Membrane separation to improve degradation of road side grass by rumen enhanced solid incubation

2003 ◽  
Vol 48 (4) ◽  
pp. 163-168 ◽  
Author(s):  
R. Dalhoff ◽  
A. Rababah ◽  
V. Sonakya ◽  
N. Raizada ◽  
P.A. Wilderer
Keyword(s):  
Fatty Acids ◽  
Residence Time ◽  
Membrane Separation ◽  
Initial Permeability ◽  
Gas Pressure ◽  
Transmembrane Pressure ◽  
Membrane Unit ◽  
Complete Hydrolysis ◽  
Hydrolysis Products ◽  
Hydrolysis Of

Membrane separation proved to be an excellent means to maintain high residence time of microorganisms in an anaerobic hydrolysis reactor, and relatively low concentration of hydrolysis products. The microbial biocommunity typical for the rumen environment could be maintained, and the reactor efficiency of the reactor improved. Less than 4 days were reqired to reach almost complete hydrolysis of the grass fed into the reactor. To avoid blocking of the membrane unit, a backwash system is necessary. The membranes needed to be backwashed every 20 min with 4 bar gas-pressure for 10 s. After this treatment the initial permeability was regained. The plant was operated with a flux of 12 ml h-1cm-2 on average. The transmembrane pressure was in the range of 0.8-0.9 bar. 90% of the dissolved fatty acids permeated through the membrane.

Response Surface Methodology Optimization and Kinetic Study of Ultrafiltration-Enhanced, SCER-catalyzed Hydrolysis of Lard

2019 ◽  
Vol 17 (8) ◽  
Author(s):  
Enmin Lv ◽  
Shaoxuan Ding ◽  
Jie Lu ◽  
Zhuang Li ◽  
Lixiong Du ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Reaction Time ◽  
Response Surface ◽  
Membrane Separation ◽  
Molar Ratio ◽  
Transmembrane Pressure ◽  
Cleaning Efficiency ◽  
Membrane Cleaning ◽  
Exponential Factor ◽  
Hydrolysis Of

Abstract The integration process of polyethersulphone (PES) ultrafiltration with catalytic hydrolysis of lard was optimized by response surface methodology (RSM). The influences of molar ratio of water to lard, reaction time and transmembrane pressure on the fatty acids (FAs) yield were investigated. Results showed that the maximum FAs yield of 99.52 % was obtained under the optimized conditions of molar ratio of water to lard of 6.0:1.0, reaction time of 10.0 h and transmembrane pressure of 100.0 kPa. Moreover, the membrane cleaning efficiency was studied after four cleanings. Furthermore, the kinetic model of membrane separation process was investigated and the activation energy and pre-exponential factor were determined.

scholarly journals Enzymatic Methods for the Manipulation and Valorization of Soapstock from Vegetable Oil Refining Processes

2021 ◽  
Vol 2 (1) ◽  
pp. 74-91
Author(s):  
Beatrice Casali ◽  
Elisabetta Brenna ◽  
Fabio Parmeggiani ◽  
Davide Tessaro ◽  
Francesca Tentori
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Vegetable Oil ◽  
Lipase Production ◽  
Oil Refining ◽  
Flow Mode ◽  
Acid Oil ◽  
Industrial Level ◽  
Vegetable Oil Refining ◽  
Hydrolysis Of

The review will discuss the methods that have been optimized so far for the enzymatic hydrolysis of soapstock into enriched mixtures of free fatty acids, in order to offer a sustainable alternative to the procedure which is currently employed at the industrial level for converting soapstock into the by-product known as acid oil (or olein, i.e., free fatty acids removed from raw vegetable oil, dissolved in residual triglycerides). The further biocatalyzed manipulation of soapstock or of the corresponding acid oil for the production of biodiesel and fine chemicals (surfactants, plasticizers, and additives) will be described, with specific attention given to processes performed in continuous flow mode. The valorization of soapstock as carbon source in industrial lipase production will be also considered.

Process intensification for enzyme assisted turmeric starch hydrolysis in hydrotropic and supercritical conditions

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Yogita P. Labrath ◽  
Prafulla V. Belge ◽  
Uma G. Kulkarni ◽  
Vilas G. Gaikar
Keyword(s):  
Residence Time ◽  
Filtration Rate ◽  
Room Temperature ◽  
Curcuma Longa ◽  
Process Intensification ◽  
Enzyme Treatment ◽  
Starch Hydrolysis ◽  
Reaction Conditions ◽  
Natural Form ◽  
Hydrolysis Of

Abstract The turmeric rhizome (Curcuma longa) contains curcuminoids embedded in the starch matrix. It is thus important to target starch hydrolysis to enhance extraction of curcuminoids. In the case of starch hydrolysis, α-amylase is more efficient when the starch is in a gelatinised form than when it is in its natural form. The present work includes hydrolysis of turmeric starch in its natural and gelatinised forms using α-amylase in hydrotrope solution (HS) and scCO2. The optimum rate of starch hydrolysis was obtained using 200 IU cm−3 of α-amylase, at reaction conditions of 6.5 pH at 328 K when 10% w/w of turmeric powder was stirred at 900 rpm in HSs. The hydrolysis in 15 MPa scCO2 at room temperature required a phase modifier and 40 min of residence time (RT). The enzyme treatment of turmeric powder in HSs increased the filtration rate for curcuminoid extraction (gelatinised and native) compared to untreated turmeric powder.

Chemical Composition of Ice Containing Tephra Layers in the Byrd Station Ice Core, Antarctica

1988 ◽  
Vol 30 (3) ◽  
pp. 315-330 ◽  
Author(s):  
Julie M. Palais ◽  
Philip R. Kyle
Keyword(s):  
Chemical Composition ◽  
Silicate Glass ◽  
Residence Time ◽  
Volcanic Ash ◽  
Global Climate ◽  
Ice Core ◽  
Volcanic Eruptions ◽  
Tephra Layers ◽  
The Antarctic ◽  
Hydrolysis Of

The chemical composition of ice containing tephra (volcanic ash) layers in 22 sections of the Byrd Station ice core was examined to determine if the volcanic eruptions affected the chemical composition of the atmosphere and precipitation in the vicinity of Byrd Station. The liquid conductivity, acidity, sulfate, nitrate, aluminum, and sodium concentrations of ice samples deposited before, during, and after the deposition of the tephra layers were analyzed. Ice samples that contain tephra layers have, on average, about two times more sulfate and three to four times more aluminum than nonvolcanic ice samples. The acidity of ice samples associated with tephra layers is lowered by hydrolysis of silicate glass and minerals. Average nitrate, sodium, and conductivity are the same in all samples. Because much of the sulfur and chlorine originally associated with these eruptions may have been scavenged by ash particles, the atmospheric residence time of these volatiles would have been minimized. Therefore the eruptions probably had only a small effect on the composition of the Antarctic atmosphere and a negligible effect on local or global climate.

THE HYDROLYSIS OF MONOPHOSPHOINOSITIDE BY EXTRACTS OF BRAIN

1967 ◽  
Vol 45 (6) ◽  
pp. 853-861 ◽  
Author(s):  
W. Thompson
Keyword(s):  
Fatty Acids ◽  
Enzyme Activity ◽  
Calcium Ions ◽  
Brain Extract ◽  
Monovalent Cations ◽  
High Concentration ◽  
Diffusible Factor ◽  
Hydrolysis Of ◽  
The Brain ◽  
Long Chain

The hydrolysis of monophosphoinositide by soluble extracts from rat brain is described. Diglyceride and inositol monophosphate are liberated along with a small amount of free fatty acids. Hydrolysis of the lipid is optimal at pH 5.4 in acetate buffer. The reaction is stimulated by calcium ions or by high concentration of monovalent cations and, to a less extent, by long-chain cationic amphipathic compounds. Enzyme activity is lost on dialysis of the brain extract and can be restored by diffusible factor(s). Some differences in the conditions for hydrolysis of mono- and tri-phosphoinositides are noted.

scholarly journals Evidence for the regulation of guinea-pig heart microsomal phosphatidylcholine-hydrolysing phospholipase A1 by guanosine 5′-[γ-thio]triphosphate

1992 ◽  
Vol 288 (3) ◽  
pp. 965-968 ◽  
Author(s):  
K Badiani ◽  
X Lu ◽  
G Arthur
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Guinea Pig ◽  
Molecular Species ◽  
Inhibitory Effect ◽  
Phospholipase A1 ◽  
Guinea Pig Heart ◽  
Substrate Specificities ◽  
Rate Of Hydrolysis ◽  
Hydrolysis Of

We have recently characterized lysophospholipase A2 activities in guinea-pig heart microsomes and postulated that these enzymes act sequentially with phospholipases A1 to release fatty acids selectively from phosphatidylcholine (PC) and phosphatidylethanolamine, thus providing an alternative route to the phospholipase A2 mode of release. In a further investigation of the postulated pathway, we have characterized the PC-hydrolysing phospholipase A1 in guinea-pig heart microsomes. Our results show that the enzyme may have a preference for substrates with C16:0 over C18:0 at the sn-1 position. In addition, although the enzyme cleaves the sn-1 fatty acid, the rate of hydrolysis of PC substrates with C16:0 at the sn-1 position was influenced by the nature of the fatty acid at the sn-2 position. The order of decreasing preference was C18:2 > C20:4 = C18:1 > C16:0. The hydrolyses of the molecular species were differentially affected by heating at 60 degrees C. An investigation into the effect of nucleotides on the activity of the enzyme showed that guanosine 5′-[gamma-thio]triphosphate (GTP[S]) inhibited the hydrolysis of PC by phospholipase A1 activity, whereas GTP, guanosine 5′-[beta-thio]diphosphate (GDP[S]), GDP, ATP and adenosine 5′-[gamma-thio]triphosphate (ATP[S]) did not affect the activity. The inhibitory effect of GTP[S] on phospholipase A1 activity was blocked by preincubation with GDP[S]. A differential effect of GTP[S] on the hydrolysis of different molecular species was also observed. Taken together, the results of this study suggest the presence of more than one phospholipase A1 in the microsomes with different substrate specificities, which act sequentially with lysophospholipase A2 to release linoleic or arachidonic acid selectively from PC under resting conditions. Upon stimulation and activation of the G-protein, the release of fatty acids would be inhibited.

Rapid in vivo hydrolysis of fatty acid ethyl esters, toxic nonoxidative ethanol metabolites

1997 ◽  
Vol 273 (1) ◽  
pp. G184-G190 ◽  
Author(s):  
M. Saghir ◽  
J. Werner ◽  
M. Laposata
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Free Fatty Acids ◽  
Fatty Acid Ethyl Esters ◽  
Ethyl Esters ◽  
Ethanol Ingestion ◽  
Gi Tract ◽  
Apparent Lack ◽  
Hydrolysis Of

Fatty acid ethyl esters (FAEE), esterification products of fatty acids and ethanol, are in use as fatty acid supplements, but they also have been implicated as toxic mediators of ethanol ingestion. We hypothesized that hydrolysis of orally ingested FAEE occurs in the gastrointestinal (GI) tract and in the blood to explain their apparent lack of toxicity. To study the in vivo inactivation of FAEE by hydrolysis to free fatty acids and ethanol, we assessed the hydrolysis of FAEE administered as an oil directly into the rat stomach and when injected within the core of low-density lipoprotein particles into the circulation of rats. Our studies demonstrate that FAEE are rapidly degraded to free fatty acids and ethanol in the GI tract at the level of the duodenum with limited hydrolysis in the stomach. In addition, FAEE are rapidly degraded in the circulation, with a half-life of only 58 s. Thus the degradation of FAEE in the GI tract and in the blood provides an explanation for the apparent lack of toxicity of orally ingested FAEE.

Lipids of the fin whale (Balaenoptera physalus) from North Atlantic waters. IV. Fin whale milk

1968 ◽  
Vol 46 (3) ◽  
pp. 197-203 ◽  
Author(s):  
R. G. Ackman ◽  
C. A. Eaton ◽  
S. N. Hooper
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Total Lipid ◽  
Iodine Value ◽  
North Atlantic ◽  
Methyl Esters ◽  
Short Chain Fatty Acids ◽  
Fin Whale ◽  
Hydrolysis Of

Fatty acid compositions were determined for total lipid (17.5% of the milk and > 95% triglycerides), 2-monoglyceride obtained by enzymatic hydrolysis of isolated triglyceride, and isolated phospholipid (~1% of total lipids). The total lipid fatty acids of the milk had a composition similar to fin whale depot fat but were enriched in hexadecanoic acid and polyunsaturated fatty acids at the expense of monoethylenic acids; correspondingly the iodine value of 136 (methyl esters) was higher than the normal range (105–120) of North Atlantic fin whale blubber oils. Over 80% of the fatty acids in the 2-position of the triglycerides were accounted for by relatively short chain fatty acids, especially hexadecanoic (54.6%), tetradecanoic (13.7%), and hexadecenoic (11.2%), so that the ester iodine value was only 48. The milk phospholipids had a fatty acid composition basically similar to that of liver phospholipids (methyl ester iodine value 120) with somewhat more polyunsaturated fatty acids and accordingly an iodine value of 144 for methyl esters.

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