HIF-1α Activation Reduces Expression of the microRNA hsa-miR-603 Host Gene KIAA1217 and Increases Expression of the Target CCND1 Gene in BeWo b30 Cells

2019 ◽  
Vol 35 (6) ◽  
pp. 80-86
Author(s):  
E.A. Knyazeva ◽  
S.V. Nikulin ◽  
A.Yu. Khristichenko ◽  
V.A. Petrov ◽  
A. Turchinovich ◽  
...  
Keyword(s):  
Target Gene ◽  
Ministry Of Education ◽  
Trophoblast Cells ◽  
Prolyl Hydroxylases ◽  
Ccnd1 Gene ◽  
Russian Scientific ◽  
Technological Complex ◽  
Bewo B30 ◽  
The Russian Federation ◽  
Choriocarcinoma Cell Line

The model of the placental barrier based on the human choriocarcinoma cell line BeWo b30 allows studying the effect of hypoxia on trophoblast cells. The effect of the oxyquinoline derivative inhibiting HIF-prolyl hydroxylases was studied on this model. Inhibition of these enzymes leads to an increase in the HIF-1α subunit in the cytoplasm, mimicking the cell response to hypoxia. Incubation of the cells with the drug at a concentration of 10 uM for 24 h did not affect the paracellular transport, but reduced the transport of glucose through the cell barrier. The transcriptome analysis after the exposure with oxyquinoline derivative revealed a decreased expression of the KIAA1217 gene and its intronic gene MIR603, which encodes microRNA hsa-miR-603. The expression of the target gene of this miRNA, CCND1 encoding cyclin D1, after oxyquinoline derivative exposition increased significantly, which may indicate a potential microRNA-mRNA regulatory mechanism in the response of trophoblast cells to hypoxia. BeWo b30, placenta, hypoxia, oxyquinoline, barrier, microRNA, cyclin The study was performed with the equipment of the «Postgenomic and Metabolomic Methods of Study in Molecular Biology» Common Use Center (BioClinicum Scientific and Technical Center). The study was supported by the Ministry of Education and Science of the Russian Federation in the framework of the Federal Targeted Program for Research and Development in Priority Areas of Advancement of the Russian Scientific and Technological Complex for 2014-2020 (Project no. RFMEFI58817X0007).

Laminin 521 Alters the SNAI1, ZNF708 and GRN Gene Expression in BeWo b30 Cells and Creates Physiological Conditions for the Placental Barrier

2019 ◽  
Vol 35 (5) ◽  
pp. 87-93
Author(s):  
E.N. Knyazev ◽  
T.N. Gerasimenko ◽  
O.V. Kindeeva ◽  
D.V. Maltseva ◽  
A. Turchinovich ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Cell Monolayer ◽  
Snorna Gene ◽  
Placental Barrier ◽  
Ministry Of Education ◽  
Multilayered Structures ◽  
Russian Scientific ◽  
First Case ◽  
Bewo B30 ◽  
Microrna Genes

A human choriocarcinoma cell line BeWo b30 imitates cytotrophoblast cells and thus allows to model the placental barrier. Since these cells can form multilayered structures, a method to control the number of cells based on the MTS test has been developed. It was found that during the cell growth on a laminin-521-containing extracellular matrix a significant change in the transepithelial electrical resistance (TEER) and the electrical capacitance of the cell monolayer is observed as compared to the cultivation without the extracellular matrix. The transcriptome analysis of the cells revealed that in the presence of laminin-521, the expression of the ZNF708 gene decreases and that of the SNAI1 and progranuline gene GRN increases as compared to the data obtained without the extracellular matrix. This may indicate that in the first case the cells were prepared to fuse into syncytiotrophoblast. A change in the expression of five microRNA genes and one snoRNA gene was also observed. The above mentioned effects can be associated with the used laminin. BeWo b30, placenta, barrier, microRNA, laminin 521 (laminin-11), SNAI1 The study was supported by the Ministry of Education and Science of the Russian Federation in the framework of the Federal Targeted Program for Research and Development in Priority Areas of Advancement of the Russian Scientific and Technological Complex for 2014-2020 (RFMEFI58817X0007).

Major Approaches to Assessing the Fiscal Sustainability of the Constituent Entities of the Russian Federation

2020 ◽  
Vol 26 (6) ◽  
pp. 577-583
Author(s):  
L. A. Tuaeva ◽  
I. Z. Toguzova ◽  
S. K. Tokaeva
Keyword(s):  
Russian Federation ◽  
Systems Approach ◽  
Fiscal Sustainability ◽  
Qualitative Assessment ◽  
Universal System ◽  
Russian Scientific ◽  
Analysis And Synthesis ◽  
The Russian Federation ◽  
State And Local

The presented study develops theoretical and methodological foundations for assessing the fiscal sustainability of the constituent entities of the Russian Federation in perspective.Aim. The study aims to develop a systems approach to assessing the fiscal sustainability of the constituent entities of the Russian Federation in the medium and long term.Tasks. The authors analyze the major approaches to assessing the fiscal sustainability of federal subjects and determine the significance of quantitative and qualitative assessment methods in the development of a methodology for assessing the fiscal sustainability of federal subjects in the medium and long term.Methods. This study uses scientific methods of cognition, analysis and synthesis, comparison and analogy, systems and institutional approaches to assess the fiscal sustainability of federal subjects.Results. The authors examine the major approaches to assessing the fiscal sustainability of federal subjects developed by Russian scientific schools and disciplines; approaches used by state and local authorities; approaches to assessing the fiscal sustainability of federal subjects used by international and national rating agencies; foreign experience. In general, this implies the development of a universal system of indicators for assessing the fiscal sustainability of federal subjects.Conclusions. It is substantiated that under the current conditions of new challenges, particularly in the context of the coronavirus pandemic, it is necessary to assess the long-term balance and sustainability of the budgets of federal subjects using a systems approach based on quantitative and qualitative methods, making allowance for the medium- and long-term prospects to make efficient management decisions at different levels of the economic system.

Intronic miRNAs of Apoptosis Genes as Indicators of Cell Death

2019 ◽  
Vol 35 (6) ◽  
pp. 108-113
Author(s):  
J.A. Makarova ◽  
A.A. Poloznikov
Keyword(s):  
Breast Cancer ◽  
Cancer Cell Line ◽  
Ministry Of Education ◽  
Apoptosis Induction ◽  
Cell Models ◽  
Process Stage ◽  
Mature Micrornas ◽  
The Russian Federation ◽  
Apoptosis Level ◽  
Mcf 7

A method to assess the apoptosis level in cell models based on the analysis of the expression of micRNAs located in introns of apoptosis genes has been developed. Bioinformation analysis identified 536 genes associated with apoptosis; 30 of them contained 38 pre-microRNAs encoding 41 mature microRNAs. A significant change in the expression of hsa-miR-1244 and hsa-miR-4479 in response to apoptosis induction in the MCF-7 breast cancer cell line was revealed. A correlation was also found between the expression level of these miRNAs and the size of the primary tumor (process stage) in patients with breast cancer. apoptosis, microRNA, MCF7, breast cancer This work was supported by the Ministry of Education and Science of the Russian Federation (Project no. RFMEFI61618X0092).

The Problem of Teaching Children of Immigrants in the Russian Federation

2021 ◽  
pp. 7-9
Author(s):  
Natalya Vinogradova
Keyword(s):  
Russian Federation ◽  
Children Of Immigrants ◽  
Educational Institutions ◽  
Federal State ◽  
Cultural Environment ◽  
Ministry Of Education ◽  
Interethnic Relations ◽  
Education Development ◽  
Scientific Institution ◽  
The Russian Federation

The publication proposes to discuss the problem of teaching children of immigrants who have arrived in our country and need to adapt to the new social, linguistic and cultural environment. The principles underlying the organization of education for children of immigrants in the Russian Federation, and the conditions for their education in educational institutions of our country, which were formulated at a meeting of the Council under the President of the Russian Federation for Interethnic Relations on March 30, 2021, are given. The content of the state order of the Ministry of Education of the Russian Federation to the Federal State the budgetary scientific institution “Institute of Education Development Strategy of the Russian Academy of Education” to study the difficulties faced by children of immigrants in the process of adaptation. The result of the study should be the creation of a set of guidelines for teachers organizing the work of classes in which children of immigrants study.

scholarly journals On the Needs of Specialists in Guardianship and Trusteeship Bodies for Competence Development and Methodological Support in the Constituent Entities of the Russian Federation

2021 ◽  
Vol 2 (2) ◽  
pp. 96-107
Author(s):  
V.O. Volchanskaya
Keyword(s):  
Russian Federation ◽  
State Policy ◽  
Work Experience ◽  
Training Programs ◽  
Problem Area ◽  
Advanced Training ◽  
Professional Standard ◽  
Ministry Of Education ◽  
Department Of State ◽  
The Russian Federation

The article presents an analysis of the results of monitoring to determine the need to increase the level of competence of specialists of guardianship and guardianship (OOP) (2020), provided by the Department of State Policy in the field of Protection of Children’s Rights of the Ministry of Education of the Russian Federation. In the context of the constant expansion of the powers of PLO specialists, the importance of special professional and methodological training of specialists of guardianship and guardianship authorities in relation to minors in accordance with the requirements of the professional standard and interdisciplinary competencies is evident. 406 specialists of guardianship and guardianship authorities of 85 subjects of the Russian Federation took part in the study. The analysis of 406 questionnaires of PLO specialists with different work experience in the guardianship and guardianship authorities was carried out. The average age of the PLO specialists who took part in the survey is 40 years. The gender composition of respondents is mainly represented by female specialists (92.2% of women with less than 1 year of work experience and 98.3% with more than 5 years of work experience). Representatives of executive authorities in the field of guardianship and guardianship note the need to update the existing professional standard of a specialist of guardianship and guardianship authorities in accordance with changes in state policy in the field of protection of the rights of minors. The most popular forms of receiving methodological assistance are: participation in training seminars, conferences, round tables, webinars; online consultations with experts. PLO specialists prefer to receive methodological support through telephone consultations, through various Internet resources; in the form of methodological recommendations; supervisors, business games, trainings, advanced training courses. The problem area remains the modern content of training programs, a multidisciplinary approach, and the constant expansion of the competencies necessary for the work of specialists. It is shown that for specialists with different work experience, their own principle of building advanced training programs with different volume and content of training for these categories of specialists should be implemented with the obligatory reliance on the case study.

scholarly journals University digital educational model

2020 ◽  
Vol 15 (89) ◽  
pp. 37-51
Author(s):  
Ludmila A. Aleksandrova ◽  
◽  
Edvard R. Galimov ◽  
Keyword(s):  
Russian Federation ◽  
Control Process ◽  
Digital Economy ◽  
Practical Training ◽  
Ministry Of Education ◽  
Smart Systems ◽  
Learning Path ◽  
The Russian Federation ◽  
Intelligent Methods ◽  
And Control

This work was carried out in accordance with the Digital Economy program of the Russian Federation and is therefore relevant. The program posed the following tasks for universities: the digital transformation of universities and the training of specialists of the digital economy. The Ministry of Education has articulated these issues as the creation of the Digital University. The paper proposes a model that can be laid the foundation of the Digital University. The model was developed on the basis of an analysis of the development of education in the fi of digitalization over the past 20 years, an assessment of the readiness of the leading universities of the Russian Federation to transform at present, and their own experience in using digital technologies. The proposed model satisfies the following requirements: openness of the system for students and teachers; the student’s participation in the formation of the trajectory of his own learning; the ability to access additional resources, courses and choose the places for practical training; formation and analysis of the digital trace; the use of intelligent methods for assessing acquired knowledge and competencies. The main blocks of the model are identified and the tasks for each block are formulated, which must be solved at the stage of creating the Digital University. The paper proposes approaches to their solution, technologies that the authors tested, evaluated and published the results in their previous works. In conclusion, we note that to create a Digital University, first of all, it is necessary: expand the base of control and training materials, modify, improve their forms; implement software smart systems for training and control; form a digital student trace, digital passports of information resources and, on their basis, form an individual learning path, an image of a specialist; develop learning scenarios for different images of students, goals, objectives; integrate intellectual resources of science and education; automate the management of the learning and control process as much as possible.

scholarly journals Legal and criminological analysis of the implementation of the «digital biography» of the student

2021 ◽  
pp. 90-100
Author(s):  
М.Ф. Гареев
Keyword(s):  
Information Security ◽  
Russian Federation ◽  
Digital Technology ◽  
Digital Technologies ◽  
Ministry Of Education ◽  
The Russian Federation ◽  
Criminological Analysis

В настоящей статье рассматривается инициатива Министерства просвещения Российской Федерации о введении «цифровой биографии студента». Дается правовой и криминологический анализ данной инициативы, анализируются последствия введения цифровой технологии. Специальное внимание уделяется прогнозу о росте детской преступности и возможности использования цифровых технологий в области образования криминальными элементами. Утверждается, что эти проблемы затрагивают вопросы информационной безопасности страны. This article examines the initiative of the Ministry of Education of the Russian Federation to introduce a “digital student biography”. A legal and criminological analysis of this initiative is given, the consequences of the introduction of digital technology are analyzed. Special attention is paid to the forecast of the growth of child crime and the possibility of using digital technologies in the field of education by criminals. These issues are said to affect the country's information security.

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