scholarly journals Peroxidase Activity, Isoenzymes, and Tissue Localization in Developing Highbush Blueberry Fruit

1991 ◽  
Vol 116 (5) ◽  
pp. 827-830 ◽  
Author(s):  
T.J. Miesle ◽  
A. Proctor ◽  
L.M. Lagrimini

The activity, isoenzymes, and histochemical location of peroxidase were studied in developing highbush blueberries (Vaccinium corymbosum L.). Total peroxidase activity increased during development when expressed on a fresh-weight basis, reaching a maximum in red berries and then declining. When peroxidase activity was expressed per berry it did not decline after the red-berry stage. Most of the enzyme was ionically bound to cell walls throughout development, with the number of isoenzymes increasing with maturity. Histochemical localization of peroxidase showed that enzyme activity spread throughout the berry during development.

1999 ◽  
Vol 79 (4) ◽  
pp. 617-623 ◽  
Author(s):  
W. Kalt ◽  
J. E. McDonald ◽  
R. D. Ricker ◽  
X. Lu

Anthocyanins in ripe fruit of four Vaccinium species and genotypes within these species were compared, revealing substantial inter- and intra-species variability among these commercial and non-commercial blueberries. The highest total anthocyanin content occurred in the bilberry (V. myrtillus L.). Commercial lowbush blueberry clonal mixtures and wild velvet leaf blueberries (V. myrtilloides L.) had about 43% of the anthocyanin content of bilberries (fresh weight basis). Three commercial highbush cultivars (V. corymbosum L.) had about 30% of the bilberry level, while wild genotypes of highbush blueberries had almost 60% of the bilberry level. Acetylation of anthocyanins occurred widely among all species, except bilberries. Although the proportions of the five blueberry anthocyanidins varied substantially among the commercial blueberries, these differences probably do not contribute substantially to differences in their relative antioxidant capacity. Key words: Vaccinium, bilberry, acetylation, cluster analysis, antioxidant


Author(s):  
Pinkal Patel ◽  
Ratna Trivedi

The Milky mushroom, Calocybe Indica was cultivated on different agricultural substrate, paddy straw, wheat straw, sugarcane trace and mango dry leaves. The spawning was done by sterilization of all the four substrate. The bags were kept in mushroom growing room with the maintenance of temperature and humidity 30̊ c-35̊ c and 70-80 % respectively. The minimum days requires for completion of spawn run (18.4 days), primordial formation (25.2 days) and days for first harvest (32.4 days) was first observed on cultivation with Paddy straw.  The maximum yield on fresh weight basis and biological efficiency (134.86 %) was also found to be as the same treatment with the Paddy straw as a substrate. The biological efficiency of wheat straw was at par with Sugarcane trace as substrate which was 85.07 % and 85.02 % respectively.


1968 ◽  
Vol 46 (7) ◽  
pp. 921-928 ◽  
Author(s):  
D. J. Durzan

In late August during the onset of dormancy in spruce, seasonal levels of soluble nitrogen, rich in arginine, were high. On a fresh weight basis, diurnal levels of total soluble nitrogen and most component amino acids in roots, buds, and leaves showed maxima, one at sunrise and another in the afternoon or near sunset.Arginine and glutamine in the different plant parts contributed 44 to 83% to the alcohol-soluble nitrogen. In buds and leaves, percentage of arginine remained high and decreased slightly at midday, whereas in roots a continual drop occurred. In all organs examined, changes in glutamine reflected the double maxima of total soluble nitrogen and were greatest in roots.On a fresh weight basis, most amino acids accumulated at sunrise and near sunset; however a few especially in leaves, increased at midday, e.g. glutamic and aspartic acid, lysine, γ-aminobutyric acid, and serine.Comparison of levels of free guanidino compounds in different organs showed remarkable out-of-phase patterns. Levels of these compounds are known from 14C-arginine studies to be closely related to the metabolism of arginine.


1999 ◽  
Vol 77 (3) ◽  
pp. 323-332
Author(s):  
Suzy Y Rogiers ◽  
N Richard Knowles

Changes in respiration and ethylene production rates of nine maturity classes of saskatoon (Amelanchier alnifolia, Nutt.) fruit after harvest were compared with those of fruit maturing and ripening on the plant. During ripening on the plant, ethylene production increased on a whole-fruit and fresh-weight basis, while respiration increased substantially on a whole-fruit basis but remained constant on a fresh-weight basis. Fruit harvested at the greenish yellow to pink stages (maturity classes four and five, respectively) increased their ethylene production until 15-20 h after harvest, and this was coincident with ripening to maturity class seven and thus a color change to red. Ethylene production then declined over the next 15 h. Respiration rates of harvested immature, mature, and ripe fruits declined over 5 days, except for a relatively brief 8-12% rise starting 15-20 h after harvest. This brief increase in respiration was characteristic of fruit of all maturity classes, likely reflecting a wound response associated with harvest rather than a ripening-induced change in metabolism. Continuous treatment of attached or detached fruit with propylene or ethylene in an open airflow system did not induce an increase in respiration earlier, relative to control fruit. The respiratory response of saskatoon fruit during postharvest ripening was thus very different from that of fruit that ripened on the plant. Thus, the increase in respiration associated with ripening could only be demonstrated on a whole-fruit basis if fruit remained on the plant. Preharvest and postharvest changes in ethylene production during ripening also differed but were both consistent with climacteric ripening.Key words: Amelanchier alnifolia, ethylene, ripening, saskatoon fruit.


1970 ◽  
Vol 43 (4) ◽  
pp. 483-494 ◽  
Author(s):  
M Moshfekus Saleh-e-in ◽  
Abida Sultana ◽  
M Abul Hossain ◽  
Mainul Ahsan ◽  
Sudhangshu Kumar Roy

Anethum sowa L. (Dill) stem portions were analyzed for its macro and micro elemental concentration by XRF spectrometry collected from different places of Bangladesh. The plants were collected when those were 35 cm in height i.e. within 40-45 days of sowing. Results of proximate analyses showed that the stem parts contained highest level of moisture (93.67), ash (1.62) and dry matter (30.77%) on fresh weight basis (g/100g). The highest level of mineral contents were Ca(478.50±6.22), K(336.65±3.26), Cl(153.92±3.26), Na(114.86±1.38), S(96.65±1.76), Mg(63.96±1.15), P(49.41±0.74), Al(41.75±0.71), Fe(34.57±1.33), Si(31.03±0.18), Ni (11.98±0.22) and Ti (3.75±0.28) in mg/100g on fresh weight basis. Cu, Sr, Rb, Zn, Ba and Zr were present in small amount and Y, Cu and Mn were present in trace amount. However, the toxic elements like Co, Pb, As and Hg were not found in the present study. Presence of higher amounts of mineral contents in the stem parts could potentially be consumed as supplement as human diets or livestock feed as well as pharmaceutical preparation. Key words: Anethum sowa L., XRF spectrometry, Dill, Condiment, Macro and micro elements, Elemental composition.     doi: 10.3329/bjsir.v43i4.2238   Bangladesh J. Sci. Ind. Res. 43(4), 483-494, 2008


1981 ◽  
Vol 59 (8) ◽  
pp. 884-886 ◽  
Author(s):  
Adrien R. Beaudoin ◽  
Pierre St-Jean ◽  
Ginette Girard

Recent observations have confirmed the existence of more than one pool of secretory proteins in the rat pancreatic tissue. To determine if these different pools could be located in the different regions of the rat pancreas, the amylase and chymotrypsin contents have been measured in the biliary, duodenal, gastric, and splenic regions. On a tissue DNA content, protein content, or a fresh weight basis, the proportions of these two enzymes are comparable in the four regions. It is therefore postulated that heterogeneity of enzyme composition exists either within these regions or within the acinar cell itself.


2012 ◽  
Vol 58 (No. 5) ◽  
pp. 236-241 ◽  
Author(s):  
S. Mihaljević ◽  
B. Salopek-Sondi

Auxins and their synthetic analogues are commonly used for rooting of cuttings, but their efficiency depends on experimental set-up and, even more importantly, on species or cultivar, and type of explants investigated. In attempt to improve rooting procedure for highbush blueberries (Vaccinium corymbosum L.), we investigated alanine conjugate of indole-3-butyric acid (IBA-Ala) as potential root-promoting compound and compared with commonly used auxins indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA). The effect of different concentrations of auxins on the rooting of highbush blueberry stem cuttings (var. Bluecrop, Bluetta, Burlington and Jersey) and in vitro-derived microcuttings (var. Jersey) was investigated. Auxin treatments significantly promoted rooting of all four varieties in comparison to control. The most efficient rooting promoter in all varieties appeared IBA-Ala (provided up to 83%, and 93% of the rooted cuttings and microcuttings, respectively). Furthermore, IBA-Ala caused a vigorous, well-branched root system that resulted in better acclimatization and survival of plants. Results suggest the application of IBA-Ala may be beneficial for rooting of difficult-to-root varieties of blueberry and related species and their practical application in the nursery industry.  


2011 ◽  
Vol 29 (11) ◽  
pp. 1164-1170 ◽  
Author(s):  
Jonathan W. C. Wong ◽  
Ammaiyappan Selvam ◽  
Zhenyong Zhao ◽  
S. M. Yu ◽  
Alex C. W. Law ◽  
...  

Composting sewage sludge alone would reduce the decomposition efficiency due to free limited porosity in sludge. To alleviate this, the use of horse stable straw bedding waste (HSB) was evaluated as a co-composting material with sewage sludge in a 10 tonnes day−1 in-vessel composter for a period of 7 days before curing in a static aeration pile. Sludge was mixed with HSB at 1 : 1.5 (HSL) and 1 : 2.9 (LSL) on a fresh weight basis. After a composting period of 56 days, both mixing ratios demonstrated to be feasible with LSL having a better organic decomposition and a shorter time to reach maturity. The overall decomposition rates were 52.0 and 58.9% (dry weight basis) for HSL and LSL, respectively. In both treatments, temperature in the in-vessel composters could reach 65°C, which was sufficient to remove the pathogens. Although both products were free of pathogens, HSL exhibited a higher ammoniacal nitrogen contents but a lower seed germination index than that of LSL indicating a higher phytotoxicity and a longer curing period would be required. It can be concluded that HSB provided a better composting conditions at a mixing ratio of 1 : 2.9


2016 ◽  
Author(s):  
Gerald R. Stephenson ◽  
Ian G. Ferris ◽  
Patrick T. Holland ◽  
Monica Nordberg

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