scholarly journals Clinical signs in Japanese quails (Coturnix coturnix) experimentally infected with Salmonella Gallinarum

2016 ◽  
Vol 10 (3) ◽  
pp. 253 ◽  
Author(s):  
Roberta Cristina da Rocha e Silva ◽  
William Maciel Cardoso ◽  
Régis Siqueira de Castro Teixeira ◽  
Camila Muniz Cavalcante ◽  
Clarice Pessoa Almeida ◽  
...  
Keyword(s):  
Clinical Signs ◽  
Ovarian Follicles ◽  
Control Group ◽  
Microbiological Analysis ◽  
Salmonella Gallinarum ◽  
Coturnix Coturnix ◽  
Fowl Typhoid ◽  
Japanese Female ◽  
And Control ◽  
Splenic Necrosis

Fowl typhoid a high-impact disease in the poultry industry, this study aims to verify the clinical signs of the disease in experimentally inoculated with quail Salmonella Gallinarum 54 Japanese female quails were distributed into two groups. The inoculated group (GI) SG (32 quail) and control group (CG) (16 birds). A total of six birds were euthanized before the start of the experiment, organs samples (liver, spleen, ovarian follicles, cecum and lung) were collected for the microbiological analysis. The birds of the GI group received 0,7mL of inoculum (1.5x106 CFU/mL) and birds of the CG group received 0,7 mL of saline solution. Thereafter, the quails were observed for ten consecutive days in three daily periods (8:00, 11:00 e 14:00) in order to observe clinical signs of disease. After four days of inoculation was verified prostration, apathy, ruffled feathers, diarrhea, bird's stay in the corner of the cage with closed eyes and reluctance to move (13/32). Some macroscopic changes were observed in euthanized birds with clinical signs like splenomegaly and splenic necrosis in 84,6% (11/13) and 23,0% (3/13) respectively, hepatomegaly and liver necrosis in 15,4% (2/13) e 23% (3/13) respectively, and atrophy and hemorrhage of ovarian follicles in 15,4% (2/13) and 7,7% (1/13) respectively. the birds that died was observed only hepatosplenomegaly. So we can conclude experimentally inoculated with Salmonella Gallinarum quail have the typical clinical signs of fowl typhoid observed in others birds of Galliforme order.

scholarly journals Relationship between clinical symptomatology on the isolation of Salmonella Gallinarum of japanese quail (Coturnix coturnix)

2018 ◽  
Vol 70 (4) ◽  
pp. 1187-1194
Author(s):  
R.C. Rocha-e-Silva ◽  
W.M. Cardoso ◽  
R.V. Horn ◽  
C.M. Cavalcanti ◽  
A.J.F. Beleza ◽  
...  
Keyword(s):  
Japanese Quail ◽  
High Mortality ◽  
Clinical Signs ◽  
Ovarian Follicles ◽  
Control Group ◽  
Salmonella Gallinarum ◽  
Coturnix Coturnix ◽  
Clinical Symptomatology ◽  
Eyes Closed ◽  
Japanese Quails

ABSTRACT Salmonella Gallinarum is capable of causing high mortality in birds of the order Galliformes. This study aimed to relate the presence of clinical signs with the recovery of Salmonella Gallinarum from organs and c loacal swabs of Japanese quails (Coturnix coturnix) experimentally infected. A total of 70 female quails were housed in a pair per cage and divided in two groups (IG: quails inoculated with 1.5x106 CFU of Salmonella Gallinarum Nalr/mL and CG: control group). After the inoculation, birds were evaluated three times a day to verify the presence of clinical signs. Birds that presented ruffled feathers, eyes closed and remained quiet in the cage were removed for euthanasia, as well as the same number of birds from the inoculated groups that presented no clinical signs and from the control group. Cloacal swabbing was performed following euthanasia for the sampling of liver, spleen, caeca, ovarian follicles and lung for microbiological procedure. Quails with clinical signs and quails found dead presented positivity of 100%. While inoculated quails with no clinical signs presented a lower positivity (38.5%). Therefore, quails with septicemia caused by SG present clinical signs of the disease and the pathogen can be isolated and quantified in the organs.

scholarly journals Experimental infection of broiler chicks with Salmonella Typhimurium from pigeon (Columba livia)

2016 ◽  
Vol 37 (4) ◽  
pp. 1919
Author(s):  
Átilla Holanda de Albuquerque ◽  
Régis Siqueira de Castro Teixeira ◽  
Débora Nishi Machado ◽  
Elisângela De Souza Lopes ◽  
Ruben Horn Vasconcelos ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Clinical Signs ◽  
Columba Livia ◽  
Poultry Feed ◽  
Economic Losses ◽  
Control Group ◽  
Broiler Chicks ◽  
Post Inoculation ◽  
Microbiological Analysis ◽  
Fecal Samples

Several cases of animal and human salmonellosis caused by the Salmonella serotype Typhimurium have been reported. In animals, subclinical infection favors pathogen dissemination through feces. In this context, the domestic pigeon (Columba livia) with an asymptomatic condition may play an important role in the transmission of salmonellosis, through the elimination of contaminated feces in commercial aviaries or in poultry feed facilities, causing economic losses to the poultry industry and presenting a risk to public health. This study aimed to evaluate the mortality, clinical signs and the presence of Salmonella Typhimurium in the feces and organs of chicks previously inoculated with bacteria isolated from a pigeon. One-day-old chicks were distributed in two experimental groups (G1 and G2) of 32 birds each, and a control group of six birds. Two inocula of 0.4 and 0.7 mL with 105 and 106 colony forming units were used in G1 and G2 birds, respectively. At 1, 4, 7 and 14 days post-inoculation (dpi) fecal samples were pooled from each cage and individual cloacal swabs were collected. At 14 dpi, all chicks were euthanized and samples were collected from the liver, spleen, lung, cecum and intestine for microbiological analysis. Mortality was only observed among G2 birds (6.25%). Most birds presented clinical signs of diarrhea at 4 dpi and no symptom as observed at 14 dpi. The results from cloacal swabs demonstrated bacterial elimination in 68.8% and 53.1% of G2 and G1 birds, respectively at 1 dpi. Additionally, fecal samples had elevated bacterial shedding in all four periods of observation , with a higher excretion at 4 dpi (62.5%) for both groups. Among G2 birds, 74.2% were positive for the pathogen in the intestine; G1 birds presented the lowest rate of lung infection (29%), and both groups had more than 50% positivity for liver and caeca. The results revealed that infected chicks with a Salmonella Typhimurium strains isolated from pigeons may host the pathogen in several organs, and simultaneously present diarrheic disorders with significant levels of bacterial excretion in feces.

MONIZEN FORTE FOR LAMBS PARASIT-IC DISEASES

2020 ◽  
Vol 2 ◽  
pp. 84-87
Author(s):  
E. S. Engasheva ◽  
◽  
V. I. Kolesnikov ◽  
Keyword(s):  
Clinical Signs ◽  
Live Weight ◽  
Control Group ◽  
Not Given ◽  
The North ◽  
Condi Tions ◽  
High Infection ◽  
Mucous Discharge ◽  
And Control ◽  
Experimental Group

The Monizen Forte test for parasitic sheep diseases was tested under production condi-tions on 460 lambs of the North Caucasian breed, with a live weight of 25-30 kg, with high infection with nematodes, cestodes and larvae of the nasopharyngeal gadfly (Oestrus ovis), which were given orally in a mixture with half the normal rate of concentrated feed, the test drug in a dose of 1 ml per 20 kg of the animal weight. Experienced (20 ani-mals) and control (10 animals) groups of lambs with an average live weight of 28 kg were selected from these lambs according to the principle of analogues. To the lambs of the control group, the drug was not given. 10 days after administration of the drug by clini-cal and coprological studies, it was found that the lambs of the experimental group, the intensity of nematode invasion decreased and the drug’s intensity (IE) was 96.7%, and the extensivity (EE) was 95%. The lambs of the experimental group before the introduction of the drug were 40% invaded with moniesia and 50% dictiocauli, and after using Monizen Forte they were released - the ex-tensibility (EE) was 100%. Lambs of the control (untreated) group, the intensity and extent of infection with nematodes and ces-todes remained at the same level as at the beginning of the experiment. All 10 lambs (50%) of the experimental group, the clinical signs of estrosis (sneezing, snorting, serous-mucous discharge from the nose) disap- peared, and the lambs of the control group have these clinical signs persisted.

scholarly journals Oral Administration of Lactobacillus Strains Isolated from Breast Milk as an Alternative for the Treatment of Infectious Mastitis during Lactation

2008 ◽  
Vol 74 (15) ◽  
pp. 4650-4655 ◽  
Author(s):  
E. Jiménez ◽  
L. Fernández ◽  
A. Maldonado ◽  
R. Martín ◽  
M. Olivares ◽  
...  
Keyword(s):  
Breast Milk ◽  
Clinical Signs ◽  
Control Group ◽  
Control Groups ◽  
Lactobacillus Salivarius ◽  
Probiotic Group ◽  
Efficient Alternative ◽  
The Mean ◽  
And Control ◽  
Infectious Mastitis

ABSTRACT In this study, 20 women with staphylococcal mastitis were randomly divided in two groups. Those in the probiotic group daily ingested 10 log10 CFU of Lactobacillus salivarius CECT5713 and the same quantity of Lactobacillus gasseri CECT5714 for 4 weeks, while those in the control one only ingested the excipient. Both lactobacillus strains were originally isolated from breast milk. On day 0, the mean staphylococcal counts in the probiotic and control groups were similar (4.74 and 4.81 log10 CFU/ml, respectively), but lactobacilli could not be detected. On day 30, the mean staphylococcal count in the probiotic group (2.96 log10 CFU/ml) was lower than that of the control group (4.79 log10 CFU/ml). L. salivarius CECT5713 and L. gasseri CECT5714 were isolated from the milk samples of 6 of the 10 women of the probiotic group. At day 14, no clinical signs of mastitis were observed in the women assigned to the probiotic group, but mastitis persisted throughout the study period in the control group women. In conclusion, L. salivarius CECT5713 and L. gasseri CECT5714 appear to be an efficient alternative for the treatment of lactational infectious mastitis during lactation.

scholarly journals Pathogenicity and Effects on Lymphoid Organs of Recent Moroccan Very Virulent Infectious Bursal Disease Virus in Broilers and SPF Chickens

2021 ◽  
Author(s):  
Charifa DRISSI TOUZANI ◽  
Imane MAAROUFI ◽  
Siham FELLAHI ◽  
Ikhlass EL BERBRI ◽  
Fatima-zohra SIKHT ◽  
...  
Keyword(s):  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Clinical Signs ◽  
Total Mortality ◽  
Bursa Of Fabricius ◽  
Infectious Bursal Disease ◽  
Control Group ◽  
Bursal Disease ◽  
Specific Pathogen ◽  
And Control

Abstract The aim of the current study is to evaluate the pathogenicity of recent infectious bursal disease virus (IBDV) (1/chicken/Morocco/IB19/2017) genetically characterized as vvIBDV belonging to genogroup 3.Two chicken lines, broiler and specific-pathogen-free (SPF) chickens, were inoculated by occulonasal route with 0.2 ml of the 105EID50 /ml of viral solution of IB19 vvIBDV strain at 29 days of age. The experimental monitoring was carried out during 10 days post challenge (dpc). The clinical signs stared on day 2 pc with maximum severity observed between 3 and 6 dpc. The total mortality rate reached 10% in broilers (group G1) and 93% in SPF (G3). The macroscopic lesions in broilers G1 was a marked hypertrophy of the bursa of Fabricius (BF) with slight haemorrhage observed between 2 to 4 dpc, followed by very pronounced atrophy observed on the 5 dpc. The post-mortem examinations of dead SPF birds (G3) revealed on 3 dpc very haemorrhagic BF with black cherry appearance in 80 % of dead birds. The mean Bursa/Body Index (BBI) of challenged broilers (G1) showed a decrease of 46% on day 9 pc compared to broilers control group (G2) indicating bursal atrophy. The microscopic lesions found in the BF on 3 dpc consisted mainly of inflammation with severe lymphoid depletion of the follicles. The evaluation of recent vvIBDV outbreak is very important to understand its epidemiology and will contribute to the efficient prevention and control of IBD.

scholarly journals Comparison of serum lead level of opium dependent patients with a healthy control group

2020 ◽  
Vol 5 (1) ◽  
pp. e08-e08
Author(s):  
Gholam Reza Masoomi ◽  
Zeinab Rastegar Chupani ◽  
Hazhir Heidari Beigvand ◽  
Mahdi Rezai ◽  
Shahin Dokht Hasan Pour
Keyword(s):  
Abdominal Pain ◽  
Lead Level ◽  
Clinical Signs ◽  
Control Group ◽  
Case Group ◽  
University Hospitals ◽  
Statistical Population ◽  
The Difference ◽  
Average Serum ◽  
And Control

Introduction: The prevalence of multiple symptoms, including mental illness, symptoms that mimic diseases such as pancreatitis, nonspecific abdominal pain and cholecystitis, are high in drug-dependent patients. Objectives: The aim of this study was to consider the clinical signs and serum lead levels of individuals referred to four university hospitals with various complaints. Patients and Methods: In this study, 128 sample cases in two groups, the case group and control group, have been examined. The case group included 64 persons using oral opium who were suffering from various complaints and referred to emergency department of mentioned hospitals. The control group included 64 patients without any history of addiction. Results: Mean of blood serum lead level in opium users and control group was 76.34±17.82 mg/dL and 7.68±3.72 mg/dL respectively, and the difference between these groups was significantly meaningful (P<0.001). The most prevalent complaints were abdominal pain and pulmonary complaints. Conclusion: Findings of the current study showed high levels for an average serum lead level for oral opium addicts among the studied statistical population. In addition, results show that ultimate diagnosis of lead poisoning is a factor justifying symptoms for addicts with nonspecific symptoms, the subject that confirms several previous reports. This finding asserts the necessity of screening of serum lead level for patients addicted to opium compounds in order to prevent more severe side effects.

Vaginal and intestinal microbiocenosis composition in pregnant women

2020 ◽  
Vol 68 (6) ◽  
pp. 29-36
Author(s):  
Alexandr A. Bezmenko ◽  
Natalya D. Sadovaya
Keyword(s):  
Pregnant Women ◽  
Normal Pregnancy ◽  
Main Group ◽  
Control Group ◽  
Microbiological Analysis ◽  
Control Groups ◽  
Threatened Miscarriage ◽  
Intestinal Dysbiosis ◽  
Grade Ii ◽  
And Control

Hypothesis/aims of study. It is assumed that intestinal dysbiosis, as well as bacterial vaginosis, may be one of the risk factors, and in some cases, the direct cause of pregnancy complications. This study was aimed to assess the intestinal and vaginal microbiocenosis composition in women with threatened miscarriage and normal pregnancy. Study design, materials and methods. The study involved 68 women aged 18 to 35 years (mean age 27.6 0.7 years) in pregnancy from 6 to 18 weeks. The main group consisted of 38 women with threatened miscarriage. The control group included women (n = 30) with normal pregnancy. Patients of the main and control groups were examined in accordance with the requirements of the Ministry of Health order No. 572n. In addition, a qualitative and quantitative microbiological analysis of vaginal discharge and feces was performed using the real-time polymerase chain reaction method. Results. Vaginal dysbiosis was detected in 60.5% of pregnant women of the main group and only 13.3% of women of the control group. In the main group, grade II dysbiosis was found in 23.7% of cases, and grade I dysbiosis in 36.8% of cases, while in the control group, these grades were found only in 3.3% and 10% of cases, respectively. Intestinal dysbiosis was diagnosed in 100% of the examined pregnant women. In patients of the main group, intestinal dysbiosis was detected as follows: grade I in 29% of cases, grade II in 52.6% of cases, and grade III in 18.4% of cases. In patients of the control group, the disorders corresponded mainly to grade I (83.4%) and, to a lesser extent, grade II (16.6%) dysbiosis, there being no cases of severe dysbiosis revealed. Conclusion. Women with threatened miscarriage had the most pronounced dysbiotic changes in vaginal and intestinal microflora compared to the control group. Vaginal dysbiosis was combined with intestinal dysbiosis in 100% of cases in patients of the main and control groups, and the degree of dysbiotic changes in the vaginal microflora was directly proportional to the degree of intestinal dysbiosis.

scholarly journals The use of scaning electron microscopy in postvaccinal evaluation of tracheal epithelium of Coturnix coturnix japonica

2003 ◽  
Vol 33 (1) ◽  
pp. 121-124 ◽  
Author(s):  
Elizabeth Santin ◽  
Fabiana S. Lima ◽  
Antônio C. Paulillo ◽  
Laura S. O. Nakaghi ◽  
Alex Maiorka
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Clinical Signs ◽  
Coturnix Japonica ◽  
Tracheal Epithelium ◽  
Control Group ◽  
Coturnix Coturnix Japonica ◽  
Coturnix Coturnix ◽  
Experimental Group ◽  
Scanning Electron

This study aimed at evaluating the use of scanning electron microscopy in the study of the post-vaccinal respiratory reaction of the tracheal epithelium of quails (Coturnix coturnix japonica) immunized against Newcastle disease. A number of 36 quails were distributed into four groups: T1 <FONT FACE=Symbol>¾</FONT> control birds (non-vaccinated); T2 <FONT FACE=Symbol>¾</FONT> birds vaccinated with Ulster 2C strain; t3 <FONT FACE=Symbol>¾</FONT> birds vaccinated with B1 strain; t4 <FONT FACE=Symbol>¾</FONT> birds vaccinated with LaSota strain. Regardless the experimental group, birds did not show detectable clinical signs of post-vaccinal respiratory reaction. However, the analysis of tracheal fragments by scanning electron microscopy showed that birds vaccinated with B1 and LaSota strains developed epithelial sloughing of the trachea, whereas those vaccinated Ulster 2C strain did not develop this change, demonstrating intact tracheal epithelium, similar to the control group.

A Pilot Study of Selective Lipopolysaccharide Adsorption and Coupled Plasma Filtration and Adsorption in Adult Patients with Severe Sepsis

2015 ◽  
Vol 39 (1-3) ◽  
pp. 210-217 ◽  
Author(s):  
Michail Yaroustovsky ◽  
Marina Abramyan ◽  
Nickolay Krotenko ◽  
Dmitry Popov ◽  
Marina Plyushch ◽  
...  
Keyword(s):  
Severe Sepsis ◽  
Standard Therapy ◽  
Clinical Signs ◽  
Oxygenation Index ◽  
Control Group ◽  
Combined Use ◽  
Plasma Filtration ◽  
Extracorporeal Therapy ◽  
The Mean ◽  
And Control

Aim: To evaluate the safety and effectiveness of combined extracorporeal therapy in patients with severe sepsis after cardiac surgery. Materials and Methods: Twenty patients received combined extracorporeal therapy (LPS-adsorption with Toraymyxin columns + CPFA). The inclusion criteria were clinical signs of severe sepsis, EAA = 0.6, and PCT >2 ng/ml. 20 comparable patients in the control group received only standard therapy. Results: Each patient in the study group received 2 daily treatments of combined extracorporeal therapy. In contrast to controls, we noted an increase in the values of MAP from 73 to 82 mm Hg, (p < 0.001) and the mean oxygenation index (from 180 to 246, p < 0.001), decrease of EAA from 0.77 to 0.55, p < 0.001, and PCT (from 6.23 to 2.83 ng/ml, p < 0.001). The 28-day survival rate was 65 and 35% in the study and control groups respectively, p = 0.11. Conclusion: The combined use of LPS-adsorption and CPFA in a single circuit with standard therapy is a safe and possibly effective adjunctive method for treating severe sepsis.

scholarly journals Effect of deslorelin on the induction of estrus in anestrous bitches

2010 ◽  
Vol 62 (3) ◽  
pp. 615-621 ◽  
Author(s):  
L.L. Lanna ◽  
A.P. Marques Jr. ◽  
R.H. Douglas
Keyword(s):  
Clinical Signs ◽  
Control Group ◽  
Progesterone Concentration ◽  
Plasma Progesterone ◽  
Clinical Evaluations ◽  
Injectable Formulation ◽  
Vaginal Cytology ◽  
Group 2 ◽  
And Control ◽  
Group 1

The efficacy of one or multiple doses of an injectable formulation of deslorelin (a GnRH agonist) was evaluated to induce estrus in anestrous bitches. Thirteen animals composed three groups: group 1 (n=5, single IM injection of 2mg deslorelin), group 2 (n=5, four IM injections of 2mg deslorelin in alternate days), and control group (n=3, four IM saline injections in alternate days). Daily clinical evaluations, sexual behavior, vaginal cytology, plasma progesterone concentration, ovaryhysterectomy and macroscopic evaluation of the uterus and ovaries were done. In group 1, none of the bitches showed signs of estrus, while two developed clinical signs and vaginal cytology of proestrus. In group 2, all animals presented proestrus, four presented estrus, and three ovulated; resulting in a functional corpus luteum and high progesterone concentration until day 25 of diestrus, when ovaryhysterectomy was performed. The duration of the stages of deslorelin induced cycles and the progesterone profile were similar to those described in the literature, and no side effects were observed. In conclusion, injectable formulation of deslorelin in multiple injections was effective to induce fertile estrus in anestrous bitches.

Sign in / Sign up

Export Citation Format

Share Document