scholarly journals Susceptibility of California Almond Cultivars to Aflatoxigenic Aspergillus flavus

HortScience ◽  
1994 ◽  
Vol 29 (1) ◽  
pp. 33-35 ◽  
Author(s):  
Thomas M. Gradziel ◽  
Dechun Wang
Keyword(s):  
Aspergillus Flavus ◽  
Prunus Dulcis ◽  
Fungal Colonization ◽  
Disease Development ◽  
Fungal Development ◽  
Intact Seed ◽  
Selection Of

Colonization and sporulation of aflatoxigenic Aspergillus flavus Link on intact and injured seed was evaluated for a selection of almond [Prunus dulcis (Mill.) D.A. Webb] cultivars. Barriers to fungal development were identified at the intact seedcoat and at the seed cotyledon tissue. The seedcoat barrier was expressed as a delay in fungal colonization for up to 3 days following the inoculation of intact seed. Seedcoat resistance was uniformly high for all cultivars tested. Cotyledon resistance, which was expressed as a lower rate of disease development was identified only in the cultivars Ne Plus Ultra, Ruby, and Carrion.

scholarly journals Selection of individual nutrition for patients with torpid course of atopic dermatitis

2020 ◽  
pp. 107-108
Author(s):  
Н.О. Артемьева ◽  
Е.В. Свечникова ◽  
Ю.В. Максимова ◽  
В.Н. Максимов
Keyword(s):  
Quality Of Life ◽  
Atopic Dermatitis ◽  
Inflammatory Disease ◽  
Skin Diseases ◽  
Disease Development ◽  
Physical Discomfort ◽  
Selection Of

Атопический дерматит (АД) - это воспалительное заболевание кожи, мультифакториальной или моногенный природы, характеризующееся зудом, хроническим рецидивирующим течением и возрастными особенностями локализации и морфологии очагов поражения. Доля заболевания в структуре кожных болезней в настоящее время составляет от 10 до 30%. Когда процесс приобретает упорное течение с невыраженным эффектом от проводимой терапии и обострениями процесса до 3-4 в год с увеличением их длительности, то становится вялотекущим (торпидным). Торпидное течение АД приводит больного к снижению качества жизни за счёт физического дискомфорта и эмоциональной дезадаптации. Подходы к лечению в теории зависят от механизма развития заболевания. Atopic dermatitis (AD) is an inherited inflammatory disease of skin, multifactor or monogenic nature, characterized by itching, chronic recurrent current and age-specific features of localisation and morphology of lesion centers. The proportion of the disease in the structure of skin diseases is currently between 10 and 30%. When the process acquires a persistent course with an unparalleled effect from the performed therapy and exacerbations of the process up to 3-4 per year with increase of their duration, it becomes sluggish (torpedo) [1, 3]. The torpedo course of AD leads the patient to a decrease in the quality of life due to physical discomfort and emotional disadaptation. Approaches to treatment in theory depend on the mechanism of disease development.

Susceptibility of almond (Prunus dulcis) cultivars to twig canker and shoot blight caused by Diaporthe amygdali

Plant Disease ◽  
2022 ◽  
Author(s):  
Francisco Beluzán ◽  
Xavier Miarnau ◽  
Laura Torguet ◽  
Lourdes Zazurca ◽  
Paloma Abad-Campos ◽  
...  
Keyword(s):  
Agronomic Traits ◽  
Field Tests ◽  
Prunus Dulcis ◽  
Breeding Programs ◽  
Relevant Variables ◽  
Shoot Blight ◽  
Blight Disease ◽  
Cultivar Susceptibility ◽  
The Relationship ◽  
Selection Of

Twenty-five almond cultivars were assessed for susceptibility to Diaporthe amygdali, causal agent of twig canker and shoot blight disease. In laboratory experiments, growing twigs were inoculated with four D. amygdali isolates. Moreover, growing shoots of almond cultivars grafted onto INRA ‘GF-677’ rootstock were used in four-year field inoculations with one D. amygdali isolate. In both type of experiments, inoculum consisted of agar plugs with mycelium, which were inserted underneath the bark and the lesion lengths caused by the fungus were measured. Necrotic lesions were observed in the inoculated almond cultivars both in laboratory and field tests, confirming the susceptibility of all the evaluated cultivars to all the inoculated isolates of D. amygdali. Cultivars were grouped as susceptible or very susceptible according to a cluster analysis. The relationship between some agronomic traits and cultivar susceptibility was also investigated. Blooming and ripening times were found relevant variables to explain cultivars performance related to D. amygdali susceptibility. Late and very late blooming, and early and medium ripening cultivars were highly susceptible to D. amygdali. Our results may provide valuable information that could assist in ongoing breeding programs of this crop and additionally in the selection of cultivars for new almond plantations.

Cyanogenic potential of Trifolium repens L. in relation to pepper spot caused by Stemphylium sarciniforme

1978 ◽  
Vol 56 (20) ◽  
pp. 2491-2496 ◽  
Author(s):  
H. T. Wilkinson ◽  
R. L. Millar
Keyword(s):  
White Clover ◽  
Trifolium Repens ◽  
Fungal Colonization ◽  
Symptom Expression ◽  
Symptom Development ◽  
Cyanogenic Glucosides ◽  
Fungal Development ◽  
Cyanogenic Potential ◽  
Trifolium Repens L ◽  
Whole Mounts

White clover (Trifolium repens L.) contains two cyanogenic β-glucosides (G), linamarin and lotaustralin. Upon injury to the tissues a β-glucosidase (E) effects the release of cyanide from the β-glucosides. Four selections of white clover differing in cyanogenic properties (G+E+, G+E−, G−E+, G−E−) were examined for differences in susceptibility to Stemphylium sarciniforme. Symptom development was determined at 24, 48, 72, 96, and 120 h after the leaves were inoculated. Symptoms for all four selections consisted initially of small brown flecks (1 mm in diameter), then dark spots (1–2 mm in diameter) with definite borders, and finally senescence and wilt of the interlesion tissue. Plants containing both β-glucosidase (EC 3.2.1.21)and cyanogenic glucosides (G+E+) and those with only glucosides (G+E−) developed symptoms 24 h earlier than did the G−E+ and G−E− selections. The earliest symptoms were observed at 48 h; by 96 h all four selections showed comparable degrees of senescence and wilt. Pathogen development in infected leaves prepared as whole mounts was examined microscopically. Through 72 h after inoculation, development of S. sarciniforme in the G+E+ and G+E− selections was slower than in the G−E+ selections. By 96 h, however, fungal development in all four selections was comparable; commencement of leaf senescence coincided with extensive fungal colonization of necrotic tissue. Accelerated rates of symptom expression and retarded pathogen development apparently were positively correlated with cyanogenic potential of each white clover selection. Necrosis of suscept tissue was not correlated with cyanogenic potential of each selection.

FUNGAL COLONIZATION AND ZEARALENONE ACCUMULATION IN MAIZE EARS INJURED BY BIRDS

1980 ◽  
Vol 60 (2) ◽  
pp. 453-461 ◽  
Author(s):  
J.C. SUTTON ◽  
W. BALIKO ◽  
H.J. LIU
Keyword(s):  
Zea Mays ◽  
Fusarium Moniliforme ◽  
Crude Protein ◽  
Dry Matter ◽  
Compensatory Growth ◽  
Zea Mays L ◽  
Fungal Growth ◽  
Agelaius Phoeniceus ◽  
Fungal Colonization ◽  
Fungal Development

Relationships of depredation by red-winged blackbirds (Agelaius phoeniceus L.) and simulated bird injury to fungal development, zearalenone accumulation, yield and protein in maize (Zea mays L.) ears were examined. Natural and simulated bird injury were inflicted when the kernels were at the milk or soft-dough stages of development. Both treatments predisposed kernels to fungal colonization. In noninjured maize, fungi proliferated on few kernels of only a few ears. However, in injured maize, injured kernels were colonized rapidly by weakly aggressive fungi and noninjured kernels were colonized frequently and often extensively by Fusarium moniliforme Sheldon and Fusarium graminearum Schwabe. Loosening and shredding of husks was a factor in predisposition to fungal growth. The mycotoxin zearalenone was found (0.5–7.0 μg/g dried kernels) in ears injured by birds or artificially, but not in noninjured ears. Yields of dried kernels in injured ears generally declined with increased natural or simulated bird injury, but often there was compensatory growth of noninjured kernels in injured ears. Crude protein usually constituted a higher percentage of kernel dry matter in injured ears than in noninjured ears.

scholarly journals ISOLATION AND SELECTION OF AFLATOXINS PRODUCING ASPERGILLUS FLAVUS FROM PEANUT

2018 ◽  
Vol 55 (5A) ◽  
pp. 125
Author(s):  
Le Thi Phuong Thao
Keyword(s):  
Aspergillus Flavus ◽  
Animal Health ◽  
Initial Step ◽  
Isolation And Identification ◽  
Contamination Control ◽  
Its Gene ◽  
Pcr Products ◽  
Main Producer ◽  
United Nation ◽  
Selection Of

Aspergillus flavus is known as the main producer of carcinogenic aflatoxins. The presence of this fungus and aflatoxins is an important impactson food safety, human and animal health. Peanut is one of the most important crops in Vietnam and  is suitable to substrate for aflatoxins producing fungus growth. The isolation and identification of Aspergillus flavus species from peanut is an initial step for further study in Aflatoxins contamination control in peanut. 28 peanut samples selected in Luc Nam district - Bac Giang province were used for fungus isolation. The isolated fungus were purified, conducted DNA extract, amplified PCR products then performed sequencing ITS gene and used BLAST software to check alignment to identification the strains. As the result, 4 strains alignedwith Aspergillus flavus strains from National Center for Biology Information - United Nation such as: TUHT115, KP214054.1, MTCC 8654, ZJ4-A. Those strains were checked and confirmed the aflatoxins production in peanut samples.

A survey ofPenicillium brevicompactumandP. bialowiezensefrom indoor environments, with commentary on the taxonomy of theP. brevicompactumgroupThis paper is one of a selection of papers published in the Special Issue on Systematics Research.

Botany ◽  
2008 ◽  
Vol 86 (7) ◽  
pp. 732-741 ◽  
Author(s):  
James A. Scott ◽  
Bess Wong ◽  
Richard C. Summerbell ◽  
Wendy A. Untereiner
Keyword(s):  
Internal Transcribed Spacer ◽  
Pcr Amplification ◽  
Fungal Colonization ◽  
Indoor Environments ◽  
Special Issue ◽  
Reproduction Mode ◽  
Penicillium Brevicompactum ◽  
Sample Set ◽  
Selection Of ◽  
Β Tubulin

We investigated the diversity of the Penicillium brevicompactum Dierckx group in dust from 54 houses in Wallaceburg, Ontario, Canada. Two taxa were predominant, P. brevicompactum and Pencillium bialowiezense Zaleski, accounting for 88.6% and 5.4% of the sample set, respectively. We further characterized multilocus haplotypes of isolates by characterizing three polymorphic genetic loci, β-tubulin (benA), histone 4 (his4A), and the internal transcribed spacer regions of ribosomal DNA (nucITS) amplified by PCR amplification and screened using heteroduplex mobility assay (HMA). Eight unique haplotypes were observed in P. brevicompactum s. str., and two in P. bialowiezense, both with a distribution characteristic of a predominantly clonal reproduction mode. Phylogenetic analysis of the β-tubulin and nucITS loci were carried out for members of the P. brevicompactum group, including ex-type material, that revealed three well-supported lineages corresponding to P. brevicompactum, P. bialowiezense (=Penicillium biourgeianum Zaleski), and Penicillium neocrassum R. Serra & S.W. Peterson. The mycophilic nature of many isolates of P. bialowiezense, and some isolates of P. brevicompactum, suggests that observation of members of the P. brevicompactum group in indoor environments may predict extensive and longterm fungal colonization. We also address some nomenclatural problems in the group and epitypify P. bialowiezense.

scholarly journals The PHD Transcription Factor Cti6 is Involved in the Fungal Colonization and Aflatioxin B1 Biological Synthesis of Aspergillus Flavus

2020 ◽  
Author(s):  
Mengjuan Zhang ◽  
Guanglan Lin ◽  
Xiaohua Pan ◽  
Weitao Song ◽  
Can Tan ◽  
...  
Keyword(s):  
Aspergillus Flavus ◽  
Biological Function ◽  
Vital Role ◽  
Biological Synthesis ◽  
Pcr Analysis ◽  
Fungal Colonization ◽  
Mycelium Growth ◽  
Formation Pathway ◽  
Novel Target ◽  
Phd Domain

Abstract Aspergillus flavus and its main secondary metabolite AFB1 pose a serious threat to several important crops worldwide. Recently, it has been reported that some PHD family transcription factors are involved in the morphogenesis and AFB1 biological synthesis in A. flavus, but the role of Cti6, a PHD domain containing protein in A. flavus, is totally unknown. The study was designed to reveal the biological function of Cti6 in the fungus by deletion of cti6, and its two domains (PHD and Atrophin-1) through homologous recombination, respectively. The results showed that Cti6 might up-regulate the mycelium growth, conidiation, sclerotia formation and AFB1 biological synthesis of A. flavus by its PHD domain, while Atrophin-1 also improved the conidiation of the fungus. The qRT-PCR analysis showed that Cti6 increased the conidiation of the fungus through AbaA and BrlA mediated conidiation pathway, triggered the formation of sclerotia by orthodox sclerotia formation pathway, and improved the production of AFB1 by orthodox AFB1 synthesis pathway. Crops models analysis showed that A. flavus Cti6 plays vital role in colonization and the production of AFB1 on the host grains mainly via PHD domain. Bioinformatics analysis showed Cti6 is conservative in Aspergillus spp., and mCherry mediated subcellular localization showed that most Cti6 accumulated in the nuclei, which reflected that Cti6 performed its important biological function in the nuclei in Aspergillus spp.. The results of the current study elucidate the roles of PHD domain containing proteins in the mechanism of the infection of crops by A. flavus, and provided a novel target for effectively controlling the contamination of Aspergillus spp. to crops.

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