scholarly journals Differential regulation of inflammatory mediators and granule-associated mediators in neonatal sepsis observed in cord and peripheral blood

2013 ◽  
pp. 33
Author(s):  
Berla Thangam ◽  
Muthukumar
Keyword(s):  
Inflammatory Mediators ◽  
Peripheral Blood ◽  
Neonatal Sepsis ◽  
Differential Regulation

245 INFLAMMATORY MEDIATORS AS PREDICTORS OF NEONATAL SEPSIS.

2006 ◽  
Vol 54 (1) ◽  
pp. S122.1-S122
Author(s):  
M. Rowe ◽  
S. A. Snyder ◽  
C. R. Lyons ◽  
D. C. Alverson ◽  
P. Helman ◽  
...  
Keyword(s):  
Inflammatory Mediators ◽  
Neonatal Sepsis

scholarly journals Heat stress combined with lipopolysaccharide alter the activity and superficial molecules of peripheral monocytes

2019 ◽  
Vol 33 ◽  
pp. 205873841982889
Author(s):  
Jiajing Luo ◽  
Yi Chen ◽  
Chengjia Ding ◽  
Jialing Qiu ◽  
Yulan Chen ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Heat Stress ◽  
Western Blot ◽  
Inflammatory Mediators ◽  
Peripheral Blood ◽  
Heat Stroke ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Rt Pcr ◽  
Tnf Α

The purpose of this study was to focus on the underlying relationship between the hyperactivity for the peripheral monocytes and heat stroke by investigating the inflammatory oxidative activity of and the expression of superficial molecules. Peripheral blood samples were collected from 10 healthy adult volunteers. Human blood monocytes were isolated by density gradient centrifugation and sequent adherent culture. The objectives were divided into four groups: 43°C heat stress combined with lipopolysaccharide (LPS) group, 43°C heat stress group, LPS group, and control group. There were 10 cases in each group. An enzyme-linked immunosorbent assay (ELISA) test was used to measure the concentrations of supernatant inflammatory mediators (tumor necrosis factor alpha (TNF-α), interleukin-1β (IL-1β) and interleukin-10 (IL-10)). After loaded by 2,7-Dichlorodi-hydrofluorescein-diacetate (DCFHDA) fluorescent probe, intracellular reactive oxygen species (ROS) levels were determined by a flow cytometry. After fluorescent microspheres incubation, the phagocytosis of monocytes was observed under a fluorescent microscope. Respectively, the flow cytometry and Western blot were used to evaluate the level of triggering receptor expressed on myeloid cells-1 (TREM-1) and Toll-like receptor-4 (TLR-4) on the monocytes. Furthermore, the mRNA expression of TREM-1 and TLR-4 was detected by real-time polymerase chain reaction (RT-PCR). The heat stress combined with LPS stimulation promoted the peripheral monocytes to produce inflammatory mediators (TNF-α, IL-1β, and IL-10) and release ROS. Otherwise, such complex strike significantly suppressed the phagocytic activity of monocytes in peripheral blood. Moreover, the expression of TREM-1, TLR-4 and CD86 was measured by the flow cytometry on peripheral monocytes which were respectively promoted by the union of heat stress and LPS. The results of Western blot and RT-PCR demonstrated the similar kinetics on these superficial molecules (TREM-1, TLR-4, and CD86) stimulated by the combination of heat stress and LPS. The underlying mechanism of the dysfunction for the peripheral monocytes may be related to the abnormal expression of superficial molecules TREM-1, TLR-4, and CD86 on the monocytes induced by heat stress and LPS.

scholarly journals Differential Regulation of Eicosanoid and Endocannabinoid Production by Inflammatory Mediators in Human Choriodecidua

PLoS ONE ◽  
2016 ◽  
Vol 11 (2) ◽  
pp. e0148306 ◽  
Author(s):  
M. D. Mitchell ◽  
G. E. Rice ◽  
K. Vaswani ◽  
D. Kvaskoff ◽  
H. N. Peiris
Keyword(s):  
Inflammatory Mediators ◽  
Differential Regulation

Correlation between severity of migraine attacks and IgE level in peripheral blood

2016 ◽  
Vol 40 (3) ◽  
Author(s):  
Maryam Hassanpour Moghadam ◽  
Hamidreza Ardalani ◽  
Alireza Alehashemi ◽  
Mahboobeh Adami Dehkordi ◽  
Mojtaba Meshkat
Keyword(s):  
Inflammatory Mediators ◽  
Peripheral Blood ◽  
Migraine Headache ◽  
Clinical Symptoms ◽  
Allergic Sensitization ◽  
Allergic Diseases ◽  
University Hospital ◽  
Migraine Headaches ◽  
Treatment And Prevention ◽  
Significant Difference

Abstract: The correlation between allergic diseases and migraine is partially due to a better understanding of inflammatory mediators with vasoactive function that play an important role in these diseases. This study aimed to evaluate the correlation between allergic sensitization and severity of migraine.: This study was carried out on 212 patients who suffered from migraine headache in a university hospital in Mashhad, Iran. All of these patients were evaluated for allergic rhinitis (AR) by measuring the IgE level in peripheral blood and assessing the clinical symptoms of AR. Prevalence of AR in migraine patients and degree of allergic sensitization was assessed in this study.: The prevalence of AR in migraine patients was 78.30%. Total IgE levels in the peripheral blood in migraine patients with AR were found to be significantly (p<0.0001) higher than those without migraines. There was a significant difference between severity of AR (higher level of IgE) and severity of migraine attacks (p<0.0001), but there was not any significant difference between severity of AR and other factors.: We propose that inflammatory mediators play a key role in triggering migraine attacks. Thus, effective treatment of AR in migraine patients plays an important role in treatment and prevention of migraine headaches.

Differential Regulation of Inflammation by Inflammatory Mediators in Cystic Fibrosis Lung Epithelial Cells

2013 ◽  
Vol 33 (3) ◽  
pp. 121-129 ◽  
Author(s):  
Motohiro Tsuchiya ◽  
Parameet Kumar ◽  
Sharmistha Bhattacharyya ◽  
Sangbrita Chattoraj ◽  
Meera Srivastava ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Epithelial Cells ◽  
Inflammatory Mediators ◽  
Differential Regulation ◽  
Lung Epithelial Cells ◽  
Cystic Fibrosis Lung ◽  
Lung Epithelial
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