miR166h Directed Cleavage of Target Upon PGPR Inoculation Under Drought Stress and Tissue-Specific Expression Analysis Under Abiotic Stresses in Chickpea.

2021 ◽  
Author(s):  
Ankita Yadav ◽  
Sanoj Kumar ◽  
Rita Verma ◽  
Shashi Pandey Rai ◽  
Charu Lata ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Plant Growth ◽  
Stress Responses ◽  
Expression Patterns ◽  
Crop Improvement ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Growth Promoting

Abstract Legumes are an indispensable food after cereals with extensive production across the world. The legume production is imposed with limitations and has been augmented by various environmental stresses. The symbiotic relations between legumes and rhizobacteria have been an intriguing topic of research in view of their roles in plant growth, development and various stress responses. Recent advances on gene networks involving plethora of evolutionarily conserved miRNAs have been investigated pertaining to their roles in plant stress responses. The interaction between plant growth promoting rhizobacteria (PGPR) strain Pseudomonas putida RA, MTCC5279 and abiotic stress responsive miRNAs have previously been studied with roles in abiotic stress mitigation by modulating stress responsive miRNAs and their target genes. The present studyis an investigation involving the role of RA in abiotic stress responsive miR166h for drought mitigation in tolerant desi chickpea genotype. miRNA166 directed cleavage of its target, ATHB15 has been drifted of drought treated plantlets upon RA inoculation using 5´RLM-RACE analysis. Drought stressed chickpea plants when inoculated with growth promoting rhizobacteria, RA, the inverse correlation in expression patterns were noticed in miR166h and its validated target, ATHB15. Tissue-specific expression patterns in 15 days old chickpea seedlings including leaves, shoot and roots when exposed to salinity, drought and abscisic acid at different time points indicated the role of miR166 in different abiotic stress response. In view of the results, validation and functional characterization of such interactions involving stress responsive miRNAs along with microbial stress management techniques could be an important technique for crop improvement.

scholarly journals Arabidopsis NDL-AGB1 modules Play Role in Abiotic Stress and Hormonal Responses Along with Their Specific Functions

2019 ◽  
Vol 20 (19) ◽  
pp. 4736 ◽  
Author(s):  
Arpana Katiyar ◽  
Yashwanti Mudgil
Keyword(s):  
Gene Family ◽  
G Protein ◽  
Stress Responses ◽  
Abiotic Stresses ◽  
Molecular Mechanisms ◽  
Expression Patterns ◽  
Crop Improvement ◽  
Specific Expression ◽  
Hormonal Responses

Arabidopsis N-MYC Downregulated Like Proteins (NDLs) are interacting partners of G-Protein core components. Animal homologs of the gene family N-myc downstream regulated gene (NDRG) has been found to be induced during hypoxia, DNA damage, in presence of reducing agent, increased intracellular calcium level and in response to metal ions like nickel and cobalt, which indicates the involvement of the gene family during stress responses. Arabidopsis NDL gene family contains three homologs NDL1, NDL2 and NDL3 which share up to 75% identity at protein level. Previous studies on NDL proteins involved detailed characterization of the role of NDL1; roles of other two members were also established in root and shoot development using miRNA knockdown approach. Role of entire family in development has been established but specific functions of NDL2 and NDL3 if any are still unknown. Our in-silico analysis of NDLs promoters reveled that all three members share some common and some specific transcription factors (TFs) binding sites, hinting towards their common as well as specific functions. Based on promoter elements characteristics, present study was designed to carry out comparative analysis of the Arabidopsis NDL family during different stages of plant development, under various abiotic stresses and plant hormonal responses, in order to find out their specific and combined roles in plant growth and development. Developmental analysis using GUS fusion revealed specific localization/expression during different stages of development for all three family members. Stress analysis after treatment with various hormonal and abiotic stresses showed stress and tissue-specific differential expression patterns for all three NDL members. All three NDL members were collectively showed role in dehydration stress along with specific responses to various treatments. Their specific expression patterns were affected by presence of interacting partner the Arabidopsis heterotrimeric G-protein β subunit 1 (AGB1). The present study will improve our understanding of the possible molecular mechanisms of action of the independent NDL–AGB1 modules during stress and hormonal responses. These findings also suggest potential use of this knowledge for crop improvement.

Tissue-specific expression patterns of nuclear receptors

2013 ◽  
Author(s):  
AL Bookout ◽  
Y Jeong ◽  
M Downes ◽  
RT Yu ◽  
RM Evans ◽  
...  
Keyword(s):  
Nuclear Receptors ◽  
Expression Patterns ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression

scholarly journals Tissue Distribution of ACE2 Protein in Syrian Golden Hamster (Mesocricetus auratus) and Its Possible Implications in SARS-CoV-2 Related Studies

2021 ◽  
Vol 11 ◽  
Author(s):  
Voddu Suresh ◽  
Deepti Parida ◽  
Aliva P. Minz ◽  
Manisha Sethi ◽  
Bhabani S. Sahoo ◽  
...  
Keyword(s):  
Expression Pattern ◽  
Golden Hamster ◽  
Expression Patterns ◽  
Mesocricetus Auratus ◽  
Syrian Golden Hamster ◽  
Surface Epithelium ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Specific Expression Pattern

The Syrian golden hamster (Mesocricetus auratus) has recently been demonstrated as a clinically relevant animal model for SARS-CoV-2 infection. However, lack of knowledge about the tissue-specific expression pattern of various proteins in these animals and the unavailability of reagents like antibodies against this species hampers these models’ optimal use. The major objective of our current study was to analyze the tissue-specific expression pattern of angiotensin-converting enzyme 2, a proven functional receptor for SARS-CoV-2 in different organs of the hamster. Using two different antibodies (MA5-32307 and AF933), we have conducted immunoblotting, immunohistochemistry, and immunofluorescence analysis to evaluate the ACE2 expression in different tissues of the hamster. Further, at the mRNA level, the expression of Ace2 in tissues was evaluated through RT-qPCR analysis. Both the antibodies detected expression of ACE2 in kidney, small intestine, tongue, and liver. Epithelium of proximal tubules of kidney and surface epithelium of ileum expresses a very high amount of this protein. Surprisingly, analysis of stained tissue sections showed no detectable expression of ACE2 in the lung or tracheal epithelial cells. Similarly, all parts of the large intestine were negative for ACE2 expression. Analysis of tissues from different age groups and sex didn’t show any obvious difference in ACE2 expression pattern or level. Together, our findings corroborate some of the earlier reports related to ACE2 expression patterns in human tissues and contradict others. We believe that this study’s findings have provided evidence that demands further investigation to understand the predominant respiratory pathology of SARS-CoV-2 infection and disease.

The Murine Genome Contains Two Functional Kininogen Genes Leading to Tissue Specific Expression of High Molecular Weight Kininogen (HK) and Expression of a Novel HK Splice Variant.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 3979-3979
Author(s):  
Sergei Merkoulov ◽  
Anton A. Komar ◽  
Keith R. McCrae
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Expression Patterns ◽  
Endothelial Cell Proliferation ◽  
Head Orientation ◽  
High Molecular Weight Kininogen ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Exon 10

Abstract High molecular weight kininogen (HK) plays an important role in the assembly and activation of the kallikrein/kinin system. While the human genome contains only a single copy of the kininogen gene, three copies are present in the rat (one K-kininogen and two T-kininogen). Here, we report that the mouse genome contains two homologous kininogen genes (overall homology 91%), denoted mHK1 and mHK2. Both genes are located on chromosome 16 in a head-to-head orientation, and contain open reading frames. The size of intronic sequences between the 11 kininogen gene exons is similar (Figure). HK mRNA transcripts derived from the mHK1 and mHK2 genes differ slightly in size due to gaps of 33 and 18 nucleotides in exon 10 of mHK2. RT-PCR analysis of HK gene expression in adult and embryonic murine tissues revealed that HK mRNA was derived from mHK1 in liver, adrenal and embryo, but from mHK2 in kidney and lung. HK mRNA derived from both genes was present in testis, brain and muscle, though expression levels were low relative to those in other tissues. HK mRNA was not detected in ovary, bone marrow, heart or bladder. mHK1-derived HK mRNA was alternatively spliced, as demonstrated by the presence of an HK mRNA transcript encoding a novel HK1 isoform, ΔmD5, that lacked the portion of exon 10 encoding Thr400 - Asp582 of HK domains 5 and 6. Examination of the putative promoter regions of the two genes using the MatInspector Professional program (Genomatix) demonstrated distinct differences, perhaps explaining in part their tissue-specific expression patterns. Like domain 5 of human HK (hD5), domain 5 of murine HK (mD5), in which the histidine and lysine-rich C-terminal region of this domain previously shown to mediate the antiangiogenic activity of domain 5 is highly conserved, inhibited endothelial cell proliferation. While the function of each of the kininogen genes in the intact animal has yet to be defined, characterization of the two genes may provide new information concerning the role of high molecular weight kininogen in development, normal physiology, and pathological processes. Figure Figure

scholarly journals Tissue-Specific Expression Patterns of MicroRNA during Acute Graft-versus-Host Disease in the Rat

2016 ◽  
Vol 7 ◽  
Author(s):  
Dasaradha Jalapothu ◽  
Margherita Boieri ◽  
Rachel E. Crossland ◽  
Pranali Shah ◽  
Isha A. Butt ◽  
...  
Keyword(s):  
Graft Versus Host Disease ◽  
Expression Patterns ◽  
Specific Expression ◽  
Tissue Specific ◽  
Host Disease ◽  
Graft Versus Host ◽  
Tissue Specific Expression ◽  
Acute Graft

Tissue-specific expression of PPAR mRNAs in diabetic rats and divergent effects of cilostazol

2008 ◽  
Vol 86 (7) ◽  
pp. 465-471 ◽  
Author(s):  
Furong Wang ◽  
Ling Gao ◽  
Bendi Gong ◽  
Jianting Hu ◽  
Mei Li ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Renal Cortex ◽  
Expression Patterns ◽  
Diabetic Rats ◽  
Diabetic Patients ◽  
Peroxisome Proliferator ◽  
Specific Expression ◽  
Camp Content ◽  
Tissue Specific ◽  
Tissue Specific Expression

Cilostazol and ligands of peroxisome proliferator-activated receptors (PPARs) have been effectively used to alleviate diabetic complications, but the common and tissue-specific expression patterns of PPARs in different tissues in diabetic patients and those treated with cilostazol have not been reported. Here, we aimed to assess the effects of diabetes and cilostazol on mRNA expression of PPARα and PPARγ in the aorta, renal cortex, and retina of diabetic rats treated with cilostazol for 8 weeks. PPARα mRNA expression showed uniform downregulation in all these tissues in diabetic rats, and this effect was reversed by cilostazol treatment. Surprisingly, PPARγ mRNA expression was reduced in the renal cortex and retina, yet increased in the aorta of diabetic rats, although cilostazol still reversed these changes. Interestingly, cilostazol, a well-known phosphodiesterase 3 inhibitor and cAMP elevator, augmented cAMP content only in the aorta, but showed no significant effects in the renal cortex of diabetic rats. In conclusion, mRNA expression of PPARs is tissue-specific in diabetes and may be differently affected by cilostazol, possibly because of its tissue-specific effects on cAMP content.

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