The impact of addition of ascorbic acid to cryopreservation medium on dog epididymal spermatozoa

Ascorbic acid (AA) has been reported for the management of diarrhea. The anti-diarrheal potential and modulatory activities of AA on some commonly used anti-diarrheal drugs were investigated. For this purpose, the activities of AA on castor oil-induced diarrhea in Swiss mice were examined. As standard anti-diarrheal agents, we used prazosin, propranolol, loperamide, and nifedipine with or without AA. The results revealed that AA at 25 mg/kg (i.p.) and all other standard drugs exhibited significant (p < 0.05) diarrheal attenuating activities in mice. However, the impact was more pronounced in the loperamide and propranolol groups. AA administrated with prazosin and propranolol had a higher rate of latent periods and a lower rate of diarrheic secretion during the study period (4 h) than that of the other single or mixed groups. Furthermore, a molecular docking study illustrated that AA displayed good binding affinities with (α1) (–5.2 Kcal/mol), α2b (–5.4 Kcal/mol), α2c (-5.6 Kcal/mol), β1(–5.3 Kcal/mol) and β2(–6.4 Kcal/mol) adrenoceptors. Of note, AA exerted a significant anti-diarrheal effect and it was seen to modulate the anti-diarrheal effects of α- and β-adrenergic receptor blocking agents in Swiss mice.

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Effects of organic compounds on the macroalgae culture of Aegagropila linnaei

Open Chemistry ◽

10.1515/chem-2015-0119 ◽

2015 ◽

Vol 13 (1) ◽

Cited By ~ 2

Author(s):

Beata Messyasz ◽

Boguslawa Leska ◽

Joanna Fabrowska ◽

Marta Pikosz ◽

Adam Cieslak ◽

...

Keyword(s):

Ascorbic Acid ◽

Amino Acid ◽

Organic Compounds ◽

Amino Acid Content ◽

Test System ◽

Ash Content ◽

Biomass Composition ◽

Amino Acids Composition ◽

High Concentrations ◽

The Impact

AbstractThe effects of the impact of four organic compounds (ascorbic acid, biotin, glucose and sucrose) on ash, protein, fiber, fat and amino acid contents in the freshwater Aegagropila linnaei biomass were examined in 7 and 14 days of cultivations in high concentrations of tested compounds (100 mg L-1). The presence of examined organic compounds had a negligible effect on the development of algae and their biomass composition. There were no significant differences in the amino acids composition in the biomass in the presence of organic compounds compared to the test system. However, the increase in ash content was observed irrespective of the cultivation time in the case of all used organic compounds. Only slight differences in crude fat concentration were observed in the case of 7 days cultivation with ascorbic acid, biotin and sucrose, while the highest increase of ash content was observed after 14 days of supplementation with glucose. None of the compounds affected changes in amino acid content in the Aegagropila linnaei biomass. The results suggest that an environment enriched with the test organic compounds had only minimal, or at most short-term, effects on the algal biomass composition.

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Solubility and Impact of Ascorbic Acid on the in vitro Bioavailability of Two Casein-Based Iron Fortificants from Reconstituted Milk

Current Developments in Nutrition ◽

10.1093/cdn/nzaa067_063 ◽

2020 ◽

Vol 4 (Supplement_2) ◽

pp. 1836-1836

Author(s):

Magalie Sabatier ◽

Joeska Husny ◽

Marine Nicolas ◽

Stèphane Dubascoux ◽

Mary Bodis ◽

...

Keyword(s):

Ascorbic Acid ◽

Iron Uptake ◽

Iron Absorption ◽

In Vitro Digestion ◽

Iron Level ◽

Similar Amount ◽

Soluble Iron ◽

Reconstituted Milk ◽

The Impact

Abstract Objectives The two objectives were 1) to evaluate the solubility of two iron casein complexes (ICCs) under a condition mimicking gastric pH, 2) to evaluate the impact of ascorbic acid (AA) on the in vitro iron absorption of ICCs after incorporation in reconstituted whole milk powder. Methods The in vitro solubility was determined over time after addition of diluted HCl (pH 1.7), ultracentrifugation and measurement of iron appearing in the supernatant by ICP-OES (n = 2). The impact of AA on iron uptake from the Fe compounds in reconstituted milk was determined using the in vitro digestion coupled with the Caco-2 cell model and the measurement of ferritin/total protein produced by the cells (n = 3). The molar ratio of AA to iron of 2 to 1 recommended by the WHO for iron absorption optimization has been tested with an iron level corresponding to 3.3 mg Fe/serving of milk. Ferrous sulfate (FeSO4), the reference compound for iron bioavailability and micronized ferric pyrophosphate (FePP), main salt used for milk fortification were used as references. Results The dissolution test showed a rapid solubilization of iron from the ICCs i.e., >75 ± 19.3% at 5 min and >89 ± 0.3% at 90 min. The kinetics of soluble iron from the complexes were like that from FeSO4. The solubility of FePP was only 37.6 ± 4.7% at 90 min. Without AA, the iron uptake from FeSO4 was lower than expected translating into a relative in vitro bioavailability (iRBA) of FePP and of the two ICCs to FeSO4 of 66, 169 and 215%. This might be explained by a rapid conversion of soluble iron from FeSO4 into Fe3+ and insoluble iron hydroxide when the pH increased from 2 to >7 during in vitro digestion. However, with the addition of AA in the milk, iron uptake by the cells was found to be increased to levels of 341.8 ± 8.9, 124 ± 12.2, 403.1 ± 117.8 and 362.9 ± 36.9 ng ferritin/mg protein for FeSO4, FePP and the two ICCs respectively. This translates into iRBAs to FeSO4 of 36% for FePP and of 118 and 106% for the two ICCs. Conclusions The solubility and the demonstrated impact of AA on Fe uptake suggest that ICCs are absorbed to a similar amount as FeSO4 and thus provide an excellent source of Fe. Funding Sources Société des Produits Nestlé, NPTC Konolfingen, Switzerland.

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Ascorbic Acid Changes Growth of Food-Borne Pathogens in the Early Stage of Biofilm Formation

Microorganisms ◽

10.3390/microorganisms8040553 ◽

2020 ◽

Vol 8 (4) ◽

pp. 553

Author(s):

Jana Przekwas ◽

Natalia Wiktorczyk ◽

Anna Budzyńska ◽

Ewa Wałecka-Zacharska ◽

Eugenia Gospodarek-Komkowska

Keyword(s):

Ascorbic Acid ◽

Vitamin C ◽

Food Industry ◽

Early Stage ◽

Food Additives ◽

Bacterial Biofilm ◽

Biofilm Inhibition ◽

E Coli ◽

Food Borne ◽

The Impact

Since bacterial biofilm may contribute to the secondary contamination of food during the manufacturing/processing stage there is a need for new methods allowing its effective eradication. Application of food additives such as vitamin C already used in food industry as antioxidant food industry antioxidants may be a promising solution. The aim of this research was evaluation of the impact of vitamin C (ascorbic acid), in a range of concentrations 2.50 µg mL−1–25.0 mg mL−1, on biofilms of Staphylococcus aureus, Escherichia coli, and Listeria monocytogenes strains isolated from food. The efficacy of ascorbic acid was assessed based on the reduction of optical density (λ = 595 nm). The greatest elimination of the biofilm was achieved at the concentration of vitamin C of 25.0 mg mL−1. The effect of the vitamin C on biofilm, however, was strain dependent. The concentration of 25.0 mg mL−1 reduced 93.4%, 74.9%, and 40.5% of E. coli, L. monocytogenes, and S. aureus number, respectively. For E. coli and S. aureus lower concentrations were ineffective. In turn, for L. monocytogenes the biofilm inhibition was observed even at the concentration of 0.25 mg mL−1. The addition of vitamin C may be helpful in the elimination of bacterial biofilms. Nonetheless, some concentrations can induce growth of the pathogens, posing risk for the consumers’ health.

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Arsenic Trioxide with Ascorbic Acid and High-Dose Melphalan for Autologous Hematopoietic Stem Cell Transplantation for Multiple Myeloma.

Blood ◽

10.1182/blood.v108.11.3090.3090 ◽

2006 ◽

Vol 108 (11) ◽

pp. 3090-3090 ◽

Cited By ~ 1

Author(s):

Muzaffar H. Qazilbash ◽

Rima M. Saliba ◽

Marilyn S. Davis ◽

Floralyn L. Mendoza ◽

Chitra Hosing ◽

...

Keyword(s):

Multiple Myeloma ◽

Overall Survival ◽

Ascorbic Acid ◽

Arsenic Trioxide ◽

Cell Transplantation ◽

Hematopoietic Progenitor Cell ◽

High Dose ◽

Significant Difference ◽

Preparative Regimen ◽

The Impact

Abstract Backround: Arsenic trioxide (ATO) has been shown to be synergistic with melphalan both in vitro and in vivo. We conducted a phase I/II trial to determine the safety and efficacy of a combination of arsenic trioxide, melphalan and ascorbic acid (AA) as preparative regimen in patients undergoing high-dose therapy (HDT) and autologous hematopoietic progenitor cell transplantation for multiple myeloma (MM). We also assessed the impact ATO levels on melphalan pharmacokinetics (PK), engraftment and toxicity. Methods: Forty-eight patients with secretory myeloma (23 females, 25 males; median age: 54, range: 3570) were treated between 4/04 and 8/05. All patient received melphalan 100 mg/m2 IV on days -4 and -3 and AA 1000 mg/day IV on days -9 to -3. Patients were randomized to 3 arms; no ATO (arm 1), ATO 0.15 mg/kg IV on days -9 to -3 (arm 2) and ATO 0.25 mg/kg IV on days -9 to -3 (arm 3). Twelve patients had disease progression or relapse after a prior autograft. Median CD34 cells dose infused was 4.5 x 106/kg (range 2.3–10.9). Results: Patients in all 3 arms were evenly matched. With a median F/U of 14.0 months (range 6–25) post autograft, no dose-limiting toxicity or non-relapse mortality was seen. Toxicity was limited to grade I or II nausea, vomiting and diarrhea. Median ATO levels on day 0 in arms 1, 2 and 3 were 0.2, 26.3 and 46.2 ng/ml, respectively. Melphalan PK was not altered by ATO pretreatment. Median time to neutrophil engraftment (ANC >500/ dl) was 9 days. There were no engraftment failures or delays in the ATO arms. CR rate for the entire group was 23%, and total response rate (CR + PR) was 75%. 1-year Progression-free survival (PFS) and overall survival (OS) were 75% and 95%, respectively. There was no significant difference in CR, RR, PFS or OS between the 3 arms (p = 0.9, 0.9, 0.4 and 0.6, respectively). A prior autologous transplant (p = 0.02) and abnormal cytogenetics at transplant (p = 0.04) were associated with a significantly shorter remission. Conclusions: ATO + melphalan + ascorbic acid is a safe, effective and well tolerated preparative regimen for patients with multiple myeloma undergoing an autotransplant. A longer follow up is needed to assess the impact of ATO on progression-free and overall survival.

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Preliminary Evaluation of the Impact of Antioxidants Polyvinylpyrrolidone and Ascorbic Acid on Patch Budding of Persian Walnut

Journal of Horticultural Research ◽

10.1515/johr-2017-0018 ◽

2017 ◽

Vol 25 (2) ◽

pp. 19-25 ◽

Cited By ~ 1

Author(s):

Ahmadreza Abbasifar ◽

Babak Valizadehkaji

Keyword(s):

Ascorbic Acid ◽

Preliminary Evaluation ◽

Persian Walnut ◽

The Impact

AbstractThe purpose of this study is to investigate the effect of antioxidants on the budding success of three walnut genotypes under greenhouse conditions. After stratification, seeds were planted in a greenhouse at the end of autumn. After about 20 months, the seedlings grew up to 80 cm. The budding was done using scions of Z30, Z60, and B21 genotypes. Scions were immersed in polyvinylpyrrolidone (PVP) or ascorbic acid in the concentrations 2, 3, and 4 g·dm−3for 1 h before budding. Percentage of successful budding was determined after one month. The results showed that genotype significantly affected the percentage of budding success, but no interaction between genotype and treatments was found. The antioxidant solutions significantly affected all the parameters studied in this research. Ascorbic acid with 3 g·dm−3resulted in the highest percentage of budding success (86.66%); no significant differences were observed with 4 g·dm−3of ascorbic acid and 3 and 4 g·dm−3of PVP. Furthermore, antioxidant solutions significantly affected the content of chlorophylls, and 3 g·dm−3of ascorbic acid resulted in the highest content of chlorophylls in scion leaves.

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Exogenous proanthocyanidins improve tolerance of Cu-toxicity by amelioration of oxidative damage and re-programming of gene expression in Medicago sativa

PLoS ONE ◽

10.1371/journal.pone.0259100 ◽

2021 ◽

Vol 16 (10) ◽

pp. e0259100

Author(s):

Siyi Zhao ◽

Yanqiao Zhu ◽

Wenwen Liu ◽

Xiaoshan Wang ◽

Han Wang ◽

...

Keyword(s):

Oxidative Stress ◽

Cell Wall ◽

Ascorbic Acid ◽

Plant Growth ◽

Medicago Sativa ◽

Agricultural Practices ◽

Cell Wall Synthesis ◽

Genes Encoding ◽

Stress Damage ◽

The Impact

Excess copper (Cu) in soil due to industrial and agricultural practices can result in reduced plant growth. Excess Cu resulted in severely retarded root growth with severe discoloration of Alfalfa (Medicago sativa) and Medicago truncatula. Growth in the presence of hydrogen peroxide resulted in similar symptoms that could be partially recovered by the addition of the reductant ascorbic acid revealing damage was likely due to oxidative stress. The addition of proanthocyanidins (PAs) in the presence of Cu prevented much of the damage, including plant growth and restoration of lignin synthesis which was inhibited in the presence of excess Cu. Transcriptome analyses of the impact of excess Cu and the amelioration after PAs treatment revealed that changes were enriched in functions associated with the cell wall and extracellular processes, indicating that inhibition of cell wall synthesis was likely the reason for retarded growth. Excess Cu appeared to induce a strong defense response, along with alterations in the expression of a number of genes encoding transcription factors, notably related to ethylene signaling. The addition of PAs greatly reduced this response, and also induced novel genes that likely help ameliorate the effects of excess Cu. These included induction of genes involved in the last step of ascorbic acid biosynthesis and of enzymes involved in cell wall synthesis. Combined, these results show that excess Cu causes severe oxidative stress damage and inhibition of cell wall synthesis, which can be relieved by the addition of PAs.

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Influence of Antioxidants and Salinity Stress on Seed Viability Characters of Some Wheat Cultivars

Research in Plant Biology ◽

10.25081/ripb.2017.v7.3422 ◽

1970 ◽

pp. 13-20

Author(s):

A. A. Kandil, A. E. Sharief ◽

Alkhamsa K. D. Botabaah

Keyword(s):

Ascorbic Acid ◽

Humic Acid ◽

Bread Wheat ◽

Salinity Stress ◽

Seed Viability ◽

Germination Index ◽

Wheat Cultivars ◽

Germination Time ◽

Mean Germination Time ◽

The Impact

To explore the impact of antioxidants types and levels prim on seed germination characters of some bread wheat cultivars under salinity stress. An experiment accompanied in seed science lab during November and December 2016, to study the response of antioxidants seed prim of some bread wheat cultivars to germinate under salinity levels. The highest of final percentages of germination (96.8 %), higher percentages of germination energy (58.11 %), highest values of germination index (0.970) and a smaller amount of germination time (2.29 day) obtained from sown Misr 1 variety. The results showed that maximum of percentages of germination (91.15 %) and germination index (0.951) obtained from soaking in humic acid. The maximum percentages of energy of germination (41.21 %) and the less mean germination time (2.77 day from soaking grains in ascorbic acid compared with without antioxidants. Increasing antioxidant concentrations to 200 ppm produced the highest percentages of germination (91.61 %), energy of germination (37.63 %), germination index (0.953) and the lowermost of mean germination time (2.97 day) compared with without antioxidants and level of 100 ppm. Increasing salinity to 160 mM during germination of wheat cultivars significantly reduced percentages of germination by 18.5 %, energy of germination by 96.7 %, germination index by 18.6 % and mean germination time by 53.5 % compared with without salinity (control). Whereas, for reducing the gap between production and consumption, it could be recommended that soaking bread wheat in humic acid or ascorbic acid at 200 mM under salinity stress enhanced seed viability and advise to sown under saline new reclaimed soil.

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Interdisciplinary Projects Applied to Chemistry Lessons: Example of Chemistry Linked to Food Science

CHIMIA International Journal for Chemistry ◽

10.2533/chimia.2020.196 ◽

2020 ◽

Vol 74 (3) ◽

pp. 196-198

Author(s):

Lydie Moreau ◽

Véronique Breguet Mercier

Keyword(s):

Ascorbic Acid ◽

Network Formation ◽

Chemical Properties ◽

Working Environment ◽

Food Science ◽

Physico Chemical ◽

Interdisciplinary Projects ◽

The Impact ◽

Design Experiments ◽

Good Organization

An interdisciplinary project regarding the effect of ascorbic acid on bread dough's physico-chemical properties was proposed to bachelor students in chemistry and food sciences. Such an approach was proposed to develop both scientific and soft skills, in order to prepare students for their future working environment. Together, students deepened their knowledge regarding food science and chemistry. They were then able to plan and design experiments demonstrating the impact of gluten network formation and ascorbic acid influence onto bread dough and finished product characteristics. This way of teaching was very appreciated by students, nevertheless it highlighted the fact that the professors' investment was considerably high, and that good organization, alignment and preparation prior to the start of this project is key.

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