Molecular Identification of Virulence and Antibiotic-Resistant Gene in Escherichia coli O157 and Non-O157 Recovered from Water Samples

2021 ◽  
Vol 0 (0) ◽  
pp. 0-0
Author(s):  
Busayo Olowe ◽  
Joseph Arogbodo
Keyword(s):  
Escherichia Coli ◽  
Molecular Identification ◽  
Water Samples ◽  
Escherichia Coli O157 ◽  
Antibiotic Resistant ◽  
Resistant Gene

scholarly journals Water quality assessment and plasmid analysis of multiple antibiotic-resistant Escherichia coli O157:H7 from well-water sources in Ado-Ekiti metropolis, Nigeria

2018 ◽  
Vol 19 (4) ◽  
pp. 1246-1252
Author(s):  
Jacob Olaoluwa Oluyege ◽  
Monisade Omolade Adeoye ◽  
Busayo Mutiat Olowe
Keyword(s):  
Escherichia Coli ◽  
Water Samples ◽  
Antibiotic Susceptibility ◽  
Underground Water ◽  
Water Sources ◽  
Diffusion Method ◽  
Escherichia Coli O157 ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Plasmid Analysis

Abstract This research aimed to assess the physicochemical and bacteriological quality of underground water, and determine the antibiotic susceptibility and presence of plasmids in multiple antibiotic-resistant (MAR) Escherichia coli O157:H7 in underground water sources in Ado-Ekiti. Physicochemical and bacteriological analysis of water samples were carried out using standard methods, an antibiotic susceptibility test was investigated using the standard disc diffusion method and plasmid analysis of MAR isolates was carried out using the polymerase chain reaction (PCR) technique. The physicochemical parameters analyzed were within WHO recommendations except for pH and potassium while the water samples did not conform to the WHO bacteriological recommendations for drinking water. A total of 272 E. coli were isolated and identified, among which 150 isolates were non-sorbitol fermenters (NSF) and taken as presumptive E. coli O157. MAR to three and more classes of antibiotics used were observed among these NSF with high MAR-Index, >0.2. Plasmid analysis of selected 15 isolates among the MAR NSF showed that their resistance to antibiotics was likely plasmid-mediated as they carry one to two plasmids on them. The study revealed that the water samples from Ado-Ekiti metropolis are unsafe for consumption.

scholarly journals The occurrence and sources of Campylobacter spp., Salmonellaenterica and Escherichia coli O157:H7 in the Salmon River, British Columbia, Canada

2009 ◽  
Vol 8 (2) ◽  
pp. 374-386 ◽  
Author(s):  
Cassandra C. Jokinen ◽  
Hans Schreier ◽  
William Mauro ◽  
Eduardo Taboada ◽  
Judith L. Isaac-Renton ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
British Columbia ◽  
Water Samples ◽  
Escherichia Coli O157 ◽  
Salmon River ◽  
E Coli ◽  
Faecal Samples ◽  
Precipitation Analysis ◽  
Species Markers ◽  
Pcr Techniques

In this study, we wished to assess the prevalence and determine the sources of three zoonotic bacterial pathogens (Salmonella, Campylobacter, and Escherichia coli O157:H7) in the Salmon River watershed in southwestern British Columbia. Surface water, sewage, and animal faecal samples were collected from the watershed. Selective bacterial culture and PCR techniques were used to isolate these three pathogens and indicator bacteria from these samples and characterize them. Campylobacter was the most prevalent pathogen in all samples, followed by Salmonella, and E. coli O157:H7. E. coli O157:H7 and Salmonella isolation rates from water, as well as faecal coliform densities correlated positively with precipitation, while Campylobacter isolation rates correlated negatively with precipitation. Analysis of DNA extracted from water samples for the presence of Bacteroides host-species markers, and comparisons of C. jejuniflaA-RFLP types and Salmonella serovars from faecal and water samples provided evidence that human sewage and specific domestic and wild animal species were sources of these pathogens; however, in most cases the source could not be determined or more than one source was possible. The frequent isolation of these zoonotic pathogens in the Salmon River highlights the risks to human health associated with intentional and unintentional consumption of untreated surface waters.

Effects of Acetic Acid, Lactic Acid and Trisodium Phosphate on the Microflora of Refrigerated Beef Carcass Surface Tissue Inoculated with Escherichia coli O157:H7, Listeria innocua, and Clostridium sporogenes†

1997 ◽  
Vol 60 (6) ◽  
pp. 619-624 ◽  
Author(s):  
WARREN J. DORSA ◽  
CATHERINE N. CUTTER ◽  
GREGORY R. SIRAGUSA
Keyword(s):  
Escherichia Coli ◽  
Lactic Acid ◽  
Antimicrobial Treatment ◽  
Listeria Innocua ◽  
Trisodium Phosphate ◽  
Aerobic Bacteria ◽  
Escherichia Coli O157 ◽  
Clostridium Sporogenes ◽  
Antibiotic Resistant ◽  
Beef Carcass

The microbial profiles of inoculated beef carcass tissue (BCT) were monitored during prolonged refrigerated vacuum-packaged storage following antimicrobial treatment. An industrial spray wash cabinet was used to deliver water (W), 1.5 and 3.0% lactic (LA) or acetic (AA) acid, or 12% trisodium phosphate (TSP) washes. Fresh unaltered bovine feces spiked with antibiotic-resistant strains of Escherichia coli O157:H7, Listeria innocua, and Clostridium sporogenes were used to inoculate BCT prior to all treatments. The effect of treatments on bacterial populations was tracked by monitoring levels of specific-antibiotic-resistant(marked) bacteria along with mesophilic aerobic bacteria (APC), lactic acid bacteria (LAB), and pseudomonads for up to 21 days of storage at 5°C. Initial APC levels of approximately 5.6 log CFU/cm2 were reduced by 1.3to 2.0 log CFU/cm2 by LA, AA, and TSP treatments. Marked bacteria were reduced to <1.3 log CFU/cm2, remaining that way throughout the 21-day storage. TSP treatments were not different in effectiveness from acids for controlling growth of E. coli O157:H7 and C. sporogenes, but were less effective for APC, L. innocua, or LAB. The aerobic bacteria, L. innocua, and LAB had counts ≥7 log CFU/cm2 by 7 days in all but one case and by 14 days all had counts >7 log CFU/cm2 on the untreated controls and water-washed samples. Treatments generally added a degree of safety regarding the foodborne pathogens and pathogen models used for the present study when beef tissue was stored up to 21 days and in no case did the treatments appear to offer any competitive advantage to select microorganisms on BCT.

scholarly journals Detection of Escherichia coli O157:H7 and Shiga toxin 2a gene in pork, pig feces, and clean water at Jagalan slaughterhouse in Surakarta, Central Java Province, Indonesia

2019 ◽  
Vol 12 (10) ◽  
pp. 1584-1590
Author(s):  
Maria Kristiani Epi Goma ◽  
Alvita Indraswari ◽  
Aris Haryanto ◽  
Dyah Ayu Widiasih
Keyword(s):  
Escherichia Coli ◽  
Food Safety ◽  
Shiga Toxin ◽  
Water Samples ◽  
Animal Origin ◽  
Clean Water ◽  
Escherichia Coli O157 ◽  
Fecal Samples ◽  
E Coli ◽  
Pork Samples

Background and Aim: The feasibility assessment of food products on the market becomes one of the milestones of food safety. The quality of food safety of animal origin especially pork need to get attention and more real action from the parties related and concerned. Since pork is also a source of transmission for the contagion of foodborne disease so that the study of the existence of several agents in the pork and its products become the benchmark of safety level. This study aimed to isolate, identify, and detect the Shiga toxin 2a (stx2a) gene from Escherichia coli O157:H7 in pork, pig feces, and clean water in the Jagalan slaughterhouse. Materials and Methods: A total of 70 samples consisting of 32 pork samples, 32 pig fecal samples, and 6 clean water samples were used to isolate and identify E. coli O157:H7 and the stx2a gene. Isolation and identification of E. coli O157:H7 were performed using culture on eosin methylene blue agar and Sorbitol-MacConkey agar media and confirmed molecularly with polymerase chain reaction to amplify the target genes rfbE (317 bp) and fliC (381 bp). The isolates, which were identified as E. coli O157:H7, were investigated for the stx2a gene (553 bp). Results: The results of this study show that of the total collected samples, E. coli O157:H7 was 28.6% in Jagalan slaughterhouse and consisted of 25% of pork samples, 31.25% of pig fecal samples, and 33.3% of clean water samples. The isolates that were identified to be E. coli O157:H7 mostly contained the stx2a gene, which was equal to 75%, and consisted of seven isolates from pork samples, seven isolates from fecal samples, and one isolate from clean water samples. Conclusion: E. coli O157:H7 was found in 28.6% of pork, pig feces, and clean water in Jagalan slaughterhouse and 75% of identified E. coli O157:H7 contained the stx2a gene.

Factors affecting the occurrence of Escherichia coli O157 contamination in irrigation ponds on produce farms in the Suwannee River Watershed

2013 ◽  
Vol 59 (3) ◽  
pp. 175-182 ◽  
Author(s):  
Ganyu Gu ◽  
Zhiyao Luo ◽  
Juan M. Cevallos-Cevallos ◽  
Paige Adams ◽  
George Vellidis ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Environmental Factors ◽  
Water Samples ◽  
Fecal Coliforms ◽  
Subsurface Water ◽  
Escherichia Coli O157 ◽  
Culturable Bacteria ◽  
River Watershed ◽  
E Coli ◽  
Suwannee River

Outbreaks of enteritis caused by Escherichia coli O157 associated with fresh produce have resulted in questions about the safety of irrigation water; however, associated risks have not been systematically evaluated. In this study, the occurrence and distribution of the human pathogen E. coli O157 from vegetable irrigation ponds within the Suwannee River Watershed in Georgia were investigated, and the relationship to environmental factors was analyzed. Surface and subsurface water samples were collected monthly from 10 vegetable irrigation ponds from March 2011 to February 2012. Escherichia coli O157 was isolated from enriched filtrates on CHROMagar and sorbitol MacConkey agar media and confirmed by an agglutination test. Presence of virulence genes stx1, stx2 , and eae was tested by polymerase chain reaction. In addition, 27 environmental variables of the sampled ponds were measured. Denaturing gradient gel electrophoresis was conducted for the analysis of bacterial communities in the water samples. Biserial correlation coefficients were calculated to evaluate the log10 colony-forming unit per millilitre correlations between the environmental factors and the occurrence of E. coli O157. Stepwise and canonical discriminant analyses were used to determine the factors that were associated with the presence and absence of E. coli O157 in water samples. All 10 ponds were positive for E. coli O157 some of the time, mainly in summer and fall of 2011. The temporal distribution of this bacterium differed among the 10 ponds. Temperature, rainfall, populations of fecal coliform, and culturable bacteria were positively correlated with the occurrence of E. coli O157 (P < 0.05), while the total nitrogen concentration, oxidation–reduction potential, and dissolved oxygen concentration were negatively correlated with the occurrence of this pathogen (P < 0.05). Temperature and rainfall were the most important factors contributing to the discrimination between samples with and without E. coli O157, followed by bacterial diversity and culturable bacteria population density. Bacterial numbers and diversity, including fecal coliforms and E. coli O157, increased after rainfall (and possibly runoff from pond margins) in periods with relatively high temperatures, suggesting that prevention of runoff may be important to minimize the risk of enteric pathogens in irrigation ponds.

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