ANTAGONISTIC EFFECT OF Bacillus pumilus AND/OR Trichoderma viride AGAINST Fusarium solani OF COMMON BEAN

A bean (Phaseolus vulgaris) line, A - 300, resistant to Rhizoctonia solani and Fusarium oxysporum was introduced into Ontario from Colombia. The results of tests conducted in a root-rot nursery, in a greenhouse and in a growth room showed that this bean line is resistant to Fusarium solani f. sp. phaseoli and Pythium ultimum. Key words: Bean, Phaseolus vulgaris, root rot resistance

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Use of Cultural Filtrates of Certain Microbial Isolates for Powdery Mildew Control in Squash

Journal of Plant Protection Research ◽

10.2478/v10045-011-0042-8 ◽

2011 ◽

Vol 51 (3) ◽

pp. 252-260 ◽

Cited By ~ 8

Author(s):

Gaber Elkot ◽

Aly Derbalah

Keyword(s):

Powdery Mildew ◽

Trichoderma Harzianum ◽

Bacillus Pumilus ◽

Trichoderma Viride ◽

Chemical Components ◽

Yield And Quality ◽

Culture Filtrates ◽

Powdery Mildew Pathogen ◽

Microbial Isolates

Use of Cultural Filtrates of Certain Microbial Isolates for Powdery Mildew Control in SquashPowdery mildew induces significant losses in yield and quality of squash. Therefore, culture filtrates of certain microbial isolates, (Epicoccum nigrum,Epicoccum minitans,Epicoccumsp.,Trichoderma harzianum,Trichoderma virideandBacillus pumilus) were used alone, and in combination with the fungicide penconazole to control powdery mildew in squash, under field conditions. Moreover, GC-MS analysis was carried out to identify the chemical components of the most effective culture filtrates against powdery mildew pathogen. The results showed that culture filtrates of different microbial isolates (except forTrichoderma harzianum) were more effective against powdery mildew in squash than the tested fungicide alone at the recommended levels, in both tested seasons. The results also showed that mixing different culture filtrates with penconazole improved efficiency against powdery mildew compared to using the fungicide alone, in both tested seasons. The efficacy of the culture filtrates of the tested microbial isolates against powdery mildew were due to the presence of a mixture of known antifungal compounds. The results suggest the possible use of the culture filtrates of the tested microbial isolates as alternative to fungicides, in powdery mildew control. Also, this study suggests the possible mixing of the culture filtrate of the tested biocontrol agents with fungicides to minimize the applied amount of fungicides.

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Diversity and Biological Activities of Endophytic Fungi at Al-Qassim Region

Journal of Molecular Biology Research ◽

10.5539/jmbr.v9n1p160 ◽

2019 ◽

Vol 9 (1) ◽

pp. 160

Author(s):

Nahla Tharwat Elazab

Keyword(s):

Endophytic Fungi ◽

Extracellular Enzymes ◽

Biological Activities ◽

Trichoderma Viride ◽

Pathogenic Fungi ◽

Phytopathogenic Fungi ◽

Antagonistic Effect ◽

Wild Plants ◽

Dual Culture ◽

Wide Range

In recent year’s endophytic fungi has become a major concern on their host plants by enhancing their growth, increasing their fitness, strengthening their tolerances to abiotic and biotic stresses, and promoting their accumulation of secondary metabolites. Kingdom of Saudi Arabia has a wide range of flora which may be a rich source of endophytic fungi so that, the present study involves diversity and bioactivity of the endophytic fungal community in Al-Qassim region from 15 wild plants 162 isolates were obtained and identified. Among them, the most common isolates were Aspergillus niger, Aspergillus terreus, Aspergillus ochraceous and Trichoderma viride, these four endophytic isolates were examined for its antagonistic effect against six phytopathogenic fungi using two different assays, Dual-culture and Culture filtrate. Trichoderma viride recorded the most significant growth inhibition of almost pathogenic fungi followed by the three endophytic Aspergillus spp. In addition, these four endophytic fungi were screened for the production of some extracellular enzymes such as protease, cellulose, amylase, pectinase and xylanase. Our results show the ability of these isolates to produce these extracellular enzymes so this indicated the possible role of endophytic fungi as a biocontrol agent of plant disease.

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Testing Methods Affecting the Antagonistic Ability of Pseudomonas Biocontrol Strains

Agrokémia és Talajtan ◽

10.1556/agrokem.51.2002.1-2.13 ◽

2002 ◽

Vol 51 (1-2) ◽

pp. 107-114 ◽

Cited By ~ 1

Author(s):

I. Jevcsák ◽

Bálint Oldal ◽

L. Ködöböcz ◽

Keyword(s):

Rhizoctonia Solani ◽

Fusarium Solani ◽

Fungal Pathogens ◽

Culture Media ◽

Colony Growth ◽

Antagonistic Effect ◽

Bacterial Strains ◽

Testing Methods ◽

Selection Of

The antagonistic effect of thirteen Pseudomonas aeruginosa and thirteen strains of other Pseudomonas species was studied on the soil-borne phytopathogenic Rhizoctonia solani and Fusarium solani fungi. The inhibition of pathogen colony growth was tested with two different in vitro techniques using the same type of culture media. In case of the spread slant technique the antagonists induced a significantly stronger inhibition on the growth of pathogens than in case of spot transfer. Among the 26 investigated Pseudomonas strains, P. aeruginosa strains were generally more effective against the fungal pathogens. Rhizoctonia solani proved to be affected to a greater extent by the bacterial strains studied than the Fusarium solani representative. The possibility of in vitro strain selection of biocontrol microbes is being further discussed .

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Analysis of Kenyan isolates of Fusarium solani f. sp. phaseoli from common bean using colony characteristics, pathogenicity and microsatellite DNA

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb08.847 ◽

2008 ◽

Vol 7 (11) ◽

pp. 1662-1671 ◽

Cited By ~ 5

Author(s):

W Mwangrsquo ombe Agnes ◽

K Kipsumbai Pixley ◽

K Kiprop Ezekiel ◽

M Olubayo Florence ◽

W Ochieng Joel

Keyword(s):

Common Bean ◽

Fusarium Solani ◽

Microsatellite Dna

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Transcriptomic and Metabolomic Changes Triggered by Fusarium solani in Common Bean (Phaseolus vulgaris L.)

Genes ◽

10.3390/genes11020177 ◽

2020 ◽

Vol 11 (2) ◽

pp. 177

Author(s):

Limin Chen ◽

Quancong Wu ◽

Tianjun He ◽

Jianjun Lan ◽

Li Ding ◽

...

Keyword(s):

Phaseolus Vulgaris ◽

Common Bean ◽

Fusarium Solani ◽

Defense Response ◽

Fungal Pathogens ◽

Metabolite Profile ◽

Ros Generation ◽

Phaseolus Vulgaris L ◽

Cell Wall Modification ◽

Nitrogen Mobilization

Common bean (Phaseolus vulgaris L.) is a major legume and is frequently attacked by fungal pathogens, including Fusarium solani f. sp. phaseoli (FSP), which cause Fusarium root rot. FSP substantially reduces common bean yields across the world, including China, but little is known about how common bean plants defend themselves against this fungal pathogen. In the current study, we combined next-generation RNA sequencing and metabolomics techniques to investigate the changes in gene expression and metabolomic processes in common bean infected with FSP. There were 29,722 differentially regulated genes and 300 differentially regulated metabolites between control and infected plants. The combined omics approach revealed that FSP is perceived by PAMP-triggered immunity and effector-triggered immunity. Infected seedlings showed that common bean responded by cell wall modification, ROS generation, and a synergistic hormone-driven defense response. Further analysis showed that FSP induced energy metabolism, nitrogen mobilization, accumulation of sugars, and arginine and proline metabolism. Importantly, metabolic pathways were most significantly enriched, which resulted in increased levels of metabolites that were involved in the plant defense response. A correspondence between the transcript pattern and metabolite profile was observed in the discussed pathways. The combined omics approach enhances our understanding of the less explored pathosystem and will provide clues for the development of common bean cultivars’ resistant to FSP.

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Mycorrhiza consortia suppress the fusarium root rot ( Fusarium solani f. sp. Phaseoli ) in common bean ( Phaseolus vulgaris L.)

Biological Control ◽

10.1016/j.biocontrol.2016.10.001 ◽

2016 ◽

Vol 103 ◽

pp. 240-250 ◽

Cited By ~ 18

Author(s):

Pierre Eke ◽

Gael Chatue Chatue ◽

Louise Nana Wakam ◽

Rufin Marie Toghueo Kouipou ◽

Patrick Valère Tsouh Fokou ◽

...

Keyword(s):

Phaseolus Vulgaris ◽

Common Bean ◽

Fusarium Solani ◽

Root Rot ◽

Phaseolus Vulgaris L ◽

Fusarium Root Rot

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Identification of some Pathogenic Organisms Involved in Rotting of the Libyan Tirmania Truffles

Libyan Journal of Basic Sciences ◽

10.36811/ljbs.2021.110072 ◽

2021 ◽

pp. 109-116

Author(s):

Mohammed Ahmed Emhmed

Keyword(s):

Environmental Factors ◽

Ectomycorrhizal Fungi ◽

Fusarium Solani ◽

Bacillus Pumilus ◽

Fruit Body ◽

Microbial Flora ◽

Saprophytic Fungi ◽

Desert Truffle ◽

Libyan Desert ◽

Desert Truffles

Truffles are ectomycorrhizal fungi that grow symbiotically with several trees. Truffles are highly valued edible mycorrhizae fungi in economic and gastronomic terms. Desert truffles have been traditionally used as food in Libya. The rotting of truffle due to environmental factors is very common in Libya. The mechanism of truffle rot is complex and attributed mainly due to the infection with fungi, followed by bacteria. In order to document and highlight the rotting of Libyan Desert truffle phenomenon, the existence of microorganisms in the infected fruit body of Tirmania truffles has been investigated. The investigations showed changes in physical characteristics such as color, hardness, and humidity of the studied truffle samples. Also, the results showed a diversity of pathogens involved in the deterioration of truffle includes bacteria (Bacillus pumilus, Citrobacteryoungea), saprophytic fungi (Fusarium solani, Alternariachlamydosporia, and Rhizopusstolonifer). This study concluded the importance of studying microbial flora living in truffle and their environment. Keywords: Libyan Desert truffle; Tirmania; Rotting; Pathogens

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