PHA-accumulating microorganisms in full-scale wastewater treatment plants

2008 ◽  
Vol 58 (1) ◽  
pp. 13-20 ◽  
Author(s):  
Mamoru Oshiki ◽  
Hiroyasu Satoh ◽  
Takashi Mino ◽  
Motoharu Onuki
Keyword(s):  
Activated Sludge ◽  
Staining Method ◽  
Wastewater Treatment Plants ◽  
Nile Blue ◽  
Buoyant Density ◽  
Full Scale ◽  
Oligonucleotide Probes ◽  
Microbial Groups ◽  
Activated Sludge Processes ◽  
Bacterial Groups

A study was conducted to clarify phylogenetic affiliations of polyhydroxyalkanoate (PHA)-accumulating microorganisms in full-scale activated sludge processes. Activated sludge samples obtained from three full-scale activated sludge processes were aerobically incubated with excess acetate to increase their PHA content. The buoyant density separation method was applied to selectively collect PHA-accumulating cells, which were then analysed by the group-level FISH and the PCR-DGGE-sequencing methods, and possible PHA-accumulating microbial groups were screened. A set of oligonucleotide probes targeting the microbial groups suspected to accumulate PHA was introduced, and seven oligonucleotide probes were newly designed for this purpose. PHA accumulation of probe-positive cells was confirmed by the post-FISH PHA staining method, wherein PHA staining with Nile Blue A (NBA) was applied after FISH. As a result, the following seven bacterial groups were found to have PHA: Dechloromonas, Accumulibacter, Thauera, Zoogloea, Comamonas, Competibacter and a novel cluster in Beta-proteobacteria. Based on the results of the post-FISH PHA staining method, these seven bacterial groups were estimated to account for around four-tenths to two-thirds of total PHA-accumulating microorganisms.

Upgrading Wastewater Treatment Plants with Anaerobic Selectors

1990 ◽  
Vol 22 (7-8) ◽  
pp. 35-43
Author(s):  
K. D. Tracy ◽  
S. N. Hong
Keyword(s):  
Phosphorus Removal ◽  
Wastewater Treatment Plants ◽  
Full Scale ◽  
High Rate ◽  
Nitrogen Control ◽  
Biological Phosphorus Removal ◽  
Conventional Activated Sludge ◽  
And Performance ◽  
Activated Sludge Processes ◽  
Biological Phosphorus

The anaerobic selector of the A/0™ process offers many advantages over conventional activated sludge processes with respect to process performance and operational stability. This high-rate, single-sludge process has been successfully demonstrated in full-scale operations for biological phosphorus removal and total nitrogen control in addition to BOD and TSS removal. This process can be easily utilized in upgrading existing treatment plants to meet stringent discharge limitations and to provide capacity expansion. Upgrades of two full-scale installations are described and performance data from the two facilities are presented.

An investigation into studying of the activated sludge foaming potential by using physicochemical parameters

2002 ◽  
Vol 46 (1-2) ◽  
pp. 525-528 ◽  
Author(s):  
K. Hladikova ◽  
I. Ruzickova ◽  
P. Klucova ◽  
J. Wanner
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Physicochemical Parameters ◽  
Wastewater Treatment Plants ◽  
Physicochemical Characteristics ◽  
Full Scale ◽  
Aeration Tank ◽  
Foam Formation ◽  
Individual Parameter

This paper examines how the physicochemical characteristics of the solids are related to foam formation and describes how the foaming potential of full-scale plants can be assessed. The relations among activated sludge and biological foam hydrophobicity, scum index, aeration tank cover and filamentous population are evaluated. Individual parameter comparison reveals the scumming intensity can be estimated only on the assumption that foams is already established. None of the above mentioned characteristics can be reliably used to predict the foaming episodes at wastewater treatment plants.

Rapid quantification of polyhydroxyalkanoates (PHA) concentration in activated sludge with the fluorescent dye Nile blue A

2011 ◽  
Vol 64 (3) ◽  
pp. 747-753 ◽  
Author(s):  
M. Oshiki ◽  
H. Satoh ◽  
T. Mino
Keyword(s):  
Activated Sludge ◽  
Wastewater Treatment Plants ◽  
Nile Blue ◽  
Maximum Level ◽  
Fluorescence Measurement ◽  
Batch Reactors ◽  
Floc Size ◽  
Nile Blue A ◽  
On Line ◽  
Rapid Quantification

The present study was conducted (1) to develop a rapid quantification method of polyhydroxyalkanoates (PHA) concentration in activated sludge by Nile blue A staining and fluorescence measurement and (2) to perform on-line monitoring of PHA concentrations in activated sludge. Activated sludge samples collected from laboratory scale sequencing batch reactors and full-scale wastewater treatment plants were stained with Nile blue A and their fluorescence intensities were determined. There was a high correlation (R2 > 0.97) between the fluorescence intensities of Nile blue A and PHA concentrations in activated sludge determined by gas chromatography. The Nile blue A staining and fluorescence measurement method allows us to determine PHA concentrations in activated sludge within only five minutes and up to 96 samples can be measured at once by using microplate reader. On-line monitoring of PHA concentrations in activated sludge was achieved by using a fluorometer equipped with a flow cell and the time point at which PHA concentration in activated sludge reached the maximum level could be identified. In addition, we examined the influence of pH, floc size and co-existing chemicals in activated sludge suspension on the fluorescence intensities of Nile blue A.

scholarly journals The Microbial Database for Danish wastewater treatment plants with nutrient removal (MiDas-DK) – a tool for understanding activated sludge population dynamics and community stability

2013 ◽  
Vol 67 (11) ◽  
pp. 2519-2526 ◽  
Author(s):  
A. T. Mielczarek ◽  
A. M. Saunders ◽  
P. Larsen ◽  
M. Albertsen ◽  
M. Stevenson ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Nutrient Removal ◽  
Wastewater Treatment Plants ◽  
Amplicon Sequencing ◽  
Temporal Variations ◽  
Full Scale ◽  
16S Rrna Amplicon Sequencing ◽  
Specific Factors ◽  
Operational Data

Since 2006 more than 50 Danish full-scale wastewater treatment plants with nutrient removal have been investigated in a project called ‘The Microbial Database for Danish Activated Sludge Wastewater Treatment Plants with Nutrient Removal (MiDas-DK)’. Comprehensive sets of samples have been collected, analyzed and associated with extensive operational data from the plants. The community composition was analyzed by quantitative fluorescence in situ hybridization (FISH) supported by 16S rRNA amplicon sequencing and deep metagenomics. MiDas-DK has been a powerful tool to study the complex activated sludge ecosystems, and, besides many scientific articles on fundamental issues on mixed communities encompassing nitrifiers, denitrifiers, bacteria involved in P-removal, hydrolysis, fermentation, and foaming, the project has provided results that can be used to optimize the operation of full-scale plants and carry out trouble-shooting. A core microbial community has been defined comprising the majority of microorganisms present in the plants. Time series have been established, providing an overview of temporal variations in the different plants. Interestingly, although most microorganisms were present in all plants, there seemed to be plant-specific factors that controlled the population composition thereby keeping it unique in each plant over time. Statistical analyses of FISH and operational data revealed some correlations, but less than expected. MiDas-DK (www.midasdk.dk) will continue over the next years and we hope the approach can inspire others to make similar projects in other parts of the world to get a more comprehensive understanding of microbial communities in wastewater engineering.

Aggregate size-dependence of bacterial community separation in a full-scale membrane bioreactor plant

2020 ◽  
Vol 96 (5) ◽  
Author(s):  
Shaoqing Zhang ◽  
Bing Li ◽  
Zhong Yu ◽  
Fangang Meng
Keyword(s):  
Bacterial Community ◽  
Activated Sludge ◽  
Nutrient Removal ◽  
Species Interactions ◽  
Membrane Bioreactor ◽  
Size Dependence ◽  
Full Scale ◽  
Water Separation ◽  
Significant Difference ◽  
Activated Sludge Processes

ABSTRACT The size of bacterial aggregates can determine both nutrient removal and sludge/water separation in activated sludge processes. In this study, the bacterial community structures and network associations of different sized aggregates obtained from a full-scale membrane bioreactor plant over a one-year period were investigated. Our results showed that biodiversity of larger sized aggregates was significantly higher than that of smaller ones and that the bacterial compositions of different sized aggregates differed significantly from each other. Bacteria related to nutrient removal (e.g. denitrification, hydrolysis and fermentation) were found to be significantly more abundant in larger aggregates than smaller ones. Network analysis revealed significant difference in species–species interactions, topological roles of individual OTU and keystone populations among different sized aggregates. Moreover, the occurrence of keystone OTUs affiliated with denitrifiers (Thermomonas) in networks of large and medium aggregates may suggest that denitrification influences bacterial interactions in large and medium aggregates. Overall, our results indicate the aggregates size-dependence of bacterial community separation in activated sludge. The current findings not only can provide guidance for process design and operation optimization, but also highlight the necessity for paying more attentions to the aggregate-based community, metabolic function and gene expression of activated sludge in future studies.

Practical experience with full-scale structured sheet media (SSM) integrated fixed-film activated sludge (IFAS) systems for nitrification

2014 ◽  
Vol 71 (4) ◽  
pp. 545-552 ◽  
Author(s):  
Hua Li ◽  
Jia Zhu ◽  
James J Flamming ◽  
Jack O'Connell ◽  
Michael Shrader
Keyword(s):  
Activated Sludge ◽  
Wastewater Treatment Plants ◽  
Contact Process ◽  
Plug Flow ◽  
Oxygen Demand ◽  
Cost Effective ◽  
Practical Experience ◽  
Full Scale ◽  
Fixed Film ◽  
The Usa

Many wastewater treatment plants in the USA, which were originally designed as secondary treatment systems with no or partial nitrification requirements, are facing increased flows, loads, and more stringent ammonia discharge limits. Plant expansion is often not cost-effective due to either high construction costs or lack of land. Under these circumstances, integrated fixed-film activated sludge (IFAS) systems using both suspended growth and biofilms that grow attached to a fixed plastic structured sheet media are found to be a viable solution for solving the challenges. Multiple plants have been retrofitted with such IFAS systems in the past few years. The system has proven to be efficient and reliable in achieving not only consistent nitrification, but also enhanced bio-chemical oxygen demand removal and sludge settling characteristics. This paper presents long-term practical experiences with the IFAS system design, operation and maintenance, and performance for three full-scale plants with distinct processes; that is, a trickling filter/solids contact process, a conventional plug flow activated sludge process and an extended aeration process.

Modelling and simulation of the steady-state of secondary settlers in wastewater treatment plants

2001 ◽  
Vol 43 (7) ◽  
pp. 39-46 ◽  
Author(s):  
I. Queinnec ◽  
D. Dochain
Keyword(s):  
Fluid Dynamics ◽  
Computational Fluid Dynamics ◽  
Wastewater Treatment ◽  
Steady State ◽  
Activated Sludge ◽  
Parameter Identification ◽  
Wastewater Treatment Plants ◽  
Operating Conditions ◽  
Sensitivity Studies ◽  
Activated Sludge Processes

This paper discusses the steady-state modelling of thickening in circular secondary settlers of activated sludge processes. The limitations of the solid flux theory basic models to represent steady-state operating conditions serve as a basis to introduce more sophisticated models derived from computational fluid dynamics. Parameter identification and sensitivity studies have been performed from lab-scale continuous experiments.

Oxygen transfer and uptake, nutrient removal, and energy footprint of parallel full-scale IFAS and activated sludge processes

2011 ◽  
Vol 45 (18) ◽  
pp. 5987-5996 ◽  
Author(s):  
Diego Rosso ◽  
Sarah E. Lothman ◽  
Matthew K. Jeung ◽  
Paul Pitt ◽  
W. James Gellner ◽  
...  
Keyword(s):  
Activated Sludge ◽  
Nutrient Removal ◽  
Oxygen Transfer ◽  
Full Scale ◽  
Energy Footprint ◽  
Activated Sludge Processes

scholarly journals Quantification of biologically and chemically bound phosphorus in activated sludge from full-scale plants with biological P-removal

2021 ◽  
Author(s):  
Francesca Petriglieri ◽  
Jette F. Petersen ◽  
Miriam Peces ◽  
Marta Nierychlo ◽  
Kamilla Hansen ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Mass Balance ◽  
Wastewater Treatment Plants ◽  
Raman Microspectroscopy ◽  
Full Scale ◽  
P Fractions ◽  
P Content ◽  
Total P ◽  
P Removal

AbstractLarge amounts of phosphorus (P) are present in activated sludge from municipal wastewater treatment plants, where it exists in the form of metal salt precipitates or biologically bound into the biomass as nucleic acids, cell membrane components, and the extracellular polymeric substances or, in special polyphosphate-accumulating organisms (PAOs), as intracellular polyphosphate. Only recently, methods that reliably allow an absolute quantification of the different P-fractions, such as sequential extraction, Raman microspectroscopy, solid-state 31P magic angle spinning (MAS) NMR, and solution state 31P NMR have been developed. This study combines these techniques to obtain a comprehensive P mass-balance of activated sludge from four wastewater treatment plants with enhanced biological phosphate removal (EBPR). The total content of P and various cations was measured by chemical analysis (ICP-OES), and different P fractions were extracted for chemical characterization. Chemically bound P constituted 38-69% of total P, most likely in the form of Fe, Mg, or Al minerals, while organically bound P constituted 7-9%. By using Raman microspectroscopy and solution state 31P NMR and 31P MAS NMR spectroscopy before and after anaerobic P-release experiments, poly-P was quantified and constituted 22-54% of total P in the activated sludges and was found in approx. 25% of all bacterial cells. Moreover, Raman microspectroscopy in combination with fluorescence in situ hybridization (FISH) was used to quantify the species-specific intracellular poly-P of known PAO genera (Tetrasphaera, Ca. Accumulibacter, Dechloromonas) and other microorganisms known to possess high level of poly-P, such as the filamentous Ca. Microthrix. They were all abundant, as measured by quantitative-FISH and amplicon sequencing, and accumulated large amount of poly-P, depending on their cell-size, contributing substantially to the P-removal. Interestingly, in all four EBPR plants investigated, only 1-13% of total poly-P was stored by unidentified PAO, highlighting that most PAOs in the full-scale EBPR plants investigated are now known.HighlightsExhaustive P mass-balance of main organic and inorganic P-species in four EBPR plantsQuantification of poly-P of FISH-defined PAO and other species with high P contentTotal P content was 36-50 mgP/gSS of which 31-62% was in biomass and as poly-PA high fraction of all cells (25-30%) contained a high content of poly-PKnown PAOs contained almost all poly-P in the EBPR plants investigated

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