
Agricultural Production ◽  
Food Additives ◽  
Antimicrobial Activities ◽  
Gram Positive Bacteria ◽  
Potential Applications ◽  
Potential Applicability ◽  
Bacteria And Fungi ◽  
Positively Charged

AbstractAntimicrobial peptides (AMPs) are a class of short, usually positively charged polypeptides that exist in humans, animals, and plants. Considering the increasing number of drug-resistant pathogens, the antimicrobial activity of AMPs has attracted much attention. AMPs with broad-spectrum antimicrobial activity against many gram-positive bacteria, gram-negative bacteria, and fungi are an important defensive barrier against pathogens for many organisms. With continuing research, many other physiological functions of plant AMPs have been found in addition to their antimicrobial roles, such as regulating plant growth and development and treating many diseases with high efficacy. The potential applicability of plant AMPs in agricultural production, as food additives and disease treatments, has garnered much interest. This review focuses on the types of plant AMPs, their mechanisms of action, the parameters affecting the antimicrobial activities of AMPs, and their potential applications in agricultural production, the food industry, breeding industry, and medical field.

scholarly journals Isolation and Optimization of Culture Conditions of Thraustochytrium kinnei for Biomass Production, Nanoparticle Synthesis, Antioxidant and Antimicrobial Activities

2021 ◽  
Vol 9 (6) ◽  
pp. 678
Author(s):  
Kaliyamoorthy Kalidasan ◽  
Nabikhan Asmathunisha ◽  
Venugopal Gomathi ◽  
Laurent Dufossé ◽  
Kandasamy Kathiresan
Keyword(s):  
Antimicrobial Activity ◽  
Gold Nanoparticles ◽  
Silver Nanoparticles ◽  
Biomass Production ◽  
Petroleum Ether Extract ◽  
Antimicrobial Activities ◽  
Culture Conditions ◽  
Gold And Silver Nanoparticles ◽  
Optimization Of Culture Conditions ◽  
Antioxidant And Antimicrobial Activities

This work deals with the identification of a predominant thraustochytrid strain, the optimization of culture conditions, the synthesis of nanoparticles, and the evaluation of antioxidant and antimicrobial activities in biomass extracts and nanoparticles. Thraustochytrium kinnei was identified as a predominant strain from decomposing mangrove leaves, and its culture conditions were optimized for maximum biomass production of 13.53 g·L−1, with total lipids of 41.33% and DHA of 39.16% of total fatty acids. Furthermore, the strain was shown to synthesize gold and silver nanoparticles in the size ranges of 10–85 nm and 5–90 nm, respectively. Silver nanoparticles exhibited higher total antioxidant and DPPH activities than gold nanoparticles and methanol extract of the strain. The silver nanoparticles showed higher antimicrobial activity than gold nanoparticles and petroleum ether extract of the strain. Thus, Thraustochytrium kinnei is proven to be promising for synthesis of silver nanoparticles with high antioxidant and antimicrobial activity.

Interaction of Differently Sized, Shaped, and Functionalized Silver and Gold Nanoparticles with Glycosylated versus Nonglycosylated Transferrin

2021 ◽  
Author(s):  
Rinea Barbir ◽  
Rafael Ramírez Jiménez ◽  
Rafael Martín-Rapún ◽  
Vida Strasser ◽  
Darija Domazet Jurašin ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Silver And Gold Nanoparticles

scholarly journals Green Silver and Gold Nanoparticles: Biological Synthesis Approaches and Potentials for Biomedical Applications

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 844 ◽  
Author(s):  
Andrea Rónavári ◽  
Nóra Igaz ◽  
Dóra I. Adamecz ◽  
Bettina Szerencsés ◽  
Csaba Molnar ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Biomedical Applications ◽  
Biological Activities ◽  
Large Body ◽  
Chemical Properties ◽  
Biological Synthesis ◽  
Silver And Gold Nanoparticles ◽  
Comprehensive Collection ◽  
Synthetic Approaches ◽  
Physical And Chemical

The nanomaterial industry generates gigantic quantities of metal-based nanomaterials for various technological and biomedical applications; however, concomitantly, it places a massive burden on the environment by utilizing toxic chemicals for the production process and leaving hazardous waste materials behind. Moreover, the employed, often unpleasant chemicals can affect the biocompatibility of the generated particles and severely restrict their application possibilities. On these grounds, green synthetic approaches have emerged, offering eco-friendly, sustainable, nature-derived alternative production methods, thus attenuating the ecological footprint of the nanomaterial industry. In the last decade, a plethora of biological materials has been tested to probe their suitability for nanomaterial synthesis. Although most of these approaches were successful, a large body of evidence indicates that the green material or entity used for the production would substantially define the physical and chemical properties and as a consequence, the biological activities of the obtained nanomaterials. The present review provides a comprehensive collection of the most recent green methodologies, surveys the major nanoparticle characterization techniques and screens the effects triggered by the obtained nanomaterials in various living systems to give an impression on the biomedical potential of green synthesized silver and gold nanoparticles.

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