Fraxin prevents knee osteoarthritis through inhibiting chondrocyte apoptosis in an experimental rat osteoarthritis model

2021 ◽  
Vol 28 ◽  
Author(s):  
Zhigang Qian ◽  
Xuanliang Ru ◽  
Chun Liu ◽  
Xiaoqin Huang ◽  
Qicai Sun
Keyword(s):  
Knee Osteoarthritis ◽  
Histological Analysis ◽  
Cartilage Damage ◽  
Chondrocyte Apoptosis ◽  
Control Group ◽  
Potential Candidate ◽  
New Approach ◽  
Knee Oa ◽  
Protein Levels ◽  
Blue Stain

Background: Current treatments of osteoarthritis are unsatisfied, a new approach towards the treatment of osteoarthritis is urged considering the state at present. Objectives: The objective of this study is to investigate the effect of fraxin on knee OA in a rat model and probe into the possible molecular mechanism. Methods: Primary Murine Chondrocytes were isolated and cell apoptosis analyses were performed. Rat OA models were established using meniscectomy method and allocated into three groups. Knee joint specimens were collected for qRT-PCR, western blotting and histological analysis. Statistical analyses were processed by using a SPSS. Results: The apoptosis rate of fraxin group is significantly reduced compared with the OA group or the control group. Fraxin remarkably down-regulated the expression of cleaved-Caspase-3 while significantly up-regulated the expression of Bcl-2, both on mRNA and protein levels. Toluidine blue stain results show relatively lighter articular cartilage damage compared with OA group. Conclusion: Fraxin prevents knee osteoarthritis by inhibiting chondrocyte apoptosis, which makes it a potential candidate as an anti-OA drug for clinical use.

scholarly journals A Positive Association Between Foot Posture Index and Medial Compartment Knee Osteoarthritis in Moroccan People

2014 ◽  
Vol 8 (1) ◽  
pp. 96-99 ◽  
Author(s):  
Abourazzak F.E ◽  
Kadi N ◽  
Azzouzi H ◽  
Lazrak F ◽  
Najdi A ◽  
...  
Keyword(s):  
Knee Osteoarthritis ◽  
Medial Compartment ◽  
Control Group ◽  
Flat Foot ◽  
Pes Cavus ◽  
Foot Posture Index ◽  
Posture Index ◽  
Knee Oa ◽  
Significant Difference ◽  
Foot Posture

Objectives : To compare foot posture in people with and without medial compartment knee osteoarthritis (OA), and to assess association between its abnormalities and medial compartment knee OA. Methods : We compared the foot posture of patients with clinically and radiographically-confirmed medial compartment knee OA and asymptomatic healthy controls using the foot posture index (FPI), navicular height, and the medial arch. Results : We included 100 patients and 80 asymptomatic controls. The mean age of patients was 59 ± 7 (44-76) years and 48 ± 9 (28-60) years in the control (p=0.06). Patients group have more pronated foot for FPI (1.50 ± 2.68 vs 0.72 ± 2.63; p=0.05), more flat foot (42% vs 22%; p=0.03), and less pes cavus than the control group (58% vs 77%; p=0.004). However, there was no significant difference between the groups in the navicular height (3.90 ± 0.85 cm vs 4.00 ± 0.76 cm; p=0.41). In multivariate statistical analysis, after adjusting for age and body mass index, pronated foot in FPI (OR=1.22, 95%IC= [1.06-1.40], p=0.005), and pes cavus (OR=0.32, 95%IC= [0.11-0.93], p=0.03) had a significant correlation with the knee osteoarthritis. Conclusion : Pronated foot posture and flat foot are significantly associated with medial compartment knee osteoarthritis.

scholarly journals Pengaruh Latihan Penguatan Dengan Elastic Band Dalam Meningkatkan Kemampuan Pasien Osteoarthritis Knee Di Rumah Sakit Condong Catur Sleman

2021 ◽  
Vol 2 (2) ◽  
pp. 89-94
Author(s):  
Wahyuni Wahyuni ◽  
Ricky Fauzi Zakaria
Keyword(s):  
Treatment Group ◽  
Knee Osteoarthritis ◽  
Functional Ability ◽  
Cartilage Damage ◽  
Control Group ◽  
Pain Level ◽  
Elastic Band ◽  
Control Group Design ◽  
Functional Capabilities ◽  
Strengthening Exercises

Background: Osteoarthritis is a degenerative disease caused by joint cartilage damage. The main symptoms of osteoarthritis are pain and movement disorders that can affect the functional disorders. The prevalence of knee osteoarthritis in Indonesia is quite high, reaching 15.5% in men and 12.7% in women. It is estimated that 1 to 2 million older people in Indonesia suffer from disabilities due to osteoarthritis. Osteoarthritis treatment is intended to reduce pain, improve functional ability, and prevent deformity. Elastic band strengthening exercises are exercises that can be used to reduce pain, increase muscle strength and functional ability.Objective: This study aims at determining the effect of elastic band strengthening exercises in improving knee osteoarthritis functional capabilities.Method: The method used in this study was quasi-experimental with pre and post-test control group design. The number of respondents of this study was 20 respondents diagnosed with knee osteoarthritis in Condong Catur Hospital Sleman. Respondents were randomly divided into two groups, namely the treatment group that received elastic band strengthening exercise treatment and the control group that received standard treatment given at the hospital. The treatment was given twice a week for four weeks. Measurement of functional ability was done using WOMAC score.Results: There was a decrease in pain level in the treatment group, while in the control group, the pain level was still the same as before. In the functional ability, there was an increase in the treatment group. The statistical test showed that there was an influence between the treatment group and the control group.Conclusion: Elastic band strengthening exercises effectively improved functional capabilities in knee osteoarthritis in Condong Catur Hospital Sleman.Keywords: elastic band strengthening exercises, functional capabilities, knee osteoarthritis

KNEE FLEXION EXTENSION AND STRENGTHENING (FELS) EXERCISE TO PAIN INTENSITY IN KNEE OSTEOARTHRITIS PATIENTS

2018 ◽  
Vol 2 (2) ◽  
pp. 274
Author(s):  
Marthalena Simamora ◽  
Galvani Volta Simanjuntak ◽  
Henny Syapitri
Keyword(s):  
Knee Osteoarthritis ◽  
Pain Intensity ◽  
Intervention Group ◽  
Control Group ◽  
Knee Oa ◽  
Randomized Design ◽  
Strengthening Exercise ◽  
Flexion Extension ◽  
Before And After ◽  
Quasi Experimental

Introduction. Osteoarthritis (OA) is a degenerative disease due to the thinning of the joint cartilage, mostly in the knee joint (89.91%) with the pain manifestation. If the pain is not managed, it could lead to the limitation of movement and disruption of daily activities. Having physical exercise regularly could reduce pain. The purpose of study was to determine the effect of knee fl exion extension and strengthening (FELS) exercise in reducing pain intensity. Methods. This was a quasi-experimental study with pretest-posttest control randomized design, where 70 respondents were recruited and consisted of 53 in intervention and 17 in control group. Result. The statistical analysis used ordinal regression revealed (1) FELS exercise was effective in reducing pain intensity (p = 0.013); (2) a signifi cant difference in pain intensity before and after FELS exercise (p = 0.000); (3) a signifi cant difference of pain intensity in the intervention group compared to the control group (p = 0.004); (4) effect of other activities in reducing pain intensity (p = 0.042). While the factors of age, gender, pain experience, anxiety and support system were not signifi cantly effective in reducing pain. Discussion. FELS exercise was helpful in reducing pain intensity in patients with knee osteoarthritis. It was then suggested to OA patients are requested to perform regular FELS exercise while the families are keep on giving support to the patient to do FELS exercise.Keywords: Knee fl exion extension, strengthening exercise, pain intensity, knee OA

Changes in postural control strategy during quiet standing in individuals with knee osteoarthritis

2021 ◽  
pp. 1-8
Author(s):  
Kento Sabashi ◽  
Satoshi Kasahara ◽  
Harukazu Tohyama ◽  
Takeshi Chiba ◽  
Yuta Koshino ◽  
...  
Keyword(s):  
Standard Deviation ◽  
Angular Velocity ◽  
Postural Control ◽  
Knee Osteoarthritis ◽  
Lower Limb ◽  
Center Of Mass ◽  
Three Dimensional ◽  
Quiet Standing ◽  
Control Group ◽  
Knee Oa

BACKGROUND: Knee osteoarthritis (OA) impairs postural control and may be affected by how the lower limb joints are used. OBJECTIVE: To investigate how individuals with knee OA use lower limb joints for static postural control. METHODS: Ten patients with knee OA and thirteen healthy controls performed quiet standing for 30 s. The standard deviation of the center of mass (COM) and lower limb joint motions in the anterior-posterior (AP) and medial-lateral (ML) planes were calculated from three-dimensional marker trajectories. Pearson’s correlation analysis and independent t-tests were conducted to investigate the relationship between COM and lower limb joint motion and to compare group difference, respectively. RESULTS: The AP hip angular velocity alone in the knee OA group and the AP hip and knee angular velocity in the control group were significantly correlated with the AP COM velocity. The ML hip angular velocity was significantly correlated with the ML COM velocity in both groups. The knee OA group exhibited a significantly larger standard deviation of AP COM velocity than the control group. CONCLUSIONS: Individuals with knee OA depended solely on the contribution of the hip to the AP COM velocity, which could not be successfully controlled by the knee.

The articular cartilage preservative effects of genistein in an experimental model of knees osteoarthritis

2021 ◽  
Author(s):  
Xiangjun Cheng ◽  
Peilian Xu
Keyword(s):  
Articular Cartilage ◽  
Knee Osteoarthritis ◽  
Experimental Model ◽  
Femoral Condyle ◽  
Cartilage Damage ◽  
Control Group ◽  
Articular Cartilage Damage ◽  
Genistein Treatment ◽  
Male Wistar Rats ◽  
Monosodium Iodoacetate

The study aimed to investigate the preservative effects of genistein on articular cartilage in an experimental model of knee osteoarthritis in rats. Thirty male Wistar rats were assigned to three equal groups: the sham group (SG), osteoarthritis control group (OAG), and genistein-treated osteoarthritis group (GTG). Intra-articular injections of monosodium iodoacetate were used for osteoarthritis induction. After two weeks of rest for the induction of the inflammatory process, genistein (30 mg/kg/day) vs. saline gavage was administered for eight weeks. The expression of matrix metalloproteinase (MMP) 8 and 13, Sox5/Sox6, Indian hedgehog (IHH), and Col2 were evaluated in medial femoral condyle sections by immunohistochemical staining. The number of chondrocytes and cartilage thicknesses were also measured and compared among the groups. No significant change in cartilage thickness was observed in GTG compared with OAG (p=0.188). Chondrocyte count was significantly higher in the articular cartilage of GTG compared with OAG (p=0.006). Induction of OA significantly increased the expression of MMP-8, MMP-13, and IHH, but decreased Col2, Sox5, and Sox6 expression (p<0.001); these were partially prevented in the GTG. Our findings support the effectiveness of genistein treatment in the prevention of articular cartilage damage in the experimental model of knee osteoarthritis. The proposed mechanism of action is through the suppression of the MMP, IHH, Col2 pathways, besides the induction of Sox5 and Sox6 expression. Novelty: -Genistein prevent articular cartilage damage in the experimental model of knee osteoarthritis. -The osteoprotective effect is trough modulation of expression of MMP, Sox, IHH, and Col2 proteins.

scholarly journals Investigation of Clinical Effects of High- and Low-Resistance Training for Patients With Knee Osteoarthritis: A Randomized Controlled Trial

2008 ◽  
Vol 88 (4) ◽  
pp. 427-436 ◽  
Author(s):  
Mei-Hwa Jan ◽  
Jiu-Jeng Lin ◽  
Jiann-Jong Liau ◽  
Yeong-Fwu Lin ◽  
Da-Hon Lin
Keyword(s):  
Knee Osteoarthritis ◽  
Resistance Exercise ◽  
Strength Training ◽  
High Resistance ◽  
Elderly Subjects ◽  
Control Group ◽  
Clinical Effects ◽  
Low Resistance ◽  
Knee Oa ◽  
Significant Difference

Background and PurposeMuscle strength training is important for people with knee osteoarthritis (OA). High-resistance exercise has been demonstrated to be more beneficial than low-resistance exercise for young subjects. The purpose of this study was to compare the effects of high- and low-resistance strength training in elderly subjects with knee OA.Subjects and MethodsOne hundred two subjects were randomly assigned to groups that received 8 weeks of high-resistance exercise (HR group), 8 weeks of low-resistance exercise (LR group), or no exercise (control group). Pain, function, walking time, and muscle torque were examined before and after intervention.ResultsSignificant improvement for all measures was observed in both exercise groups. There was no significant difference in any measures between HR and LR groups. However, based on effect size between exercise and control groups, the HR group improved more than the LR group.Discussion and ConclusionBoth high- and low-resistance strength training significantly improved clinical effects in this study. The effects of high-resistance strength training appear to be larger than those of low-resistance strength training for people with mild to moderate knee OA, although the differences between the HR and LR groups were not statistically significant.

scholarly journals Evaluating the efficacy of Internet-Based Exercise programme Aimed at Treating knee Osteoarthritis (iBEAT-OA) in the community: a study protocol for a randomised controlled trial

BMJ Open ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. e030564
Author(s):  
Sameer Akram Gohir ◽  
Paul Greenhaff ◽  
Abhishek Abhishek ◽  
Ana M. Valdes
Keyword(s):  
Randomised Controlled Trial ◽  
Knee Osteoarthritis ◽  
Rating Scale ◽  
Controlled Trial ◽  
Maximum Voluntary Contraction ◽  
Intervention Group ◽  
Exercise Programme ◽  
Control Group ◽  
Knee Oa ◽  
Randomised Controlled

IntroductionKnee osteoarthritis (OA) is the most common joint disease worldwide. As of today, there are no disease-modifying drugs, but there is evidence that muscle strengthening exercises can substantially reduce pain and improve function in this disorder, and one very well tested physiotherapy protocol is the ‘Better Management of Patients with Osteoarthritis’ developed in Sweden. Given the high prevalence of knee OA, a potentially cost-effective, digitally delivered approach to treat knee OA should be trialled. This study aims to explore the benefits of iBEAT-OA (Internet-Based Exercise programme Aimed at Treating knee Osteoarthritis) in modulating pain, function and other health-related outcomes in individuals with knee OA.Methods and analysisA randomised controlled trial was designed to evaluate the efficacy of a web-based exercise programme in a population with knee OA compared with standard community care provided by general practitioners (GPs) in the UK. We anticipate recruiting participants into equal groups. The intervention group (n=67) will exercise for 20–30 min daily for six consecutive weeks, whereas the control group (n=67) will follow GP-recommended routine care. The participants will be assessed using a Numerical Rating Scale, the Western Ontario and McMaster Universities Osteoarthritis Index, the Arthritis Research UK Musculoskeletal Health Questionnaire, the Pittsburgh Sleep Quality Index, 30 s sit to stand test, timed up and go test, quantitative sensory testing, musculoskeletal ultrasound scan, muscle thickness assessment of the vastus lateralis, and quadriceps muscles force generation during an isokinetic maximum voluntary contraction (MVC). Samples of urine, blood, faeces and synovial fluid will be collected to establish biomarkers associated with changes in pain and sleep patterns in individuals affected with knee OA. Standard parametric regression methods will be used for statistical analysis.Ethics and disseminationEthical approval was obtained from the Research Ethics Committee (ref: 18/EM/0154) and the Health Research Authority (protocol no: 18021). The study was registered in June 2018. The results of the trial will be submitted for publication in a peer-reviewed journal.Trial registration numberNCT03545048

scholarly journals Lower extremity venous diseases in primary knee osteoarthritis

2017 ◽  
Vol 89 (5) ◽  
pp. 53-59 ◽  
Author(s):  
O M Lesnyak ◽  
E V Zubareva ◽  
M G Goncharova ◽  
D M Maksimov
Keyword(s):  
Lower Extremity ◽  
Knee Osteoarthritis ◽  
Chronic Venous Insufficiency ◽  
Venous Insufficiency ◽  
Group Versus ◽  
Duplex Scanning ◽  
Control Group ◽  
Old Patients ◽  
Knee Oa ◽  
Venous Diseases

Aim. To establish a possible association between knee osteoarthritis (OA) and lower extremity venous diseases ((LEVDs) on the basis of thorough clinical and instrumental studies. Subjects and methods. A case-control study recruiting 40-60-year-old women was conducted. A study group included 85 women with knee OA; a control group consisted of 50 women without this condition. The patients of both groups underwent assessment of complaints and goal-directed objective examination to identify joint diseases and chronic LEVDs, knee X-ray study, and duplex scanning of the lower extremity veins. Results. The patients with knee OA were more frequently diagnosed with lower extremity varicose vein disease (VVD) (43% vs 22%; p=0.015) and had signs of chronic venous insufficiency (28% vs 12%; p=0.03). Duplex scanning of the lower extremities showed that knee OA was characterized by generalized LEVD (bilateral valve lesions of the great and small saphenous veins and severe valvular incompetence in the veins) that was detected in 53% of the patients in this group versus 20% of the women in the control group (p = 0.0004). After adjustment for body mass index, the differences in the incidence of VVD between the groups remained clinically and statistically significant (odds ratio (OR), 2.7; 95% confidence interval, 1.1—6.7; p=0.036). Conclusion. The 40—60-year-old patients with knee OA more commonly develop symptoms of chronic venous insufficiency than their healthy peers. Although obesity is a risk factor for both diseases, there is an independent association between knee OA and lower extremity VVD.

scholarly journals Effectiveness of Combination Myofascial Release and Kinesio Taping Intervention on Range of Motion Improvement in Elderly with Knee Osteoarthritis

2020 ◽  
Vol 1 (1) ◽  
pp. 13-16
Author(s):  
Afif Zainuri Wafiq ◽  
Atika Yulianti
Keyword(s):  
Knee Osteoarthritis ◽  
Range Of Motion ◽  
Cartilage Damage ◽  
The Elderly ◽  
Rank Test ◽  
Control Group ◽  
Case Group ◽  
Myofascial Release ◽  
Kinesio Taping ◽  
Significant Difference

Introduction: Knee Osteoarthritis is a condition that frequently occurs in elderly which has a major impact on the elderly’s disability. As a degenerative disease, knee osteoarthritis occurs due to cartilage damage which is then accompanied by inflammation, pain, stiff joints and muscles thereby limiting the range of motion. This research aims to compare the effect of Kinesio taping and a combination of myofascial release and Kinesio taping intervention on ROM improvement in elderly with knee osteoarthritis.Methods: This study applies a quasi-experimental design with a non-equivalent group approach. Goniometer is applied as a measurement instrument. 25 people participated in this study who were recruited through purposive sampling. The sample was then divided into 2 groups which were given treatment 3 times a week in one month. The control group with Kinesio Taping treatment consisted of 14 samples and the case group with a combination treatment of myofacial release and Kinesio taping consisted of 11 samples. Furthermore, data analysis was performed with the Shapiro Wilk test, Wilcoxon rank test, and the Mann-Whitney test using SPSS version 25.Results: The comparison test results show that the significance value of p yields 0.6> 0.05, therefore H1 is rejected, and H0 is accepted. The interpretation of these results is that there is no significant difference between the effects of Kinesio taping and the combination of myofacial release and Kinesio taping on increasing ROM in the elderly with the risk of knee osteoarthritis.Conclusion: This study shows that there is no significant difference in the effect of the treatment of Kinesio taping and the combination of myofacial release and Kinesio taping in increasing ROM in the elderly with osteoarthritis.

scholarly journals Hypoxia-inducible Factor Expression Is Related to Apoptosis and Cartilage Degradation in Temporomandibular Joint Osteoarthritis

2022 ◽  
Author(s):  
Jun Zhang ◽  
Yu Hu ◽  
Zihan Wang ◽  
Xuelian Wu ◽  
Chun Yang ◽  
...  
Keyword(s):  
Temporomandibular Joint ◽  
Expression Patterns ◽  
Cartilage Damage ◽  
Hypoxia Inducible Factor ◽  
Cartilage Degeneration ◽  
Chondrocyte Apoptosis ◽  
Control Group ◽  
Condylar Cartilage ◽  
Hypoxic Conditions ◽  
Temporomandibular Joint Osteoarthritis

Abstract Background: It remains unclear whether hypoxic conditions affect apoptosis and contribute to degradation of cartilaginous tissues in osteoarthritis (OA) lesions. In this study, we hypothesized that hypoxic conditions induced the accumulation of hypoxia-inducible factor (HIF) and activated apoptosis to contribute to OA cartilage degeneration in vivo.Methods: Malocclusion stress was applied for 2 weeks, 4 weeks and 8 weeks to induce an OA-like lesion animal model (OD) in rats. Histological analysis was performed by H&E staining and safranin O/fast green staining. The expression levels of protein in condylar cartilage were examined by immunostaining to evaluate cartilage degeneration.Results: We found apparent histological phenotypes associated with degeneration in the occlusion disorder stress (OD) group. The OD group at 4 weeks and 8 weeks had obviously reduced expression of Acan and Col II in cartilage. In contrast, the OD groups had higher levels of Col X, ADAMTS5 and MMP13 in the condylar cartilage than the control group. Moreover, the OD group cartilage had prominent degenerative changes with reduced levels of HIF1α and increased levels of HIF2α and the apoptosis factor Caspase3 in condylar cartilage at 8 weeks.Occlusion disorder stress results in cartilage degeneration. HIF1α and HIF2α are involved in temporomandibular joint (TMJ) cartilage homeostasis by regulating chondrocyte apoptosis, which contributes to TMJ cartilage degeneration. Conclusion: Thus, abnormal hypoxic conditions inducing opposite expression patterns of HIF1α and HIF2α could be involved in the pathogenesis of condylar cartilage degeneration. HIF2α may provide a potential negative feedback mechanism for HIF1α during cartilage damage.

Sign in / Sign up

Export Citation Format

Share Document