scholarly journals TO EVALUATE THE ANTIMICROBIAL EFFICACY OF SIX MOUTHWASHES AGAINST STAPHYLOCOCCUS AUREUS AND STREPTOCOCCUS MUTANS – IN INVITRO STUDY

2017 ◽  
Vol 5 (3) ◽  
pp. 2275-2280
Author(s):  
Dr.A. Sudheer ◽  
◽  
Dr.N.V.V.Satya Bhushan ◽  
Dr.U.Siva Kalyan ◽  
Dr.KhoChai Chiang ◽  
...  
Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
HH Alfa ◽  
R Arroo ◽  
S Walsh ◽  
K Ruparelia ◽  
A Bhambra ◽  
...  

2020 ◽  
Vol 3 (1) ◽  
pp. 50-57
Author(s):  
Vriezka Mierza ◽  
Sudewi Sudewi

Kapulaga lokal (Amomum compactum Sol. ex Manton) mampu menghambat pertumbuhan cendawan dan senyawa sineol. kapulaga lokal yang bersifat sebagai anticendawan dan mengandung antibakteri.Penelitian ini bertujuan untuk mengetahui ekstrak etanol buah kapulaga yang diformulasikan menjadi sediaan obat kumur yang dapat menghambat pertumbuhan bakteri Staphylococcus aureus dan Streptococcus mutanspada konsentrasi tertentu. Adapun metode dari penelitian ini adalah dengan menggunakan pengujian aktivitas antibakteri Staphylococcus aureus dan Streptococcus mutans dari sediaan obat kumur ekstrak etanol buah kapulaga.Sediaan dievaluasi stabilitas fisik penyimpanan selama 4 minggu, uji pH dan pengujian antibakteri. Seluruh sediaan obat kumur ekstrak etanol buah kapulaga yang dibuat memiliki bentuk fisik yang baik dan stabil pada penyimpanan selama 4 minggu, tidak memiliki pH yang sesuai dan hasil uji aktivitas antibakteri sedian obat kumur ekstrak etanol buah kapulaga dengan konsetrasi ekstrak 2%, 4%, dan 6% pada Streptococcus mutans, menghasikan diameter daya hambatnya berurut-turut 8,42 mm; 9,03 mm; dan 9,48 mm, sedangkan Staphylococcus aureus diameter daya hambatnya secara  berurutan  12 mm, 11 mm, dan 10 mm. Hasil penelitian ini menyimpulkan bahwa ekstrak etanol buah kapulaga dapat diformulasikan menjadi obat kumur  dengan konsentrasi 2%, 4%, dan 6% yang memiliki daya hambat kategori sedang terhadap bakteri  Streptococcus mutans dan  memiliki daya hambat kategori kuat terhadap bakteri Staphylococcus aureus.


Author(s):  
Damajanty H. C. Pangemanan ◽  
Christy Mintjelungan

Background: Dental caries and periodontal disease can be caused by bacteria that attack hard and soft tissues in the oral cavity like Staphylococcus aureus and Streptococcus mutans bacteria One way to prevent infections caused by the bacteria are by using mouthwash. Currently, mouthwash that is widely used is mouthwash containing chlorhexidine, which has side effects in the form of tooth staining when used prolonged. The need for alternative medicines from nature that effectively cope with polymicrobial infections. One of the marine products that has pharmaceutical properties is squid, especially the ink it produces.Methods: This research is a pure experimental study (true experimental design) with a randomized pretest-posttest control group design. Squid ink Loligo sp. extract, obtained by extracting squid ink Loligo sp., with maceration method using 96% ethanol solvent. This research uses serial dilution method with spectrophotometric testing method.Results: After measuring the turbidity value in each treatment tube, it was found that at a concentration of 1.56% squid ink Loligo sp. extract began to inhibit the growth of Staphylococcus aureus and Streptococcus mutans bacteria.  This is proven because the absorbance value after and before incubation is fixed.Conclusions: The minimum inhibitory concentration of squid ink Loligo sp. extract on the growth of Staphylococcus aureus and Streptococcus mutants at a concentration of 1.56%.


2006 ◽  
Vol 28 (62) ◽  
Author(s):  
Ana Maria Trindade Grégio ◽  
Edilene Sayuri Miyamoto Fortes ◽  
Edvaldo A. Ribeiro Rosa ◽  
Rossana Baggio Simeoni ◽  
Rosemeire Takaki Rosa

A fitoterapia tem sido alvo de investigações científicas, especialmente em países do oriente. No Brasil, também ocorre um especial interesse da comunidade científica quanto ao uso, efeito e propriedades farmacológicas das plantas medicinais, tendo o seu uso direcionado ao tratamento de várias doenças. A microbiota bucal é um dos locais em que há uma maior variedade de microrganismos que podem, por um desequilíbrio, tornar-se patogênicos. Baseado nisso, um estudo do extrato da Zingiber officinalle foi realizado para avaliar o seu potencial antimicrobiano, com microrganismos comumente encontrados na cavidade bucal, no qual foi obtida a concentração mínima inibitória do extrato de gengibre frente a alguns tipos de patógenos presentes na microbiota bucal do ser humano. Foi utilizado o método microbiológico no qual os extratos glicólico e hidroalcoólico do gengibre sofreram diluições seriadas no meio de cultura (BHI) para uma posterior inoculação dos microrganismos, Streptococcus mutans, Staphylococcus aureus, Escherichia coli e Candida albicans. Obteve-se a concentração mínima inibitória dos extratos de gengibre, tanto na sua forma glicólica quanto hidroalcoólica. A dose mínima necessária para inibir o crescimento dos vários microrganismos testados foi de 5mg/ml dos dois extratos estudados. O extrato da Zingiber officinalle apresentou atividade antibacteriana e antifúngica relevante para a Odontologia, podendo contribuir para o tratamento de doenças causadas por esses microrganismos presentes na cavidade bucal.


2018 ◽  
Vol 40 ◽  
pp. 455-460 ◽  
Author(s):  
Kely Karina Belato ◽  
Jonatas Rafael de Oliveira ◽  
Fernanda Saraiva de Oliveira ◽  
Luciane Dias de Oliveira ◽  
Samira Esteves Afonso Camargo

2016 ◽  
Vol 33 (3) ◽  
pp. 140-145
Author(s):  
Mst Laila Akter Banu ◽  
AKM Bashar ◽  
Md Mujibur Rahman Howlader ◽  
Md Shamsul Alam ◽  
Md Ashraf Hussain

Microorganisms, usually from the dental caries, are the main sources of diseases in dental pulp (root canals) and periapical region. Facultative bacteria and fungi have been identified in therapy resistant persistent endodontic infection. The objectives of this study was to evaluate the antimicrobial efficacy of Mineral Tri Oxide Aggregate (MTA) against therapy resistant endodontic microorganisms. The efficacy of MTA was also compared with that of calcium hydroxide. Six standard bacterial stains were used: Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, bacillus subtilis, Candida albicans and Enterococcus faecalis. The agar diffusion method on Muller- Hilton media was employed. The plates containing media were inoculated with the specified bacterial suspensions. Two standard holes were prepared on each microorganism inoculated plate with a copper puncher and one hole was completely filled with MTA & the other with Ca (OH)2 . The plates were then kept at environmental temperature for one hour to ensure prediffusion and then incubated at 370C for 24 hours. After 24 hours, the diameters of inhibition zones were measured. Tests were replicated for thirty times for each sample and mean values were taken. Zone of inhibition as measured for MTA and Ca (OH)2 were statistically analyzed with Student’s t-Test and Post Hoc Games Howell Test and were presented as mean ± SD to compare of efficacy of MTA and calcium hydroxide on different microorganisms. Both MTA and Ca(OH)2 were found to produce zone of inhibition against Staphylococcus aureus (ATCC 25923), Pseudomonas aeruginosa (ATCC 27853), , Bacillus subtilis (BTCC 17 ), and Candida albicans (BTCC 493). MTA showed highest activity against S. aureus and lowest activity against P. aeruginosa which was similar to the activity range of Ca (OH)2 against the mentioned organisms. But both of them failed to produce any activity against E. coli and. E. faecalis. MTA was found to produce a lower efficacy than Ca (OH)2 while comparing the zone of inhibition between them and statistically it was significant. Mineral Tri Oxide Aggregate (MTA) showed antimicrobial efficacy against some therapy resistant microorganisms but it did not show antimicrobial efficacy against Escherichia coli and Enterococcus faecalis. MTA was found to produce a lower antimicrobial efficacy than Ca (OH)2.J Bangladesh Coll Phys Surg 2015; 33(3): 140-145


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