scholarly journals In Vitro Root Formation of Derooted Jujube Seedlings Induced by Agrobacterium rhizogenes

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 888E-888
Author(s):  
Muhammad Hatta ◽  
Caula A. Beyl ◽  
Stephen Garton
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Root Length ◽  
Root Formation ◽  
Great Difficulty ◽  
Differential Response ◽  
Root Number ◽  
Ziziphus Jujuba ◽  
Positive Effect ◽  
Roots In Vitro

Jujube (Ziziphus jujuba) is a plant whose cuttings root with great difficulty. Several strains of Agrobacterium rhizogenes were tested on two segments (proximal and basal) of derooted jujube seedlings in the presence or absence of IBA for induction of roots in vitro. Strains of A. rhizogenes used were A4, A4pARC8, TR105, and R1000. Strain, segment of derooted seedling, and IBA influenced inoculation success. Strains A4pARC8 and TR105 were more infective than A4 and R1000. Basal segments were more responsive than proximal ones and 0.5 mg·liter–1 1BA had a positive effect on inoculation success. Agrobacterium rhizogenes and IBA were very effective in promoting root formation, root number, root length, and early root emergence. They worked synergistically, but were effective independently as well. A4pARC8 and TR105 were more effective than strains A4 and R1000, and generally more effective than IBA. lBA was unable to eliminate the differential response of the two segments for individual strains.

scholarly journals Induction of Roots on Jujube Softwood Cuttings Using Agrobacterium rhizogenes

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 888D-888
Author(s):  
Muhammad Hatta ◽  
Caula A. Beyl ◽  
Stephen Garton
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Tree Species ◽  
Root Length ◽  
Great Difficulty ◽  
Fruit Tree ◽  
Greenhouse Experiment ◽  
Root Number ◽  
Flower Buds ◽  
Ziziphus Jujuba ◽  
Woody Ornamental

Trees of jujube (Ziziphus jujuba), particularly older ones, root with great difficulty. A greenhouse experiment was conducted to assess the effects of two strains of Agrobacterium rhizogenes (A4 and TR105) on softwood cuttings from two trees—a tree 10 years old not currently bearing flowers, which we called “juvenile” because it still exhibited many juvenile characteristics; and a tree ≈70 years old containing many flower buds, which we called “mature”. The cuttings were collected on 11 May 1994 and trimmed to 7.5 cm. Both strain and source of cutting influenced inoculation success—TR105 was more responsive to A. rhizogenes than was A4 and the “juvenile” cuttings more responsive than “mature” cuttings. Strain TR105 was very effective in increasing rooting percentages and root number. “Juvenile” cuttings had better rooting percentages, greater root number, and greater root length than did “mature” cuttings. Agrobacterium rhizogenes exhibits great potential for rooting other difficult woody ornamental or fruit tree species as well.

scholarly journals In vitro root formation and plantlet development in dendrobium orchid

1970 ◽  
Vol 35 (2) ◽  
pp. 257-265 ◽  
Author(s):  
MM Khatun ◽  
H Khatun ◽  
D Khanam ◽  
Md Al-Amin
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Root Length ◽  
Root Formation ◽  
Combined Effect ◽  
In Vitro Rooting

The experiment was conducted to investigate the combined effect of different plant growth regulators with and without charcoal supplementation for root formation and plantlet development from protocorm like bodies (PLBS) of orchid. The combination of BAP + NAA, BAP + IAA, BAP + IBA, and IAA + IBA at different concentrations were studied. It revealed that the highest number of roots was obtained from 1.0 mg/L each of IAA + IBA combination (6.667) and the highest root length was recorded from 2.0 mg/L BAP + 1.0 mg/L IBA with charcoal supplementation. The treatment combinations, 1.0 mg/L each of BAP + NAA, BAP + IAA, BAP + IBA, and IAA + IBA were found best for producing more rooted plantlets with charcoal supplementation. It revealed that charcoal enhanced the root formation. Keywords: Orchid; Dendrobium; hybrid; In vitro rooting. DOI: 10.3329/bjar.v35i2.5888Bangladesh J. Agril. Res. 35(2) : 257-265, June 2010

scholarly journals Estimation of the efficiency of rooting varieties of Berberis Thunbergia DC. by the method of green shearing

2021 ◽  
Vol 5 (47) ◽  
pp. 22-22
Author(s):  
Maxim Simakhin ◽  
Yulia Dotsenko ◽  
Tatiana Aniskina ◽  
Vitaly Donskikh ◽  
Inna Zubik ◽  
...  
Keyword(s):  
Analysis Of Variance ◽  
Butyric Acid ◽  
Root Length ◽  
Root Formation ◽  
Total Root Length ◽  
Berberis Thunbergii ◽  
Total Length ◽  
Rooting Of Cuttings ◽  
Positive Effect ◽  
Three Factor

In this work, the results of evaluating the differences in the variants of the three-factor experiment on rooting of varieties of Barberry Thunberg (Berberis thunbergii DC.) By factors: varietal characteristics, the number of nodes and the use of a root formation regulator are considered. The study involved cuttings of the following varieties: ‘Dart’s Red Lädy’, ‘Kobold’, ‘Golden Ring’, ‘Harlequin’, ‘Rosetta’, ‘Red Chief’ and ‘Aurea’. 4- (indol-3-yl) butyric acid (trademark "Kornevin") was used as a root formation stimulator by the method of dusting. The green cuttings had 2 and 3 knots. The results of analysis of variance on the variability of the average total root length in green cuttings depending on varietal characteristics, dusting with a root formation stimulator and the number of nodes made it possible to establish that the ‘Red Chief’ variety had the maximum average total root length and was significantly different from all other varieties. The average total length of roots when using a root formation stimulator is significantly higher than in the control variant. Cuttings with three nodes reliably had a large average total root length. Based on the results obtained, it is assumed that the use of a root formation stimulator and rooting of cuttings with three nodes has a positive effect on the rooting processes of barberry cuttings. Keywords: BERBERIS THUNBERGII, THUNBERG BARBERRY, GREEN CUTTINGS, 4- (INDOL-3-YL) BUTYRIC ACID, KORNEVIN, KNOTS

scholarly journals ВМІСТ ФОТОСИНТЕТИЧНИХ ПІГМЕНТІВ У РОСЛИНАХ РОДУ CARLINA L. У ПРИРОДІ ТА КУЛЬТУРІ IN VITRO

2020 ◽  
Vol 78 (4) ◽  
pp. 16-23
Author(s):  
N. B. Kravets ◽  
L. R. Hrytsak ◽  
M. Z. Prokopyak ◽  
O. Yu. Mayorova ◽  
N. M. Drobyk
Keyword(s):  
Acid Solution ◽  
Growth Regulators ◽  
Root Formation ◽  
Cultivated Plants ◽  
Presowing Treatment ◽  
Murashige Skoog ◽  
Half Strength ◽  
Semi Solid ◽  
Positive Effect

im. The aim of the study was to choose conditions for rooting improvement of in vitro cultivated plants of some species of Carlina L. genus. Methods. For receiving and rooting of aseptic sprouts, seeds of Carlina acaulis L., Carlina cirsioides Klok and Carlina onopordifolia Besser ex Szafer, Kulcz. et Pawl were subjected to presowing treatment with gibberellic acid solution (GA3) or indolebyturic acid solution (IBA). Sterilized seeds were planted in sterile Petri dishes on semi-solid Murashige, Skoog nutrient medium with half-strength concentrations of macro- and microsalts without growth regulators. Results. It was found that with the seed soaking of C. acaulis, C. cirsioides and C. onopordifolia in GA3 solution the percentage of root formation amounted to 33.3 %, 33.3 % and 22.2 % respectively. Presowing treatment of carlina seeds in IBA solution with concentration of 1000 mg for 2–4 hours before sterilization gave a positive effect: the percentage of root formation for C. acaulis, C. cirsioides and C. onorordifolia was 2.4–4.5 times higher compared to the treatment with GA3 solution. Conclusions. To form the root system of carlina plants it is effective to soak the seeds in the solution of IBA. Thus we were able to increase the percentage of rooting of C. sirsioides and C. onorordifolia plants to 100 %, C. acaulis plants – up to 80 % and avoid sprouts’ injury and changes in the concentrations of the IBA, which may occur during sterilization at high temperatures by using non-sterile solution of growth regulators.

scholarly journals The influence of Cinnamomum zeylanicum in plant tissue culture experiments of Gardenia jasmenoides Ellis

2013 ◽  
Vol 10 (4) ◽  
pp. 1102-1107
Author(s):  
Baghdad Science Journal
Keyword(s):  
Root Formation ◽  
Culture Media ◽  
Shoot Proliferation ◽  
Naphthalene Acetic Acid ◽  
Alcoholic Extract ◽  
Fresh Weight ◽  
Benzyl Adenine ◽  
Cinnamomum Zeylanicum ◽  
Positive Effect

Cinnamon plant is considered one of important medicinal plants because it is rich with many active compounds. This research is aimed to study possible effects of extract in culture media of Gardenia jasmenoides. Alcoholic extract was prepared from the bark of cinnamon at different concentrations (0.0, 1.0, 2.0) mg/L, then added to culture media to notice the effect of these concentrations on the growth and development of tissues and organs of Gardenia jasmenoides Ellis in vitro. Results showed the positive effect of increasing callus fresh weight and shoot proliferation from single nodes with presence of plant regulators, 5.0 mg/L Naphthalene acetic acid (NAA) and 3.0 mg/L Benzyl adenine (BA). Results showed that extract has a slight effect on root formation with the presence of plant regulators or when it is alone.

scholarly journals In vitro CULTURE OF BIG-SAGE (Lantana camara L.) PLANT

2020 ◽  
Vol 19 (2) ◽  
pp. 67-73
Author(s):  
Majid Abdulhameed Ibrahim ◽  
Manal Zebari Sabty ◽  
Shaimaa Hussein Mussa
Keyword(s):  
Root Length ◽  
Root Formation ◽  
Callus Formation ◽  
Shoot Multiplication ◽  
Lantana Camara ◽  
The Other ◽  
Ms Medium ◽  
Formation Number ◽  
Number Of Shoots

The study was conducted to mass micropropagation of big sage (Lantana camara L.) plant by shoot multiplication technique. The treatments 2.22 and 2.66 µmol·L–1 BA gave the highest significant increase in the percentage of response to shoot multiplication and number of shoots per explant compared to the other treatments as reached 96.70% and 100.00% and 4.33 and 6.00 shoots, respectively. The results showed that these two treatments did not differ significantly between them. While the 1.33 µmol·L–1 BA gave the lowest values in the percentage of response to shoot multiplication and number of shoots per explant were 80.00% and 2.00 shoots per explant, respectively. The MS medium supplemented with 4.30 or 5.37 µmol·L–1 NAA gave a high response to root formation, number of roots per shoot and root length. While the MS medium supplemented with 6.44 or 7.52 µmol·L–1 NAA gave low values in these characteristics. The MS medium with 2.22 or 2.66 µmol·L–1 concentration of BA or 7.52 µmol·L–1 concentration of NAA recorded the highest significant increase in the percentage of response to callus formation. While the MS medium supplemented with 1.33 µmol·L–1 BA or 4.30 µmol·L–1 NAA gave less response to the callus formation.

scholarly journals In vitro rooting of promising Lonicera caeruleae L. cultivars

2021 ◽  
Vol 4 (46) ◽  
pp. 20-20
Author(s):  
Alexander Saakian ◽  
◽  
Keyword(s):  
Nutrient Medium ◽  
Root Formation ◽  
Indoleacetic Acid ◽  
In Vitro Rooting ◽  
Lonicera Caerulea ◽  
Iron Chelate ◽  
Different Types ◽  
Positive Effect

Abstract The work is devoted to the improvement of the in vitro rooting technique of valuable Lonicera caerulea L. cultivars. The influence of different types of auxins and iron sources was determined at the stage of rhizogenesis. The use of indolylbutyric acid at a concentration of 1.0 mg/l was effective for the rooting of the cultivars Solovey and Yugana: the rooting rate was 60% and 99%, respectively. When using indoleacetic and indolylbutyric acids no significant differences were found in the percentage of rooting rates in the cultivars Gzhelka, Dlinnoplodnaya, Knyaginya and Lenya. It was found that 200 mg/l of iron chelate (Fe(III)-EDDHA) and 0.5 mg/l of indoleacetic acid in the nutrient medium had a positive effect on the dynamics of root formation of most honeysuckle cultivars. It was preferable to use a nutrient medium with the addition of iron chelate Fe(III)-EDTA at a concentration of 73.4 mg / l for the cultivar Yugana (rooting rate was 76%). Keywords: LONICERA CAERULEA, CULTIVARS, IN VITRO, RHIZOGENESIS, AUXINS, SOURCES OF IRON

scholarly journals The Effect of MS strength, pH and Sucrose Concentrations on In vitro Propagation of Okra (A belmoschus esculentus L.) from Shoot Tip Explants .

2021 ◽  
Author(s):  
Girma Gebrehiwot Woldeyes ◽  
Tileye Feyissa Senbeta ◽  
Alelegne Yeshamebel Adugna ◽  
Agegnehu Wasse Abegaz
Keyword(s):  
Activated Charcoal ◽  
Root Length ◽  
Sucrose Concentration ◽  
Shoot Multiplication ◽  
Shoot Tips ◽  
Leaf Number ◽  
Root Number ◽  
Inhibiting Effect ◽  
Ph Level

Abstract BackgroundOkra (Abelmoschus esculentus) is belongs to the family Malvaceae and genus Hibiscus. Conventional propagation of okra in a large scale is limited due to bacterial, fungal, viral disease and seed dormancy. Hence, micropropagation offers great potential to increasing the commercial availability of okra. This study aimed to investigate the effect of MS strength, sucrose concentration, and pH on the in vitro propagation of A. esculentus from shoot tips. ResultsOkra seeds were sterilized with 70% alcohol, 30% (v/v) Berekina and 0.13% HgCl2 (w/v) for 5, 40 and 10 minutes respectively. The shoots were excised from in vitro germinated seedlings and transferred to full strength MS liquid medium containing 1mg/L BAP. The in vitro initiated shoot tips were transferred to different MS strength (1, ½, ⅓, ¼) medium, sucrose concentration (30 g/L, 20 g/L, 15 g/L, 10 g/L) and pH level (pH 6.6, 6.2, 5.8, 5.4, 5.0) that contained 1.5 mg/L BAP and 200 mg/L activated charcoal. For rooting, different MS strength (full, ½, ⅓, ¼), sucrose (30 g/L, 20 g/L, 15 g/L and 10 g/L) and pH (6.6, 6.2, 5.8, 5.4, 5.0) that contained 1mg/L IBA with 200 mg/L activated charcoal were used. Full strength MS medium containing 30 g/L sucrose at pH 5.8 produced the highest mean shoot number (7.93), mean leaf number (14.37) and mean length (6.4 cm) per explant. Similarly, at pH 6.2 and 6.6 maximum shoot mean number (6.53, 5.80), leaf number (12.77, 10.43) and shoot length (6.32, 5.82 cm) were produced respectively. Maximum mean root number (15.73) and root length (6.05 cm) were recorded at ½ MS strength. At pH 5.8, 6.2 and 6.6 maximum mean root number (1.77, 1.47, 1.80) and root length (1.38, 1.25, 1.39 cm) were obtained. After acclimatization, 86% of plants survived in greenhouse. ConclusionDecreasing the MS strength, sucrose concentration and pH level had an inhibiting effect on both shoot multiplication and root formation of okra. Besides increasing pH value showed inhibiting effect on shoot multiplication.

scholarly journals Germinação in vitro e aclimatização de Hamatocactus setispinus

2015 ◽  
Vol 21 (2) ◽  
pp. 193
Author(s):  
Priscilla Brites Xavier ◽  
Janie Mendes Jasmim
Keyword(s):  
Root Length ◽  
Dry Matter ◽  
Seedling Survival ◽  
Basal Medium ◽  
Shoot Length ◽  
In Vitro Germination ◽  
Root Number ◽  
Fresh Matter ◽  
Dry Matter Weight

Seed propagation preserves the population genetic variability and helps selecting desirable features. This study evaluated the in vitro germination of Hamatocactus setispinus in six different culture media, 1- MS basal medium full strength; 2- half-strength MS basal medium; 3- 1.0 g L-1 of Peter’s CalMag® 15-05-15 formulation; 4- 0.5 g L-1 of Peter’s CalMag® 15-05-15 formulation; 5- MS basal medium supplemented with 10% coconut water and; 6- water and agar, with and without activated charcoal, and the speed of germination index, the mean germination time and the germination rate, root length, shoot length and the number of roots were evaluate. The seedlings with superior development obtained from in vitro germination were acclimatized in two substrates: Biomix® Floreira; Biomix® Floreira + sand. Seedling survival, shoot length, shoot diameter, root length, root number, shoot fresh matter weight, root fresh matter weight, shoot dry matter weight and root dry matter weight were evaluated. Peter’s 1.0 g L-1 medium without activated charcoal led to the best results for root length (11.36 mm) and root number (3.84). There was 100% of seedling survival. Acclimatization substrates did not differ among themselves and, therefore, they did not affect seedling growth.

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