scholarly journals Diaskintest for the diagnosis of bovine tuberculosis

2021 ◽  
Vol 98 (12) ◽  
pp. 53-56
Author(s):  
A. Kh. Naymanov ◽  
A. M. Gulyukin ◽  
N. G. Tolstenko ◽  
E. P. Vangeli ◽  
V. M. Kalmykov
Keyword(s):  
Differential Diagnosis ◽  
Adverse Events ◽  
Bovine Tuberculosis ◽  
Guinea Pigs ◽  
Tuberculin Test ◽  
Diagnostic Value ◽  
Farm Animals ◽  
Laboratory Animals

The objective of the study: to compare the diagnostic value of intracutaneous tests with tuberculin and diaskintest for detection of tuberculosis in cattle.Subjects and methods. In this study, PPD tuberculin for mammals was used, it was made from M. bovis strain AN-5, manufactured by BIOK, and Diaskintest (recombinant tuberculous allergen, solution forintracutaneous administration) manufactured by ZAO FF Lecco, series 030307.Laboratory animals (guinea pigs, rabbits, chickens) and farm animals (pigs and cattle) were used in the study.Results. It has been established on laboratory animals that diaskintest does not cause any adverse events. When assessing sows sensitized with M. avium, it was found that the intracutaneous administration of PPD tuberculin to mammals resulted in up to 8.5% of positive reactions, while diaskintest was negative in all of them. Among cattle sensitized to non-tuberculous mycobacteria, intracutaneous administration of tuberculin for mammals revealed up to 4.6% of positive reactions, and diaskintest – up to 0.6%. In the farm with the unfavorable TB situation where animals infected with M. bovis were previously detected, when examining 177 cows, tuberculin test was positive in 102 (57.6%) of them, and diaskintest in 88 (49.7%). Diaskintest can be used for differential diagnosis of tuberculosis and sensitization by non-tuberculous mycobacteria in pigs and cattle.

scholarly journals Effects of microbicide based on lactic acid and metal nanoparticles on laboratory animals

2017 ◽  
Vol 7 (4) ◽  
pp. 482-485 ◽  
Author(s):  
G. V. Ponomarenko ◽  
V. L. Kovalenko ◽  
O. V. Ponomarenko ◽  
Yu. O. Balackiy
Keyword(s):  
Lactic Acid ◽  
Acute Toxicity ◽  
Metal Nanoparticles ◽  
Guinea Pigs ◽  
The Body ◽  
Farm Animals ◽  
Laboratory Animals ◽  
Harmful Effects ◽  
Nanoparticles Of Silver

<p>Modern complex disinfectants should not only be highly effective against most pathogens, but also safe for the body of laboratory and farm animals. To determine the effect of microbicides on animal organism, there are several commonly accepted parameters that are regulated by relevant methodological documents. Taking into account the urgency of the development and implementation of modern disinfectants, the purpose of the researche was to study the effects of the drug "Orgsept" on the indicators of acute toxicity and its harmful effects on laboratory animals. The determination of acute toxicity, the study of the cumulative and skin-resorptive action of the drug "Orgasept" was performed on clinically healthy white mice, and the irritating effect and sensitizing properties were studied on Guinea pigs. The studies were conducted using generally accepted techniques (Yakubchak et al., 2005). According to the results of the research, acute toxicity, cumulative, sensitizing, irritating, skin-resorptive action of the disinfectant based on lactic acid and nanoparticles of silver "Orgasept" on laboratory animals were determined. It has been determined that the investigated preparation according to sanitary-and-hygienic norms GOST 12.1.007-76 belongs to the 3 groups of toxicity in concentrations which are significantly higher than bactericidal, it does not have a pronounced cumulative, sensitizing and skin-resorptive action.</p>

scholarly journals Studying of S- and L- form Brucella’s Thermo-Extracts Immunogenic Characteristics at Cavies Guinea Pigs

2016 ◽  
Vol 15 (4) ◽  
pp. 82-86 ◽  
Author(s):  
L. M. Mikhailov ◽  
N. L. Barannikova ◽  
L. E. Tokareva ◽  
S. A. Vityazeva ◽  
T. P. Starovoytova ◽  
...  
Keyword(s):  
Guinea Pigs ◽  
Small Ruminants ◽  
Farm Animals ◽  
Laboratory Animals ◽  
Important Species ◽  
Post Vaccination ◽  
Epizootic Process ◽  
Series Of Experiments ◽  
The Russian Federation ◽  
High Doses

Relevance. In the Russian Federation is noted the negative dynamics of epizootic process of brucellosis among epidemiologically important species of farm animals (cattle and small ruminants), which represents a threat to the population. Used in Russia live vaccine based on a strain of Brucella abortus 19 the BA has reduced virulence, but capable at high doses (108 -2 109 m.c.) cause a generalized infection in guinea pigs and humans, and in violation of the rules cause post-vaccination complications. Goal. Assess possibility of the thermo-extract derived from the S- and L-forms of Brucella, get an immune response in guinea pigs, and reduce the risk of infection with virulent brucella. Materials and methods. Two series of experiments were carried out on guinea pigs. Immunized guinea pigs thermo-extracts (TE) from a strain of B. abortus I-206 in the S- and L-forms, and live brucellosis vaccine (Scientific and Production Association for Immunological Preparations «Microgen», Russia). To infect guinea pigs using virulent B. abortus 544 (Reference) and B. melitensis I-203 from the museum of living cultures of the Irkutsk Scientific Research Anti-Plague Institute. Results. In the first and second experiment after immunization L TE in dose 5 mg and 10 mg after infection with B. abortus and B. melitensis 5441-203 were approximately similar results. Immunization of Brucella in TE S-form or complex of L + S TE either of two doses (5 mg or 10 mg) cultures after infection B. abortus and B. melitensis 5441-203 gave the same result as a vaccine B. abortus 19VA. Conclusions. The results indicate the prospects of further study of experimental steps for using immunizing agents TE S, L TE and TE S + L on the laboratory animals.

scholarly journals THE PATHOLOGIC EFFECTS OF STREPTOCOCCI FROM CASES OF POLIOMYELITIS AND OTHER SOURCES

1917 ◽  
Vol 25 (4) ◽  
pp. 557-580 ◽  
Author(s):  
Carroll G. Bull
Keyword(s):  
Spinal Cord ◽  
Guinea Pigs ◽  
The Other ◽  
Laboratory Animals ◽  
Histological Changes ◽  
Other Hand ◽  
Brain And Spinal Cord ◽  
The Brain

Streptococci cultivated from the tonsils of thirty-two cases of poliomyelitis were used to inoculate various laboratory animals. In no case was a condition induced resembling poliomyelitis clinically or pathologically in guinea pigs, dogs, cats, rabbits, or monkeys. On the other hand, a considerable percentage of the rabbits and a smaller percentage of some of the other animals developed lesions due to streptococci. These lesions consisted of meningitis, meningo-encephalitis, abscess of the brain, arthritis, tenosynovitis, myositis, abscess of the kidney, endocarditis, pericarditis, and neuritis. No distinction in the character or frequency of the lesions could be determined between the streptococci derived from poliomyelitic patients and from other sources. Streptococci isolated from the poliomyelitic brain and spinal cord of monkeys which succumbed to inoculation with the filtered virus failed to induce in monkeys any paralysis or the characteristic histological changes of poliomyelitis. These streptococci are regarded as secondary bacterial invaders of the nervous organs. Monkeys which have recovered from infection with streptococci derived from cases of poliomyelitis are not protected from infection with the filtered virus, and their blood does not neutralize the filtered virus in vitro. We have failed to detect any etiologic or pathologic relationship between streptococci and epidemic poliomyelitis in man or true experimental poliomyelitis in the monkey.

scholarly journals Diagnostic value of diffusion weighted MRI and ADC in differential diagnosis of cavernous hemangioma of the liver

2016 ◽  
Vol 16 (1) ◽  
pp. 227 ◽  
Author(s):  
Ozlem Tokgoz ◽  
Ebru Unlu ◽  
Ilker Unal ◽  
Ismail Serifoglu ◽  
Ilker Oz ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Cavernous Hemangioma ◽  
Diagnostic Value ◽  
Diffusion Weighted Mri ◽  
Diffusion Weighted

scholarly journals Tremorography in the clinical practice

2020 ◽  
Vol 9 (4) ◽  
pp. 61-72
Author(s):  
T. G. Govorova ◽  
T. E. Popova ◽  
A. A. Tappakhov
Keyword(s):  
Differential Diagnosis ◽  
Clinical Practice ◽  
Movement Disorders ◽  
Clinical Characteristics ◽  
Objective Assessment ◽  
Diagnostic Value ◽  
Common Type ◽  
Routine Practice ◽  
Pathological Tremor

Tremor is the most common type of movement disorders. In practice this differential diagnosis of hyperkinesis is diagnosed clinically and the use of additional methods of objective assessment of tremor increases the accuracy of diagnosis. The use of paraclinical methods of objective assessment of tremor improves the accuracy of diagnosis. Comparison of the neurophysiological parameters of tremor with clinical characteristics has a high diagnostic value, which justifies its use in the routine practice of neurologists. The purpose of the review is to analysis basic electrophysiological characteristics of pathological tremor, as well as the presentation of the material of its own observation.

scholarly journals Usefulness of procalcitonin (PCT), C-reactive protein (CRP), and white blood cell (WBC) levels in the differential diagnosis of acute bacterial, viral, and mycoplasmal respiratory tract infections in children

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Yang Li ◽  
Lanfang Min ◽  
Xin Zhang
Keyword(s):  
Differential Diagnosis ◽  
Respiratory Tract ◽  
Bacterial Infection ◽  
Bacterial Infections ◽  
Respiratory Tract Infections ◽  
Respiratory Infections ◽  
Diagnostic Value ◽  
Significant Difference ◽  
Group A ◽  
Tract Infections

Abstract Background There is a lack of studies comparing PCT, CRP and WBC levels in the differential diagnosis of acute bacterial, viral, and mycoplasmal respiratory tract infections. It is necessary to explore the correlation between above markers and different types of ARTI. Methods 108 children with confirmed bacterial infection were regarded as group A, 116 children with virus infection were regarded as group B, and 122 children with mycoplasmal infection were regarded as group C. The levels of PCT, CRP and WBC of the three groups were detected and compared. Results The levels of PCT, CRP and WBC in group A were significantly higher than those in groups B and C (p < 0.05). The positive rate of combined detection of PCT, CRP and WBC was significant higher than that of single detection. There was no significant difference in PCT, CRP and WBC levels between the group of G+ bacterial infection and G− bacterial infection (p > 0.05). ROC curve results showed that the AUC of PCT, CRP and WBC for the diagnosis of bacterial respiratory infections were 0.65, 0.55, and 0.58, respectively. Conclusions PCT, CRP and WBC can be combined as effective indicators for the identification of acute bacterial or no-bacterial infections in children. The levels of PCT and CRP have higher differential diagnostic value than that of WBC in infection, and the combined examination of the three is more valuable in clinic.

scholarly journals Diagnostic value of magnetic resonance imaging in the analysis of ameloblastoma: report of two cases

2019 ◽  
Vol 22 (3) ◽  
pp. 425-431
Author(s):  
Neiandro Santos Galvão ◽  
Antonione Santos Bezerra Pinto ◽  
Alan Leandro Carvalho Farias ◽  
André Luiz Ferreira Costa ◽  
Sérgio Lúcio Pereira de Castro Lopes ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Differential Diagnosis ◽  
Magnetic Resonance ◽  
Signal Intensity ◽  
Diagnostic Value ◽  
Resonance Imaging ◽  
Intermediate Signal Intensity ◽  
Additional Information ◽  
Imaging Characteristics ◽  
Magnetic Resonance Imaging Mri

Ameloblastoma is an odontogenic tumor that shares clinical and imaging characteristics with other lesions of the jaws, such as odontogenic keratocyst, which makes the diagnosis difficult. However, in addition to radiographic and tomographic examinations, Magnetic Resonance Imaging (MRI) has been increasingly used, contributing with relevant additional information about the differentiation between solid and liquid components of the lesion. This case report was conducted to present two variations of ameloblastoma and discuss the radiographic, tomographic and MRI contribution in the differential diagnosis between ameloblastoma and odontogenic keratocyst.The signal intensity in T1-weighted MRI revealed internal fluid content in both cases, which was important in the differential diagnosis with other intraosseous lesions such as odontogenic keratocysts. This is probably due to the presence of keratin that increases the viscosity of the content and also for an intermediate signal intensity signal in T2-weighted MRI. Therefore, MRI revealed important internal characteristics of the reported lesions, which was very useful in the establishment of the differential diagnosis with other lesions.

scholarly journals Pediatric Appendicitis Score in differential diagnosis of acute nonspecific abdominal pain in children: age matters

2021 ◽  
pp. 22-31
Author(s):  
V.G. Vakulchyk ◽  
◽  
A.V. Kapytski ◽  
Keyword(s):  
Differential Diagnosis ◽  
Abdominal Pain ◽  
Acute Appendicitis ◽  
Roc Curves ◽  
Diagnostic Value ◽  
Diagnostic Significance ◽  
Predictive Values ◽  
Detection Frequency ◽  
Specificity And Sensitivity ◽  
Appendicitis Score

Acute nonspecific abdominal pain in children is the most common problem requiring differential diagnosis with acute appendicitis. Scales for integrated assessment of individual symptoms and their combinations have been proposed and are constantly being developed that allow predicting the likelihood of acute appendicitis. Purpose to assess diagnostic value of Pediatric Appendicitis Score (PAS) in groups of children in different ages. Materials and methods. 374 children aged 4 to 15 years with acute abdominal pain were evaluated in prospective randomized blinded study. Statistical analysis: ROC – curves, specificity and sensitivity, positive and negative predictive values; Kullback criteria; logistic regression analysis; discriminant analysis. Results. Detection frequency and diagnostic significance of the PAS scale predictors as well as obtained results by using the Pediatric Appendicitis Score depend on children age significantly. In terms of diagnosis of acute appendicitis, the PAS scale shows the best results in older children. Conclusions. Results of Pediatric Appendicitis Score depend on children ages due to different diagnostic value of predictors used in the PAS scale. Pediatric surgeons should keep in your mind these data. Modification of the scale is required taking into account the patient’s age. Further analysis of the issue of PAS using is needed. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of these Institutes. The informed consent of the patient was obtained for conducting the studies. The authors declare no conflicts of interests. Key words: acute appendicitis, children, diagnosis, PAS scale.

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