High-Definition Video Recording: Taking Sport Technology to the Operating Room

2018 ◽  
Vol 66 (08) ◽  
pp. 667-669 ◽  
Author(s):  
C. de Sierra ◽  
J. Cuenca-Castillo ◽  
F. Estevez-Cid
Keyword(s):  
Video Recording ◽  
Digital Recording ◽  
High Definition ◽  
High Quality ◽  
Video Sharing ◽  
Long Term Storage ◽  
Digital Contents ◽  
Economical System ◽  
Term Storage

AbstractFilming surgeries for teaching purposes, publications, and patient records has become increasingly popular as the systems for digital recording have evolved, becoming high-quality systems, both smaller and lighter. Digital recording allows long-term storage, retrieval, and database organization. In addition, sharing digital contents has also become easier since video sharing sites and social networks make it possible to upload these contents onto the Internet. We describe a simple and economical system for surgeons to record surgeries in high definition under sterile conditions without any interference with the surgeon's line of vision.

scholarly journals Unconventional effects of long-term storage of microwave-modified chicken egg white lysozyme preparations

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Grzegorz Leśnierowski ◽  
Tianyu Yang ◽  
Renata Cegielska-Radziejewska
Keyword(s):  
Microwave Radiation ◽  
Egg White ◽  
Process Time ◽  
High Quality ◽  
Chicken Egg ◽  
Egg White Lysozyme ◽  
Physicochemical Changes ◽  
Long Term Storage ◽  
Term Storage

AbstractThermal modification is an effective method that induces significant expansion of the antimicrobial properties and other valuable properties of chicken egg white lysozyme. In our latest research, a new innovative method of enzyme modification was developed, in which microwave radiation was used as an energy source to process liquid lysozyme concentrate (LLC). After modification, high-quality preparations were obtained. However, long-term storage in a concentrated form initiated various processes that caused darkening over time and could also lead to other significant changes to their structure and, consequently, to their functional properties. This necessitated multidirectional research to explain this phenomenon. This paper presents the results of research aimed at assessing the physicochemical changes in the properties of microwave-modified lysozyme in the form of a liquid concentrate after long-term storage under refrigeration conditions. The assessment also considered the conditions under the acidity of the modifying medium and the duration of the microwave modification. The analysis showed that the values of the basic parameters determining the quality and usefulness of the modified enzyme significantly improved during long-term storage of the preparations. The greatest changes were observed in the preparations modified for the longest time and in the most acidic environment (process time 260 s, pH 2.0), the number of oligomers under these conditions increased by 18% after 12 months of holding, and the surface hydrophobicity increased by as much as 31%. In addition, microbiological tests showed that the preparations of microwave-modified lysozyme had an effect on gram-positive bacteria as well as on gram-negative, and this effect was significantly enhanced after 12 months. The results confirm that LLC modification with microwave radiation is a highly efficient method to prepare high-quality and high utility potential lysozyme. Notably, an interesting and important phenomenon was the observation of the unconventional behaviour of the preparations during their long-term storage, which increased their utility potential significantly.

Summer Pruning: An Ecological Alternative to Postharvest Calcium Treatment to Improve Storability of High Quality Apple cv. ‘Reinette du Canada’

2010 ◽  
Vol 16 (4) ◽  
pp. 343-350 ◽  
Author(s):  
M. Guerra ◽  
P.A. Casquero
Keyword(s):  
Titratable Acidity ◽  
Soluble Solids ◽  
High Quality ◽  
Long Term Storage ◽  
Postharvest Application ◽  
Bitter Pit ◽  
Best Parameter ◽  
Term Storage ◽  
Organic Orchards

Two strategies, summer pruning and postharvest Ca treatment, were studied in apple (Malus domestica Borkh) cv. ‘Reinette du Canada’ in order to analyze its effect on the fruit quality during storage. Summer pruning and Ca treatment reduced external and internal bitter-pits; so after 180 days of storage, both treatments decreased external bitter-pit by 10.0% and 16.7%, respectively. Summer pruning influenced color, firmness, total soluble solids and titratable acidity (TA) of fruit during storage, whereas Ca treatment only affected firmness and TA. Fruit from pruned trees had significant lower K and Mg than those from unpruned trees and Ca treatment increased Ca content. Orchard management, by means of summer pruning, combined with Ca postharvest application would be useful to prevent losses due to bitter-pit during storage in commercial orchards. However, in organic orchards, summer pruning would be the ecological alternative to decrease bitter-pit incidence during storage in high quality apple cv. ‘Reinette du Canada’. K/Ca ratio, on the peel at harvest, turned out to be the best parameter to correlate with external and internal bitter-pits during storage; so this ratio would be useful to predict bitter-pit on long-term storage.

scholarly journals Rapid, Reproducible, Quantifiable NMR Metabolomics: Methanol and Methanol: Chloroform Precipitation for Removal of Macromolecules in Serum and Whole Blood

Metabolites ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 93 ◽  
Author(s):  
Cora McHugh ◽  
Thomas Flott ◽  
Casey Schooff ◽  
Zyad Smiley ◽  
Michael Puskarich ◽  
...  
Keyword(s):  
Human Serum ◽  
1H Nmr ◽  
Whole Blood ◽  
Nmr Spectra ◽  
High Quality ◽  
Healthy Human ◽  
Long Term Storage ◽  
The Impact ◽  
Term Storage

Background: Though blood is an excellent biofluid for metabolomics, proteins and lipids present in blood can interfere with 1d-1H NMR spectra and disrupt quantification of metabolites. Here, we present effective macromolecule removal strategies for serum and whole blood (WB) samples. Methods: A variety of macromolecule removal strategies were compared in both WB and serum, along with tests of ultrafiltration alone and in combination with precipitation methods. Results: In healthy human serum, methanol:chloroform:water extraction with ultrafiltration was compared to methanol precipitation with and without ultrafiltration. Methods were tested in healthy pooled human serum, and in serum from patients with sepsis. Effects of long-term storage at −80 °C were tested to explore the impact of macromolecule removal strategy on serum from different conditions. In WB a variety of extraction strategies were tested in two types of WB (from pigs and baboons) to examine the impact of macromolecule removal strategies on different samples. Conclusions: In healthy human serum methanol precipitation of serum with ultrafiltration was superior, but was similar in recovery and variance to methanol:chloroform:water extraction with ultrafiltration in pooled serum from patients with sepsis. In WB, high quality, quantifiable spectra were obtained with the use of a methanol: chloroform precipitation.

Understanding Actinides through the Role of 5f Electrons

MRS Bulletin ◽  
2001 ◽  
Vol 26 (9) ◽  
pp. 684-688 ◽  
Author(s):  
T. Gouder ◽  
F. Wastin ◽  
J. Rebizant ◽  
G.H. Lander
Keyword(s):  
Predictive Power ◽  
Quality Data ◽  
Nuclear Fuels ◽  
High Quality ◽  
High Quality Data ◽  
Long Term Storage ◽  
5F Electrons ◽  
Term Storage

Studies of the actinide elements and compounds were (and are) motivated by the need to characterize their structural and thermodynamic properties for the development of nuclear fuels and the treatment of waste, whether it be for long-term storage or ideas involving transmutation in high-powered accelerators. For the most part, tables giving these data exist, although the data for transuranium compounds are rather sparse. A much more difficult task is to understand the data and develop theories that have predictive power in this part of the periodic table. In doing this, however, we are confronted with the extremely complicated electronic structure of the 5f shell and the great paucity of high-quality data on materials containing transuranium isotopes.

scholarly journals FTA Cards for Preservation of Nucleic Acids for Molecular Assays: A Review on the Use of Cytologic/Tissue Samples

2018 ◽  
Vol 142 (3) ◽  
pp. 308-312 ◽  
Author(s):  
Gilda da Cunha Santos
Keyword(s):  
Nucleic Acids ◽  
Molecular Techniques ◽  
Long Distance ◽  
High Quality ◽  
Molecular Assays ◽  
Fta Cards ◽  
Long Term Storage ◽  
Term Storage

Context.— Traditional methods for storing histologic and cytologic specimens for future use in molecular assays have consisted of either snap-freezing with cryopreservation or formalin-fixing, paraffin-embedding the samples. Although snap-freezing with cryopreservation is recommended for better preservation of nucleic acids, the infrastructure and space required for archiving impose challenges for high-volume pathology laboratories. Cost-effective, long-term storage at room temperature; relatively easy shipment; and standardized handling can be achieved with formalin-fixed, paraffin-embedded samples, but formalin fixation induces fragmentation and chemical modification of nucleic acids. Advances in next-generation sequencing platforms, coupled with an increase in diagnostic, prognostic, and predictive molecular biomarkers have created a demand for high-quality nucleic acids. To address issues of the quality of nucleic acid and logistics in sample acquisition, alternatives for specimen preservation and long-term storage have been described and include novel universal tissue fixatives, stabilizers, and technologies. Objective.— To collect, retrieve, and review information from studies describing the use of nucleic acids recovered from cytologic/tissue specimens stored on Flinders Technology Associates (FTA, GE Whatman, Maidstone, Kent, United Kingdom) cards for downstream molecular applications. Data Sources.— An electronic literature search in the PubMed (National Center for Biotechnology Information, Bethesda, Maryland) database allowed the selection of manuscripts addressing the use of FTA cards for storage of cytologic samples for molecular analysis. Only articles published in English were retrieved. Conclusions.— The use of FTA cards is a versatile method for fostering multicenter, international collaborations and clinical trials that require centralized testing, long-distance shipment, and high-quality nucleic acids for molecular techniques. Studies with controlled temperature are required to test the quality of recovered RNA after long-term storage.

scholarly journals Influence of Frozen Storage Duration and Forcing Temperature on Flowering of Oriental Hybrid Lilies

HortScience ◽  
2001 ◽  
Vol 36 (6) ◽  
pp. 1053-1056 ◽  
Author(s):  
Jong Suk Lee ◽  
Mark S. Roh
Keyword(s):  
Air Conditioning ◽  
Floral Development ◽  
Frozen Storage ◽  
High Quality ◽  
Storage Duration ◽  
Long Term Storage ◽  
Term Storage ◽  
Oriental Hybrid Lilies ◽  
Scanning Electron

The effect of long-term storage of lily bulbs at -2 °C (frozen storage) and of high forcing temperatures on plant height and floral abnormalities was investigated with Oriental hybrid lilies from 1998 to 2000. `Acapulco' and `Simplon' bulbs were stored frozen at -2 °C for various lengths of time and were forced in fan- and pad-cooled greenhouses with temperatures ranging from 11 to 31 °C, depending on the season. The same cultivars were also forced in greenhouses and maintained year-round under refrigerated air conditioning with day/night temperatures of 16/15.5 °C or 18.5/18 °C. Floral development immediately after storage and at different intervals thereafter was observed by scanning electron microscopy (SEM). The prolonged frozen storage reduced the number of flowers. High greenhouse forcing temperatures during summer significantly accelerated flowering, resulted in short plants, and increased the number of abnormal flowers. Forcing at a low temperature (15.5 °C) after planting the frozen stored bulbs resulted in longer cut stems than those forced at 25 °C for 30 days after potting. Bulbs can be stored up to 9 months and still produce high-quality Oriental hybrid lilies.

scholarly journals A Tissue Retrieval and Postharvest Processing Regimen for Rodent Reproductive Tissues Compatible with Long-Term Storage on the International Space Station and Postflight Biospecimen Sharing Program

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
Vijayalaxmi Gupta ◽  
Lesya Holets-Bondar ◽  
Katherine F. Roby ◽  
George Enders ◽  
Joseph S. Tash
Keyword(s):  
Posttranslational Modifications ◽  
Space Station ◽  
Tissue Storage ◽  
High Quality ◽  
International Space ◽  
Reproductive Tissues ◽  
Long Term Storage ◽  
Species Specific ◽  
Term Storage

Collection and processing of tissues to preserve space flight effects from animals after return to Earth is challenging. Specimens must be harvested with minimal time after landing to minimize postflight readaptation alterations in protein expression/translation, posttranslational modifications, and expression, as well as changes in gene expression and tissue histological degradation after euthanasia. We report the development of a widely applicable strategy for determining the window of optimal species-specific and tissue-specific posteuthanasia harvest that can be utilized to integrate into multi-investigator Biospecimen Sharing Programs. We also determined methods for ISS-compatible long-term tissue storage (10 months at −80°C) that yield recovery of high quality mRNA and protein for western analysis after sample return. Our focus was reproductive tissues. The time following euthanasia where tissues could be collected and histological integrity was maintained varied with tissue and species ranging between 1 and 3 hours. RNA quality was preserved in key reproductive tissues fixed in RNAlaterup to 40 min after euthanasia. Postfixation processing was also standardized for safe shipment back to our laboratory. Our strategy can be adapted for other tissues under NASA’s Biospecimen Sharing Program or similar multi-investigator tissue sharing opportunities.

A Practical, Large-Scale Synthesis of p-(Difluoroiodo)toluene (p-TolIF2)

Synthesis ◽  
2019 ◽  
Vol 51 (16) ◽  
pp. 3055-3059 ◽  
Author(s):  
Jason Tao ◽  
Graham K. Murphy
Keyword(s):  
Large Scale ◽  
High Quality ◽  
Elemental Fluorine ◽  
Fluorinating Agent ◽  
Long Term Storage ◽  
Quality Product ◽  
Large Scale Synthesis ◽  
Term Storage ◽  
High Quality Product

p-(Difluoroiodo)toluene (p-TolIF2) is a versatile fluorinating agent that acts as both a surrogate for elemental fluorine, and as a source of ‘electrophilic’ fluorine. Described here is a detailed three-step synthesis of p-TolIF2, carried out on a 50 mmol scale, that consistently provides high-quality product that is suitable for long-term storage. The reactions employ inexpensive, readily available starting materials and reagents, and uses the commodity chemical 48% aqueous HF as the source of fluorine atoms.

Studies of long-term storage of high quality raw sugar

2010 ◽  
pp. 419-426
Author(s):  
Vadim Kochergin
Keyword(s):  
Sugar Content ◽  
Storage Period ◽  
Future Research ◽  
High Quality ◽  
The Core ◽  
Long Term Storage ◽  
Short Period ◽  
Raw Sugar ◽  
Term Storage

Although existing contracts do not provide sufficient economic justification to increase raw sugar quality, the trend of manufacturing better quality raw sugar in the factories will likely continue. Relatively few studies have been conducted on storage of VHP (very high pol) and VLC (very low color) sugars, especially when the storage period approaches 9–10 months. Monitoring of commercial sugar shipments indicated that, after an initial relatively safe period of storage, sugar color might double or even triple in a short period of time. Large experimental piles of VHP and VLC sugar were monitored in two sugar factories with different crystallization sequences (conventional and double magma) for two consecutive seasons. Temperature and relative humidity probes were placed up to 15 m inside the piles. Samples were taken periodically to evaluate the effects of storage conditions on color, purity, invert sugar content and other parameters. It was concluded that sugar of high quality stores better compared to conventional sugar. However, even VHP sugar can double its color during long-term storage. Sampling near the surface of the sugar pile (up to 1.5 m inside the pile) is not representative of the bulk of sugar. It has been found that sugar temperature follows the ambient trend as deep as 3 m inside the pile. Sugar within 1.5 m of the surface that was not subjected to temperature increase stored well compared to sugar in the core of the pile. It is unclear what conditions trigger color increase in storage. Changes in temperature profiles of raw sugar during storage in commercial warehouses indicate that some exothermic reactions take place in the core of the piles that result in color increase and reduction of sucrose content. The reactions take place even when the initial sugar water content and temperature meet the requirements accepted for safe storage (safety factor below 0.25 and temperature below 30 °C). Lower sugar pH value may be one of the reasons that make sugar less stable in storage. Options of cooling sugar before or during the storage will be considered in future research.

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