Elimination of enteroviruses, other enteric viruses, F-specific coliphages, somatic coliphages and E. coli in four sewage treatment plants of southern Germany

The objective of this work was to study the prevalence of antibiotic resistance phenotypes among total coliforms (TC), E. coli, E. faecalis and Salmonella spp. in the sewage treated effluents generated from three sewage treatment plants in Penang Malaysia. Among the isolates tested, TC and E. coli occurred high resistance for cephalexin (100 and 90.47%), ampicillin (80.93 and 95.23%) and ciprofloxacin (19.06 and 14.3%) compared to E. faecalis (42.86, 71.4 and 4.7%) and Salmonella spp. (59.8, 47.46 and 14.3%) respectively. All E. coli strains, 76.18% of TC, 66.66% of E. faecalis and 35% of Salmonella spp. were multi-resistant.

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Distribution and Relationships of Antimicrobial Resistance Determinants among Extended-Spectrum-Cephalosporin-Resistant or Carbapenem-Resistant Escherichia coli Isolates from Rivers and Sewage Treatment Plants in India

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.01950-15 ◽

2016 ◽

Vol 60 (5) ◽

pp. 2972-2980 ◽

Cited By ~ 43

Author(s):

Masato Akiba ◽

Tsuyoshi Sekizuka ◽

Akifumi Yamashita ◽

Makoto Kuroda ◽

Yuki Fujii ◽

...

Keyword(s):

Escherichia Coli ◽

Antimicrobial Resistance ◽

Sewage Treatment ◽

Indian States ◽

Content Type ◽

Sewage Treatment Plants ◽

E Coli ◽

Resistance Determinants ◽

Carbapenem Resistant ◽

Extended Spectrum

ABSTRACTTo determine the distribution and relationship of antimicrobial resistance determinants among extended-spectrum-cephalosporin (ESC)-resistant or carbapenem-resistantEscherichia coliisolates from the aquatic environment in India, water samples were collected from rivers or sewage treatment plants in five Indian states. A total of 446E. coliisolates were randomly obtained. Resistance to ESC and/or carbapenem was observed in 169 (37.9%)E. coliisolates, which were further analyzed. These isolates showed resistance to numerous antimicrobials; more than half of the isolates exhibited resistance to eight or more antimicrobials. TheblaNDMgene was detected in 14/21 carbapenem-resistantE. coliisolates:blaNDM-1in 2 isolates,blaNDM-5in 7 isolates, andblaNDM-7in 5 isolates. TheblaCTX-Mgene was detected in 112 isolates (66.3%):blaCTX-M-15in 108 isolates andblaCTX-M-55in 4 isolates. We extracted 49 plasmids from selected isolates, and their whole-genome sequences were determined. Fifty resistance genes were detected, and 11 different combinations of replicon types were observed among the 49 plasmids. The network analysis results suggested that the plasmids sharing replicon types tended to form a community, which is based on the predicted gene similarity among the plasmids. Four communities each containing from 4 to 17 plasmids were observed. Three of the four communities contained plasmids detected in different Indian states, suggesting that the interstate dissemination of ancestor plasmids has already occurred. Comparison of the DNA sequences of theblaNDM-positive plasmids detected in this study with known sequences of related plasmids suggested that various mutation events facilitated the evolution of the plasmids and that plasmids with similar genetic backgrounds have widely disseminated in India.

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Pathogenic Escherichia coli Found in Sewage Treatment Plants and Environmental Waters

Applied and Environmental Microbiology ◽

10.1128/aem.00657-12 ◽

2012 ◽

Vol 78 (16) ◽

pp. 5536-5541 ◽

Cited By ~ 49

Author(s):

E. M. Anastasi ◽

B. Matthews ◽

H. M. Stratton ◽

M. Katouli

Keyword(s):

Escherichia Coli ◽

Animal Species ◽

Sewage Treatment ◽

Environmental Waters ◽

Phylogenetic Groups ◽

Content Type ◽

Sewage Treatment Plants ◽

E Coli ◽

Nonpoint Sources ◽

Pathogenic Escherichia Coli

ABSTRACTWe previously demonstrated that someEscherichia colistrains with uropathogenic properties survived treatment stages of sewage treatment plants (STPs), suggesting that they may be released into the environment. We investigated the presence of such strains in the surrounding environmental waters of four STPs from which these persistent strains were isolated. In all, 264E. coliisolates were collected from 129 receiving water sites in a 20-km radius surrounding STPs. We also included 93E. colistrains collected from 18 animal species for comparison. Isolates were typed using a high-resolution biochemical fingerprinting method (the PhPlate system), and grouped into common (C) types. One hundred forty-seven (56%) environmental isolates were identical to strains found in STPs' final effluents. Of these, 140 (95%) carried virulence genes (VGs) associated with intestinal pathogenicE. coli(IPEC) or uropathogenicE. coli(UPEC) and were found in a variety of sites within areas sampled. Of the remaining 117 environmental strains not identical to STP strains, 105 belonged to 18 C types and 102 of them carried VGs found among IPEC or UPEC strains. These strains belonged mainly to phylogenetic groups A (A0 and A1) and B1 and to a lesser extent B22, B23, D1, and D2. Eight of 18 environmental C types, comprising 50 isolates, were also identical to bird strains. The presence of a high percentage of environmentalE. coliin waters near STPs carrying VGs associated with IPEC and UPEC suggests that they may have derived from STP effluents and other nonpoint sources.

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Occurrence of antimicrobial-resistant Escherichia coli in sewage treatment plants of South India

Journal of Water Sanitation and Hygiene for Development ◽

10.2166/washdev.2020.051 ◽

2020 ◽

Vol 10 (1) ◽

pp. 48-55 ◽

Cited By ~ 3

Author(s):

Yerabham Praveenkumarreddy ◽

Masato Akiba ◽

Keerthi Siri Guruge ◽

Keshava Balakrishna ◽

Kalwaje Eshwara Vandana ◽

...

Keyword(s):

Escherichia Coli ◽

Antimicrobial Resistance ◽

South India ◽

Sewage Treatment ◽

Domestic Wastewater ◽

Global Network ◽

Resistance Pattern ◽

Sewage Treatment Plants ◽

E Coli ◽

Significant Difference

Abstract Antibiotics received by sewage treatment plants may be the causative factor in spreading antibiotic resistance bacteria in the aquatic environment. The current study investigates the distribution of antimicrobial-resistant Escherichia coli (E. coli) in four sewage treatment plants (STPs) in South India receiving hospital and domestic wastewater in different proportions. A total of 221 E. coli isolates were checked for antimicrobial resistance against 16 antimicrobials. Among the antimicrobials tested, ampicillin (AMP) and cefazolin (CFZ) showed resistance between 20% and 90%, nalidixic acid (NAL) and ciprofloxacin (CIP) showed resistance between 15% and 75% and chloramphenicol (CHL) showed resistance between 2% and 20%. Based on the observations, there is no significant difference between the wastewater inlet and outlet, suggesting that treatment process was not effective in reducing the resistance. In conclusion, the trends of antimicrobial resistance pattern show that the levels of resistance were slightly higher in hospital wastewater than domestic wastewater. This article has been made Open Access thanks to the generous support of a global network of libraries as part of the Knowledge Unlatched Select initiative.

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Extended-spectrum β-lactamase producing Escherichia coli in hospital wastewaters and sewage treatment plants in Queensland, Australia

Canadian Journal of Microbiology ◽

10.1139/cjm-2013-0515 ◽

2013 ◽

Vol 59 (11) ◽

pp. 737-745 ◽

Cited By ~ 19

Author(s):

Aycan Gündoğdu ◽

Amy V. Jennison ◽

Helen V. Smith ◽

Helen Stratton ◽

Mohammad Katouli

Keyword(s):

Escherichia Coli ◽

Antimicrobial Agents ◽

Sewage Treatment ◽

Point Of View ◽

Hospital Wastewater ◽

Sewage Treatment Plants ◽

E Coli ◽

Extended Spectrum ◽

Inti Gene ◽

Hospital Wastewaters

We investigated the prevalence of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli in untreated hospital wastewaters and 2 sewage treatment plants (STPs). A collection of 252 ESBL-producing E. coli isolates from hospital wastewater and STPs were typed and tested for resistance to 17 antimicrobial agents and for the presence of integron-associated integrases (intI gene) and ESBL genes. Eighty-nine percent (n = 176) of the ESBL-producing E. coli strains from hospital wastewater were found in more than 1 sample (common types), with 1 common type accounting for 35% of isolates, found in all samples. These strains were also resistant to up to 9 non-β-lactam antibiotics and showed the same pattern of resistance in all samples. More than 73% of the hospital wastewater isolates possessed SHV-type ESBL as opposed to isolates from STPs that carried only CTX-M-type ESBL genes. The prevalence of the intI gene did not differ between the sources of the isolates. Certain ESBL-producing E. coli were dominant in hospital wastewaters. These strains possessed β-lactamase genes that were different from isolates found in STPs. From a public health point of view, the presence of such a high level of ESBL-producing E. coli strains in hospital wastewaters is of great importance.

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Comparison Between Chlorine Dioxide and Chlorine for Use as a Disinfectant of Wastewater Effluents

Water Science & Technology ◽

10.2166/wst.1992.0592 ◽

1992 ◽

Vol 26 (7-8) ◽

pp. 1483-1492 ◽

Cited By ~ 16

Author(s):

N. Narkis ◽

Y. Kott

Keyword(s):

Chlorine Dioxide ◽

Sewage Treatment ◽

Municipal Sewage ◽

Residual Chlorine ◽

Main Task ◽

Sewage Treatment Plants ◽

E Coli ◽

Fecal Streptococcus ◽

Environmentally Safe ◽

Effect Of Chlorine

The aim of this research was to investigate the possibility of using chlorine dioxide as an alternative to chlorine as a disinfectant of effluents from municipal sewage treatment plants, in order to obtain an environmentally safe source of water that can be reused for a variety of purposes. The main task was to compare the effect of chlorine dioxide and chlorine as a disinfectant of effluents from various types of sewage treatment plants. Residual chlorine dioxide, chlorite ion, chlorine and MPN/100mL of the surviving total coliforms, fecal colifonms, fecal streptococcus and E-coli phages were determined as a function of these disinfectants' doses and contact times.

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The occurrence and significance to animal health ofLeptospira, Mycobacterium, Escherichia coli, Brucella abortusandBacillus anthracisin sewage and sewage sludges

Journal of Hygiene ◽

10.1017/s0022172400068820 ◽

1981 ◽

Vol 86 (1) ◽

pp. 129-137 ◽

Cited By ~ 6

Author(s):

P. W. Jones ◽

Lynne M. Rennison ◽

P. R. J. Matthews ◽

P. Collins ◽

Anne Brown

Keyword(s):

Escherichia Coli ◽

Sewage Sludge ◽

Bacillus Anthracis ◽

Brucella Abortus ◽

Major Part ◽

Animal Health ◽

Sewage Treatment ◽

Sewage Effluent ◽

Sewage Treatment Plants ◽

E Coli

SUMMARYSamples of sewage, sewage sludge and sewage effluent from one or more of four sewage treatment plants were examined for the presence ofLeptospira, Mycobacterium, Escherichia coli, Brucella abortusandBacillus anthracis. Brucella abortusandBacillus anthraciswere not isolated. Eleven strains ofE. colipotentially enteropathogenic for calves or piglets, eight pathogenic strains ofMycobacteriumand one pathogenicLeptospirastrain were isolated from 101, 189 and 189 samples respectively.Sewage sludge is not considered to play a major part in the epidemiology of disease caused by these organisms.

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Sewage impact on shellfish microbial contamination

Water Science & Technology ◽

10.2166/wst.2004.0035 ◽

2004 ◽

Vol 50 (1) ◽

pp. 117-124 ◽

Cited By ~ 25

Author(s):

M. Pommepuy ◽

F. Dumas ◽

M.P. Caprais ◽

P. Camus ◽

C. Le Mennec ◽

...

Keyword(s):

Microbial Contamination ◽

Sewage Treatment ◽

Human Origin ◽

Rt Pcr ◽

Model Calculations ◽

Microbial Water Quality ◽

Sewage Treatment Plants ◽

E Coli ◽

Pcr Method ◽

The Impact

Coastal areas are frequently contaminated by microorganisms of human origin, due to high population density and low seawater renewal. To evaluate the impact of wastewater input on shellfish quality, a study was conducted in Brittany (France) over a period of 20 months. A hydrodynamic model was used to simulate wastewater impact on microbial water quality. To validate the model, wastewater from the three main sewage treatment plants and shellfish from three sites were sampled monthly. Bacterial indicators (E. coli), F-RNA phages were searched for by culture and noroviruses by RT-PCR and hybridisation. These microorganisms were detected in the three effluents and clams, with no marked seasonal variation. The microbial concentrations in the two oyster beds, distant from the effluent outfall, were low, and only three of the samples were positive for norovirus. For simulation, the winter wastewater inputs of E. coli and phages were calculated and an estimation for norovirus flux was made from the epidemic situation in the population. The microbial behaviour was included in the model by a decay-rate factor. Results from the model calculations were found to be very similar to E. coli and phage concentrations observed in shellfish. For noroviruses, the model indicated that shellfish distant from the wastewater input were under the detection limit of the RT-PCR method. This study demonstrated the use of modelisation to interpret norovirus contamination in various areas.

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Prevalence and Persistence of Escherichia coli Strains with Uropathogenic Virulence Characteristics in Sewage Treatment Plants

Applied and Environmental Microbiology ◽

10.1128/aem.00141-10 ◽

2010 ◽

Vol 76 (17) ◽

pp. 5882-5886 ◽

Cited By ~ 38

Author(s):

E. M. Anastasi ◽

B. Matthews ◽

A. Gundogdu ◽

T. L. Vollmerhausen ◽

N. L. Ramos ◽

...

Keyword(s):

Escherichia Coli ◽

Virulence Genes ◽

Sewage Treatment ◽

Subtropical Region ◽

Biochemical Phenotype ◽

Phylogenetic Groups ◽

Sewage Treatment Plants ◽

E Coli ◽

Treatment Processes ◽

Group D

ABSTRACTWe investigated the prevalence and persistence ofEscherichia colistrains in four sewage treatment plants (STPs) in a subtropical region of Queensland, Australia. In all, 264E. colistrains were typed using a high-resolution biochemical fingerprinting method and grouped into either a single or a common biochemical phenotype (S-BPT and C-BPT, respectively). These strains were also tested for their phylogenetic groups and 12 virulence genes associated with intestinal and extraintestinalE. colistrains. Comparison of BPTs at various treatment stages indicated that certain BPTs were found in two or all treatment stages. These BPTs constituted the highest proportion ofE. colistrains in each STP and belonged mainly to phylogenetic group B2 and, to a lesser extent, group D. No virulence genes associated with intestinalE. coliwere found among the strains, but 157 (59.5%) strains belonging to 14 C-BPTs carried one or more virulence genes associated with uropathogenic strains. Of these, 120 (76.4%) strains belonged to seven persistent C-BPTs and were found in all four STPs. Our results indicate that certain clonal groups ofE. coliwith virulence characteristics of uropathogenic strains can survive the treatment processes of STPs. These strains were common to all STPs and constituted the highest proportion of the strains in different treatment tanks of each STP.

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