scholarly journals 044 Selection for Floret Glucoraphanin Concentration Among Inbred Broccoli

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 448E-448
Author(s):  
Mark W. Farnham ◽  
Jed W. Fahey ◽  
Katherine K. Stephenson

Broccoli (Brassica oleracea L. Italica Group) is a rich source of the aliphatic glucosinolate glucoraphanin. The glucoraphanin breakdown product, sulforaphane, has been shown to induce Phase II detoxication enzymes (e.g., Quinone Reductase) and has attracted attention as a potential chemoprotector against cancer. The objectives of this research were to evaluate the concentration of glucoraphanin in an array of diverse broccoli inbreds (doubled-haploids) largely derived from commercial germplasm and to determine if expression of glucoraphanin level in this initial evaluation is correlated with expression in a subsequent environment. In 1996, individual florets from single broccoli heads were sampled from 75 inbred lines grown in the field at Charleston, S.C., and glucoraphanin concentration was assayed. In this test, concentrations ranged from 0.04 to 2.94 μmol glucoraphanin per g fresh weight of florets and the mean concentration was 0.86. In 1997, a subset of 22 inbreds analyzed the first year were grown again in a replicated field trial. This inbred subset was made up of lines with diverse pedigrees and with high, low, or intermediate glucoraphanin concentrations. In this second year, glucoraphanin concentration had a range from 0.24 to 2.99 μmol per g fresh weight of florets and a mean of 1.37. Correlation of entry mean glucoraphanin concentration in 1997 with that in 1996 was positive (r = 0.79) and highly significant (P < 0.001) indicating that floret glucoraphanin concentration was relatively consistent between years. These observations provide evidence that floret glucoraphanin concentration has a significant genetic component.

2019 ◽  
Vol 103 (11) ◽  
pp. 1624-1632
Author(s):  
Cemal Özsaygili ◽  
Sengul Ozdek ◽  
Mehmet Cuneyt Ozmen ◽  
Hatice Tuba Atalay ◽  
Duygu Yalinbas Yeter

PurposeTo describe the long-term anatomical and functional results of surgery for retinal detachment (RD) associated with stage 4 retinopathy of prematurity (ROP) and patient and surgery-related factors affecting postoperative success.DesignRetrospective case series at a single tertiary referral paediatric vitreoretinal practice.MethodsOne hundred and twenty-one eyes of 82 infants (40 female/42 male) who underwent lens-sparing vitrectomy (LSV) or lensectomy with vitrectomy surgery for stage 4A and 4B ROP at Gazi University Department of Ophthalmology between 2011 and 2016 were enrolled in this study. Patient characteristics including gestational age, birth weight, gender, stage of ROP at presentation, preoperative treatment (laser, anti-vascular endothelial growth factor (VEGF) or combined), anatomical and functional outcome and complications were recorded. The effect of birth weight, gestational age, presence of plus disease, preoperative treatment status, surgically induced posterior hyaloid detachment, postoperative vitreous haemorrhage and iatrogenic retinal tear formation on anatomical and functional results was evaluated.Results61.2% of the eyes were stage 4A and 38.8% were stage 4B ROP. The mean follow-up was 24.5 months. 18.2% of the eyes had no preoperative treatment. Anatomical success was 86.5% for stage 4A and 68.1% for stage 4B at the first year, 91.7% for stage 4A and 69.4% for stage 4B at the second year, and 95.8% for stage 4A and 57.9% for stage 4B at the third year. Functional success was 85.1% for stage 4A and 65.9% for stage 4B at the first year, 89.6% for stage 4A and 61.1% for stage 4B at the second year, and 87.5% for stage 4A and 57.8% for stage 4B at the third year. The mean visual acuity was 1.12±0.34 logarithm of the minimum angle of resolution (logMAR) for stage 4A and 1.34±0.32 logMAR at the 3-year follow-up duration (p>0.05). There was preoperative plus disease in 59.5% of the eyes. Subsequent retinal surgeries were required in 17.4% of the eyes. Presence of plus disease and absence of preoperative treatment, iatrogenic retinal tear formation and postoperative vitreous haemorrhage were found to have significant negative effects, while surgical induction of posterior hyaloid detachment and sparing the lens intraoperatively affected the anatomical and functional results positively.ConclusionsSurgery for stage 4 ROP-associated RD resulted in encouraging anatomical and functional outcomes and the results are even better in eyes with preoperative (laser/anti-VEGF) treatment, LSV and surgically induced posterior hyaloid detachment.


1967 ◽  
Vol 69 (2) ◽  
pp. 225-230 ◽  
Author(s):  
D. A. Lambert

1. Plants of S.48 timothy were established on a 1 ft square pattern for production of seed. Two series of plots, one irrigated and one not irrigated, were treated with three levels of applied nitrogen: 0 (NO), 87 (Nl) and 261 (N2) lb N per acre (0, 97·5 and 292·5 kg N/ha) per annum, for 3 years. In this, the first of two papers, only vegetative aspects of growth are considered. Since the irrigation treatment started only 2 weeks before most of the data were recorded, effects of irrigation on vegetative growth were generally small.2. The number of tillers per plant was increased by application of nitrogen; the lower increment was more effective than the additional nitrogen in the higher dose.3. The weight of tillers per plant was increased by nitrogen at both levels of application. The mean weight of each tiller was increased by nitrogen after the first year, predominantly in the N 2 treatment. In the second year, irrigation also increased the weight per tiller.4. The weight of roots per plant was significantly affected by application of nitrogen. In the first year, the weight was increased by nitrogen in irrigated plots; in the second year the roots were significantly heavier in the Nl treatment than in N0 and N2 treatments, where weights were similar; the same situation existed in irrigated plots in the third year, but in non-irrigated plots the weights of roots were similar in N1 and N2 treatments and were heavier than in the N0 treatment. Irrigation had positive effects on the weight of roots in the first and second years.


1992 ◽  
Vol 70 (6) ◽  
pp. 1114-1119 ◽  
Author(s):  
Christopher R. Matlack ◽  
Alison J. Evans

A study was conducted from 1980 to 1982 on the diet and condition of bobcats, Lynx rufus, in the province of Nova Scotia. Skinned carcasses were provided by trappers, hunters, fur buyers, and taxidermists. The autumn–winter diet was determined from analysis of 1099 stomachs. Canine teeth were sectioned and aged to group the bobcats into three age-classes: juvenile, yearling, and adult. Snowshoe hare, Lepus americanus, occurred most frequently as a prey item, followed by white-tailed deer, Odocoileus virgìnianus, and small mammals. The mean weights of hare and deer consumed per feeding were similar. Male bobcats consumed deer more frequently and in larger quantities than did females. Adults consumed deer more frequently and in larger quantities than did juveniles. Bobcats harvested in the first year of the study (1980–1981) consumed deer more frequently than did those harvested in the second year (1981–1982), while hare population levels were found to be lower in the first year than in the second. A condition index was developed by dividing the sum of the weights of mesenteric, kidney, and sternum fat by the weight of the skinned carcass. The groups (males, adults, and specimens harvested in 1980–1981) that more frequently consumed deer were found to be in the best condition.


2020 ◽  
Author(s):  
George (Jeb) S Brown ◽  
Christophe Cazauvieilh

Abstract: Aim, Methods, Results, DiscussionAim: This paper presents analyses of outcome data for 317 therapists treating 14,161 patients over a three-year period to determine if therapists’ effect sizes increased over time. Each therapist treated at least 5 patients in each of their first two years of using outcome measures. Multiple outcome questionnaires were employed. All measures also included a brief alliance scale administered concurrently. Method: A severity adjusted effect size was calculated for each patient using intake scores and diagnostic group as predictors. The mean severity adjusted effect size for each therapist was calculated for their first and second years of using the outcome tools. This was done using a hierarchical linear model to control for sample size in each year, with a minimum sample of 5 cases in each year. Therapist engagement in receiving feedback was measured by counting the number of times the therapist logged into the online platform to view their results in each of the two years.Results: Therapists who logged in the view their data at least 24 times in the second year (n=123; 37%) averaged .92 effect size compared to .82 effect size for those seen by therapists who reviewed their results less frequently (n=214; 63%). Login frequency during the first year was not predictive of effect size during the second year. Discussion: The data provides evidence that effect sizes can trend upwards with measurement and feedback. Therapists’ engagement in receiving feedback appears to increase the likelihood of effect size gain.


BMJ Open ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. e035690
Author(s):  
Xiong Ye ◽  
Haiying Guo ◽  
Zhou Xu ◽  
Hui Xiao

ObjectivesEmpathy education is very important for medical students. There is little research on the influence of early clinical practice on the development of empathy and other aspects of professionalism in medical students. The aim of this study was to compare the self-reported empathy levels of first-year and second-year undergraduate medical students before and after their early clinical contact curriculum.SettingThe study was conducted at the Shanghai University of Medicine & Health Sciences, Shanghai, China.ParticipantsA total of 257 undergraduate medical students participated in the study. The 154 first-year students were studying in 10 community-based teaching hospitals, and the 103 second-year students were studying in 3 university-affiliated hospitals.Primary and secondary outcome measuresPrimary measures: the Jefferson Scale of Empathy—Student version (JSE-S) was compared between students of different sexes and in different academic years before their early clinical contact course. Secondary measures: comparisons were made after they finished the curriculum 3 weeks later.ResultsA total of 219 of 257 students responded (85.21% response rate), and 214 answers could be analysed (135 first-year and 79 second-year students; 120 female and 94 male individuals). No significant differences in the empathy scores before early clinical contact were observed between students of different sexes and in different academic years. After early clinical contact, the mean JSE-S score of the participants was significantly higher than the mean score at the beginning of the curriculum.ConclusionsEmpathy-focused training during early clinical contact can improve the empathetic capacity of undergraduate medical students. Fostering empathetic attitudes among undergraduate medical students is necessary for the early stage of their medical education. Further research is needed on the long-term effects of empathy-focused education in entry-level medical students.


PEDIATRICS ◽  
1976 ◽  
Vol 58 (4) ◽  
pp. 607-613
Author(s):  
Anna-Beth Doyle

The frequency of reported illness in children enrolled in a day-care center was compared to frequency in home-reared children and in children in family day-care homes. Children ranged from 6 to 42 months of age (mean, 21 months). Data on seven categories of illness were collected by bimonthly telephone interviews with parents during two consecutive winters. In the first year of the study gastrointestinal and total illnesses were reported significantly more frequently in children enrolled in the day-care center, though major illnesses were not. In the second year of the study illnesses were significantly more frequently reported in center children though mainly at the younger ages, that is, prior to 2 years of age. The mean numbers of illnesses and symptoms reported per call over the 2 years of the study were 2.56, 1.36, and 1.35 for center, home-reared and family day-care children respectively. Though it has been shown elsewhere that psychological health may be unaffected by early group care, the present findings imply that physical health may be somewhat reduced. It is unknown whether these children will be more resistant to infection at a later age.


2006 ◽  
Vol 54 (5) ◽  
pp. 369 ◽  
Author(s):  
Natalia Atkins ◽  
Roy Swain ◽  
Susan M. Jones

Niveoscincus microlepidotus is a viviparous lizard that exhibits biennial reproduction: births in spring optimise offspring survival in this species’ alpine habitat. Over three annual activity seasons, we investigated the hypothesis that date of birth influences offspring characteristics. In the first year of our study, there was no effect of date of birth on any offspring characteristic measured. In the second year, later-born offspring grew significantly faster. In the third year, later-born offspring were significantly longer and heavier at birth. These varying results may be attributable to differences in basking opportunities for the gestating females due to annual variations in spring weather patterns. To test the hypothesis that both lecithotrophic (yolk) and placentotrophic nutrients support embryos during their extended gestation, we determined the degree of placentotrophy for this species. The mean dry neonate : dry egg ratio of 0.755 indicates that this species is predominantly lecithotrophic. Only in the first year of the study did the data suggest a potential for facultative placentotrophy in this species. We therefore suggest that in N. microlepidotus lecithotrophic nutrition is converted into embryonic fat bodies that support the embryo in utero over winter until spring parturition.


2014 ◽  
Vol 38 (1) ◽  
pp. 42-45 ◽  
Author(s):  
Sushma Pande ◽  
Santosh Pande ◽  
Vrushali Parate ◽  
Sanket Pande ◽  
Neelam Sukhsohale

Poor awareness among medical graduates about basic life support (BLS) is a matter of great concern. The presence of a trained rescuer is the key determinant of ultimate survival from life-threatening emergencies. To achieve this goal, early exposure to such life-saving skills is the right decision to foster these skills for medical students, which can be reenforced in succeeding years. Forty-two first-year medical students participated in this study. The entire procedure consisted of faculty training, assessment of knowledge of students by a pretest questionnaire, a lecture, a demonstration, and hands-on training using a mannequin (with special emphasis on the site, depth, rate, and sustainment of uninterrupted chest compressions). Posttest 1 was conducted to assess the knowledge gained. The retention of knowledge and skills in the second year was evaluated by posttest 2 and directly observed procedural skills, respectively. Student feedback was collected on five-point Likert scale. Analysis using a Freidman test indicated the mean rank for posttest 1 (2.81) to be significantly higher than the pretest (1.26), indicating a gain in knowledge. The mean rank for posttest 2 (1.93) was lower than for posttest 1 (2.81) but was significantly higher compared with the pretest (1.26), indicating a significant retention of knowledge during the second year. Directly observed procedural skill evaluation showed that 7% students could perform all the seven steps correctly and that 74% students could perform three or more steps correctly, signifying a good retention of skill. Two students taught BLS skills to their family members as well. The results of this study suggest that the program provides students with sound basic knowledge and adequate practical skills in BLS.


2021 ◽  
Vol 2 ◽  
pp. 246
Author(s):  
Hardianti Hardianti ◽  
Jamaluddin Ahmad ◽  
Syam Hermansyah

This research aimed to investigate the constructivist approach to enhance students reading comprehension at UMS Rappang. This research employed the pre-experimental method with one group pre-test and post-test design. The population was the second-year students of UMS Rappang in 2018-2019 academic years. The population included 66 students consisting of three classes; one class (I.2) was taken as a sample by using cluster sampling, as many as 22 students. The instrument used in this research was reading test. The result of this research showed that the pre-test got the mean score of 44.50, while the post-test 64.18. It showed that the p-value (0.000) was lower than the level of significance (alpha (α) = 0.05). It means that H1 was accepted. The research concluded that teaching reading comprehension by using a constructivist approach enhances students’ reading comprehension at the First year of UMS Rappang.


Rangifer ◽  
1990 ◽  
Vol 10 (3) ◽  
pp. 55 ◽  
Author(s):  
K. Rissanen ◽  
T. Rahola

After the Chernobyl accident the sampling and measuring program of the Finnish Centre for Radiation and Nuclear Safety was intensified both for surveillance and research purposes. The deposition pattern of radionuclides was more complicated than from the global fallout after the nuclear weapons tests. The radioactive deposition was very unevenly distributed in Lapland, as also in the rest of Finland. Fortunately, the amounts of deposition in Lapland were only about one-tenth of the corresponding amount of deposition in southern Finland. In 1986-87 the mean concentration of Cs-137 in lichens and in reindeer meat increased to about the same level as in 1972-73 or to about 30 per cent of the maximum levels found in 1964-65 after the nuclear weapons tests. The activity concentrations in reindeer tissues vary according to season. In winter, reindeer eat considerable amounts of lichens with high radiocesium concentrations. In summer, lichens are replaced by other forage such as leaves from trees, green plants, etc. The ratio of Cs-137 concentration in reindeer meat between summer and winter is about 0.2. The mean concentration of Cs-137 in meat for consumption from the slaughtering period 1986-87 was 720 Bq/kg fresh weight. After that time concentrations started decreasing since no new fallout was deposited.


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