Isolation of naphthalene-degrading bacteria from tropical marine sediments

2003 ◽  
Vol 47 (1) ◽  
pp. 303-308 ◽  
Author(s):  
W.-Q. Zhuang ◽  
J.-H. Tay ◽  
A.M. Maszenan ◽  
S.T.-L. Tay
Keyword(s):  
Marine Sediments ◽  
Oil Pollution ◽  
Environmental Concern ◽  
Enrichment Culture ◽  
Ex Situ ◽  
Bacterial Cells ◽  
Biochemical Tests ◽  
Naphthalene Degradation ◽  
Degrading Bacteria ◽  
Specific Growth Rates

Oil pollution is a major environmental concern in many countries, and this has led to a concerted effort in studying the feasibility of using oil-degrading bacteria for bioremediation. Although many oil-degrading bacteria have been isolated from different environments, environmental conditions can impose a selection pressure on the types of bacteria that can reside in a particular environment. This study reports the successful isolation of two indigenous naphthalene-degrading bacteria from oil-contaminated tropical marine sediments by enrichment culture. Strains MN-005 and MN-006 were characterized using an extensive range of biochemical tests. The 16S ribosomal deoxyribonucleic acid (rDNA) sequence analysis was also performed for the two strains. Their naphthalene degradation capabilities were determined using gas chromatography and DAPI counting of bacterial cells. Strains MN-005 and MN-006 are phenotypically and phylogenetically different from each other, and belong to the genera Staphylococcus and Micrococcus, respectively. Strains MN-005 and MN-006 had maximal specific growth rates (μmax) of 0.082 ± 0.008 and 0.30 ± 0.02 per hour, respectively, and half-saturation constants (Ks) of 0.79 ± 0.10 and 2.52 ± 0.32 mg per litre, respectively. These physiological and growth studies are useful in assessing the potential of these indigenous isolates for in situ or ex situ naphthalene pollutant bioremediation in tropical marine environments.

scholarly journals Isolation and Characterization of a Bacterial Strain Enterobacter cloacae (Accession No. KX438060.1) Capable of Degrading DDTs Under Aerobic Conditions and Its Use in Bioremediation of Contaminated Soil

2021 ◽  
Vol 14 ◽  
pp. 117863612110242
Author(s):  
Sonal Suman ◽  
Tanuja
Keyword(s):  
Contaminated Soil ◽  
Growth Parameters ◽  
Chemical Properties ◽  
Enterobacter Cloacae ◽  
Maximum Growth ◽  
Bacterial Degradation ◽  
Biochemical Tests ◽  
Isolation And Characterization ◽  
16S Rna ◽  
Degrading Bacteria

DDT is one of the most persistent pesticides among all the different types of organo-chlorine pesticides used. Among all the degradation methods, bacterial degradation of DDT is most effective. The present study was conducted to isolate different bacteria present in waste samples which have the ability to degrade DDT present in the soil in the minimum possible period of time and to observe the effect of different physical and chemical properties of the soil samples. Many pesticide degrading bacteria were isolated and identified through cultural, biochemical tests and further identified by 16S RNA sequencing method. The most potent strain DDT 1 growth in mineral salt medium supplemented with DDT as the only source of carbon (5-100 PPM) and was monitored at an optical density of 600 nm. The growth parameters at different physio-chemical conditions were further optimized. The result showed that Enterobacter cloacae had maximum growth in 15 days. FTIR analysis of the residual DDT after 15 days incubation showed that Enterobacter cloacae was able to degrade pesticide into its further metabolites of DDD, DDE, DDNU and other components can be used for biodegradation of DDT present in contaminated soil and water ecosystems.

scholarly journals Isolation and characterization of organic matter-degrading bacteria from coking wastewater treatment plant

2018 ◽  
Vol 78 (7) ◽  
pp. 1517-1524 ◽  
Author(s):  
Riqiang Li ◽  
Jianxing Wang ◽  
Hongjiao Li
Keyword(s):  
Organic Matter ◽  
Wastewater Treatment ◽  
Wastewater Treatment Plant ◽  
Removal Rate ◽  
Treatment Plant ◽  
Coking Wastewater ◽  
Naphthalene Degradation ◽  
Isolation And Characterization ◽  
Degrading Bacteria

Abstract As a step toward bioaugmentation of coking wastewater treatment 45 bacteria strains were isolated from the activated sludge of a coking wastewater treatment plant (WWTP). Three strains identified as Bacillus cereus, Pseudomonas synxantha, and Pseudomonas pseudoaligenes exhibited high dehydrogenase activity which indicates a strong ability to degrade organic matter. Subsequently all three strains showed high naphthalene degradation abilities. Naphthalene is a refractory compound often found in coking wastewater. For B. cereus and P. synxantha the maximum naphthalene removal rates were 60.4% and 79.8%, respectively, at an initial naphthalene concentration of 80 mg/L, temperature of 30 °C, pH of 7, a bacteria concentration of 15% (V/V), and shaking speed of 160 r/min. For P. pseudoaligenes, the maximum naphthalene removal rate was 77.4% under similar conditions but at 35 °C.

scholarly journals CRUDE OIL BIOREMEDIATION BY INDIGENOUS BACTERIA ISOLATED FROM OILY SLUDGE

2016 ◽  
Vol 78 (11-2) ◽  
Author(s):  
Nur Hafizah Azizan ◽  
Kasing Ak Apun ◽  
Lesley Maurice Bilung ◽  
Micky Vincent ◽  
Hairul Azman Roslan ◽  
...  
Keyword(s):  
Crude Oil ◽  
Enrichment Culture ◽  
Molecular Techniques ◽  
Oily Sludge ◽  
Minimal Salt Medium ◽  
Alkane Degradation ◽  
Growth Study ◽  
The Third ◽  
Cell Counts ◽  
Degrading Bacteria

Enrichment culture technique leads to the discovery of six presumptive TPH-degrading bacteria. Identification and characterization tests using morphological, biochemical and molecular techniques have successfully isolated Pseudomonas aeruginosa (UMAS1PF), Serratia marcescens (UMAS2SF) and Klebsiella spp. (UMAS3KF). All strains were able to use crude oil as sole carbon and energy source for their growth since they were able to survive in Minimal Salt medium supplemented with 1% (v/v) crude oil. Growth study showed that they produced the highest cell counts on the third or fourth day by 108 – 1011 CFU/ml. Six artificial consortium inoculums have been produced from the growth study. Gas chromatography analysis showed that all isolates had the ability to degrade aliphatic hydrocarbon with 100% degradation of nC19 – C24. Among the isolates, UMAS2SF was the best and fastest n-alkane degrader with degradation percentage between 55 – 90% of n-C14 – C18 in 14 days. This was followed by UMAS1PF and UMAS3KF with 11 – 82% and 1.3% degradation, respectively. Enhancement study showed that plot with inoculum and NPK addition successfully enhanced n-alkane degradation. Plot A2:B3+NPK degraded n-alkane the fastest followed by plot treated by C+NPK, A1:B2, B+NPK and A2:B3. Result showed that UMAS1PF was the best PAHs degrader as most of the high molecular weight PAHs was degraded. In the enhancement study, the plot amended with A2:B3 showed the highest PAHs degradation, followed by plots A1:B2, A3:B1:C2 and A1:C3 that was assigned as the third, fourth and fifth best in mineralizing PAHs, respectively.

scholarly journals Chromate Reduction in Serratia marcescens Isolated from Tannery Effluent and Potential Application for Bioremediation of Chromate Pollution

2002 ◽  
Vol 2 ◽  
pp. 972-977 ◽  
Author(s):  
M.A. Mondaca ◽  
V. Campos ◽  
R. Moraga ◽  
C.A. Zaror
Keyword(s):  
Natural Waters ◽  
Waste Treatment ◽  
Environmental Concern ◽  
Electron Microscopic Examination ◽  
Tannery Effluent ◽  
Chromate Reduction ◽  
Bacterial Cells ◽  
Electron Microscopic ◽  
Treatment Processes ◽  
Transmission Electron

Pollution of aquatic systems by heavy metals has resulted in increasing environmental concern because they cannot be biodegraded. One metal that gives reason for concern due to its toxicity is chromium. Cr(VI) and Cr(III) are the principal forms of chromium found in natural waters. A chromate-resistant strain of the bacterium S. marcescens was isolated from tannery effluent. The strain was able to reduce Cr(VI) to Cr(III), and about 80% of chromate was removed from the medium. The reduction seems to occur on the cell surface. Transmission electron microscopic examination of cells revealed that particles were deposited on the outside of bacterial cells. A stable biofilm was formed in less than 10 h, reaching around 1010cfu attached per milligram of activated carbon. These findings demonstrate that immobilizedS. marcescensmight be used in industrial waste treatment processes.

scholarly journals Bioutilization of Chicken Feather Wastes by Newly Isolated Keratinolytic Bacteria Into Protein Hydrolysates With Improved Functionalities

2021 ◽  
Author(s):  
Saugat Prajapati ◽  
Sushil Koirala ◽  
Anil Kumar Anal
Keyword(s):  
Amino Acid ◽  
Soluble Protein ◽  
Environmental Remediation ◽  
Amino Acid Profile ◽  
Total Soluble Protein ◽  
Feather Degradation ◽  
Biochemical Tests ◽  
Box Behnken Design ◽  
Degrading Bacteria ◽  
Chicken Farm

Abstract In this study, a novel feather-degrading bacteria B. amyloliquefaciens KB1 was isolated from chicken farm bed (CFB), identified by morphological, physico-biochemical tests followed by 16s rDNA analysis. Among observed isolates, bacterial isolate (KB1) showed the highest degree of feather degradation (74.78 ± 2.94 %) and total soluble protein (205 ± 0.03 mg/ g). Using the same species of bacteria, the optimum fermentation condition was found at 40 oC, pH 9, and 1 % (w/v) feather concentration that produced 260 mg/ g of soluble protein and 86.16 % feather degradation using response surface methodology in a Box-Behnken design space. The obtained hydrolysates exhibited bioactive properties. The amino acid profile showed the increase in concentration of essential amino acid compared with feather meal broth. The selection of safe screening source of this new bacteria in CFB produced hydrolysates with enhanced bioactivity applicable for food, feed, and cosmetic applications along with environmental remediation.

scholarly journals Extraintestinal Escherichia coli Carrying Virulence Genes in Coastal Marine Sediments

2010 ◽  
Vol 76 (17) ◽  
pp. 5659-5668 ◽  
Author(s):  
G. M. Luna ◽  
C. Vignaroli ◽  
C. Rinaldi ◽  
A. Pusceddu ◽  
L. Nicoletti ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Marine Sediments ◽  
Large Scale ◽  
Microbiological Quality ◽  
Genotypic Diversity ◽  
Coastal Sediments ◽  
Fecal Coliforms ◽  
Biochemical Tests ◽  
Term Survival ◽  
E Coli

ABSTRACT Despite the recognized potential of long-term survival or even growth of fecal indicators bacteria (FIB) in marine sediments, this compartment is largely ignored by health protection authorities. We conducted a large-scale study over approximately 50 km of the Marche coasts (Adriatic Sea) at depths ranging from 2 to 5 m. Total and fecal coliforms (FC) were counted by culture-based methods. Escherichia coli was also quantified using fluorescence in situ hybridization targeting specific 16S rRNA sequences, which yielded significantly higher abundances than culture-based methods, suggesting the potential importance of viable but nonculturable E. coli cells. Fecal coliforms displayed high abundances at most sites and showed a prevalence of E. coli. FC isolates (n = 113) were identified by API 20E, additional biochemical tests, and internal transcribed spacer-PCR. E. coli strains, representing 96% of isolates, were then characterized for genomic relatedness and phylogenetic group (A, B1, B2, and D) of origin by randomly amplified polymorphic DNA and multiplex-PCR. The results indicated that E. coli displayed a wide genotypic diversity, also among isolates from the same station, and that 44 of the 109 E. coli isolates belonged to groups B2 and D. Further characterization of B2 and D isolates for the presence of 11 virulence factor genes (pap, sfa/foc, afa, eaeA, ibeA, traT, hlyA, stx 1, stx 2, aer, and fyuA) showed that 90% of B2 and 65% of D isolates were positive for at least one of these. Most of the variance of both E. coli abundance and assemblage composition (>62%) was explained by a combination of physical-chemical and trophic variables. These findings indicate that coastal sediments could represent a potential reservoir for commensal and pathogenic E. coli and that E. coli distribution in marine coastal sediments largely depends upon the physical and trophic status of the sediment. We conclude that future sampling designs aimed at monitoring the microbiological quality of marine coastal areas should not further neglect the analysis of the sediment and that monitoring of these environments can be improved by including molecular methods as a complement of culture-based techniques.

Soil enrichment for the isolation of sporangial subgroup II Bacillus species, and observations concerning a coil-forming member of this group

1972 ◽  
Vol 18 (7) ◽  
pp. 1031-1038
Author(s):  
R. T. Wood ◽  
L. E. Casida Jr.
Keyword(s):  
Ph Value ◽  
Enrichment Culture ◽  
Divalent Cations ◽  
Soil Extract ◽  
Bacillus Species ◽  
Biochemical Tests ◽  
Soil Medium ◽  
Soil Enrichment ◽  
Subgroup Ii ◽  
Characteristic Features

Enrichment culture procedures are described which allow recovery from soil of mainly sporangial subgroup II Bacillus species, subgroup I plus subgroup II, or the latter plus a coil-forming member of subgroup II. After isolation, the coil-forming type grew normally and sporulated extensively only on agarized soil medium. Growth was normal on soil extract agar but sporulation was less extensive. Limited sporulation occurred when divalent cations were added to more conventional media. Normal vegetative growth occurred on other media investigated only when the pH value was held within relatively narrow limits. The presence of carbohydrate in agar media caused partial growth inhibition, a lack of catalase activity, and the formation of very long coiled cells plus pleomorphic cells, whereas overt cell lysis occurred in vigorously shaken broth cultures. These responses possibly reflect an unbalanced growth condition caused by growth at pH extremes, and not by carbohydrate per se. The characteristic features that set the coil-forming bacilli apart from other subgroup II Bacillus species were shown to be (1) their inability to grow at pH values below 6.5, (2) their inability to ferment carbohydrates, (3) their high oxygen requirement for growth, and (4) their ability to reduce thiosulfate to H2S. In addition, these bacilli can be distinguished from closely related established species by other biochemical tests.

scholarly journals Group-Specific Monitoring of Phenol Hydroxylase Genes for a Functional Assessment of Phenol-Stimulated Trichloroethylene Bioremediation

2001 ◽  
Vol 67 (10) ◽  
pp. 4671-4677 ◽  
Author(s):  
Hiroyuki Futamata ◽  
Shigeaki Harayama ◽  
Kazuya Watanabe
Keyword(s):  
Enrichment Culture ◽  
Pcr Primers ◽  
Amino Acid Residues ◽  
Phylogenetic Groups ◽  
Contaminated Aquifer ◽  
Consensus Sequences ◽  
Specific Pcr ◽  
Group I ◽  
Degrading Bacteria ◽  
Contrast Group

ABSTRACT The sequences of the largest subunit of bacterial multicomponent phenol hydroxylases (LmPHs) were compared. It was found that LmPHs formed three phylogenetic groups, I, II, and III, corresponding to three previously reported kinetic groups, low-K s (the half-saturation constant in Haldane's equation for trichloroethylene [TCE]), moderate-K s , and high-K s groups. Consensus sequences and specific amino acid residues for each group of LmPH were found, which facilitated the design of universal and group-specific PCR primers. PCR-mediated approaches using these primers were applied to analyze phenol/TCE-degrading populations in TCE-contaminated aquifer soil. It was found that the aquifer soil harbored diverse genotypes of LmPH, and the group-specific primers successfully amplified LmPH fragments affiliated with each of the three groups. Analyses of phenol-degrading bacteria isolated from the aquifer soil confirmed the correlation between genotype and phenotype. Competitive PCR assays were used to quantify LmPHs belonging to each group during the enrichment of phenol/TCE-degrading bacteria from the aquifer soil. We found that an enrichment culture established by batch phenol feeding expressed low TCE-degrading activity at a TCE concentration relevant to the contaminated aquifer (e.g., 0.5 mg liter−1); group II and III LmPHs were predominant in this batch enrichment. In contrast, group I LmPHs overgrew an enrichment culture when phenol was fed continuously. This enrichment expressed unexpectedly high TCE-degrading activity that was comparable to the activity expressed by a pure culture of Methylosinus trichosporium OB3b. These results demonstrate the utility of the group-specific monitoring of LmPH genes in phenol-stimulated TCE bioremediation. It is also suggested that phenol biostimulation could become a powerful TCE bioremediation strategy when bacteria possessing group I LmPHs are selectively stimulated.

scholarly journals Isolation, screening, degradation characteristics of a quinclorac-degrading bacteria D and its potential of bioremediation for rice field environment polluted by quinclorac

2021 ◽  
Author(s):  
Siqi Huang ◽  
Jiuyue PAN ◽  
Mancuo TUWANG ◽  
Hongyan LI ◽  
Chenyi MA ◽  
...  
Keyword(s):  
Rice Field ◽  
Bacterial Species ◽  
Rice Paddy ◽  
Culture Conditions ◽  
Biochemical Tests ◽  
Field Environment ◽  
Long Duration ◽  
Degrading Bacteria ◽  
Cellulosimicrobium Cellulans ◽  
Physiological And Biochemical

Quinclorac (QNC) is a highly selective, hormonal, and low-toxic herbicide with a long duration. And the growth and development of subsequent crops are easily affected by QNC accumulated in the soil. In this paper, a QNC-degrading strain D was isolated and screened from the rice paddy soil. Through morphology, physiological and biochemical tests and 16Sr DNA gene analysis, strain D was identified as Cellulosimicrobium cellulans sp. And the QNC degradation characteristics of strain D were studied. Under the optimal culture conditions, the QNC-degrading rate was 45.9% after culturing for 21 days. The QNC-degrading efficiency of strain D in the field was evaluated by a simulated pot experiment. The results show that strain D can promote the growth of rice and QNC-degrading effectively. This research could provide a new bacterial species for microbial degradation of QNC and lay a theoretical foundation for further research on QNC remediation .

scholarly journals HYDROCARBONOCLASTIC BACTERIA FROM JAKARTA BAY AND SERIBU ISLANDS

2008 ◽  
Vol 33 (1) ◽  
pp. 55-64 ◽  
Author(s):  
Yeti Darmayati ◽  
Shigeaki Harayama ◽  
Atsushi Yamazoe ◽  
Ariani Hatmanti ◽  
Sulistiani ◽  
...  
Keyword(s):  
Crude Oil ◽  
Oil Pollution ◽  
Pollution Level ◽  
Polyaromatic Hydrocarbon ◽  
Plate Method ◽  
Hydrocarbonoclastic Bacteria ◽  
The North ◽  
Water And Sediment ◽  
Oil Spillage ◽  
Degrading Bacteria

Jakarta Bay has been known as one of the most polluted marine environment in Indonesia, with no exception by oil. Seribu Islands waters, located in the north of Jakarta Bay may have been impacted by this polluted condition.It’s sometimes also hit by oil spillage from pipe leakage. The purpose of this study is to isolate and identify hydrocarbonoclastic bacteria (oil and Polyaromatic Hydrocarbon degrading bacteria) from Jakarta Bay and Seribu Island waters. The bacteria were isolated from water and sediment/sand. Isolation was prepared by enriched samples in SWP medium with Arabian Light Crude Oil (ALCO). Screening for PAH degrading bacteria has been completed by using sublimation plate method in ONR7a medium and screening for oil degrading bacteria were conducted by using oil plated method with the same medium. Bacteria identifications were done based on l6sRNA gene. The results were analyzed using BLAST and showed that 131 potential hydrocarbonoclastic bacteria have been isolated from Jakarta Bay and Seribu Island waters. Most of them were oil degrading bacteria (41.98%) and the rest were PAH degrading bacteria. Oil pollution level may impact the number of strain of hydrocarbonoclastic bacteria isolated. Among the hydrocarbonoclastic bacteria isolated from Jakarta Bay and Seribu Islands, Alcanivorax, Marinobacter, Achromobacter and Bacillus were common hydrocarbonoclastic genera in Jakarta Bay and its surrounding waters. Alcanivorax spp. is important oil and PAH-degrader found not only in temperate waters, but in tropical waters as well.

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