scholarly journals MICROBIAL FOOD SAFETY OF STREET FOODSAROUND THE VICINITY OF LSPU - SCCREGINA

2020 ◽  
Vol 8 (12) ◽  
pp. 75-80
Author(s):  
Regina E. Gloria ◽  
◽  
Ph. D Mary Jane D. Fuentes ◽  
DPA Zenaida O. Vitasa Ed. D. ◽  
◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Human Consumption ◽  
E Coli ◽  
Descriptive Research ◽  
A Value ◽  
Pathogenic Microbes ◽  
The Mean ◽  
High Level

Knowing the number of pathogenic microbes in the street foods such as Escherichia coli, coliform, and molds present in street foods around the vicinity of LSPU – SCC was the focused of the study. Determination of the quality of the street foods such as banana cue, kikiam, kwek – kwek, minane, and siomai of which has a high level of safety in the street food around the vicinity of LSPU – SCC were considered. This study utilized the Descriptive research design and the mean in testing and gathering of data. The results reveled that all the street foods tested were safe from E. coli with the mean of ocfu/g and all at lss than 10 cfu/g or 0 count which gathered Satisfactory remarks. However, the findings revealed that the banana cue, minane and siomai were safe from coliform while kwek and kikiam were not with a value of 2800 cfu/g. In addition, all the street foods tested were safe from molds and the total mean of all the microbes present were 987.47 cfu/g which means that the street foods were Unsatisfactory level with 100cfu/g and above count and revealed that only banana cue, minane, and siomai are safe for human consumption while large amount of microbes were found in kikiam, and kwek -kwek which means that they are not safe for human and may cause disease.

COLIFORM BACTERIA IN SEA WATER AND SHELLFISH: II. THE E. C. CONFIRMATION TEST FOR ESCHERICHIA COLI

1961 ◽  
Vol 7 (5) ◽  
pp. 733-739 ◽  
Author(s):  
A. D. Tennant ◽  
J. E. Reid ◽  
L. J. Rockwell ◽  
E. T. Bynoe
Keyword(s):  
Escherichia Coli ◽  
Sea Water ◽  
The United States ◽  
Coliform Bacteria ◽  
Simple Test ◽  
E Coli ◽  
Confirmation Test ◽  
The Mean ◽  
Test Cultures

Recently there has been considerable interest in Canada and the United States in the development of an effective, simple test for the estimation of Escherichia coli densities in shellfish and shellfish-growing waters as a better indication of "faecal" pollution than that provided by the coliform group. The fidelity of the E.C. confirmation test for the determination of E. coli densities was evaluated in 15 shellfish-growing areas which were classified as "polluted" or "unpolluted" according to generally accepted criteria. Coliform strains were isolated from 2765 E.C. gas positive confirmation test cultures and identified by IMViC tests. The mean fidelity of the test, as an indication of the presence of E. coli, was circa 90 per cent; contrary to expectations, however, the per cent recovery of E. coli was higher in unpolluted areas than in polluted areas, and varied considerably from region to region. Aerobacter aerogenes types I and II were the most common other E.C. gas positive coliform biotypes found. The usefulness of the confirmation test and the sanitary significance of these data are discussed.

scholarly journals Multiplex Fluorogenic Real-Time PCR for Detection and Quantification of Escherichia coli O157:H7 in Dairy Wastewater Wetlands

2002 ◽  
Vol 68 (10) ◽  
pp. 4853-4862 ◽  
Author(s):  
A. Mark Ibekwe ◽  
Pamela M. Watt ◽  
Catherine M. Grieve ◽  
Vijay K. Sharma ◽  
Steven R. Lyons
Keyword(s):  
Escherichia Coli ◽  
Detection Limit ◽  
The United States ◽  
Dairy Wastewater ◽  
Pcr Assay ◽  
E Coli ◽  
Quality Of Water ◽  
Plate Counts

ABSTRACT Surface water and groundwater are continuously used as sources of drinking water in many metropolitan areas of the United States. The quality of water from these sources may be reduced due to increases in contaminants such as Escherichia coli from urban and agricultural runoffs. In this study, a multiplex fluorogenic PCR assay was used to quantify E. coli O157:H7 in soil, manure, cow and calf feces, and dairy wastewater in an artificial wetland. Primers and probes were designed to amplify and quantify the Shiga-like toxin 1 (stx1) and 2 (stx2) genes and the intimin (eae) gene of E. coli O157:H7 in a single reaction. Primer specificity was confirmed with DNA from 33 E. coli O157:H7 and related strains with and without the three genes. A direct correlation was determined between the fluorescence threshold cycle (CT ) and the starting quantity of E. coli O157:H7 DNA. A similar correlation was observed between the CT and number of CFU per milliliter used in the PCR assay. A detection limit of 7.9 × 10−5 pg of E. coli O157:H7 DNA ml−1 equivalent to approximately 6.4 × 103 CFU of E. coli O157:H7 ml−1 based on plate counts was determined. Quantification of E. coli O157:H7 in soil, manure, feces, and wastewater was possible when cell numbers were ≥3.5 × 104 CFU g−1. E. coli O157:H7 levels detected in wetland samples decreased by about 2 logs between wetland influents and effluents. The detection limit of the assay in soil was improved to less than 10 CFU g−1 with a 16-h enrichment. These results indicate that the developed PCR assay is suitable for quantitative determination of E. coli O157:H7 in environmental samples and represents a considerable advancement in pathogen quantification in different ecosystems.

scholarly journals Microbiological quality of shellfish and evaluation of compact dry EC for detecting total coliforms and Escherichia coli

2020 ◽  
Vol 49 (1) ◽  
pp. 32-39 ◽  
Author(s):  
A. Vásquez García ◽  
S.H. Gomes de Sá ◽  
G. de Sousa Silva ◽  
J.E. Mejia Ballesteros ◽  
E. Barbieri ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Conventional Method ◽  
Microbiological Quality ◽  
Correlation Coefficients ◽  
Mean Values ◽  
Total Coliforms ◽  
E Coli ◽  
The Mean

The objective of this study was to evaluate the microbiological quality of oysters and mussels grown in Cananéia, Brazil, by analysing mesophiles, psychrothophic bacteria, moulds and yeasts, Staphylococcus aureus, and Salmonella spp., and to compare the efficiency of Compact Dry EC method and the conventional method for counting of total coliforms and Escherichia coli. The microbial analysis showed that the mean values of mesophilic counts were 3.14±0.81 log CFU g−1 for oysters and 3.92±0.90 for mussels; the mean values of psychrophilic counts were 2.78±0.75 log CFU g−1 for oysters and 3.22±0.75 log CFU g−1 for mussels; the mean values of mould and yeast counts were 3.70±0.58 log CFU g−1 for oysters and 3.33±0.81 log CFU g−1 for mussels. Salmonella spp. did not present positive results, and the maximal count of Staphylococcus aureus was 1.7 log CFU g−1, therefore, within the limits established in the legislation. The correlation coefficients between the Compact Dry EC method and conventional method were >0.87 for total coliform and E. coli counts for both types of shellfish. The data in this study show that the Compact Dry EC method is an acceptable alternative to conventional methods for enumeration of total coliforms and E. coli in shellfish.

scholarly journals Microbiological quality of seasoned chicken cuts using Escherichia coli and Salmonella spp. as quality indicators.

2020 ◽  
Vol 9 (11) ◽  
pp. e38091110013
Author(s):  
Flávio Barbosa da Silva ◽  
Bruna Ribeiro Arrais ◽  
Marcos Roberto Alves Ferreira ◽  
Iderval da Silva Júnior Sobrinho ◽  
Márcia Dias ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Microbiological Quality ◽  
Poultry Production ◽  
Salmonella Spp ◽  
Production Chain ◽  
E Coli ◽  
Important Health ◽  
Pathogenic Serotypes

Enteropathogens such as Salmonella spp. and Escherichia coli are important health challenges in the poultry production chain, because when installed in the production chain, they impair the safety of food supply. The determination of the microbiological quality of chicken meat, especially when marketed in spiced form, is necessary, given that consumer prefer this type of processed protein. This work aims to evaluate the microbiological quality of the meat of seasoned chicken marketed in the municipality of Rio Verde, Goias, using Salmonella spp. and Escherichia coli as target microorganisms, and considering as variables the type of establishment, validity date and presence of municipal inspection seal. From 80 analyzed samples, 30% (24/80) of samples were positive for Salmonella spp. and 55% (44/80) for E. coli. Regarding the type of establishment, it was observed that 27.45% (14/51) of supermarket samples and 34.48% (10/29) of meat store samples were contaminated by Salmonella spp. Considering E. coli, 49.01% (25/51) of supermarket samples and 65.55% (19/29) of meat store samples were positive for this pathogen. 80% (64/80) of the samples had the municipal inspection seal (MIS), and 83.75% (67/80) contained the expiration date on the label. The legislation in its narrative guarantee’s protection for the consumer with regard to the presence of pathogenic serotypes of these agents, however, it is worth noting that the effective action of health surveillance, and the constant laboratory investigation of the products are necessary.

scholarly journals Avaliação da qualidade da água de poços domésticos em comunidades rurais no Arquipélago de Marajó- PA

2020 ◽  
Vol 13 (5) ◽  
pp. 2462
Author(s):  
Marcelo Coelho Simões ◽  
Gundisalvo Piratoba Morales ◽  
Priscila Sanjuan De Medeiros Sarmento ◽  
Ilma Pastana Ferreira ◽  
Robson José Domingues ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rural Communities ◽  
High Sensitivity ◽  
Shallow Aquifer ◽  
Human Consumption ◽  
Technical Standards ◽  
E Coli ◽  
Marajó Island ◽  
Solids Content

O estudo trata da avaliação da qualidade da água de poços domésticos utilizados pelas comunidades rurais no arquipélago de Marajó- PA, a partir da análise dos parâmetros físico-químicos e microbiológicos. As amostras de água foram coletadas obedecendo as normas técnicas em vigor, ocorrendo no período de maior índice pluviométrico da região, com temperatura entre 23°C e 32 °C. Para cada amostra físico-química analisou-se: Potencial de hidrogênio (pH), Temperatura (T), Oxigênio Dissolvido (OD), Sólido Totais Dissolvidos (STD) e Condutividade Elétrica (CE). Para as análises microbiológicas as amostras foram coletadas utilizando recipientes previamente esterilizados, e a sua quantificação foi realizada usando de análise de membrana e Placa de Alta Sensibilidade 3M™ Petrifilm™ para identificar a presença de coliformes totais e Escherichia coli. Os dados foram interpretados usandoanálise estatística, realizando o teste de variância (ANOVA) e equivalentes não paramétricos (Mann Whitney e Kruskal wallis). A partir de cada análise, os valores de CE e STD apresentaram-se relativamente baixos, não evidenciando níveis de contaminação nesses poços, embora tenha sido identificada a presença de c. totais e E. coli, fato que limitaria o uso deste recurso para consumo humano. Os parâmetros em desacordo com valores de potabilidade podem estar relacionados com hábitos e manuseio inadequado na captação de água, além da falta de proteção desses poços com profundidade rasa de aquífero livre, pois, estão susceptíveis à entrada de poluentes derivadas de atividades antrópicas potencialmente poluidoras.  The evaluation of the quality of the water in the wells in the rural communities of the Archipelago of Marajó island - PA A B S T R A C TThe study deals with the evaluation of the quality of the water in the wells that are used by the rural communities in the archipelago of the Marajó island (PA), based on the analysis of the physico-chemical and microbiological. The water samples were collected according to the technical standards in force, during the period of the highest rainfall in the region, with a temperature of 23°C and 32 °C. For each sample, of analysis: the Potential of hydrogen (pH), Temperature (T), Dissolved Oxygen (OD), a Solid-Total solids content (a STD) and Electrical Conductivity (EC). For the microbiological analyses of the samples were collected using containers, pre-sterilized, and the quantification was carried out using the analysis of the membrane and the Plate of the High Sensitivity of the 3M™ Petrifilm™ to identify the presence of total coliforms and Escherichia coli. The data were interpreted by using a statistical analysis, using the test of variance (ANOVA), and the equivalent non-parametric (Mann Whitney e Kruskal wallis). From each analysis, the values of CE and STD showed to be relatively low, the levels of contamination in these wells, even though it has been identified that the presence of c. total and E. coli, a fact that woulding limit the use of water for human consumption. All the parameters are at odds with the values of drinking may be related to habits, and improper handling on the water, in addition to the lack of protection from these wells with the depth of the shallow aquifer is free, therefore, they are susceptible to the entry of any pollutant derived from anthropogenic activities.Keywords: Rural population; water resources; vulnerability. 

scholarly journals GAMBARAN ANGKA LEMPENG TOTAL DAN IDENTIFIKASI BAKTERI Escherichia coli PADA LOLOH BUNGA TELENG DI DESA PENGLIPURAN KECAMATAN BANGLI

2021 ◽  
Vol 9 (1) ◽  
pp. 12-18
Author(s):  
Nyoman Mastra
Keyword(s):  
Escherichia Coli ◽  
Key Words ◽  
Microbiological Quality ◽  
Plate Count ◽  
Sampling Techniques ◽  
E Coli ◽  
Descriptive Research ◽  
Total Plate Count ◽  
Laboratory Examinations

AbstractBackground : Teleng flower herb is a processed beverage made from teleng flower which is popular as a traditional drink from Penglipuran Village. As one of the beverage products, teleng flower herbs have to obtain consent from the BPOM before distributed and consumed. Purpose : this study was conducted to analyze the quality of microbiology of teleng flower herb. The analyzed parameters were total plate count (ALT) and E. coli bacteria identification. Method : this study used descriptive research with laboratory examinations which was conducted at the Bacteriology Laboratory. The 7 samples were obtained from producers with three times repetition so that it became 21 samples with sampling techniques were saturated sampling. Result : The ALT examination results show that 5 samples (71.4%) are not qualify and 2 samples (28.6%) are qualify based on BPOM Regulation Number 16 In 2016. The obtained results of E. coli identification show that there were 4 (57.1%) contain of E. coli pathogens and 3 samples (42.9%) are not contains of E. coli pathogens. It can be concluded that most of the hygiene of teleng flower herb at Penglipuran Village does not meet the requirements. Conclusion : Based on these results, we suggest to the producers of teleng flower herb to maintain the quality of teleng flower herb that they sell. Key words: teleng flower herb, microbiological quality, total plate count, E. coli

scholarly journals Determination of Escherichia coli Contamination with Chromocult Coliform Agar Showed a High Level of Discrimination Efficiency for Differing Fecal Pollution Levels in Tropical Waters of Kampala, Uganda

2000 ◽  
Vol 66 (2) ◽  
pp. 864-868 ◽  
Author(s):  
Dennis Byamukama ◽  
Frank Kansiime ◽  
Robert L. Mach ◽  
Andreas H. Farnleitner
Keyword(s):  
Escherichia Coli ◽  
Surface Waters ◽  
Fecal Pollution ◽  
Fecal Coliforms ◽  
E Coli ◽  
Pollution Levels ◽  
Lower Ability ◽  
Polluted Sites ◽  
High Level

ABSTRACT Escherichia coli, total coliforms, fecal coliforms, and sulfite-reducing anaerobic spore formers from different polluted sites in a tropical environment were determined in order to test for their indication ability for fecal contamination. Quantification of E. coli contamination with Chromocult coliform agar proved to be efficient and feasible for determining fecal pollutions in the investigated area within 24 h. The other microbial parameters showed a lower ability to differentiate sites and cannot be recommended for monitoring fecal pollution in the studied tropical surface waters.

scholarly journals Kandungan Bakteri Escherichia Coli Pada Air Rendaman Tahu Pedagang Kaki Lima Di Pasar Kasih Naikoten 1 Kota Kupang Tahun 2017

2018 ◽  
Vol 16 (1) ◽  
pp. 66-71
Author(s):  
Agustina Agustina ◽  
Albina Bare Telan ◽  
Frengki Mboro
Keyword(s):  
Escherichia Coli ◽  
Personal Hygiene ◽  
Raw Water ◽  
Street Vendors ◽  
Physical Quality ◽  
E Coli ◽  
Descriptive Research ◽  
Survey Approach ◽  
Washing Water

One of the most common contaminants in food is Escherichia coli. These bacteria come from human and animal feces, are infected with food because of the unhygienic behavior of the handler, the washing of unclean equipment, the health of food processors and handlers and the use of washing water containing Escherichia coli. The purpose of this study was to assess the physical quality of tofu soaking raw water, assess the sanitation of tofu soaking containers and calculate the amount of E. coli soaked tofu bacteria in street vendors in the Love Market Naikoten 1 Kota Kupang in 2017. This type of research was descriptive research. with a survey approach with the size of the sample, namely 23 tofu traders in the Pasar Naik Naiken Kota Kupang. The results showed that the physical quality of tofu soaking raw water was 70% fulfilling the requirements, and 30% did not meet the requirements, soaking sanitation containers knew 66% good enough category, 17% good category and 17% poor category, E. coli bacteria content checked 4% eligible categories and 96% categories did not meet the requirements. Conclude that the physical quality of raw tofu soaking water meets the requirements, sanitation of containers used to soak the tofu well enough and the content of E. coli bacteria does not meet the requirements. The advice given is to pay attention to personal hygiene in touching food, hand hygiene and always closing the container at any time.

scholarly journals The Problems and Needs of Patients Diagnosed with Cancer and Their Caregivers

2020 ◽  
Vol 18 (1) ◽  
pp. 87
Author(s):  
Anna Lewandowska ◽  
Grzegorz Rudzki ◽  
Tomasz Lewandowski ◽  
Sławomir Rudzki
Keyword(s):  
Cancer Patients ◽  
Medical Information ◽  
Unmet Needs ◽  
Public Healthcare ◽  
Literature Analysis ◽  
The Public ◽  
The Mean ◽  
High Level ◽  
Patients Experience

(1) Background: As the literature analysis shows, cancer patients experience a variety of different needs. Each patient reacts differently to the hardships of the illness. Assessment of needs allows providing more effective support, relevant to every person’s individual experience, and is necessary for setting priorities for resource allocation, for planning and conducting holistic care, i.e., care designed to improve a patient’s quality of life in a significant way. (2) Patients and Methods: A population survey was conducted between 2018 and 2020. Cancer patients, as well as their caregivers, received an invitation to take part in the research, so their problems and needs could be assessed. (3) Results: The study involved 800 patients, 78% women and 22% men. 66% of the subjects were village residents, while 34%—city residents. The mean age of patients was 62 years, SD = 11.8. The patients received proper treatment within the public healthcare. The surveyed group of caregivers was 88% women and 12% men, 36% village residents and 64% city residents. Subjects were averagely 57 years old, SD 7.8. At the time of diagnosis, the subjects most often felt anxiety, despair, depression, feelings of helplessness (46%, 95% CI: 40–48). During illness and treatment, the subjects most often felt fatigued (79%, 95% CI: 70–80). Analysis of needs showed that 93% (95% CI: 89–97) of patients experienced a certain level of need for help in one or more aspects. (4) Conclusions: Patients diagnosed with cancer have a high level of unmet needs, especially in terms of psychological support and medical information. Their caregivers also experience needs and concerns regarding the disease. Caregivers should be made aware of the health consequences of cancer and consider appropriate supportive care for their loved ones.

scholarly journals The fate of Si and Fe while nuclear glass alters with steel and clay

2021 ◽  
Vol 5 (1) ◽  
Author(s):  
C. Carriere ◽  
P. Dillmann ◽  
S. Gin ◽  
D. Neff ◽  
L. Gentaz ◽  
...  
Keyword(s):  
Dissolution Rate ◽  
Protective Layer ◽  
Glass Dissolution ◽  
A Value ◽  
Waste Forms ◽  
Controlling Mechanism ◽  
Geological Repository ◽  
The Mean ◽  
High Level ◽  
Initial Dissolution Rate

AbstractThe French concept developed to dispose high-level radioactive waste in geological repository relies on glassy waste forms, isolated from the claystone host rock by steel containers. Understanding interactions between glass and surrounding materials is key for assessing the performance of a such system. Here, isotopically tagged SON68 glass, steel and claystone were studied through an integrated mockup conducted at 50 °C for 2.5 years. Post-mortem analyses were performed from nanometric to millimetric scales using TEM, STXM, ToF-SIMS and SEM techniques. The glass alteration layer consisted of a crystallized Fe-rich smectite mineral, close to nontronite, supporting a dissolution/reprecipitation controlling mechanism for glass alteration. The mean glass dissolution rate ranged between 1.6 × 10−2 g m−2 d−1 to 3.0 × 10−2 g m−2 d−1, a value only 3–5 times lower than the initial dissolution rate. Thermodynamic calculations highlighted a competition between nontronite and protective gel, explaining why in the present conditions the formation of a protective layer is prevented.

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