scholarly journals In vitro rooting of promising Lonicera caeruleae L. cultivars

2021 ◽  
Vol 4 (46) ◽  
pp. 20-20
Author(s):  
Alexander Saakian ◽  
◽  
Keyword(s):  
Nutrient Medium ◽  
Root Formation ◽  
Indoleacetic Acid ◽  
In Vitro Rooting ◽  
Lonicera Caerulea ◽  
Iron Chelate ◽  
Different Types ◽  
Positive Effect

Abstract The work is devoted to the improvement of the in vitro rooting technique of valuable Lonicera caerulea L. cultivars. The influence of different types of auxins and iron sources was determined at the stage of rhizogenesis. The use of indolylbutyric acid at a concentration of 1.0 mg/l was effective for the rooting of the cultivars Solovey and Yugana: the rooting rate was 60% and 99%, respectively. When using indoleacetic and indolylbutyric acids no significant differences were found in the percentage of rooting rates in the cultivars Gzhelka, Dlinnoplodnaya, Knyaginya and Lenya. It was found that 200 mg/l of iron chelate (Fe(III)-EDDHA) and 0.5 mg/l of indoleacetic acid in the nutrient medium had a positive effect on the dynamics of root formation of most honeysuckle cultivars. It was preferable to use a nutrient medium with the addition of iron chelate Fe(III)-EDTA at a concentration of 73.4 mg / l for the cultivar Yugana (rooting rate was 76%). Keywords: LONICERA CAERULEA, CULTIVARS, IN VITRO, RHIZOGENESIS, AUXINS, SOURCES OF IRON

scholarly journals Improvement of clonal micropropagation technique of promising Lonicera caerulea L. cultivars

2021 ◽  
Vol 941 (1) ◽  
pp. 012030
Author(s):  
N D Orlova ◽  
O I Molkanova ◽  
O V Koroleva
Keyword(s):  
Nutrient Medium ◽  
In Vitro Propagation ◽  
Lonicera Caerulea ◽  
Clonal Micropropagation ◽  
Medium Components ◽  
Second Year ◽  
Propagation Technique ◽  
The Impact ◽  
Positive Effect

Abstract The work is focused on enhancement of in vitro propagation technique of valuable Lonicera caerulea L. cultivars. The impact of some nutrient medium components at main cultivation stages of the genus Lonicera L. representatives was studied. Some cultivars showed the positive effect of substituting sucrose for glucose in the medium at the multiplication stage (Diana (30 or 40 g L−1), Moskovskaya 23 (20 g L−1) and Yugana (40 g L−1)). When studying the influence of different auxin types on honeysuckle rhizogenesis, Solovey and Yugana preferred addition of 1.0 mg L−1 IBA into the medium. During the research of influence of different auxin concentrations on L. caerulea rooting the increased number of rooted microshoots of Goluboy Desert was demonstrated with the increasing of IAA concentration (from 0.5 to 3.0 mg L−1). Meanwhile, Diana and Yugana better rooted on the medium containing 1.0 mg L−1 IAA but Zolushka reached its highest rooting percentage on the medium supplemented with 0.5 mg L−1 IAA. The assessment of second-year plants undergone the whole cycle of clonal micropropagation was carried out 85% of these plants began to bear fruits.

scholarly journals Moringa oleifera L. Extracts as Bioactive Ingredients That Increase Safety of Body Wash Cosmetics

2020 ◽  
Vol 2020 ◽  
pp. 1-14
Author(s):  
Zofia Nizioł-Łukaszewska ◽  
Dominika Furman-Toczek ◽  
Tomasz Bujak ◽  
Tomasz Wasilewski ◽  
Zofia Hordyjewicz-Baran
Keyword(s):  
Oxidative Stress ◽  
Leaf Extract ◽  
Moringa Oleifera ◽  
Skin Irritation ◽  
Tree Leaves ◽  
Model Formulation ◽  
Different Types ◽  
Bioactive Ingredients ◽  
Positive Effect

The work attempts to obtain a multifunctional plant extract derived from Moringa tree leaves. Obtained results indicate a strong antioxidant potential of the tested extracts. It was shown that Moringa oleifera leaf extract is a rich source of flavonoid and phenolic compounds. Furthermore, it shows a strong antioxidant activity by scavenging free radicals. In vitro toxicity studies showed that the tested extracts in concentrations up to 5% showed a positive effect on cell proliferation and metabolism and may contribute to the reduction of oxidative stress in cells. It was noted that the tested model formulation of cosmetic (1% SCS) with the addition of different types of extracts might contribute to the reduction of skin irritation and improve the safety of the product.

scholarly journals INDUCING THE IN VITRO ROOTING OF SAGO PALM (Metroxylon sagu Rottb.) USING AUXIN

2015 ◽  
Vol 2 (2) ◽  
pp. 73
Author(s):  
Teuku Tajuddin ◽  
Karyanti . ◽  
Tati Sukarnih ◽  
Nadirman Haska
Keyword(s):  
Large Scale ◽  
Clonal Propagation ◽  
Positive Response ◽  
In Vitro Rooting ◽  
Root Induction ◽  
Sago Palm ◽  
Different Types ◽  
Superior Genotypes ◽  
Metroxylon Sagu

Tanaman sagu (Metroxylon sagu Rottb.) memiliki potensi yang besar sebagai sumber pangan, energi dan bahan baku industri. Kultur jaringan tanaman sagu telah dilakukan di Balai Pengkajian Bioteknologi BPPT dalam rangka perbanyakan genotipe atau aksesi unggul secara massal. Namun demikian, kendala utama yang dihadapi pada perbanyakan in vitro tanaman sagu adalah sulitnya pembentukan akar. Penelitian ini bertujuan untuk mendapatkan kombinasi hormon yang tepat dalam menginduksi perakaran tanaman sagu in vitro. Tunas anakan muda (15-20 cm) yang diperoleh dari daerah Rangkasbitung, Provinsi Banten digunakan sebagai eksplan. Dalam penelitian ini perakaran in vitro diinduksi dengan berbagai perlakuan jenis dan konsentrasi hormon auksin, konsentrasi medium dan jenis agar. Sebagai medium dasar digunakan medium Gamborg. Hasil penelitian menunjukkan bahwa konsentrasi IBA dan NAA yang terbaik adalah pada taraf 35 ppm. Selanjutnya Gelrite memberikan respon yang positif dengan munculnya perakaran pada pangkal eksplan.Kata Kunci: Induksi perakaran,  jenis agar, kultur in vitro, auksin, sagu ABSTRACTSago palm (Metroxylon sagu Rottb) has huge potential as food, energy and industrial bioresources. In vitro culture of sago palm was performed in Biotech Center, BPPT in order to obtain a large-scale of mass clonal propagation of superior genotypes. Nevertheless, the main obstacle for the sago palm in vitro propagation was rooting formation. The purpose of our study was to obtain the best hormones combination for root induction on sago palm shoots in vitro. The young suckers (15-20 cm) obtained from Rangkasbitung area, Banten Province, were used as explants. In our study, in vitro rooting was induced by different types and concentrations of auxin, medium strength and solidifying agents. The shoots were cultured on Gamborg media. The result showed that the best level of both hormones IBA and NAA for root induction was 35 ppm. Moreover the solidifying agent of Gelrite gave positive response by stimulating root at the basal-end.Keywords: Rooting induction, solidifying agent, in vitro cultures, auxin, sago palm

scholarly journals IN VITRO ROOTING OF PINUS STROBUS AND THUJA OCCIDENTALIS `HETZ'S WINTERGREEN' AS INFLUENCED BY ANTIGIBBERELLIN COMPOUNDS (ANCYMIDOL AND FLURPRIMIDOL).

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1137G-1138
Author(s):  
Len Burkhart ◽  
Martin Meyer ◽  
S. Dorner
Keyword(s):  
In Vitro Culture ◽  
Root Formation ◽  
Pinus Strobus ◽  
In Vitro Rooting ◽  
Pulse Treatment ◽  
White Pine ◽  
Thuja Occidentalis ◽  
Lake States

Rooting of shoots from in vitro culture of most conifers can be difficult. An antigibberellin, ancymidol, has been shown to promote rooting of in vitro proliferated shoots of asparagus clones, but it has not been tested on conifers. Ancymidol and flurprimidol was tested for rooting on established cultures of Lake States white pine (Pinus strobus). Pulse treatments containing 5 uM ancymidol and 0.5 uM NAA gave 43% rooting, while pulse treatment with 0.5 uM NAA resulted in 7% root formation. Flurprimidol also stimulated root formation on white pine shoots, but was less than ancymidol. Thuja occidentalis `Hetz's Wintergreen' formed roots on 87% of in vitro proliferated shoots when given a pulse treatment with 5 uM ancymidol and 50 uM NAA. Shoots initiated an average of 10 roots after 60 days on vermiculite containing 1/2 liquid MCM medium.

scholarly journals Clonal micropropagation of bog cranberry (Vaccinium oxycoccos L.) in the Udmurt Republic

2021 ◽  
Vol 22 (1) ◽  
pp. 57-66
Author(s):  
E. N. Cheremnykh ◽  
T. G. Lekontseva ◽  
A. V. Khudyakova ◽  
A. V. Fedorov
Keyword(s):  
In Vitro Culture ◽  
Nutrient Medium ◽  
Root Formation ◽  
Sphagnum Moss ◽  
Multiplication Factor ◽  
Clonal Micropropagation ◽  
Planting Material ◽  
Udmurt Republic

The paper presents the results of 2018-2019 research on improving the technology of growing planting material of bog cranberry (Vaccinium oxycoccos L.) of Krasa Severa, Severyanka, Virussaare varieties on the basis of in vitro. Studied was the effect of the concentrations of growth regulators in the composition of the nutrient medium according to Anderson's recipe on the reproduction and subsequent rooting of micro cuttings, as well as the duration of cultivation and adaptation of micro plants depending on partial pruning of shoots. It has been established that at the stage of introduction into in vitro culture, sterilization of explants with 33% hydrogen peroxide in an exposure of 5-8 minutes with washing in 5 portions of sterile distillate gives 60-80 % of viable shoots. The optimum phase of plant development for the successful introduction of in vitro culture is the swelling of buds. Cultivation of micro cuttings was carried out in a light room at a temperature of 25±2 °С, a photoperiod of 16 hours. The duration of each subculturing was 30-60 days. For the stage of actual micropropagation on Anderson's nutrient medium, an increase in the dose of cytokinin 6-benzylaminopurine (6-BAP) from 0.2 to 0.5 mg/l and an increase in the duration of cultivation from 30 to 60 days contributed to a significant increase in the multiplication factor on average for the tested cranberry varieties.According to the efficiency of micropropagation, the varieties Virussaare and Krasa Severa were distinguished – 9.3-12.0 pcs/stalk, respectively. At the rooting stage, the use of a root-forming reagent of indolyl-3-acetic acid (IUK) in doses of 0.2, 0.5 and 1.0 mg/l in the composition of Anderson's nutrient medium did not affect the quality of root formation and the length of shoots of Virussaare micro-plants. No significant varietal differences in the root-forming ability of microcuttings were found. The tendency of better rooting of micro cuttings was observed in the Virussaare variety (90.3 %) compared to the Severyanka (85.7 %) and Krasa Severa (79.3 %) varieties. Micro plants of the Krasa Severa cultivar were characterized by the longest shoots, the total number of roots was less, but their length was longer in comparison with other cultivars. For the adaptation stage, a substrate from a mixture of lowland peat and sphagnum moss was used (1:1). The efficiency of adaptation of micro plants of cranberry varieties when cutting the tip of the shoots was 100 %. Pruning of micro plants shoots contributed to the formation of more side shoots and better development of the aboveground part of the plants.

Different Co-Culture Systems Have a Useful Impact on Preimplantation Embryo Development

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Samy R ◽  
◽  
Ghanem N ◽  
Keyword(s):  
Stem Cells ◽  
Embryo Development ◽  
Preimplantation Development ◽  
Cell Contact ◽  
Embryonic Fibroblasts ◽  
Preimplantation Embryo Development ◽  
Different Types ◽  
Embryo Metabolism ◽  
Positive Effect

The production of in vitro produced embryos of good morphological quality and viability is a prerequisite for successful assisted reproduction biotechnologies in animal breeding and human. The co-culturing system has been applied to improve preimplantation development that could subsequently resulted in successful pregnancy. There are different types of reproductive and non-reproductive cells that have been used during preimplantation development. The most well-known reproductive cells are those recovered from ovaries (cumulus and granulosa cells), oviduct and endometrium cells. While, in last decade stem cells such as mesenchymal stem cells and murine embryonic fibroblasts that originated from different tissues have been used to support early embryonic development. The positive effect co-culturing system was suggested to be due to direct mechanical cell-to-cell contact that occurred between the dividing embryos embryo and helper cells in addition to secretions of various bioactive biological components like growth factors and scavenging the deleterious byproducts that resulted from embryo metabolism. In current review, we will highlight the effects of different couture systems on embryo development and their suggested mechanisms to exert the beneficial impacts.

scholarly journals In vitro root formation and plantlet development in dendrobium orchid

1970 ◽  
Vol 35 (2) ◽  
pp. 257-265 ◽  
Author(s):  
MM Khatun ◽  
H Khatun ◽  
D Khanam ◽  
Md Al-Amin
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Root Length ◽  
Root Formation ◽  
Combined Effect ◽  
In Vitro Rooting

The experiment was conducted to investigate the combined effect of different plant growth regulators with and without charcoal supplementation for root formation and plantlet development from protocorm like bodies (PLBS) of orchid. The combination of BAP + NAA, BAP + IAA, BAP + IBA, and IAA + IBA at different concentrations were studied. It revealed that the highest number of roots was obtained from 1.0 mg/L each of IAA + IBA combination (6.667) and the highest root length was recorded from 2.0 mg/L BAP + 1.0 mg/L IBA with charcoal supplementation. The treatment combinations, 1.0 mg/L each of BAP + NAA, BAP + IAA, BAP + IBA, and IAA + IBA were found best for producing more rooted plantlets with charcoal supplementation. It revealed that charcoal enhanced the root formation. Keywords: Orchid; Dendrobium; hybrid; In vitro rooting. DOI: 10.3329/bjar.v35i2.5888Bangladesh J. Agril. Res. 35(2) : 257-265, June 2010

scholarly journals ВМІСТ ФОТОСИНТЕТИЧНИХ ПІГМЕНТІВ У РОСЛИНАХ РОДУ CARLINA L. У ПРИРОДІ ТА КУЛЬТУРІ IN VITRO

2020 ◽  
Vol 78 (4) ◽  
pp. 16-23
Author(s):  
N. B. Kravets ◽  
L. R. Hrytsak ◽  
M. Z. Prokopyak ◽  
O. Yu. Mayorova ◽  
N. M. Drobyk
Keyword(s):  
Acid Solution ◽  
Growth Regulators ◽  
Root Formation ◽  
Cultivated Plants ◽  
Presowing Treatment ◽  
Murashige Skoog ◽  
Half Strength ◽  
Semi Solid ◽  
Positive Effect

im. The aim of the study was to choose conditions for rooting improvement of in vitro cultivated plants of some species of Carlina L. genus. Methods. For receiving and rooting of aseptic sprouts, seeds of Carlina acaulis L., Carlina cirsioides Klok and Carlina onopordifolia Besser ex Szafer, Kulcz. et Pawl were subjected to presowing treatment with gibberellic acid solution (GA3) or indolebyturic acid solution (IBA). Sterilized seeds were planted in sterile Petri dishes on semi-solid Murashige, Skoog nutrient medium with half-strength concentrations of macro- and microsalts without growth regulators. Results. It was found that with the seed soaking of C. acaulis, C. cirsioides and C. onopordifolia in GA3 solution the percentage of root formation amounted to 33.3 %, 33.3 % and 22.2 % respectively. Presowing treatment of carlina seeds in IBA solution with concentration of 1000 mg for 2–4 hours before sterilization gave a positive effect: the percentage of root formation for C. acaulis, C. cirsioides and C. onorordifolia was 2.4–4.5 times higher compared to the treatment with GA3 solution. Conclusions. To form the root system of carlina plants it is effective to soak the seeds in the solution of IBA. Thus we were able to increase the percentage of rooting of C. sirsioides and C. onorordifolia plants to 100 %, C. acaulis plants – up to 80 % and avoid sprouts’ injury and changes in the concentrations of the IBA, which may occur during sterilization at high temperatures by using non-sterile solution of growth regulators.

scholarly journals The influence of Cinnamomum zeylanicum in plant tissue culture experiments of Gardenia jasmenoides Ellis

2013 ◽  
Vol 10 (4) ◽  
pp. 1102-1107
Author(s):  
Baghdad Science Journal
Keyword(s):  
Root Formation ◽  
Culture Media ◽  
Shoot Proliferation ◽  
Naphthalene Acetic Acid ◽  
Alcoholic Extract ◽  
Fresh Weight ◽  
Benzyl Adenine ◽  
Cinnamomum Zeylanicum ◽  
Positive Effect

Cinnamon plant is considered one of important medicinal plants because it is rich with many active compounds. This research is aimed to study possible effects of extract in culture media of Gardenia jasmenoides. Alcoholic extract was prepared from the bark of cinnamon at different concentrations (0.0, 1.0, 2.0) mg/L, then added to culture media to notice the effect of these concentrations on the growth and development of tissues and organs of Gardenia jasmenoides Ellis in vitro. Results showed the positive effect of increasing callus fresh weight and shoot proliferation from single nodes with presence of plant regulators, 5.0 mg/L Naphthalene acetic acid (NAA) and 3.0 mg/L Benzyl adenine (BA). Results showed that extract has a slight effect on root formation with the presence of plant regulators or when it is alone.

scholarly journals In vitro Propagation of Globba schomburgkii Hook. f. via Bulbil Explants

2017 ◽  
Vol 15 (10) ◽  
pp. 701-710
Author(s):  
Piyaporn SAENSOUK ◽  
Surapon SAENSOUK ◽  
Phattaraporn PIMMUEN
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
In Vitro Propagation ◽  
Root Formation ◽  
Survival Rates ◽  
Shoot Formation ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Different Types

An efficient and rapid protocol for the micropropagation of Globba schomburgkii Hook. f. via bulbil explants was investigated. The long divided and undivided bubils of G. schomburgkii Hook. f. were cultured on MS medium (Murashige and Skoog) that had either 3 mg/l benzyladenine (BA) or 0.5 mg/l naphthaleneacetic acid (NAA) added for 8 weeks. The results indicated that the long divided bulbils of G. schomburgkii Hook. f. showed a greater amount of plant regeneration than the undivided bulbils. Callus induction, as well as shoot and root formation, were observed when culturing microshoots of 1 cm in length on media (MS) that had Thidiazuron (TDZ) or NAA plus BA added at a range of concentrations for 8 weeks. The highest percentage of callus induction was 40 % when culturing the microshoots on MS medium supplemented with NAA and BA. The best result for shoot formation was achieved when culturing the microshoots on MS medium with TDZ added. The highest number of roots was obtained when culturing the microshoots on MS medium with NAA and BA added. The in vitro-derived plantlets of G. schomburgkii Hook. f. were transplanted to pots containing different types of potting mixture in a greenhouse. The survival rates were 80 % when G. schomburgkii Hook. f. was transplanted to sand.

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