Detection of Metallothionein Proteins by Enzyme-Linked Immunosorbent Assay (ELISA)

2020 ◽  
Vol 21 (7) ◽  
pp. 544-554
Author(s):  
Qingyun Jia ◽  
Hans-Uwe Dahms ◽  
Lan Wang
Keyword(s):  
Structural Changes ◽  
Cross Validation ◽  
Radical Scavenging ◽  
Heavy Metal Stress ◽  
Clinical Samples ◽  
Enzyme Linked Immunosorbent Assay ◽  
Low Molecular Weight ◽  
Advantages And Disadvantages ◽  
Different Types ◽  
Redox Equilibria

Metallothioneins (MTs) are low-molecular-weight, cysteine-rich proteins that bind to heavy metals. MTs play a key role in the homeostasis of metal ions, maintaining intracellular redox equilibria and free radical scavenging. In several studies, under different conditions such as cancer development, drug therapy and heavy metal stress, the unique structural changes and functional effects of MT were studied. Although several assays are available to monitor the content and type of Metallothionein (MT) from environmental samples or in biomedical assays, Enzyme-Linked Immunosorbent Assays (ELISA) became the preferred method of MT detection. ELISA is low in cost, specific, simple, and efficient. This review evaluates the advantages and disadvantages of using different types of ELISA in the detection of metallothioneins from environmental or clinical samples as well as ways of its validation and cross-validation.

Studies on Chemical Methods to Expose Gate/Tunnel Oxide and Identification of Gate/Tunnel Oxide Defects in Wafer Fabrication

2002 ◽  
Author(s):  
Y. N. Hua ◽  
G. B. Ang ◽  
S. Redkar ◽  
Yogaspari ◽  
Wilma Richter
Keyword(s):  
Acetic Acid ◽  
Silicon Oxide ◽  
Wafer Fabrication ◽  
Chemical Methods ◽  
Advantages And Disadvantages ◽  
Different Types ◽  
Capacitor Structure ◽  
Oxide Defects ◽  
Buffer Oxide Etchant ◽  
Etch Time

Abstract In failure analysis of wafer fabrication, currently, three different types of chemical methods including 6:6:1 (Acetic Acid/HNO3/HF), NaOH and Choline are used in removing polysilicon (poly) layer and exposing the gate/tunnel oxide underneath. However, usage is limited due to their disadvantages. For example, 6:6:1 is a relatively fast etchant, but it is difficult to control the etch time and keep the oxide layer intact. Also, while using NaOH to remove poly and expose the silicon oxide, the solution needs to be heated. It is also difficult to etch a poly layer with a WSix or a CoSix silicide using NaOH. In this paper, we will discuss these 3 etchants in terms of their advantages and disadvantages. We will then introduce a new poly etchant, called HB91. HB91 is useful for removing poly to expose the gate/tunnel oxide for identification of related defects. HB91 is actually a mixture of two chemicals namely nitric acid (HNO3) and buffer oxide etchant (BOE) in a 9:1 ratio. The experimental results show that it is highly selective in poly removal with respect to the gate/tunnel oxide and is a suitable poly etchant especially for removing polysilicon with/without WSix and CoSix in the large capacitor structure. Application results of this poly etchant (HB91) will be presented.

Studies on the Behavior Some Paints in Electro-insulating Fluid Based on Vegetable Esters

2018 ◽  
Vol 69 (5) ◽  
pp. 1139-1144
Author(s):  
Iosif Lingvay ◽  
Adriana Mariana Bors ◽  
Livia Carmen Ungureanu ◽  
Valerica Stanoi ◽  
Traian Rus
Keyword(s):  
Structural Changes ◽  
Oxidation Processes ◽  
High Oleic ◽  
Painting Materials ◽  
Insulating Paper ◽  
Different Types ◽  
Mechanism And Kinetics ◽  
Kinetics Of ◽  
Natural Ester

For the purpose of using three different types of painting materials for the inner protection of the transformer vats, their behavior was studied under actual conditions of operation in the transformer (thermal stress in electro-insulating fluid based on the natural ester in contact with copper for electro-technical use and electro-insulating paper). By comparing determination of the content in furans products (HPLC technique) and gases formed (by gas-chromatography) in the electro-insulating fluid (natural ester with high oleic content) thermally aged at 130 �C to 1000 hours in closed glass vessels, it have been found that the presence the investigated painting materials lead to a change in the mechanism and kinetics of the thermo-oxidation processes. These changes are supported by oxygen dissolved in oil, what leads to decrease both to gases formation CO2, CO, H2, CH4, C2H4 and C2H6) and furans products (5-HMF, 2-FOL, 2 -FAL and 2-ACF). The painting materials investigated during the heat treatment applied did not suffer any remarkable structural changes affecting their functionality in the electro-insulating fluid based on vegetable esters.

Anticancer Properties of Essential Oils: An Overview

2018 ◽  
Vol 18 (10) ◽  
pp. 957-966 ◽  
Author(s):  
Milene Aparecida Andrade ◽  
Mariana Aparecida Braga ◽  
Pedro Henrique Souza Cesar ◽  
Marcus Vinicius Cardoso Trento ◽  
Mariana Araújo Espósito ◽  
...  
Keyword(s):  
Cancer Therapy ◽  
Essential Oils ◽  
Public Health Problem ◽  
Mechanisms Of Action ◽  
Low Molecular Weight ◽  
Anticancer Properties ◽  
Anti Cancer ◽  
Different Types ◽  
Antitumor Properties ◽  
Pharmaceutical Properties

Background: Essential oils are complex mixtures of low molecular weight compounds extracted from plants. Their main constituents are terpenes and phenylpropanoids, which are responsible for their biological and pharmaceutical properties, such as insecticidal, parasiticidal, antimicrobial, antioxidant, anti-inflammatory, analgesic, antinociceptive, anticarcinogenic, and antitumor properties. Cancer is a complex genetic disease considered as a serious public health problem worldwide, accounting for more than 8 million deaths annually. Objective: The activities of prevention and treatment of different types of cancer and the medicinal potential of essential oils are addressed in this review. Conclusion: Several studies have demonstrated anti-carcinogenic and antitumor activity for many essential oils obtained from various plant species. They may be used as a substitution to or in addition to conventional anti-cancer therapy. Although many studies report possible mechanisms of action for essential oils compounds, more studies are necessary in order to apply them safely and appropriately in cancer therapy.

Lessons Not Learned: Why is There Still a Crisis-Level Shortage of Accounting Ph.D.s?

2014 ◽  
Vol 28 (2) ◽  
pp. 313-330 ◽  
Author(s):  
R. David Plumlee ◽  
Philip M. J. Reckers
Keyword(s):  
Student Perceptions ◽  
Structural Changes ◽  
Ad Hoc ◽  
Doctoral Programs ◽  
Cumulative Impact ◽  
Program Administrators ◽  
Accounting Faculty ◽  
Present Information ◽  
Different Types

SYNOPSIS: In 2005, an ad hoc committee appointed by the American Accounting Association (AAA) documented a crisis-level shortage of accounting Ph.D.s and recommended significant structural changes to doctoral programs (Kachelmeier, Madeo, Plumlee, Pratt, and Krull 2005). However, subsequent studies show that the shortage continues and the cumulative costs grow (e.g., Fogarty and Holder 2012; Brink, Glasscock, and Wier 2012). The Association to Advance Collegiate Schools of Business (AACSB) recently called for renewed attention to the problem (AACSB 2013b). We contribute to the literature by providing updated information regarding responses by doctoral programs and, from the eyes of potential candidates, of continuing impediments to solving the doctoral shortage. In this paper, we present information gathered through surveys of program administrators and master's and Accounting Doctoral Scholars Program (ADS) students. We explore (1) the cumulative impact of the Ph.D. shortage as of 2013, including its impact on accounting faculty composition, across different types of institutions, (2) negative student perceptions of Ph.D. programs and academic accounting careers, which discourage applicants from pursuing Ph.D. programs, and (3) impediments facing institutions in expanding doctoral programs.

scholarly journals Going virtual: youth attitudes toward and experiences of virtual mental health and substance use services during the COVID-19 pandemic

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Lisa D. Hawke ◽  
Natasha Y. Sheikhan ◽  
Karen MacCon ◽  
Joanna Henderson
Keyword(s):  
Mental Health ◽  
Substance Use ◽  
Youth Mental Health ◽  
Research Process ◽  
Clinical Samples ◽  
Future Research ◽  
Advantages And Disadvantages ◽  
Substance Use Services ◽  
Youth Attitudes ◽  
Virtual Services

Abstract Background During the COVID-19 pandemic, youth mental health and substance use services rapidly moved to virtual modalities to meet social distancing requirements. It is important to understand youth attitudes toward and experience of virtual services. Objective This study examined the attitudes toward and experiences of virtual mental health and substance use services among youth drawn from clinical and non-clinical samples. Method Four hundred nine youth completed a survey including questions about their attitudes toward and experience of virtual services. The survey included quantitative and open-ended questions on virtual care, as well as a mental health and substance use screener. Results The majority of youth with mental health or substance use challenges would be willing to consider individual virtual services, but fewer would consider group virtual services. However, many have not received virtual services. Youth are interested in accessing a wide variety of virtual services and other supportive wellness services. Advantages and disadvantages of virtual services are discussed, including accessibility benefits and technological barriers. Discussion As youth mental health and substance use services have rapidly gone virtual during the COVID-19 pandemic, it is essential that we hear the perspectives of youth to promote service utilization among those in need. Diverse, accessible, technologically stable virtual services are required to meet the needs of different youth, possibly with in-person options for some youth. Future research, engaging youth in the research process, is needed to evaluate the efficacy of virtual services to plan for the sustainability of some virtual service gains beyond the pandemic period.

scholarly journals Inhibitory effect of low-molecular-weight peptides (0–3 kDa) from Spirulina platensis on H2O2-induced oxidative damage in L02 human liver cells

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Jun Ma ◽  
Xiankun Zeng ◽  
Min Zhou ◽  
Le Cheng ◽  
Difeng Ren
Keyword(s):  
Molecular Weight ◽  
Oxidative Damage ◽  
Spirulina Platensis ◽  
Radical Scavenging ◽  
Inhibitory Effect ◽  
Control Group ◽  
Low Molecular Weight ◽  
Sod Activity ◽  
Liver Health ◽  
L02 Cells

AbstractSpirulina platensis protein hydrolysates were prepared by digesting protein extracts with papain, and the hydrolysates were separated into 30, 10, and 3 kDa weights using membrane ultrafiltration. The 0–3 kDa low-molecular-weight Spirulina peptides (LMWSPs) proved the highest chemical antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging ability, hydroxyl radical (·OH) scavenging activities and total antioxidant capacity. Cellular antioxidant ability of LMWPs fractions against 2000 μg/mL H2O2 induced oxidative damage of L02 cells were investigated. The MTT assay results displayed that LMWSPs at different concentrations (0–1000 μg/mL) had proliferation effect on the L02 cells and that treatment of the L02 cells with the 1000 μg/mL LMWSPs (0–3 kDa) significantly prevented H2O2-induced oxidative damage compared with control cells. Moreover, the 2′,7′-dichlorofluorescein diacetate (DCFH-DA) fluorescent probe assay showed that the levels of ROS and NO were significantly lower in the experimental group that was treated with the peptides for 24 h than in the control group. Furthermore, using the corresponding kits, the treatment inhibited the reduction of SOD activity and the increase of MDA contents in the L02 cells. Therefore, LMWSPs (0–3 kDa) may have potential applications in antioxidant and liver health products.

scholarly journals Genetic Characterization of Methicillin Resistant and Sensitive, Vancomycin Intermediate Staphylococcus aureus Strains Isolated from Different Iranian Hospitals

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Seyed Asghar Havaei ◽  
Amir Azimian ◽  
Hosein Fazeli ◽  
Mahmood Naderi ◽  
Kiarash Ghazvini ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Genetic Analysis ◽  
Clinical Samples ◽  
Different Types ◽  
Health Care Policies ◽  
Global Health Care ◽  
Vancomycin Resistant ◽  
Epidemiological Evaluation ◽  
Mrsa Strains

Background. Global concerns have been raised due to upward trend of Vancomycin Intermediate Staphylococcus aureus (VISA) and Vancomycin Resistant Staphylococcus aureus (VRSA) reports which mean casting doubt on the absolute effectiveness of the last line of antibiotic treatment for S. aureus, vancomycin. Hence, epidemiological evaluation can improve global health care policies. Methodology. 171 Isolates of Staphylococcus aureus were collected from different types of clinical samples in selected hospitals in Isfahan, Mashhad, and Tehran, Iran. Then, they were evaluated by agar screening, disk diffusion, and MIC method to determine their resistance to vancomycin and methicillin. The isolated VISA strains were then confirmed with genetic analysis by the evaluation of mecA and vanA genes, SCCmec, agr, and spa type, and also toxin profiles. MLST was also performed. Results and Conclusion. Our data indicated that 67% of isolated S. aureus strains were resistant to methicillin. Furthermore, five isolates (2.9%) had intermediate resistance to vancomycin (VISA). In contrast to usual association of VISA with MRSA strains, we found two isolates of MSSA-VISA. Therefore, our data suggests a probable parallel growing trend of VISA towards MSSA, along with MRSA strains. However, more samples are required to confirm these primarily data. Moreover, genetic analysis of the isolated VISA strains revealed that these strains are endemic Asian clones.

MMPI-2 L + K and L + K — F Indexes of Underreporting: Normative and Desirable Responding

2003 ◽  
Vol 92 (1) ◽  
pp. 223-227 ◽  
Author(s):  
Alfred L. Brophy
Keyword(s):  
Minnesota Multiphasic Personality Inventory ◽  
Personality Inventory ◽  
Clinical Samples ◽  
Normative Sample ◽  
Random Responding ◽  
Different Types

Norms based on the Minnesota Multiphasic Personality Inventory-2 (MMPI-2) normative sample are provided for the L + K and L + K – F indexes of underreporting and defensiveness. Index scores produced by consistently desirable responding, which was guided by desirability eatings of the MMPI-2 items, also are provided and compared with scores produced by underreporting in other studies. The indexes correlate highly with other measures of underreporting in both normative and clinical samples. The L and K scales assess different types of underreporting and should be interpreted separately as well as in combination. The L scale can be elevated by undesirable responding or random responding in addition to desirable responding.

Enhanced thermal stability and lifetime of epoxy nanocomposites using covalently functionalized clay: experimental and modelling

2015 ◽  
Vol 39 (3) ◽  
pp. 2269-2278 ◽  
Author(s):  
Omid Zabihi ◽  
Hamid Khayyam ◽  
Bronwyn L. Fox ◽  
Minoo Naebe
Keyword(s):  
Thermal Stability ◽  
Thermal Degradation ◽  
Structural Changes ◽  
Epoxy Nanocomposites ◽  
Degradation Model ◽  
Different Types

Relationship between thermal degradation model of epoxy nanocomposites containing different types of modified nanoclay and their structural changes is explained.

Effects of Metal Stress on Visible/Near-Infrared Reflectance Spectra of Vegetation

2011 ◽  
Vol 347-353 ◽  
pp. 2735-2738 ◽  
Author(s):  
Guang Yu Chi ◽  
Yi Shi ◽  
Xin Chen ◽  
Jian Ma ◽  
Tai Hui Zheng
Keyword(s):  
Heavy Metal ◽  
Structural Changes ◽  
Near Infrared ◽  
Leaf Surface ◽  
Spectral Response ◽  
Spectral Characteristics ◽  
Surface Characteristics ◽  
Heavy Metal Stress ◽  
Metal Stress ◽  
Near Infrared Reflectance

Vegetation which suffers from heavy metal stresses can cause changes of leaf color, shape and structural changes. The spectral characteristics of vegetation leaves is related to leaf thickness, leaf surface characteristics, the content of water, chlorophyll and other pigments. So the eco-physiology changes of plants can be reflected by spectral reflectance. Studies on the spectral response of vegetation to heavy metal stress can provide a theoretical basis for remote sensing monitoring of metal pollution in soils. In recent decades, there are substantial amounts of literature exploring the effects of heavy metals on vegetation spectra.

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