CORAL Software: Analysis of Impacts of Pharmaceutical Agents Upon Metabolism via the Optimal Descriptors

he current COVID-19 pandemic now believed to be based on the mutation of the SARS-CoV virus (first reported in 2002) to SARS-CoV-2 emerging in 2019, is naturally causing extreme worry and concern around the world with sometimes mixed and incoherent messages on how to deal with it. There is a plethora of information from previous epidemics caused by other coronaviruses such as severe acute respiratory syndrome, SARS (2002) and Middle East respiratory syndrome MERS (2012) from which we can extrapolate guidance on how to deal with the current pandemic. In the current absence of specific pharmaceutical agents, we propose assessing the extended tools that we already possess in our biological armoury to combat, prevent and control the spread of this virus. Using a set of precise criteria to locate such possible contenders, we conducted literature searches to find compounds that met these criteria. We have now reduced this to a shortlist of three agents that may be the best candidates. We propose vitamin C, vitamin D and Curcumin fit our criteria well. These compounds are widely available to the general public. They are available online and over-the-counter as supplements. Otherwise healthy individuals are safely able to self-administer these agents as a prophylactic to protect themselves and to enhance their immune response. This would be especially desirable for the elderly and at risk groups. These agents can also be used as adjunct therapy, particularly for those who may have early symptoms. This preventative therapy could be implemented whilst awaiting specific pharmaceutical drugs to emerge as a treatment for COVID-19. Our suggested compounds are a highly cost-effective way to potentially reduce the mortality that is regretfully mounting as a result of COVID-19 infection. The biological mode of action and the dosing of these compounds are summarised.

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Formation of feeding groups according to management accounting data

Buhuchet v sel'skom hozjajstve (Accounting in Agriculture) ◽

10.33920/sel-11-2006-03 ◽

2020 ◽

pp. 31-36

Author(s):

Boris Lukyanov ◽

Pavel Lukyanov

Keyword(s):

Software Package ◽

Management Accounting ◽

Coral Software ◽

Cattle Farm ◽

Accounting Data ◽

Feeding Groups

The article discusses the solution to the problem of uniting animals on a cattle farm into groups to organize their rational feeding. The task is solved based on the use of management accounting data; the solution of the problem is automated with the help of the CORAL software package

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Doping in sports – conscious lack of interest or ethical Everest?

Swiss Sports & Exercise Medicine ◽

10.34045/ssem/2017/27 ◽

2017 ◽

Vol 65 (4) ◽

Keyword(s):

Decision Making ◽

General Population ◽

Medical Setting ◽

Doping In Sports ◽

Physician Relationship ◽

Pharmaceutical Agents ◽

Similar Development ◽

Level Performance ◽

Elite Sports ◽

Support Decision Making

Within a clinical sports medical setting the discussion about doping is insufficient. In elite-sports use of pharmaceutical agents is daily business in order to maintain the expected top-level performance. Unfortunately, a similar development could be observed in the general population of leisure athletes where medical supervision is absent. As a sports physician you are facing imminent ethical questions when standing in between. Therefore, we propose the application of a standardised risk score as a tool to promote doping-prevention and launch the debate within athlete-physician-relationship. In the longterm such kind of risk stratification systems may support decision-making with regard to «protective» exclusion of sporting competition.

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An Overview of Optimization of Spherical Crystallisation Process

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2013.6.4.2 ◽

2013 ◽

Vol 6 (4) ◽

pp. 2203-2209

Author(s):

N B Bhagat ◽

A V Yadav ◽

P R Mastud ◽

R A Khutale

Keyword(s):

Physical Phenomenon ◽

Chemical Properties ◽

Spherical Shape ◽

Particle Engineering ◽

Spherical Crystallization ◽

Stirring Rate ◽

Physico Chemical ◽

Rate Selection ◽

Pharmaceutical Agents ◽

Spherical Crystals

In this article we describe the optimizing parameters in the process of spherical crystallisation. Particle engineering of active pharmaceutical agents is an innovative area of research in pharmaceutical industry because of several advantages. Spherical crystallization is one of the particle engineering technique in which drug directly gets crystallized and agglomerated into spherical shape. The spherical crystals can be obtained by different methods like solvent change, Quasi-emulsion droplet, ammonia diffusion and neutralisation. The optimization of process of spherical crystallization is important for obtaining the ideal spherical crystal agglomerates. It includes stirring rate, selection of solvent, pH, temperature etc. which affects on the physico-chemical properties of crystals. These optimizing parameters play its specific role in formation of spherical crystals. Stirring rate affects the shape as well as size of the final agglomerates and solvent selection helps in the formation of maximum amount of agglomerates in the system. The factors like pH and temperature should be maintained in case of drugs which show polymorphism. Apart from this, several others physical phenomenon or parameters like interfacial tension and rate of crystallisation are also important for thorough optimization of process.

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A Time-Resolved Low-Angle Light Scattering Apparatus. Application to Phase Separation Problems in Polymer Systems

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc20010973 ◽

2001 ◽

Vol 66 (6) ◽

pp. 973-982 ◽

Cited By ~ 5

Author(s):

Čestmír Koňák ◽

Jaroslav Holoubek ◽

Petr Štěpánek

Keyword(s):

Phase Separation ◽

Light Scattering ◽

Signal To Noise Ratio ◽

Scattered Light ◽

Ccd Camera ◽

Time Resolved ◽

Polymer Systems ◽

Software Analysis ◽

Phase Separation Kinetics ◽

Small Angle Light Scattering

A time-resolved small-angle light scattering apparatus equipped with azimuthal integration by means of a conical lens or software analysis of scattering patterns detected with a CCD camera was developed. Averaging allows a significant reduction of the signal-to-noise ratio of scattered light and makes this technique suitable for investigation of phase separation kinetics. Examples of applications to time evolution of phase separation in concentrated statistical copolymer solutions and dissolution of phase-separated domains in polymer blends are given.

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423. SARS-CoV-2 NGS Assay Powered by Biotia COVID-DX Software

Open Forum Infectious Diseases ◽

10.1093/ofid/ofaa439.617 ◽

2020 ◽

Vol 7 (Supplement_1) ◽

pp. S278-S279

Author(s):

Dorottya Nagy-Szakal ◽

Mara Couto-Rodriguez ◽

Joseph Barrows ◽

Heather L Wells ◽

Marilyne Debieu ◽

...

Keyword(s):

Genetic Variants ◽

Viral Genome ◽

Board Member ◽

Limit Of Detection ◽

Clinical Samples ◽

Analysis Tool ◽

Viral Titer ◽

Target Enrichment ◽

Library Preparation ◽

Software Analysis

Abstract Background COVID-19 had spread quickly, causing an international public health emergency with an alarming global shortage of COVID-19 diagnostic tests. We developed and clinically validated a next-generation sequencing (NGS)-based target enrichment assay with the COVID-DX Software tailored for the detection, characterization, and surveillance of the SARS-CoV-2 viral genome. Methods The SARS-CoV-2 NGS assay consists of components including library preparation, target enrichment, sequencing, and a COVID-DX Software analysis tool. The NGS library preparation starts with extracted RNA from nasopharyngeal (NP) swabs followed by cDNA synthesis and conversion to Illumina TruSeq-compatible libraries using the Twist Library Preparation Kit via Enzymatic Fragmentation and Unique Dual Indices (UDI). The library is then enriched for SARS-CoV-2 sequences using a panel of dsDNA biotin-labeled probes, specifically designed to target the SARS-CoV-2 genome, then sequenced on an Illumina NextSeq 550 platform. The COVID-DX Software analyzes sequence results and provides a clinically oriented report, including the presence/absence of SARS-CoV-2 for diagnostic use. An additional research use only report describes the assay performance, estimated viral titer, coverage across the viral genome, genetic variants, and phylogenetic analysis. Results The SARS-CoV-2 NGS Assay was validated on 30 positive and 30 negative clinical samples. To measure the sensitivity and specificity of the assay, the positive and negative percent agreement (PPA, NPA) was defined in comparison to an orthogonal EUA RT-PCR assay (PPA [95% CI]: 96.77% [90.56%-100%] and NPA [95% CI]: 100% [100%-100%]). Data reported using our assay defined the limit of detection to be 40 copies/ml using heat-inactivated SARS-CoV-2 viral genome in clinical matrices. In-silico analysis provided >99.9% coverage across the SARS-CoV-2 viral genome and no cross-reactivity with evolutionarily similar respiratory pathogens. Conclusion The SARS-CoV-2 NGS Assay powered by the COVID-DX Software can be used to detect the SARS-CoV-2 virus and provide additional insight into viral titer and genetic variants to track transmission, stratify risk, predict outcome and therapeutic response, and control the spread of infectious disease. Disclosures Dorottya Nagy-Szakal, MD PhD, Biotia (Employee) Mara Couto-Rodriguez, MS, Biotia (Employee) Joseph Barrows, MS, Biotia, Inc. (Employee, Shareholder) Heather L. Wells, MPH, Biotia (Consultant) Marilyne Debieu, PhD, Biotia (Employee) Courteny Hager, BS, Biotia (Employee) Kristin Butcher, MS, Twist Bioscience (Employee) Siyuan Chen, PhD, Twist Bioscience (Employee) Christopher Mason, PhD, Biotia (Board Member, Employee, Shareholder) Niamh B. O’Hara, PhD, Biotia (Board Member, Employee, Shareholder)Twist (Other Financial or Material Support, I am CEO of Biotia and Biotia has business partnership with Twist)

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Improvement of obesity-associated disorders by a small-molecule drug targeting mitochondria of adipose tissue macrophages

Nature Communications ◽

10.1038/s41467-020-20315-9 ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Yawei Wang ◽

Binlin Tang ◽

Lei Long ◽

Peng Luo ◽

Wei Xiang ◽

...

Keyword(s):

Adipose Tissue ◽

Oxidative Phosphorylation ◽

Near Infrared ◽

Mitochondrial Metabolism ◽

Diet Induced Obesity ◽

Adipose Tissue Macrophages ◽

Inflammatory Activation ◽

Treatment Of Obesity ◽

Pharmaceutical Agents

AbstractPro-inflammatory activation of adipose tissue macrophages (ATMs) is causally linked to obesity and obesity-associated disorders. A number of studies have demonstrated the crucial role of mitochondrial metabolism in macrophage activation. However, there is a lack of pharmaceutical agents to target the mitochondrial metabolism of ATMs for the treatment of obesity-related diseases. Here, we characterize a near-infrared fluorophore (IR-61) that preferentially accumulates in the mitochondria of ATMs and has a therapeutic effect on diet-induced obesity as well as obesity-associated insulin resistance and fatty liver. IR-61 inhibits the classical activation of ATMs by increasing mitochondrial complex levels and oxidative phosphorylation via the ROS/Akt/Acly pathway. Taken together, our findings indicate that specific enhancement of ATMs oxidative phosphorylation improves chronic inflammation and obesity-related disorders. IR-61 might be an anti-inflammatory agent useful for the treatment of obesity-related diseases by targeting the mitochondria of ATMs.

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