Assessment of Biotechnological Potential of Cyanobacteria and Microalgae Strains from IPPAS Culture Collection

2019 ◽  
pp. 12-29
Author(s):  
R.A. Sidorov ◽  
A.Yu. Starikov ◽  
A.S. Voronkov ◽  
A.S. Medvedeva ◽  
Z.V. Krivova ◽  
...  
Keyword(s):  
Biochemical Composition ◽  
Wide Spectrum ◽  
Food Additives ◽  
Mature Spore ◽  
High Growth ◽  
Biomass Productivity ◽  
Culture Collection ◽  
Growth Characteristics ◽  
Russian Science ◽  
Biotechnological Potential

As a result of screening of spore-forming bacteria, a B-13186 strain with a wide spectrum of antagonistic activity identified as Brevibacillus laterosporus by the analysis of sequences of variable sites of 16S rRNA was selected. Morphological, cultural and biochemical characteristics of the strain were studied. A distinctive feature of the strain is the presence of a canoe-like inclusion formed in sporangia and attached to the mature spore, as well as the ability to synthesize round-shaped crystalline inclusions. The strain was shown to be active against various species of gram-positive bacteria including A search for strains capable of simultaneously producing high amounts of several biologically valuable compounds and/or having high biomass productivity has been carried out. The growth characteristics and biochemical composition of 12 microalgae and cyanobacteria strains from the IPPAS Collection in the exponential and stationary growth phases were studied. All the strains had high growth rates (doubling time 6-22 h). The strains of Cyanobacterium sp. IPPAS B-1200, Chlorella sp. IPPAS C-1210, Nannochloris sp. IPPAS C-1509, Cyanidium caldarium IPPAS P-510 and Vischeria sp. IPPAS H-242 demonstrated the highest biotechnological potential and can be used for the production of various types of biofuel, pigments, feed and food additives, including those with high content of eicosapentaenoic (20:5 Δ5,8,11,14,17) acid content. microalgae, cyanobacteria, biochemical composition, fatty acids, valuable metabolites, growth characteristics This work was supported by a grant from the Russian Science Foundation [no. 14-14-00904]

scholarly journals COLMENA: A Culture Collection of Native Microorganisms for Harnessing the Agro-Biotechnological Potential in Soils and Contributing to Food Security

Diversity ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 337
Author(s):  
Sergio de los Santos-Villalobos ◽  
Alondra María Díaz-Rodríguez ◽  
María Fernanda Ávila-Mascareño ◽  
Andrea Denisse Martínez-Vidales ◽  
Fannie Isela Parra-Cota
Keyword(s):  
Food Security ◽  
Plant Growth ◽  
Field Trials ◽  
Culture Collection ◽  
Microbial Culture ◽  
Biotechnological Potential ◽  
Global Food Security ◽  
Beneficial Effects ◽  
Plant Growth Promoting

COLMENA is a microbial culture collection dedicated to the characterization, classification, preservation, and transferal of native microorganisms isolated from various agro-systems and other ecosystems in Mexico. This collection aims to protect microbial diversity, reducing soil degradation, but also exploiting its agro-biotechnological potential. So far, COLMENA has isolated and cryopreserved soil microorganisms from different crops in two major agricultural regions in Mexico, the Yaqui Valley, Sonora, and the Fuerte Valley, Sinaloa. COLMENA has specialized in the identification and characterization of microbial strains with metabolic capacities related to the promotion of plant growth and the biocontrol of phytopathogens. Thus, COLMENA has identified several promising plant growth-promoting microbial (PGPM) strains due to their metabolic and genetic potentials and their beneficial effects in vivo and field trials. These findings demonstrate the biotechnological potential of these strains for their future use in profitable agricultural alternatives focused on enhancing global food security. To share the knowledge and results of the COLMENA team’s scientific research, a virtual platform was created, where the database of the studied and preserved microorganisms is available to professionals, researchers, agricultural workers, and anyone who is interested.

scholarly journals Fire effects on soil system functioning: new insights and future challenges

2005 ◽  
Vol 14 (4) ◽  
pp. 339 ◽  
Author(s):  
Stefan H. Doerr ◽  
Artemi Cerdá
Keyword(s):  
Wide Spectrum ◽  
Biological Effects ◽  
Aggregate Stability ◽  
Biomass Productivity ◽  
Strong Relationship ◽  
Fire Effects ◽  
Future Research ◽  
Microbial Composition ◽  
Soil System ◽  
Diverse Field

Fire affects entire ecosystems – their flora, fauna, the atmosphere and soil. Research on the effects of fire to date has focussed primarily on the former three, whereas effects on the soil system have seen less attention. Burning and resulting post-fire environmental conditions can alter the functioning of soils physically (e.g. aggregate stability, pore size, distribution, water repellency and runoff response), chemically (e.g. nutrient availability, mineralogy, pH and C : N ratios) and biologically (e.g. biomass productivity, microbial composition and carbon sequestration). These fire-induced alterations have typically been examined in isolation by researchers in separate disciplines despite the sometimes strong relationship between some of these physical, chemical and biological effects. This special issue brings together studies from diverse disciplines, focussing on a wide spectrum of fire effects on the soil system. Here we aim to summarise, evaluate and set into context some of the new insights arising from these studies. A case is made for enhanced cross-disciplinary collaboration and the use of multi-scale research approaches in order to meet existing and future research challenges in this diverse field.

scholarly journals A study on biochemical composition of the sting gland (poison gland) and the reservoir (poison sac) of the dwarf honey bee Apis florea F. workers

2014 ◽  
Vol 6 (1) ◽  
pp. 101-105
Author(s):  
Neelima R. Kumar ◽  
Anita Devi
Keyword(s):  
Amino Acids ◽  
Alkaline Phosphatase ◽  
Honey Bee ◽  
Biochemical Composition ◽  
Free Amino ◽  
Distal Part ◽  
Wide Spectrum ◽  
Venom Gland ◽  
Apis Florea ◽  
Exocrine Glands

The glands associated with the sting apparatus of worker honey bee Apis florea produce Venom which is known to be composed of a wide spectrum of biomolecules ranging from biogenic amines to peptides and proteins. The Venom apparatus showed the presence of two important associated exocrine glands i.e. Venom gland and Dufors gland. The secretions of both glands are apocrine and are released into the lumen to be stored in the venom sac. The presence of some exocrine cells in the distal part of venom sac which is otherwise known to only store the component of Venom gland led to the present study. The present study that there were considerable differences in the biochemical composition of Venom gland and Venom sac secretions of Apis species The concentration of lipids (Sting gland =1.423±0.0001 and Reservoir = 1.21±0.0067), proteins (Sting gland=0.440±0.0226, Reservoir = 0.390± 0.032), activity of acid phosphatase (Sting gland=112.09±21.100, Reservoir=22.63±1.467) and hexokinase (Sting gland=20.7±4.016, Reservoir=10.66±2.465) was found to be more in case of Venom gland while cholesterol(Sting gland=0.138±0.0161 reservoir=0.324±0.00323), glucose (Sting gland=189±1.31, Reservoir=321±7.19), free amino acids, and activity of alkaline phosphatase (Sting gland=21.03±0.195 Reservoir=22.4±0.685) was more in Venom sac. Glycogen was absent in both Venom gland and Venom sac of Apis species as confirmed by the absence of glucose-6-phosphatase activity. It is established from the present study that Venom sac also secretes various biochemicals and enzymes which are added to the total Venom.

scholarly journals Development of a high-productivity, halophilic, thermotolerant microalga Picochlorum renovo

2019 ◽  
Vol 2 (1) ◽  
Author(s):  
Lukas R. Dahlin ◽  
Alida T. Gerritsen ◽  
Calvin A. Henard ◽  
Stefanie Van Wychen ◽  
Jeffrey G. Linger ◽  
...  
Keyword(s):  
Biomass Productivity ◽  
Downstream Processing ◽  
Culture Collection ◽  
Control Mechanisms ◽  
Tool Development ◽  
Chemical Production ◽  
Outdoor Cultivation ◽  
High Productivity ◽  
Chloroplast Genomes ◽  
Beneficial Traits

Abstract Microalgae are promising biocatalysts for applications in sustainable fuel, food, and chemical production. Here, we describe culture collection screening, down-selection, and development of a high-productivity, halophilic, thermotolerant microalga, Picochlorum renovo. This microalga displays a rapid growth rate and high diel biomass productivity (34 g m−2 day−1), with a composition well-suited for downstream processing. P. renovo exhibits broad salinity tolerance (growth at 107.5 g L−1 salinity) and thermotolerance (growth up to 40 °C), beneficial traits for outdoor cultivation. We report complete genome sequencing and analysis, and genetic tool development suitable for expression of transgenes inserted into the nuclear or chloroplast genomes. We further evaluate mechanisms of halotolerance via comparative transcriptomics, identifying novel genes differentially regulated in response to high salinity cultivation. These findings will enable basic science inquiries into control mechanisms governing Picochlorum biology and lay the foundation for development of a microalga with industrially relevant traits as a model photobiology platform.

scholarly journals Effects of Growth Medium Variation on the Nutri-Functional Properties of Microalgae Used for the Enrichment of Ricotta

2021 ◽  
Vol 60 (1) ◽  
Author(s):  
Sheyma Khemiri ◽  
Ines Bouchech ◽  
Nadia Berrejeb ◽  
Mondher Mejri ◽  
Issam Smaali ◽  
...  
Keyword(s):  
Functional Properties ◽  
Biochemical Composition ◽  
Radical Scavenging Activity ◽  
Dairy Product ◽  
Nitrogen Deficiency ◽  
Radical Scavenging ◽  
Biomass Productivity ◽  
Culture Conditions ◽  
Growth Media ◽  
Rich Media

Research background. Microalgae represent an emergent sustainable source of bioactive compounds such as antioxidants, vitamins, minerals, and polyunsaturated fatty acids that can ameliorate the nutritional characteristics of foods. The biochemical composition of microalgae could be modulated by varying the culture conditions to enhance the accumulation of biomolecules of interest. The aim of this work is to optimise the nutri-functional properties of two microalgae with potential utility for food-application. Experimental approach. Nannochloropsis gaditana.L2 and Chlorella sp.SM1 were screened for growth, biochemical composition, and radical scavenging activity employing four different growth media (Algal, BG-11, f/2, and Conway) with different nutrients composition. Additionally, the feasibility of using Chlorella sp.SM1 cultivated in BG-11 medium, in a Mediterranean under-investigated dairy product “ricotta cheese” and its effect on the sensory attributes was investigated. Results and conclusions. Nitrate- and phosphate-rich media (BG-11 and Algal) enhanced the biomass productivity. However, the highest lipid productivity (23.10 mg/(L·day); 11.86 mg/(L·day) for SM1 and L2 respectively) and carbohydrates content (34.79 %; 44.84 % for SM1 and L2 respectively) were obtained with the nitrate-deficient f/2 medium. Regardless of the used medium, the lipidic profile of Chlorella sp.SM1 and Nannochloropsis gaditana.L2 remained adequate for different applications with the presence of C16-18 as main fatty acid (>50 %). Significant increase in oleic acid (C18:1) content was recorded in response to nitrogen deficiency, being the highest in SM1 in f/2 medium (34 %). Nitrogen deficiency was also found to enhance phenolic compounds (48.8 GAE/(mg/g); 35.1 GAE/(mg/g) for SM1 and L2 respectively) and carotenoids contents (2.2 mg/g; 2 mg/g for SM1 and L2 respectively). Due to its interesting antioxidant potential, Chlorella sp.SM1 was used to enrich the ricotta cheese product at different concentrations (0.2 %, 1 % and 1.5 %). The sample with 0.2 % was found to give the most appreciated product. Novelty and scientific contribution. This study permitted the production of an innovative ricotta cheese using Chlorella, as a functional ingredient, without altering the manufacturing diagram while maintaining acceptable sensorial characteristics. The biochemical composition of the used strains varied depending on the culture media’s composition, which permitted the accumulation of phytonutrients of interest.

scholarly journals Comparison of the Binax Legionella Urinary Antigen Enzyme Immunoassay (EIA) with the Biotest Legionella Urin Antigen EIA for Detection of Legionella Antigen in both Concentrated and Nonconcentrated Urine Samples

1998 ◽  
Vol 36 (9) ◽  
pp. 2718-2722 ◽  
Author(s):  
J. A. Domínguez ◽  
N. Galí ◽  
P. Pedroso ◽  
A. Fargas ◽  
E. Padilla ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Legionella Pneumophila ◽  
No Reduction ◽  
Wide Spectrum ◽  
Cross Reactivity ◽  
Culture Collection ◽  
Urine Samples ◽  
Urinary Antigen ◽  
Antigen Present ◽  
Commercial Enzyme Immunoassay

We evaluated a newly commercial enzyme immunoassay (EIA) (BiotestLegionella Urin Antigen EIA; Biotest AG, Dreieich, Germany) for detection of antigens of all Legionella pneumophilaserogroups with a relatively wide spectrum of cross-reactivity as well as antigens of other Legionella spp. by comparing its sensitivity and specificity with those of an EIA for detection ofL. pneumophila serogroup 1 antigen (Legionella urinary antigen EIA; Binax, Portland, Maine). Both tests were performed with both concentrated and nonconcentrated urine samples. We also evaluated the capabilities of both EIAs to detect extracted soluble antigens of American Type Culture Collection (ATCC) Legionella strains (L. pneumophila serogroups 1 to 14, L. bozemanii, and L. longbeachae). The sensitivity of the Biotest EIA was 66.66% in nonconcentrated urine and 86.66% in concentrated urine. The sensitivity of the Binax EIA was 63.76% and 88.88% in nonconcentrated and concentrated urine, respectively. The specificity was 100% in nonconcentrated and concentrated urine for both assays. The Binax EIA and Biotest EIA detected extracted soluble antigens of L. pneumophila serogroups 1 to 14 and L. bozemanii ATCC strains. The cross-reactions observed with the Binax EIA were probably due to common epitopes directly related to lipopolysaccharide. Further studies are required to determine the usefulness of the Binax EIA for detection of urinary antigens fromLegionella species and serogroups other than L. pneumophila serogroup 1. The Biotest EIA proved to be as rapid, sensitive, and specific as the Binax EIA for the diagnosis of legionellosis. Concentration of antigen present in urine increased the sensitivities of both techniques with no reduction in specificity.

Characteristics of Brevibacillus laterosporus B-13186 Strain with Wide Spectrum of Antagonistic Activity

2019 ◽  
pp. 3-11
Author(s):  
N.I. Kuznetsova ◽  
M.A. Nikolaenko ◽  
O.V. Bunina ◽  
R.R. Azizbekyan
Keyword(s):  
Fusarium Solani ◽  
Wide Spectrum ◽  
Mature Spore ◽  
Phytopathogenic Fungi ◽  
Antagonistic Activity ◽  
Biochemical Characteristics ◽  
Gram Positive Bacteria ◽  
Crystalline Inclusions ◽  
Brevibacillus Laterosporus ◽  
Spore Forming Bacteria

As a result of screening of spore-forming bacteria, a B-13186 strain with a wide spectrum of antagonistic activity identified as Brevibacillus laterosporus by the analysis of sequences of variable sites of 16S rRNA was selected. Morphological, cultural and biochemical characteristics of the strain were studied. A distinctive feature of the strain is the presence of a canoe-like inclusion formed in sporangia and attached to the mature spore, as well as the ability to synthesize round-shaped crystalline inclusions. The strain was shown to be active against various species of gram-positive bacteria including S. aureus (MRSA), against phytopathogenic fungi Fusarium solani, Sclerotinia sclerotiorum, Phoma solanicola, Rhizoctonia solani and Botrytis cinerea and against microalgae Nostoc spp., Anabaena spp., Microсystis spp., Chlorella spp., Amorphonostoc spp. and Synechocystis spp. Brevibacillus laterosporus, spore-forming bacteria, microalgae, phytopathogenic fungi, crystalline inclusions.

Biochemical composition as a growth predictor in male west-coast rock lobster (Jasus lalandii)

1997 ◽  
Vol 48 (8) ◽  
pp. 845 ◽  
Author(s):  
A. C. Cockcroft
Keyword(s):  
Adult Male ◽  
Lipid Content ◽  
Biochemical Composition ◽  
High Growth ◽  
Abdominal Muscle ◽  
Growth Increment ◽  
Moult Cycle ◽  
Growth Data ◽  
Predictive Index ◽  
Size Classes

The biochemical composition of the hepatopancreas and abdominal muscle tissue of adult male Jasus lalandii in two size classes was examined on a monthly and moult-cycle basis over four years to determine the accumulation and utilization of the major reserves in these tissues. The possibility of using this information to predict moult increment, defined as the increase in carapace length, was examined. Two study areas were selected to provide contrasting information on high and low growth rates, and the annual moult increments in these areas were determined from tag-and-recapture studies. The biochemical composition of abdominal muscle did not meet the prerequisites for a predictive index of growth. Hepatopancreas moisture content (lowest values measured during accumulation of reserves) showed a negative correlation with growth increment. Peak lipid content (as both percentage and absolute values) showed a positive correlation with measured moult increment. The relationship between percentage of lipid (both size classes combined) and moult increment was highly significant. Notwithstanding the limitations introduced by the small number of high-growth data points in this study, it appears that hepatopancreas lipid content can be used as a simple and robust predictive indicator of growth in adult male J. lalandii.

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