Nitrogen transformations and mass balances in anaerobic/anoxic/aerobic batch experiments with full-scale biomasses from BNR activated sludge systems

2009 ◽  
Vol 60 (9) ◽  
pp. 2463-2470 ◽  
Author(s):  
J. Mękinia ◽  
H. D. Stensel ◽  
K. Czerwionka ◽  
J. Drewnowski ◽  
D. Zapero
Keyword(s):  
Activated Sludge ◽  
Organic Nitrogen ◽  
Full Scale ◽  
Aerobic Denitrification ◽  
Batch Reactors ◽  
Chemical Pretreatment ◽  
Mass Balances ◽  
Nitrogen Transformations ◽  
N Losses ◽  
Activated Sludge Systems

The aim of this study was to investigate nitrogen mass balances occurring inside full-scale BNR activated sludge systems, with special attention to colloidal and dissolved organic nitrogen (CON and DON) transformations. For this purpose, laboratory experiments were carried out using process biomass from two large BNR plants in northern Poland. Two parallel batch reactors were operated in a 3-phase (anaerobic/anoxic/aerobic) cycle. In one reactor, the settled wastewater without any pretreatment was used, whereas the settled wastewater after coagulation-flocculation (to remove colloidal and particulate fractions) was added to another reactor. The chemical pretreatment of settled wastewater with ZnSO4 did not adversely affect the observed nitrification rates in the (last) aerobic phase. It caused, however, a reduction of denitrification rates in the anoxic phase. Moreover, the chemical pretreatment did not appear to generally decrease DON but decreased CON. DON was explicitly produced in the aerobic phase and organic nitrogen conversion also occurred at a significant rate in the anoxic phase with biodegradable COD consumption and solids hydrolysis. The inorganic N mass balances revealed N losses up to approximately 10% which could be attributed to a few novel pathways of nitrogen removal, most likely aerobic denitrification or simultaneous nitrification/denitrification.

Study of factors affecting simultaneous nitrification and denitrification (SND)

1999 ◽  
Vol 39 (6) ◽  
pp. 61-68 ◽  
Author(s):  
Klangduen Pochana ◽  
Jürg Keller
Keyword(s):  
Carbon Source ◽  
Activated Sludge ◽  
Physical Phenomenon ◽  
Treatment Plant ◽  
Batch Reactors ◽  
Sequencing Batch Reactors ◽  
Factors Affecting ◽  
Floc Size ◽  
Sludge Flocs ◽  
Activated Sludge Systems

Experiments have been performed to gain an understanding of the conditions and processes governing the occurrence of SND in activated sludge systems. Sequencing batch reactors (SBRs) have been operated under controlled conditions using the wastewater from the first anaerobic pond in an abattoir wastewater treatment plant. Under specific circumstances, up to 95% of total nitrogen removal through SND has been found in the system. Carbon source and oxygen concentrations were found to be important process parameters. The addition of acetate as an external carbon source resulted in a significant increase of SND activity in the system. Stepwise change of DO concentration has also been observed in this study. Experiments to determine the effect of the floc size on SND have been performed in order to test the hypothesis that SND is a physical phenomenon, governed by the diffusion of oxygen into the activated sludge flocs. Initial results support this hypothesis but further experimental confirmation is still required.

scholarly journals Microbial community similarity and dissimilarity inside and across full-scale activated sludge processes for simultaneous nitrification and denitrification

2020 ◽  
Vol 81 (2) ◽  
pp. 333-344
Author(s):  
Jianfeng Wen ◽  
Mark W. LeChevallier ◽  
Wendong Tao
Keyword(s):  
Microbial Community ◽  
Dissolved Oxygen ◽  
Activated Sludge ◽  
Relative Abundance ◽  
Nitrogen Removal ◽  
Full Scale ◽  
Batch Reactors ◽  
Simultaneous Nitrification And Denitrification ◽  
Sequencing Batch Reactors ◽  
Mixed Liquor

Abstract Simultaneous nitrification and denitrification under low dissolved oxygen conditions is an energy-saving modification of the activated sludge process to achieve efficient nitrogen removal. Geographically distinct full-scale treatment plants are excellent platforms to address the links of microbial community with operating parameters. Mixed liquor samples were collected from a sequencing batch reactor plant, oxidation ditch plant, and step-feed activated sludge plant. Next-Generation Sequencing of the samples showed that the microbial communities were similar at the phylum level among the plants, being dominated by Proteobacteria. Microbial composition of functional groups was similar between the react fill and react phases of the sequencing batch reactors, among four sequencing batch reactors, and among four oxidation ditches. Nitrospira was the only identified genus of autotropic nitrifying bacteria with a relative abundance of 2.2–2.5% in the oxidation ditches and 0.4–0.7% at the other plants. Heterotrophic nitrifying–aerobic denitrifying bacteria were dominated by Dechloromonas with a relative abundance of 0.4–1.0%. Microbial community composition and nitrogen removal mechanisms were related to overall level and local zonation of dissolved oxygen, mixed liquor suspended solids concentration, nitrogen and organic loadings, and solids retention time. Low dissolved oxygen and low organic and nitrogen loadings favored growth of Nitrospira.

The Concept of Soluble Residual Product Formation in the Modelling of Activated Sludge

1989 ◽  
Vol 21 (4-5) ◽  
pp. 339-350 ◽  
Author(s):  
D. Orhon ◽  
N. Artan ◽  
Y. Cimşit
Keyword(s):  
Organic Matter ◽  
Activated Sludge ◽  
Product Formation ◽  
Cellular Materials ◽  
Batch Reactors ◽  
Sequencing Batch Reactors ◽  
Soluble Organic Matter ◽  
Residual Product ◽  
Hydrolysis Of ◽  
Activated Sludge Systems

The soluble effluent COD of a well operated activated sludge plant is likely to be composed almost entirely of organic matter generated by microbial activity. There is evidence to show that a significant portion of this soluble organic matter is non-degradable and may be due to similar microbial mechanisms. A model for the formation of these soluble residual products, (SRP), is propose'd, relating the SRP formation to the hydrolysis of non-viable cellular materials in the reactor. The set of equations describing the model are successfully calibrated and verified for a set of representative experimental data derived from Sequencing Batch Reactors. The model is found to explain and predict COD accumulation at the end of each cycle of these activated sludge systems.

Quantification of ammonia-oxidizing bacteria populations in full-scale sewage activated sludge systems and assessment of system variables affecting their performance

2006 ◽  
Vol 54 (1) ◽  
pp. 91-99 ◽  
Author(s):  
T. Limpiyakorn ◽  
F. Kurisu ◽  
O. Yagi
Keyword(s):  
Activated Sludge ◽  
Real Time ◽  
Real Time Pcr ◽  
Full Scale ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Ammonia Oxidizing Bacteria ◽  
Primer Sets ◽  
Activated Sludge Systems ◽  
Pcr Primer

This study carried out quantification of ammonia-oxidizing bacteria (AOB) populations in 12 full-scale sewage activated sludge systems that were different in ammonia removals and treatment processes during three different seasons. Experiment was divided into 3 parts: 1) analysis of AOB communities by PCR-DGGE-cloning-sequencing of 16S rRNA genes; 2) development of four real-time PCR primer sets for quantification of the particular AOB of interest; and 3) quantification of AOB populations by using the newly developed real-time PCR primer sets. The results suggested that all the primer sets gave good reproducibility and specificity for PCR amplification with the detection limits of 102 copies/PCR reaction. Although the 12 systems were different in several aspects, one of the identified sequence types of Nitrosomonas oligotropha cluster was the dominant AOB in every system and every season studied. However, the other sequence type of this cluster was not significantly involved in ammonia removals in the systems. The occurrence of N. communis cluster in the systems seemed to depend on the remaining oxygen concentrations in the sludge floc and thus the activity of aerobic heterotrophs in the aeration tanks. N. europaea–Nitrosococcus. mobilis solely existed in one A2O system of which the influent contained twice the chloride concentrations than those of other systems.

Incidence of Sphaerotilus Natans in Laboratory Scale Activated Sludge Systems

1989 ◽  
Vol 21 (4-5) ◽  
pp. 29-41 ◽  
Author(s):  
D. M. D. Gabb ◽  
G. A. Ekama ◽  
D. Jenkins ◽  
G. v. R. Marais
Keyword(s):  
Activated Sludge ◽  
Surface Area ◽  
Volume Ratio ◽  
Full Scale ◽  
Laboratory Scale ◽  
Sphaerotilus Natans ◽  
Attached Growth ◽  
Mixed Liquor ◽  
Activated Sludge Systems

Bulking in activated sludge systems due to proliferation of Sphaerotilus natans is very common in laboratory-scale but rare in full-scale systems. From two laboratory-scale studies it is concluded that a cause for proliferation was attached growth of S.natans on the walls of the feed lines and reactor surfaces continuously seeding the mixed liquor. It is suggested that S.natans bulking in laboratory-scale systems is common compared to full-scale systems because the surface area/volume ratio of the former is orders of magnitude higher than that of the latter so that the potential for seeding from attached growths in laboratory-scale plants is correspondingly higher. It would appear that in laboratory-scale activated sludge systems regular cleaning of the feed lines and daily scrubbing of the reactor and other wetted surfaces will eliminate S.natans bulking due to seeding of the mixed liquor from these surfaces.

Implementation of glycogen accumulating bacteria in treating nutrient-deficient wastewater

2002 ◽  
Vol 46 (1-2) ◽  
pp. 185-190 ◽  
Author(s):  
A. Jobbágy ◽  
B. Literáthy ◽  
G. Tardy
Keyword(s):  
Activated Sludge ◽  
Analytical Data ◽  
Carbon Sources ◽  
Experimental System ◽  
Full Scale ◽  
Extracellular Polysaccharides ◽  
Excess Carbon ◽  
Biomass Structure ◽  
Scale Experiment ◽  
Activated Sludge Systems

Activated sludge treatment of nutrient-deficient wastes may lead to severe slime formation and consequent biomass separation difficulties. The purpose of this paper has been to show that bioreactor arrangement essentially influences the manner of biological excess carbon removal. In a comparative lab-scale experiment two differently arranged activated sludge systems were operated simultaneously: an aerated CSTR with an aerobic selector and an aerated CSTR with an anaerobic selector. The seed derived from an anaerobic/aerobic activated sludge plant of a winery. The model wastewater contained wine, sugar and acetic acid as organic carbon sources and lacked nutrients regarding both N and P, similarly to the influent of the full-scale plant. During the 52 days of the experiment the SVI values of the fully aerated system increased up to 600–800 cm3 g−1 whereas those of the anaerobic/aerobic system remained below 250 cm3 g−1. The SVI values showed a strict correlation with the amount of extracellular polysaccharides. In the anaerobic/aerobic experimental system, the high (40% of MLSS) intracellular polysaccharide content of the seed could be maintained. Besides the analytical data, also the microscopic observations of the biomass structure referred to the presence of glycogen accumulating organisms in both the lab- and full-scale anaerobic/aerobic systems.

scholarly journals Removal of bacterial and viral indicator organisms in full-scale aerobic granular sludge and conventional activated sludge systems

2020 ◽  
Vol 6 ◽  
pp. 100040 ◽  
Author(s):  
Mary Luz Barrios-Hernández ◽  
Mario Pronk ◽  
Hector Garcia ◽  
Arne Boersma ◽  
Damir Brdjanovic ◽  
...  
Keyword(s):  
Activated Sludge ◽  
Granular Sludge ◽  
Full Scale ◽  
Aerobic Granular Sludge ◽  
Indicator Organisms ◽  
Conventional Activated Sludge ◽  
Activated Sludge Systems
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