scholarly journals Variation of the Nuclear DNA Content of Species of Subtribe Citrinae (Rutaceae)

HortScience ◽  
1998 ◽  
Vol 33 (7) ◽  
pp. 1247-1250 ◽  
Author(s):  
Mukadder Kayum ◽  
N. Kemal Koç ◽  
Veli-Matti Rokka
Keyword(s):  
Tree Species ◽  
Nuclear Dna ◽  
Diploid Species ◽  
Citrus Fruit ◽  
Nuclear Dna Content ◽  
Fruit Trees ◽  
Fruit Tree ◽  
Poncirus Trifoliata ◽  
Significant Difference ◽  
Severinia Buxifolia

Laser flow cytometry was used to analyze nuclear DNA contents (2C values) of five genera (Severinia Ten., Atalantia Corrèa, Fortunella Swing., Poncirus Raf., and Citrus L.) taxonomically grouped in subtribe Citrinae (citrus fruit trees) of the Rutaceae. The genotypes analyzed had 2C values ranging from 0.67 pg for diploid Severinia buxifolia (Poir.) Ten. to 1.27 pg for tetraploid Hongkong Fortunella hindsii Swing. There was no significant difference in the 2C values within the sexually compatible diploid species of 11 “true citrus fruit trees” [Citrus aurantium L., C. grandis (L.) Osbeck, C. limon (L.) Burm. f., C. limonia Osbeck, C. paradisi Macf., C. reshni Hort. ex Tanaka, C. sinensis (L.) Osbeck, C. volkameriana Ten. & Pasq., Poncirus trifoliata (L.) Raf., and the intergeneric hybrid C. sinensis × P. trifoliata]. The species Atalantia ceylanica (Arn.) Oliv. (a “near-citrus fruit tree”), sexually incompatible with Citrus spp., had a 2C value significantly different from those of the true citrus fruit tree species. The 2C value of Severinia buxifolia (a “primitive citrus fruit tree”), another species sexually incompatible with the Citrus spp., also differed from those of some of the true citrus fruit tree species. The data largely corresponds with taxonomical differences between a) the genera Citrus and Poncirus and b) the genera Severinia and Atalantia, all assigned to subtribe Citrinae.

scholarly journals GROWTH OF DIFFERENT FRUIT TREE SPECIES IN SILVOPASTORAL SYSTEMS DURING THE ESTABLISHMENT PHASE

2017 ◽  
Vol 30 (4) ◽  
pp. 1040-1049 ◽  
Author(s):  
CAROLINA DELLA GIUSTINA ◽  
ROBERTA APARECIDA CARNEVALLI ◽  
MARCELO RIBEIRO ROMANO ◽  
DIEGO BARBOSA ALVES ANTONIO ◽  
CAMILA ECKSTEIN
Keyword(s):  
Tree Species ◽  
Block Design ◽  
Control Plant ◽  
Fruit Trees ◽  
Fruit Tree ◽  
Silvopastoral Systems ◽  
Area Index ◽  
Mato Grosso ◽  
Plant Accumulation ◽  
Establishment Phase

ABSTRACT The benefits of integrating agricultural components into silvopastoral systems are widely known, but the limited knowledge about ecological processes in the establishment phase impedes the use of this technology. The objective of this study was to evaluate interactions between fruit tree species and the sward layer under canopies of trees in the establishment phase of silvopastoral systems in Mato Grosso, Brazil. The experiment was implemented in October 2013, with an evaluation period from January to July 2015. The systems were composed of eight fruit trees intercropped with Tifton 85 grass. A completely randomized block design was adopted, with two replications per area per treatment. We evaluated the agronomic performance of the fruit trees, the categories of the light environment, and the plant accumulation under the canopies. The acerola fruit trees of the variety Roxinha had higher Leaf area index (LAI) and Light interception (LI) values, showing a denser canopy with small porosity and the lowest light quality available to the plants beneath the canopy (lower red/far-red ratio), thereby decreasing plant accumulation under trees. The guava fruit trees showed higher growth rates than the other fruit trees, but lower LAI and LI values and a higher red/far-red ratio, allowing higher plant growth under the canopy. Cajá trees showed a similar behavior; however, this species is deciduous, which limits its potential use in integrated systems. Banana and coconut trees were highly dependent on irrigation during the dry season. The remaining species showed an adequate growth and potential to control plant species growth under their canopies.

scholarly journals The effect of spring frosts on the nectar production and the bee visitation of fruit trees

2000 ◽  
Vol 6 (1) ◽  
Author(s):  
P. Benedek ◽  
J. Nyéki ◽  
I. Amtmann ◽  
F. Bakcsa ◽  
J. Iváncsics ◽  
...  
Keyword(s):  
Tree Species ◽  
Fruit Trees ◽  
Frost Damage ◽  
Fruit Tree ◽  
Wild Plants ◽  
Cold Weather ◽  
Nectar Production ◽  
Spring Frost ◽  
Fruit Orchards ◽  
Spring Frosts

Fruit tree species suffered very strong spring frosts in 1997 in Hungary. This caused partial or total damages at buds and flowers depending on site and time of blooming. It was demonstrated at a number of experiments that frost and cold weather also strongly affected the nectar production of surviving flowers. No or very little amount of nectar was measured in flowers first of all of early blooming fruit tree species (apricot) but also of pear and apple in some places. In spite of this fact intensive honeybee visitation was detected in the flowers of fruit trees that suffered partial frost damage only at those sites where honeybee colonies were placed in or at the experimental plantations and the lack of sufficient amount of nectar did not affected bee behaviour seriously on fruit flowers. This means that bad nectar production failed to affect bee visitation of fruit trees definitely. The reason for this was the fact that not only fruit trees but another early bee plants (wild plants, too) suffered frost damage. Accordingly, in lack of forage bees intensively searched for food at blooming fruit trees with some living flowers. Consequently, there was an acceptable yield at those plantations where bud and flower damage was not complete. Accordingly, intensive bee visitation (that is moving additional bee colonies to overpopulate fruit orchards with honeybees) can be an effective tool to decrease or eliminate the detrimental effect of spring frost on the yield of fruit trees where bud or fruit damage is not too high.  

scholarly journals Diversity Of Fruit And Timber Tree Species In The Coastal Homesteads Of Southern Bangladesh

2013 ◽  
Vol 39 (1) ◽  
pp. 83-94
Author(s):  
SK. Ahiul Islam ◽  
Md. Abdul Quddus Miah ◽  
Md. Ahsan Habib
Keyword(s):  
Tree Species ◽  
Fruit Trees ◽  
Fruit Tree ◽  
Betel Nut ◽  
Timber Tree

In a study conducted in three southern districts (Bhola, Borguna and Patuakhali) of Bangladesh, a total of 69 tree species was recorded from the homegardens, of which 32 were fruit tree and 37 were timber tree species. Among the fruit tree species, coconut, betel nut, mango, jackfruit, guava, velvety apple were found in more than 80% households. The stocking of fruit trees per homestead was found highest for betel nut (265) followed by velvety apple (212), mango (38) coconut (25), jackfruit (20) and guava (9). Among the timber tree species, rain tree, mehogoni, raj koroi were most prevalent and found in more than 65% homesteads. The stocking of timber trees/ homestead was found highest for mehogoni (79) and then for rain tree (57), raj koroi (29) and katbadam (6). J. Asiat. Soc. Bangladesh, Sci. 39(1): 83-94, June 2013 DOI: http://dx.doi.org/10.3329/jasbs.v39i1.16037

Factors influencing gene introgression into the allotetraploid Coffea arabica L. from its diploid relatives

Genome ◽  
2004 ◽  
Vol 47 (6) ◽  
pp. 1053-1060 ◽  
Author(s):  
Juan C Herrera ◽  
Marie C Combes ◽  
Hernando Cortina ◽  
Philippe Lashermes
Keyword(s):  
Coffea Arabica ◽  
Nuclear Dna ◽  
Flow Cytometric Analysis ◽  
Diploid Species ◽  
Nuclear Dna Content ◽  
Female Parent ◽  
Coffea Canephora ◽  
Triploid Hybrid ◽  
Gene Introgression ◽  
Coffea Arabica L

Factors controlling gene introgression into cultivated arabica coffee (Coffea arabica L.) were investigated. Interspecific triploid hybrid plants between the tetraploid species C. arabica (2n = 44) and a diploid species (2n = 22), either Coffea canephora or Coffea eugenioides, were backcrossed to C. arabica (male parent). Flow cytometric analysis of the nuclear DNA content revealed that most of the BC1 individuals derived from triploid hybrids involving C. eugenioides were tetraploid or nearly tetraploid. Among the gametes produced by the interspecific triploid hybrids, those possessing approximately 22 chromosomes appeared strongly favored. The amount of introgression in BC1 individuals (21 and 43 for the BC1 progenies involving C. canephora and C. eugenioides, respectively) was estimated using species-specific microsatellite markers. A large number of introgressed markers was observed in all BC1 individuals. Nevertheless, while the frequency of introgressed markers seemed as expected, assuming random chromosome segregation and diploid gamete formation, in the BC1 derived from triploid hybrids involving C. canephora, this frequency appeared significantly lower in the BC1 derived from triploid hybrids involving C. eugenioides. Furthermore, the comparison of reciprocal progenies between C. arabica and triploid interspecific hybrids (C. arabica × C. canephora) used as male or female parent revealed a very strong effect of the backcross direction.Key words: irregular meiosis, coffee, reciprocal crosses, molecular marker, triploid hybrids.

scholarly journals Use of Flying Dragon Trifoliate Orange As Dwarfing Rootstock for Citrus Under Tropical Climatic Conditions

HortScience ◽  
2012 ◽  
Vol 47 (1) ◽  
pp. 11-17 ◽  
Author(s):  
François Mademba-Sy ◽  
Zacharie Lemerre-Desprez ◽  
Stéphane Lebegin
Keyword(s):  
New Caledonia ◽  
Citrus Fruit ◽  
Tree Height ◽  
Fruit Trees ◽  
Fruit Production ◽  
Climatic Conditions ◽  
Poncirus Trifoliata ◽  
Trifoliate Orange ◽  
Gross Margin ◽  
Trunk Diameter

Citrus fruit trees grown under tropical climatic conditions have a high level of vigor and, consequently, late fruit-bearing and low productivity. The use of Flying Dragon trifoliate orange [Poncirus trifoliata var. monstrosa (T.Itô) Swing.] (FD) rootstock could overcome these negative effects by inducing small trees with early production. Trials including eight commercial cultivars began in Dec. 1992 on an irrigated plot on the main island of New Caledonia (South Pacific). Growth of the trees was observed over a 13-year period through twice-yearly measurements of tree height, canopy between and in the rows, and trunk diameter. Fruit production was recorded beginning 2 years after planting. According to the climatic and pedological conditions of the experimental site, trees grafted on FD could, depending on cultivar, be planted in densities from 519 to 1111 trees/ha. Over the 13 years, yields were 0.5 to 2.8 times greater than those of the same cultivar on the standard rootstock. ‘Tahiti’ lime cumulative planting and maintenance costs were only 1.5 times higher for a density five times as great, and the level of productivity per hectare (gross margin/ha) was 3.3 times that of traditional orchards. Grafting citrus cultivars on FD, which is seldom used in the Mediterranean zone as a result of its excessive dwarfing effect in relation with the climatic conditions, could prove, on the other hand, promising in tropical areas.

Computer extracted nuclear features from tumor and benign regions of Feulgen and H&E images to help predict recurrence in prostate cancer patients following radical prostatectomy.

2017 ◽  
Vol 35 (15_suppl) ◽  
pp. e16556-e16556
Author(s):  
Anna Gawlik ◽  
George Lee ◽  
Michael D Feldman ◽  
Guangjing Zhu ◽  
Robert W. Veltri ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Radical Prostatectomy ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Nuclear Shape ◽  
Rank Test ◽  
Surgical Patient ◽  
Nuclear Morphology ◽  
Texture Intensity ◽  
Significant Difference

e16556 Background: Following radical prostatectomy, around 30% of prostate cancer (PCa) patients experience biochemical recurrence (BCR). H&E highlights nuclear morphology and Feulgen reflects nuclear DNA content, a feature linked to PCa presence and aggressiveness. In this work we sought to explore whether computer extracted measurements of tumor morphology and tumor adjacent benign regions on H&E and Feulgen tissue images could predict BCR. Methods: We used 108 patients (59 BCR and 49 non-recurrence (NR)) and each patient had 242 QH features calculated from both the tumor and benign region of stained TMA core images. Feature selection was performed on a training set (30 BCR, 24 NR) to select the 10 most discriminating tumor and tumor adjacent benign features of each stain. A random forest classifier was trained with features so identified and validated on a test set (29 BCR, 25 NR) to predict BCR. Predictions were displayed using Kaplan-Meier analysis and area under the ROC curve (AUC). Results: The most discriminating feature from the tumor regions of the H&E stain was Fourier descriptors of nuclear shape and from the Feulgen stain was texture intensity while from the benign regions it was invariant moments of nuclear shape and texture contrast energy. Combining the significant features from tumor and tumor adjacent benign regions from H&E and Feulgen resulted in the highest accuracy and a statistically significant difference (p < 0.05) via a log-rank test (Table 1). Gleason score did not show statistically significant differences and had the lowest AUC. Conclusions: Combining nuclear morphology and DNA related features of the tumor and tumor adjacent benign regions enabled accurate prediction of BCR. With additional multi-site validation, the combined H&E + Feulgen classifier could allow better risk stratification and post-surgical patient management. [Table: see text]

Genome size and genome evolution in diploid Triticeae species

Genome ◽  
2007 ◽  
Vol 50 (11) ◽  
pp. 1029-1037 ◽  
Author(s):  
T. Eilam ◽  
Y. Anikster ◽  
E. Millet ◽  
J. Manisterski ◽  
O. Sagi-Assif ◽  
...  
Keyword(s):  
Genome Size ◽  
Intraspecific Variation ◽  
Dna Content ◽  
Nuclear Dna ◽  
Diploid Species ◽  
Nuclear Dna Content ◽  
Dna Amount ◽  
Nuclear Dna Amount ◽  
Speciation Event ◽  
Close Temporal Proximity

One of the intriguing issues concerning the dynamics of plant genomes is the occurrence of intraspecific variation in nuclear DNA amount. The aim of this work was to assess the ranges of intraspecific, interspecific, and intergeneric variation in nuclear DNA content of diploid species of the tribe Triticeae (Poaceae) and to examine the relation between life form or habitat and genome size. Altogether, 438 plants representing 272 lines that belong to 22 species were analyzed. Nuclear DNA content was estimated by flow cytometry. Very small intraspecific variation in DNA amount was found between lines of Triticeae diploid species collected from different habitats or between different morphs. In contrast to the constancy in nuclear DNA amount at the intraspecific level, there are significant differences in genome size between the various diploid species. Within the genus Aegilops , the 1C DNA amount ranged from 4.84 pg in A. caudata to 7.52 pg in A. sharonensis; among genera, the 1C DNA amount ranged from 4.18 pg in Heteranthelium piliferum to 9.45 pg in Secale montanum . No evidence was found for a smaller genome size in annual, self-pollinating species relative to perennial, cross-pollinating ones. Diploids that grow in the southern part of the group’s distribution have larger genomes than those growing in other parts of the distribution. The contrast between the low variation at the intraspecific level and the high variation at the interspecific one suggests that changes in genome size originated in close temporal proximity to the speciation event, i.e., before, during, or immediately after it. The possible effects of sudden changes in genome size on speciation processes are discussed.

Apple SSRs present in coding and noncoding regions of expressed sequence tags show differences in transferability to other fruit species in Rosaceae

2013 ◽  
Vol 93 (2) ◽  
pp. 183-190 ◽  
Author(s):  
Ying Zhou ◽  
Jing Li ◽  
Schuyler S. Korban ◽  
Yuepeng Han
Keyword(s):  
Expressed Sequence Tags ◽  
Tree Species ◽  
Fruit Trees ◽  
Fruit Tree ◽  
Noncoding Regions ◽  
Fruit Species ◽  
Pcr Products ◽  
Rosaceae Family ◽  
Expressed Sequence ◽  
Simple Sequence

Zhou, Y., Li, J., Korban, S. S. and Han, Y. 2013. Apple SSRs present in coding and noncoding regions of expressed sequence tags show differences in transferability to other fruit species in Rosaceae. Can. J. Plant Sci. 93: 183–190. Simple sequence repeat markers derived from expressed sequence tags (ESTs) are referred to as eSSRs. To develop molecular markers for non-model plants in Rosaceae, we investigated the transferability of apple eSSRs across seven fruit trees, belonging to four genera and 11 species of the Rosaceae family, including peach, quince, pear, loquat, apricot, cherry, and plum. Of the 98 apple eSSRs tested, 86 successfully amplified PCR products in at least one of the fruit tree species. Five apple eSSRs produced amplicons in more than five fruit tree species, and were deemed as a widely transferable Rosaceae marker set. Frequency of transferability of apple eSSRs across all seven fruit trees of Rosaceae varied widely among genera and species, with an average transferability of 29.0%. Overall, apple eSSRs transferred more easily to peach and pear than to plum and loquat. Interestingly, apple eSSRs present in coding sequences (CDS) showed higher levels of transferability to other fruit trees than those present in noncoding or untranslated regions (UTRs). Interestingly, apple eSSRs present in 5'UTRs showed lower frequencies of transfer than those present in 3'UTRs. The latter finding suggested that 5'UTRs might have diverged more rapidly than 3'UTRs in Rosaceae.

scholarly journals Determining Ploidy Level and Nuclear DNA Content in Rubus by Flow Cytometry

2002 ◽  
Vol 127 (5) ◽  
pp. 767-775 ◽  
Author(s):  
Rengong Meng ◽  
Chad Finn
Keyword(s):  
Flow Cytometry ◽  
Pacific Northwest ◽  
Ploidy Level ◽  
Dna Content ◽  
Chromosome Numbers ◽  
Nuclear Dna ◽  
Diploid Species ◽  
Nuclear Dna Content ◽  
Dna Flow Cytometry ◽  
Ploidy Levels

Nuclear DNA flow cytometry was used to differentiate ploidy level and determine nuclear DNA content in Rubus. Nuclei suspensions were prepared from leaf discs of young leaves following published protocols with modifications. DNA was stained with propidium iodide. Measurement of fluorescence of 40 genotypes, whose published ploidy ranged from diploid to dodecaploid, indicated that fluorescence increased with an increase in chromosome number. Ploidy level accounted for 99% of the variation in fluorescence intensity (r2 = 0.99) and variation among ploidy levels was much higher than within ploidy levels. This protocol was used successfully for genotypes representing eight different Rubus subgenera. Rubus ursinus Cham. and Schldl., a native blackberry species in the Pacific Northwest, which has been reported to have 6x, 8x, 9x, 10x, 11x, and 12x forms, was extensively tested. Genotypes of R. ursinus were predominantly 12x, but 6x, 7x, 8x, 9x, 11x, and 13x forms were found as well. Attempts to confirm the 13x estimates with manual counts were unsuccessful. Ploidy level of 103 genotypes in the USDA-ARS breeding program was determined by flow cytometry. Flow cytometry confirmed that genotypes from crosses among 7x and 4x parents had chromosome numbers that must be the result of nonreduced gametes. This technique was effective in differentiating chromosome numbers differing by 1x, but was not able to differentiate aneuploids. Nuclear DNA contents of 21 diploid Rubus species from five subgenera were determined by flow cytometry. Idaeobatus, Chamaebatus, and Anaplobatus were significantly lower in DNA content than those of Rubus and Cylactis. In the Rubus subgenus, R. hispidus and R. canadensis had the lowest DNA content and R. sanctus had the highest DNA content, 0.59 and 0.75 pg, respectively. Idaeobatus had greater variation in DNA content among diploid species than the Rubus subgenus, with the highest being from R. ellipticus (0.69 pg) and lowest from R. illecebrosus (0.47 pg).

scholarly journals The effect of the limitation of insect pollination period on the fruit set and yield of temperate-zone fruit tree species

2000 ◽  
Vol 6 (1) ◽  
Author(s):  
P. Benedek ◽  
J. Nyéki ◽  
M. Soltész ◽  
Z. Erdős ◽  
I. Skola ◽  
...  
Keyword(s):  
Tree Species ◽  
Fruit Set ◽  
Fruit Trees ◽  
Fruit Tree ◽  
Temperate Zone ◽  
Bee Pollination ◽  
Fruit Species ◽  
The Individual ◽  
Compatible Pollen ◽  
Bee Colonies

The duration of effective bee pollination period was limited by caging flowering branches for shorter or longer time in blooming fruit trees in a number of experiments during the past decades. In the case of self-sterile fruit species and cultivars (apples, pears, quinces, some plums, some sour cherries) even partial limitation of the effective duration of bee pollination period significantly reduced the fruit set and the yield. In the case of self-fertile apricots the effect of the total and also the influence of partial limitation of bee pollination period was the same as in the case of the mentioned self-sterile fruits. On the other hand, in the case of another self-fertile fruits (some plums, some sour cherries), the effect of partial limitation of bee pollination period was usually small, but complete (or incomplete but strong) limitation of be pollination usually resulted in a strong reduction of yield. This means that not only self-sterile but also self-fertile fruits clearly depend on insect (bee) pollination. This is because pollen dehiscence of anthers and the receptive period of stigmas do not overlap in time within the individual flowers. Stigmas in self-fertile trees, therefore, need pollen carried by bees from another flowers of the same tree (or compatible pollen from another trees). Accordingly, additional bee pollination (moving bee colonies to the orchards in flower) is needed to all kinds of temperate-zone fruit tree species when bee visitation of plantations is not abundant enough for some reasons.

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