scholarly journals UJI AKTIVITAS ANTIBAKTERI SEDIAAN OBAT KUMUR EKSTRAK ETANOL BUAH KAPULAGA (Amomum compactum Sol. ex Maton) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus DAN Streptococcus mutans

2020 ◽  
Vol 3 (1) ◽  
pp. 50-57
Author(s):  
Vriezka Mierza ◽  
Sudewi Sudewi
Keyword(s):  
Staphylococcus Aureus ◽  
Streptococcus Mutans ◽  
Amomum Compactum

Kapulaga lokal (Amomum compactum Sol. ex Manton) mampu menghambat pertumbuhan cendawan dan senyawa sineol. kapulaga lokal yang bersifat sebagai anticendawan dan mengandung antibakteri.Penelitian ini bertujuan untuk mengetahui ekstrak etanol buah kapulaga yang diformulasikan menjadi sediaan obat kumur yang dapat menghambat pertumbuhan bakteri Staphylococcus aureus dan Streptococcus mutanspada konsentrasi tertentu. Adapun metode dari penelitian ini adalah dengan menggunakan pengujian aktivitas antibakteri Staphylococcus aureus dan Streptococcus mutans dari sediaan obat kumur ekstrak etanol buah kapulaga.Sediaan dievaluasi stabilitas fisik penyimpanan selama 4 minggu, uji pH dan pengujian antibakteri. Seluruh sediaan obat kumur ekstrak etanol buah kapulaga yang dibuat memiliki bentuk fisik yang baik dan stabil pada penyimpanan selama 4 minggu, tidak memiliki pH yang sesuai dan hasil uji aktivitas antibakteri sedian obat kumur ekstrak etanol buah kapulaga dengan konsetrasi ekstrak 2%, 4%, dan 6% pada Streptococcus mutans, menghasikan diameter daya hambatnya berurut-turut 8,42 mm; 9,03 mm; dan 9,48 mm, sedangkan Staphylococcus aureus diameter daya hambatnya secara  berurutan  12 mm, 11 mm, dan 10 mm. Hasil penelitian ini menyimpulkan bahwa ekstrak etanol buah kapulaga dapat diformulasikan menjadi obat kumur  dengan konsentrasi 2%, 4%, dan 6% yang memiliki daya hambat kategori sedang terhadap bakteri  Streptococcus mutans dan  memiliki daya hambat kategori kuat terhadap bakteri Staphylococcus aureus.

scholarly journals Minimum inhibitory concentration of squid ink Loligo sp. extract on growth of Staphylococcus aureus and Streptococcus mutans bacteria

2020 ◽  
Vol 8 (3) ◽  
pp. 801
Author(s):  
Damajanty H. C. Pangemanan ◽  
Christy Mintjelungan
Keyword(s):  
Staphylococcus Aureus ◽  
Minimum Inhibitory Concentration ◽  
Streptococcus Mutans ◽  
Soft Tissues ◽  
Inhibitory Concentration ◽  
Control Group ◽  
Dilution Method ◽  
Control Group Design ◽  
Alternative Medicines ◽  
Pharmaceutical Properties

Background: Dental caries and periodontal disease can be caused by bacteria that attack hard and soft tissues in the oral cavity like Staphylococcus aureus and Streptococcus mutans bacteria One way to prevent infections caused by the bacteria are by using mouthwash. Currently, mouthwash that is widely used is mouthwash containing chlorhexidine, which has side effects in the form of tooth staining when used prolonged. The need for alternative medicines from nature that effectively cope with polymicrobial infections. One of the marine products that has pharmaceutical properties is squid, especially the ink it produces.Methods: This research is a pure experimental study (true experimental design) with a randomized pretest-posttest control group design. Squid ink Loligo sp. extract, obtained by extracting squid ink Loligo sp., with maceration method using 96% ethanol solvent. This research uses serial dilution method with spectrophotometric testing method.Results: After measuring the turbidity value in each treatment tube, it was found that at a concentration of 1.56% squid ink Loligo sp. extract began to inhibit the growth of Staphylococcus aureus and Streptococcus mutans bacteria.  This is proven because the absorbance value after and before incubation is fixed.Conclusions: The minimum inhibitory concentration of squid ink Loligo sp. extract on the growth of Staphylococcus aureus and Streptococcus mutants at a concentration of 1.56%.

scholarly journals AÇÃO ANTIMICROBIANA DO Zingiber officinalle FRENTE À MICROBIOTA BUCAL

2006 ◽  
Vol 28 (62) ◽  
Author(s):  
Ana Maria Trindade Grégio ◽  
Edilene Sayuri Miyamoto Fortes ◽  
Edvaldo A. Ribeiro Rosa ◽  
Rossana Baggio Simeoni ◽  
Rosemeire Takaki Rosa
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Streptococcus Mutans

A fitoterapia tem sido alvo de investigações científicas, especialmente em países do oriente. No Brasil, também ocorre um especial interesse da comunidade científica quanto ao uso, efeito e propriedades farmacológicas das plantas medicinais, tendo o seu uso direcionado ao tratamento de várias doenças. A microbiota bucal é um dos locais em que há uma maior variedade de microrganismos que podem, por um desequilíbrio, tornar-se patogênicos. Baseado nisso, um estudo do extrato da Zingiber officinalle foi realizado para avaliar o seu potencial antimicrobiano, com microrganismos comumente encontrados na cavidade bucal, no qual foi obtida a concentração mínima inibitória do extrato de gengibre frente a alguns tipos de patógenos presentes na microbiota bucal do ser humano. Foi utilizado o método microbiológico no qual os extratos glicólico e hidroalcoólico do gengibre sofreram diluições seriadas no meio de cultura (BHI) para uma posterior inoculação dos microrganismos, Streptococcus mutans, Staphylococcus aureus, Escherichia coli e Candida albicans. Obteve-se a concentração mínima inibitória dos extratos de gengibre, tanto na sua forma glicólica quanto hidroalcoólica. A dose mínima necessária para inibir o crescimento dos vários microrganismos testados foi de 5mg/ml dos dois extratos estudados. O extrato da Zingiber officinalle apresentou atividade antibacteriana e antifúngica relevante para a Odontologia, podendo contribuir para o tratamento de doenças causadas por esses microrganismos presentes na cavidade bucal.

scholarly journals Cytotoxicity and genotoxicity of thymol verified in murine macrophages (RAW 264.7) after antimicrobial analysis in Candida albicans, Staphylococcus aureus, and Streptococcus mutans

2018 ◽  
Vol 40 ◽  
pp. 455-460 ◽  
Author(s):  
Kely Karina Belato ◽  
Jonatas Rafael de Oliveira ◽  
Fernanda Saraiva de Oliveira ◽  
Luciane Dias de Oliveira ◽  
Samira Esteves Afonso Camargo
Keyword(s):  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Streptococcus Mutans ◽  
Raw 264.7 ◽  
Murine Macrophages

scholarly journals The Antibacterial Effectiveness of Red Ginger (Zingiber Officinale Roscoe) Essential Oil in Inhibiting The Growth of Staphylococcus Aureus and Streptococcus Mutans

2021 ◽  
Vol 7 (2) ◽  
pp. 364-373
Author(s):  
Shavilla Lukita ◽  
Winda Khosasi ◽  
Chandra Susanto ◽  
Florenly
Keyword(s):  
Staphylococcus Aureus ◽  
Essential Oil ◽  
Streptococcus Mutans ◽  
Zingiber Officinale ◽  
Diffusion Method ◽  
Ginger Extract ◽  
Zingiber Officinale Roscoe ◽  
Red Ginger ◽  
Post Hoc

Red ginger extract has a category strong antibacterial effect on Staphylococcusaureus and Streptococcus mutans. Red ginger essential oil has the potential forstronger inhibition. This study aims to compare the antibacterial effectiveness of redginger essential oil against Staphylococcus aureus and Streptococcus mutans. Thedesign of this study was a laboratory experimental design with a factorial completelyrandomized design. The red ginger used in this study was proven to be a species ofZingiber officinale Roscoe. The production of essential oils in this study uses thesteam distillation method. The content of secondary metabolites in red ginger wastested quantitatively by the GC-MS method. Determination of antibacterial activityusing the disc diffusion method. The data were processed using the SPSS 21.0program. The normality of data distribution was tested with the Shapiro-Wilk test,followed by one-way ANOVA, Levene's test, and the Tukey HSD Post Hoc Test. Theresults of the antibacterial test of red ginger essential oil against Staphylococcusaureus (21.21mm ± 0.315) and Streptoococcus mutans (23.43mm ± 0.189) provedthat the inhibition power of the category was very strong at a concentration of 75%.

scholarly journals Antimicrobial activity of oregan and clove essential oils against some foodborne pathogens

2020 ◽  
Vol 41 (1) ◽  
pp. 3
Author(s):  
Flavia Debiagi ◽  
Renata K.T. Kobayashi ◽  
Gerson Nakazato ◽  
Luciano Aparecido Panagio ◽  
Suzana Mali
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Aspergillus Niger ◽  
Essential Oils ◽  
Bacillus Cereus ◽  
Streptococcus Mutans ◽  
Enterococcus Faecalis ◽  
Foodborne Pathogens ◽  
Penicillium Citrinum

A preferência mundial para alimentos mais saudáveis e livres de aditivos químicos pelos consumidores, associada ao aumento da resistência bacteriana, resultaram na necessidade de medidas alternativas no setor de alimentos. Os óleos correspondem a antimicrobianos naturais e constituem uma classe emergente como substitutos dos produtos químicos sintéticos na conservação de alimentos. O objetivo deste trabalho foi avaliar a atividade antimicrobiana de óleos essenciais de orégano (OEO) e cravo (CEO ) frente a bactérias, fungos e leveduras de importância no setor de alimentos. OEO e CEO foram avaliados in natura (IN) e após processamento térmico (TP) a 120 o C por 5 minutos. Para avaliar a atividade antimicrobiana frente a cada microrganismo empregou-se o método de discodifusão e o teste de microdiluição em caldo (MIC). Tanto o OEO quanto o CEO apresentaram zonas de inibição semelhantes para amostras IN e TP, indicando que a atividade antimicrobiana desses óleos são resistentes a altas temperaturas. Os microrganismos mais sensíveis para ambos os óleos essenciais foram os fungos (Aspergillus niger, Penicillium citrinum e Candida albicans), seguidos por Staphylococcus aureus, Bacillus cereus e Staphylococcus aureus resistente à meticilina (MRSA). Já as cepas Streptococcus mutans e Enterococcus faecalis apresentaram uma maior resistência frente à atividade antimicrobiana dos óleos essenciais. Em geral, os maiores halos de inibição e menores valores de MIC foram obtidos quando empregado o OEO, sugerindo uma maior atividade microbiana do mesmo quando comparado ao CEO

scholarly journals Adherence of Staphylococcus aureus Is Enhanced by an Endogenous Secreted Protein with Broad Binding Activity

1999 ◽  
Vol 181 (9) ◽  
pp. 2840-2845 ◽  
Author(s):  
Marco Palma ◽  
Axana Haggar ◽  
Jan-Ingmar Flock
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Epithelial Cells ◽  
Streptococcus Mutans ◽  
Staphylococcus Epidermidis ◽  
Plasma Proteins ◽  
Binding Activity ◽  
Secreted Protein ◽  
Α Chain ◽  
Oligomeric Forms

ABSTRACT A novel mechanism for enhancement of adherence ofStaphylococcus aureus to host components is described. A secreted protein, Eap (extracellular adherence protein), was purified from the supernatant of S. aureus Newman and found to be able to bind to at least seven plasma proteins, e.g., fibronectin, the α-chain of fibrinogen, and prothrombin, and to the surface ofS. aureus. Eap bound much less to cells ofStaphylococcus epidermidis, Streptococcus mutans, or Escherichia coli. The protein can form oligomeric forms and is able to cause agglutination of S. aureus. Binding of S. aureus to fibroblasts and epithelial cells was significantly enhanced by addition of Eap, presumably due to its affinity both for plasma proteins on the cells and for the bacteria.

scholarly journals Antimicrobial activity of mouth rinses incorporating zein coated magnesium oxide nanoparticles

2020 ◽  
Author(s):  
Ghada H Naguib ◽  
Mohamed T Hamed ◽  
Abdulkarim A Hatrom ◽  
Abeer M Alnowaiser ◽  
Sahar MN Bukhary ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Streptococcus Mutans ◽  
Enterococcus Faecalis ◽  
Antimicrobial Effect ◽  
Oral Bacteria ◽  
Disc Diffusion ◽  
Diffusion Test ◽  
Mgo Nanoparticles

Abstract Background The addition of antibacterial agents to oral prophylaxis materials showed inhibition of plaque accumulation and bacterial acid production. This study aimed to test the antimicrobial effect of distinctive oral mouth washes on an exemplary of oral bacteria after the addition of zein-coated MgO nanoparticles.Materials and Methods Three hundred and twelve groups were used in this study. MgO nanoparticles in five concentrations with zein and without zein-coating, were incorporated in three oral mouth washes: Listerine Zero, Listerine Total Control and Oral B in the mass percentages of 0.3%, 0.5%, 1%, 2%, 5% and 10%, in addition to controls with no MgO nanoparticles. The three mouthwashes with variable concentrations were studied in opposition of Staphylococcus aureus, Streptococcus mutans, Enterococcus faecalis and Candida albicans using two different tests, the Disc Diffusion Test (DDT) and Direct Contact Test (DCT). Data was analyzed with Kruskall-Wallis and Mann-Whitney U tests.Results Results showed a highly significant statistical difference of antimicrobial activity for all tested mouth washes with Zein coated MgO nanoparticles on Streptococcus mutans, Staphylococcus aureus, Enterococcus faecalis, and Candida albicans in the disc diffusion test. While in the DCT, all tested mouth washes with MgO nanoparticles with and without zein coating showed antimicrobial activity on all tested microorganisms.Conclusion Zein coated MgO nanoparticles is a potent antimicrobial agent when added to oral mouth washes. It is recommended to incorporate Zein MgO nanoparticles to Oral mouth washes to improve its antibacterial property.

scholarly journals Atividade antimicrobiana do óleo de copaifera officinalis sobre bactérias do biofilme oral

2016 ◽  
Vol 15 (1) ◽  
pp. 34
Author(s):  
Camila Oliveira ◽  
Letícia Campos ◽  
Hemilliany Duarte ◽  
Louise Bezerra ◽  
Lorenna Brandt ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Streptococcus Mutans ◽  
Tween 80

<p class="WW-Padro">As plantas medicinais vêm sendo utilizadas como alternativa no tratamento de várias doenças, o óleo da <em>Copaífera Officinalis</em> é indicado para diversos fins farmacológicos, sendo sua ação antimicrobiana o alvo do presente estudo. <strong>Objetivo:</strong> Analisar a atividade inibitória e bactericida do óleo frente aos<em> Streptococcus mutans </em>(ATCC 25175)<em> </em>e <em>Staphylococcus aureus </em>(ATCC 25923). <strong>Material e Métodos:</strong> A atividade antimicrobiana do óleo sobre os microorganismos foi realizada a partir da técnica de microdiluição em caldo, por meio da execução das seguintes etapas: preparação do inóculo, das substâncias (óleo da <em>Copaífera Officinalis</em>, digluconato de clorexidina e tween 80), determinação da CIM (Concentração Inibitória Mínima) e da CBM (Concentração Bactericida Mínima). Para determinação da CIM (considerada a menor concentração do óleo capaz de inibir o crescimento bacteriano), foram utilizadas placas de 96 poços e inseriu-se 100μL de caldo, 100μL da diluição dos óleos essenciais no primeiro poço e 10μL da suspensão bacteriana (1,5x106 microrganismos/ mL). A CBM foi considerada a menor concentração do óleo capaz de matar as bactérias. <strong>Resultados: </strong>A CIM do óleo da <em>Copaífera Officinalis</em> diante do <em>S. mutans </em>foi 30.000 µg/Ml, não apresentando sobre esse microorganismo ação bactericida, verificada através da CBM. A CIM e a CBM do <em>S. Aureus</em> foram 15.000 µg/Ml. <strong>Conclusão:</strong> O óleo da <em>Copaífera Officinalis</em> demostrou atividade bacteriostática sobre o <em>S. mutans e</em> <em>S. aureus</em>.</p>

scholarly journals TO EVALUATE THE ANTIMICROBIAL EFFICACY OF SIX MOUTHWASHES AGAINST STAPHYLOCOCCUS AUREUS AND STREPTOCOCCUS MUTANS – IN INVITRO STUDY

2017 ◽  
Vol 5 (3) ◽  
pp. 2275-2280
Author(s):  
Dr.A. Sudheer ◽  
◽  
Dr.N.V.V.Satya Bhushan ◽  
Dr.U.Siva Kalyan ◽  
Dr.KhoChai Chiang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Streptococcus Mutans ◽  
Antimicrobial Efficacy
Sign in / Sign up

Export Citation Format

Share Document