scholarly journals A Comprehensive Investigation of Potential Novel Marine Psychrotolerant Actinomycetes sp. Isolated from the Bay-of-Bengal

2020 ◽  
Vol 21 (4) ◽  
pp. 271-282 ◽  
Author(s):  
Mrinmoy Ghosh ◽  
Meeta Gera ◽  
Joginder Singh ◽  
Ram Prasad ◽  
Krishna Kanth Pulicherla
Keyword(s):  
Bay Of Bengal ◽  
Expression Profiling ◽  
Growth Conditions ◽  
Antimicrobial Activities ◽  
The Novel ◽  
Bioactive Natural Products ◽  
Antibacterial And Antifungal Activities ◽  
Good Resource ◽  
Antibacterial And Antifungal ◽  
Differential Expression Profiling

Background: This study was carried out to classify the diversity of the deep marine psychrotolerant actinomycetes sp. nov., in the Bay of Bengal and exploit the production of coldactive industrial and pharmaceutical biomolecules. Objective: 1) Characterization, optimum the growth conditions and classify the diversity of the novel isolated deep marine psychrotolerant actinomycetes sp from the Bay-of-Bengal. 2) Screening for industrially important biocatalysts and determine the antimicrobial activities against the five dreadful pathogens. 3) The differential expression profiling of the candidate genes to regulate the biosynthesis of selected enzymes. Methods: The cold-adapted actinomycetes were isolated from the deep marine water collections at 1200 mts below the surface in Bay-of-Bengal. The phenotypic and genotypic characterizations have been carried out to understand the persistent diversity of this novel marine psychrotolerant actinomycetes species. The production of cold-active enzymes, such as amylase, cellulase, lipase, pectinase, and L-asparaginase, were screened and the expression profiling genes were determined by using qRT PCR. The antibacterial and antifungal activities have also been investigated. Results: A total number of 37 novel actinomycetes were isolated and the phenotypic and genotypic characterizations identified the genus, dominated by Streptomyces (17 distinct sub-groups) as the major group, followed by Micromonospora, Actinopolyspora, Actinosynnema, Streptoverticillium, Saccharopolyspora, Nocardiopsis, and Nocardia. The optimum growth and abundant mycelium formation are observed at 15°C to 20°C and also capability for thriving at 4°C. All the isolates exhibited a significant role in the production of biocatalysts, and the antagonistic activities were also noted against five major selected pathogens. Conclusion: The Streptomyces from the Bay-of-Bengal have high biosynthetic potential and can serve as a good resource for the exploration of bioactive natural products

Synthesis & Anticancer Evaluation of New Substituted 2-(3,4- Dimethoxyphenyl)benzazoles

2019 ◽  
Vol 15 (3) ◽  
pp. 287-297 ◽  
Author(s):  
Cigdem Karaaslan ◽  
Yalcin Duydu ◽  
Aylin Ustundag ◽  
Can O. Yalcin ◽  
Banu Kaskatepe ◽  
...  
Keyword(s):  
Hela Cells ◽  
Parp Inhibitor ◽  
A549 Cells ◽  
Human Tumor ◽  
Antimicrobial Activities ◽  
Sodium Metabisulfite ◽  
Human Tumor Cell Lines ◽  
Antibacterial And Antifungal Activities ◽  
Antibacterial And Antifungal ◽  
Mitotic Inhibitor

Background: The benzazole nucleus is found in many promising small molecules such as anticancer and antibacterial agents. Bendamustine (Alkylating agent), Nocodazole (Mitotic inhibitor), Veliparib (PARP inhibitor), and Glasdegib (SMO inhibitor) are being clinically used as anticancer therapeutic which bear benzimidazole moiety. Based on the principle of bioisosterism, in the present work, 23 compounds belonging to 2-(3,4-dimethoxyphenyl)benzazoles and imidazopyridine series were synthesized and evaluated for their anticancer and antimicrobial activities. Objective: A series of new 2-(3,4-dimethoxyphenyl)-1H-benz(or pyrido)azoles were synthesized and evaluated for their anticancer and antimicrobial activities. Method: N-(5-chloro-2-hdroxyphenyl)-3,4-dimethoxybenzamide 1, was obtained by the amidation of 2-hydroxy-5-chloroaniline with 3,4-dimethoxybenzoic acid by using 1,1&'-carbonyldiimidazole. Cyclization of 1 to benzoxazole derivative 2, was achieved by p-toluenesulfonic acid. Other 1H-benz(or pyrido)azoles were prepared by the reaction between 2-aminothiophenol, ophenylenediamine, o-pyridinediamine with sodium metabisulfite adduct of 3,4-dimethoxybenzaldehyde. The NMR assignments of the dimethoxy groups were established by the NOESY spectra. Results: Compound 12, bearing two chlorine atoms at the 5(4) and 7(6) positions of the benzene moiety of benzimidazole was found the most potent analogue against A549 cells with the GI50 value of 1.5 μg/mL. Moreover, 24 showed remarkable cell growth inhibition against MCF-7 and HeLa cells with the GI50 values of 7 and 5.5 μg/mL, respectively. The synthesized compounds have no important antibacterial and antifungal activities. Conclusion: It could be concluded that the introduction of di-chloro atoms at the phenyl ring of 2-(3,4-dimethoxyphenyl)-1H-benzimidazoles increases significant cytotoxicity to selected human tumor cell lines in comparison to other all benzazoles synthesized. Unsubstituted 2-(3,4- dimethoxyphenyl)-imidazopyridines also gave good inhibitory profile against A549 and HeLa cells.

scholarly journals Synthesis and antimicrobial activities of some (E)-N'-1-(substituted benzylidene)benzohydrazides

2017 ◽  
Vol 5 (1) ◽  
pp. 17 ◽  
Author(s):  
V. Manikandan ◽  
S. Balaji ◽  
R. Senbagam ◽  
R. Vijayakumar ◽  
M. Rajarajan ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Fourier Transform ◽  
Magnetic Resonance ◽  
Spectral Data ◽  
Ultra Violet ◽  
Antimicrobial Activities ◽  
Antibacterial And Antifungal Activities ◽  
Ft Ir ◽  
Fourier Transform Ir ◽  
Antibacterial And Antifungal

About ten substituted (E)-N'-1-(substituted benzylidene) benzohydrazides have been synthesized. They are analyzed by their analytical, ultra violet (UV), Fourier transform-IR (FT-IR) and nuclear magnetic resonance (NMR) spectral data and evaluated by antimicrobial activities such antibacterial and antifungal activities.

scholarly journals Gel Formulation of Ethyl Acetate Garlic Extraction and Its Activity Against Staphylococcus Epidermis

2019 ◽  
Vol 1 (2) ◽  
pp. 69-78
Author(s):  
Khairan Khairan
Keyword(s):  
Ethyl Acetate ◽  
Staphylococcus Epidermidis ◽  
Antimicrobial Activities ◽  
Phytochemical Screening ◽  
Viscosity Property ◽  
Antibacterial And Antifungal Activities ◽  
Staphylococcus Epidermis ◽  
Homogeneity Property ◽  
The Stability ◽  
Antibacterial And Antifungal

Garlic is known to have antibacterial and antifungal activities. This research aims to formulate the gel materials extracted from the garlic and to investigate the antimicrobial activities to Staphylococcus epidermidis. The phytochemical screening showed that the gel materials of ethyl acetate consisted alkaloid, flavonoid, and saponin substances. The formulation of gel was synthesized with different variations of 1, 5, and 10%. In this research, the gel materials were analyzed to understand the stability, homogeneity, pH, viscosity and dispersive powers.  The results showed that good abilities of gel based on the taste, aroma and consistency, while the homogeneity property of each samples is influenced by the presence of gel concentration. The viscosity property of gels accounted for 3201.02-1664.04 Cps with 2.95-3.8 cm of dispersive powers. The ability of antimicrobial showed that higher concentration of 5% and 10% gels showed greater inhibitory zones of 18,10 and 22,08 mm compared to 1% of concentration

scholarly journals Synthesis,Characterization and Evaluation of the Anticancer and Antimicrobial Activities of Some Novel Benzazole and Benzazine Derivatives

2007 ◽  
Vol 3 (3) ◽  
pp. 252-263
Author(s):  
Wesam Saber Shehab ◽  
Naglaa Z.H. Eleiwa ◽  
Samar.M. Mouneir
Keyword(s):  
Cancer Cells ◽  
Benzimidazole Derivative ◽  
Coupling Reaction ◽  
Antimicrobial Activities ◽  
Spectral Studies ◽  
Design Modification ◽  
Future Design ◽  
Antibacterial And Antifungal Activities ◽  
Antibacterial And Antifungal

The present study was designed to synthesize  and develop new useful lead compounds (some novel benzazole and benzazine derivatives ) of simple structure , exhibiting optimal in vitro anticancer  and antimicrobial potency. Phenylenediamine derivative 1 was condensed  with  dithiocarboxylic acid derivatives 2 and produced   benzimidazole derivative 4. The benzotriazepines 8 and 10  were formed by the reaction of 1 with dicarbonyl derivatives followed by intermolecular coupling reaction. The synthesis of benzotriazine12, benzotriazole 14,17, benzimidazole16 and benzothiadiazine 19 from compound 1 was also described. The Synthesized Compounds were characterized by Spectral Studies like IR, H1 – NMR and Analysis Spectra .The title compounds were screened for their possible In vitro anticancer and antimicrobial activities. Among the synthesized compounds, some have shown promisingly remarkable activities against  different  cancer cell lines (MCF-7 human breast cancer cells, HepG2 human hepatocarcinoma cells and PC3 human prostate cancer cells)and moderate  to high antibacterial and antifungal activities. The obtained results showed that the most active compounds could be useful as a template for future design, modification and investigation to produce more active analogs.

Synthesis and investigation of 3,5-bis-linear and macrocyclic tripeptidopyridine candidates by using l-valine, N,N′-(3,5-pyridinediyldicarbonyl)bis-dimethyl ester as synthon

2019 ◽  
Vol 74 (6) ◽  
pp. 473-478 ◽  
Author(s):  
Abd El-Galil E. Amr ◽  
Ahmed M. Naglah ◽  
Nermien M. Sabry ◽  
Alhussein A. Ibrahim ◽  
Elsayed A. Elsayed ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Organic Molecules ◽  
Biological Activities ◽  
Dimethyl Ester ◽  
Antimicrobial Activities ◽  
Current Work ◽  
Antifungal Activities ◽  
Antibacterial And Antifungal Activities ◽  
Antibacterial And Antifungal

AbstractInterest in the synthesis of heterocyclic organic molecules with peptide moieties has gained attention due to their potential biological activities. The current work aimed at synthesizing new macrocyclic tripeptide imides and evaluating their possible antimicrobial activities. A series of 11 derivatives were prepared from dimethyl 3,5-pyridinevalinyl ester either by NaOH or NH2NH2 treatment, followed by cyclization and further reaction with NaOH or NH2NH2. The majority of synthesized derivatives showed promising antibacterial and antifungal activities in comparison to standard known antibiotics. Compounds 5a and 7b showed the most potential antibacterial against Staphylococcus aureus and antifungal activities against Candida albicans, respectively.

scholarly journals In VitroPhytochemical, Antibacterial, and Antifungal Activities of Leaf, Stem, and Root Extracts ofAdiantum capillus veneris

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Muhammad Saqib Ishaq ◽  
Muhammad Medrar Hussain ◽  
Muhammad Siddique Afridi ◽  
Ghadir Ali ◽  
Mahrukh Khattak ◽  
...  
Keyword(s):  
Multidrug Resistant ◽  
Antimicrobial Activities ◽  
Phytochemical Analysis ◽  
Bacterial Strains ◽  
Antifungal Activities ◽  
Fungal Strains ◽  
Antibacterial And Antifungal Activities ◽  
Adiantum Capillus Veneris ◽  
Ethanol Extracts ◽  
Antibacterial And Antifungal

Adiantum capillus venerisis a medicinally essential plant used for the treatment of diverse infectious diseases. The study of phytochemical and antimicrobial activities of the plant extracts against multidrug-resistant (MDR) bacteria and medically important fungi is of immense significance. Extracts from the leaves, stems, and roots ofAdiantum capillus veneriswere extracted with water, methanol, ethanol, ethyl acetate, and hexane and screened for their antimicrobial activity against ten MDR bacterial strains and five fungal strains isolated from clinical and water samples. Ash, moisture, and extractive values were determined according to standard protocols. FTIR (Fourier transform infrared Spectroscopy) studies were performed on different phytochemicals isolated from the extracts ofAdiantum capillus Veneris. Phytochemical analysis showed the presence of flavonoids, alkaloids, tannins, saponins, cardiac glycosides, terpenoids, steroids, and reducing sugars. Water, methanol, and ethanol extracts of leaves, stems, and roots showed significant antibacterial and antifungal activities against most of the MDR bacterial and fungal strains. This study concluded that extracts ofAdiantum capillus venerishave valuable phytochemicals and significant activities against most of the MDR bacterial strains and medically important fungal strains.

scholarly journals Synthesis, Characterization and Study of Antimicrobial Activities of Mannich Bases Incorporating 1,2,4-Triazole Nucleus

2021 ◽  
Vol 2 (01) ◽  
pp. 19-27
Author(s):  
Rhambus Rawat ◽  
Prem Shankar Deo ◽  
Bhushan Shakya
Keyword(s):  
Schiff Base ◽  
Heterocyclic Compounds ◽  
Environmental Science ◽  
Biological Activities ◽  
Antimicrobial Activities ◽  
Mannich Bases ◽  
New Drugs ◽  
Spectroscopic Techniques ◽  
Antibacterial And Antifungal Activities ◽  
Antibacterial And Antifungal

Heterocyclic compounds containing triazole moiety have great importance in the field of medicine, pharmaceuticals, biochemistry, biology, therapeutics, environmental science, and industry. Triazoles and their derivatives have been extensively used in the development of new drugs. Biological activities of Schiff bases are highly investigated, but Mannich bases are on the verge of their development, and they are being synthesized in large number nowadays. In this work, Mannich bases are synthesized by incorporation 1,2,4-triazole moiety through Schiff base using different amines. Mannich bases are found to exhibit highly effective antibacterial and antifungal activities. The formation of synthesized compounds - 1,2,4-triazole-5-thione, Schiff base (4) and Mannich bases (5a and 5b) - are confirmed and characterized by spectroscopic techniques like UV, FTIR 1H-NMR and 13C-NMR. The activity of the synthesized compounds was tested against bacterial and fungal strain.

scholarly journals Antibacterial Potential of Citral Derivatives

2011 ◽  
Vol 6 (9) ◽  
pp. 1934578X1100600
Author(s):  
Soni A. Singh ◽  
Yogesh A. Potdar ◽  
Rasika S. Pawar ◽  
Sujata V. Bhat
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Activities ◽  
Serial Dilution ◽  
Disc Diffusion ◽  
Antifungal Activities ◽  
Antibacterial And Antifungal Activities ◽  
Antibacterial And Antifungal ◽  
Antibacterial Potential

Nine conjugated acid derivatives from monoterpene citral have been synthesized using ester, amide condensations, Wittig, Knoevenagel, Baylis-Hillman reactions and their antimicrobial activities were evaluated through disc diffusion and serial dilution methods. Several derivatives displayed interesting antimicrobial activity. Particularly, the Baylis-Hillman adducts 8-10 were found to have more potent antibacterial and antifungal activities than standard drugs namely chloramphenicol and nystatin respectively.

Influence of chlorine and methyl substituents and their position on the antimicrobial activities and crystal structures of 4-methyl-1,6-diphenylpyrimidine-2(1H)-selenone derivatives

2021 ◽  
Vol 77 (10) ◽  
Author(s):  
Izabela Korona-Głowniak ◽  
Wojciech Nitek ◽  
Waldemar Tejchman ◽  
Ewa Żesławska
Keyword(s):  
Crystal Structure ◽  
Biological Activity ◽  
Intermolecular Interactions ◽  
Current Knowledge ◽  
Antimicrobial Activities ◽  
Mutual Orientation ◽  
Antibacterial And Antifungal Activities ◽  
Chlorine Substituent ◽  
Antibacterial And Antifungal ◽  
Derivatives Of

Derivatives of 4-methyl-1,6-diphenylpyrimidine-2(1H)-selenone show very strong antimicrobial activity. In order to extend the current knowledge about the features responsible for the biological activity, crystal structure analyses are presented for 4-methyl-1-(2-methylphenyl)-6-phenylpyrimidine-2(1H)-selenone (1), 4-methyl-1-(3-methylphenyl)-6-phenylpyrimidine-2(1H)-selenone (2), 4-methyl-1-(4-methylphenyl)-6-phenylpyrimidine-2(1H)-selenone (3) (all C18H16N2Se) and 1-(4-chlorophenyl)-4-methyl-6-phenylpyrimidine-2(1H)-selenone (4) (C17H13ClN2Se). Furthermore, the antibacterial and antifungal activities of these compounds were evaluated. All the presented derivatives crystallize in the space group P21/c with one molecule in the asymmetric unit. The molecular geometries differ slightly in the mutual orientation of the rings. The packing of molecules in the crystals is dominated by C—H...N and C—H...Se intermolecular interactions. Additionally, in the crystal structure of 4, C—H...Cl intermolecular interactions are observed. The introduction of a methyl or chlorine substituent improves the biological activity, while its position significantly affects biological activity only in case of the chlorine substituent.

scholarly journals Synthesis and Antimicrobial Activity of Novel Benzoxazoles

2012 ◽  
Vol 67 (9-10) ◽  
pp. 466-472 ◽  
Author(s):  
Mustafa Arisoy ◽  
Ozlem Temiz-Arpaci ◽  
Fatma Kaynak-Onurdag ◽  
Selda Ozgen
Keyword(s):  
Antimicrobial Activity ◽  
Inhibitory Concentration ◽  
Antimicrobial Activities ◽  
Microbial Pathogens ◽  
Drug Resistant ◽  
Dilution Technique ◽  
Spectral Techniques ◽  
Antibacterial And Antifungal Activities ◽  
Antibacterial And Antifungal ◽  
New Compounds

A series of 2-(p-substituted-benzyl)-5-[[4-(p-chloro/fluoro-phenyl)piperazin-1-yl]ace tamido] -benzoxazoles were synthesized in need of new compounds for the fight against microbial pathogens. Their structures were elucidated by spectral techniques. These new derivatives, along with previously synthesized 2-(p-substituted-benzyl)-5-substituted-benzoxazoles, were evaluated for their antibacterial and antifungal activities against standard strains and drugresistant isolates in comparison with ampicillin, gentamicin sulfate, ofloxacin, vancomycin, fluconazole, and amphotericin B trihydrate. The minimum inhibitory concentration (MIC) of each compound was determined by a two-fold serial dilution technique. The compounds were found to possess a broad spectrum of antimicrobial activities with MIC values of 32 - 256 μg/ml. Although standard drugs were more active against the pathogenes employed in this study, the activities of the new benzoxazoles and reference drugs against drug-resistant isolates of the microorganisms were largely similar

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