scholarly journals 3D-printable cell crowding device enables imaging of live cells in compression

BioTechniques ◽  
2020 ◽  
Vol 68 (5) ◽  
pp. 275-278
Author(s):  
Liam P Dow ◽  
Aimal H Khankhel ◽  
John Abram ◽  
Megan T Valentine

We designed and fabricated, using low-cost 3D printing technologies, a device that enables direct control of cell density in epithelial monolayers. The device operates by varying the tension of a silicone substrate upon which the cells are adhered. Multiple devices can be manufactured easily and placed in any standard incubator. This allows long-term culturing of cells on pretensioned substrates until the user decreases the tension, thereby inducing compressive forces in plane and subsequent instantaneous cell crowding. Moreover, the low-profile device is completely portable and can be mounted directly onto an inverted optical microscope. This enables visualization of the morphology and dynamics of living cells in stretched or compressed conditions using a wide range of high-resolution microscopy techniques.

2019 ◽  
Vol 12 (03) ◽  
pp. 1950014 ◽  
Author(s):  
Xibin Yang ◽  
Qian Zhu ◽  
Zhenglong Sun ◽  
Gang Wen ◽  
Xin Jin ◽  
...  

Structured illumination microscopy (SIM) is a promising super-resolution technique for imaging subcellular structures and dynamics due to its compatibility with most commonly used fluorescent labeling methods. Structured illumination can be obtained by either laser interference or projection of fringe patterns. Here, we proposed a fringe projector composed of a compact multi-wavelength LEDs module and a digital micromirror device (DMD) which can be directly attached to most commercial inverted fluorescent microscopes and update it into a SIM system. The effects of the period and duty cycle of fringe patterns on the modulation depth of the structured light field were studied. With the optimized fringe pattern, [Formula: see text] resolution improvement could be obtained with high-end oil objectives. Multicolor imaging and dynamics of subcellular organelles in live cells were also demonstrated. Our method provides a low-cost solution for SIM setup to expand its wide range of applications to most research labs in the field of life science and medicine.


2020 ◽  
Vol 4 (Supplement_1) ◽  
pp. 691-692
Author(s):  
Emily Ihara

Abstract Person-centered dementia care is a best practice recommendation by the Alzheimer’s Association, and non-pharmacologic interventions that emphasize well-being and quality of life as defined by the individual are important to preserve personhood. Non-pharmacologic, person-centered interventions have been shown to effectively address various neuropsychiatric symptoms, commonly known as behavioral and psychological symptoms in dementia (BPSD), which include a wide range of behaviors such as verbal or physical aggression, agitation, wandering, and pacing. Interventions that are focused on an individual’s holistic needs and preferences can stimulate positive emotions and behavior regardless of the stage of dementia. Person-centered care emphasizes a social model of care, rather than a medical model, by focusing on an individual’s emotional needs and care preferences that are consistent with their previous lifestyle. This symposium explores four different non-pharmacologic interventions for individuals living with dementia and discusses challenges and best practices for implementation in long-term care settings. For example, a best practice includes “buy-in” from facility staff who ultimately are responsible for implementing interventions that follow a social care model. A challenge found includes creating consistency and adherence to non-pharmacologic interventions so they are sustained over time, potentially replacing additional doses of medication. Symposium presenters will discuss the Mason Music & Memory Initiative (M3I), the Alzheimer’s Poetry Project, Birdsong, and TimeSlips, which are all interventions that are relatively low-cost and easy to implement by non-specialists. Strategies for intergenerational programming and adaptability of these programs to different contexts will also be discussed.


2003 ◽  
Vol 34 (10) ◽  
pp. 7-9 ◽  

Recent developments in micro electromechanical systems (MEMS) point towards a wide range of applications for a new breed of integrated wireless sensors. Applications include embedded sensors in “smart” buildings, for example vibration and temperature monitoring, medical implants and remote sensing. The new devices, which will communicate by microwatt power, short range radio (picoradio), have been compared with communicating neurons. The vision is that hundreds, or even thousands of small independent, low cost devices will be built into, say, a building, producing a powerful, interlinked network of information transmission. But they require a long- term power source, which can be a significant problem. The limitations of batteries have led to interest in scavenging by conversion of another form into electrical energy. Possible energy sources are solar, body functions such as breathing, natural temperature gradients etc. This article considers energy scavenging from vibration.


Author(s):  
Ahmed Fadl ◽  
Stefanie Demming ◽  
Zongqin Zhang ◽  
Stephanus Bu¨ttgenbach ◽  
Manfred Krafczyk ◽  
...  

The advantages of valve-less rectification micro pumps include having no moving parts, low cost, reliable, having the ability to pump particles-laden fluids and live cells, being compatible with a wide range of micro channel materials and working fluids. Most valve-less rectification micro pumps are based on passive rectifying geometries such as a nozzle/diffuser, Tesla (Valvular Conduit), and Bifurcation geometries. In this study, we present a new valve-less rectification minipump based on a dynamic rectifying geometry. The present work includes design, fabrication, and testing of the pump. The experimental results are presented in terms of flow rates and maximum back pressures.


Polymers ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 80
Author(s):  
Amruth C ◽  
Beata Luszczynska ◽  
Wassima Rekab ◽  
Marek Zdzislaw Szymanski ◽  
Jacek Ulanski

Among solution-processable techniques, inkjet printing is a potential method for manufacturing low-cost and high-resolution polymer organic light-emitting diodes (PLEDs) for displays/solid-state lighting applications. Herein, we demonstrate use of the inkjet printed cesium carbonate (Cs2CO3) film as an electron injection interlayer. We have elaborated the Cs2CO3 ink using an alcohol-based solvent for the industrial-grade printhead. The printed Cs2CO3 layer morphology was investigated by means of an optical microscope and an atomic force microscope. The PLEDs based on emissive polymer (Super Yellow) with printed Cs2CO3 interlayer show a remarkable current efficiency and luminance compared to the PLEDs made without the Cs2CO3 layer. Such results suggest that the Cs2CO3 is a promising material for the formulation of the electron injecting inkjet inks. The possibility of inkjet printing of an efficient electron injecting layer enables in situ patterning of PLEDs’ emission area. Such a simple and flexible technique can be applied for a wide range of applications such as signage, pictograms, advertising, smart packaging, etc.


Micromachines ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 1392
Author(s):  
Filippo Amadeo ◽  
Prithviraj Mukherjee ◽  
Hua Gao ◽  
Jian Zhou ◽  
Ian Papautsky

Fabrication of microfluidic devices by soft lithography is by far the most popular approach due to its simplicity and low cost. The approach relies on casting of elastomers, such as polydimethylsiloxane (PDMS), on masters fabricated from photoresists on silicon substrates. These masters, however, can be expensive, complicated to fabricate, and fragile. Here we describe an optimized replica molding approach to preserve the original masters by heat molding of polycarbonate (PC) sheets on PDMS molds. The process is faster and simpler than previously reported methods and does not result in a loss of resolution or aspect ratio for the features. The generated PC masters were used to successfully replicate a wide range of microfluidic devices, including rectangular channels with aspect ratios from 0.025 to 7.3, large area spiral channels, and micropost arrays with 5 µm spacing. Moreover, fabrication of rounded features, such as semi-spherical microwells, was possible and easy. Quantitative analysis of the replicated features showed variability of <2%. The approach is low cost, does not require cleanroom setting or hazardous chemicals, and is rapid and simple. The fabricated masters are rigid and survive numerous replication cycles. Moreover, damaged or missing masters can be easily replaced by reproduction from previously cast PDMS replicas. All of these advantages make the PC masters highly desirable for long-term preservation of soft lithography masters for microfluidic devices.


2002 ◽  
Vol 2 ◽  
pp. 1101-1107
Author(s):  
Sharon L. Huntley ◽  
Lawrence J. Ritchie ◽  
Steven J. Setford ◽  
Selwayan Saini

A major problem when dealing with environmental contamination is the early detection and subsequent surveillance of the contamination. This paper describes the potential of sub-surface sensor technology for the early detection of organic contaminants in contaminated soils, sediments, and landfill sites. Rugged, low-power hydrocarbon sensors have been developed, along with a data-logging system, for the early detection of phase hydrocarbons in soil. Through laboratory-based evaluation, the ability of this system to monitor organic contamination in water-based systems is being evaluated. When used in conjunction with specific immunoassays, this can provide a sensitive and low-cost solution for long-term monitoring and analysis, applicable to a wide range of field applications.


Author(s):  
Carl Malings ◽  
Rebecca Tanzer ◽  
Aliaksei Hauryliuk ◽  
Provat K. Saha ◽  
Allen L. Robinson ◽  
...  

2020 ◽  
pp. 66-73
Author(s):  
A. Simonova ◽  
S. Chudakov ◽  
R. Gorenkov ◽  
V. Egorov ◽  
A. Gostry ◽  
...  

The article summarizes the long-term experience of practical application of domestic breakthrough technologies of preventive personalized medicine for laboratory diagnostics of a wide range of socially significant non-infectious diseases. Conceptual approaches to the formation of an integrated program for early detection and prevention of civilization diseases based on these technologies are given. A vision of the prospects for the development of this area in domestic and foreign medicine has been formed.


Author(s):  
S.V. Borshch ◽  
◽  
R.M. Vil’fand ◽  
D.B. Kiktev ◽  
V.M. Khan ◽  
...  

The paper presents the summary and results of long-term and multi-faceted experience of international scientific and technical cooperation of Hydrometeorological Center of Russia in the field of hydrometeorology and environmental monitoring within the framework of WMO programs, which indicates its high efficiency in performing a wide range of works at a high scientific and technical level. Keywords: World Meteorological Organization, major WMO programs, representatives of Hydrometeorological Center of Russia in WMO


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