The Association Between Knee Osteoarthritis and HLA-DRB1*0101 in the East of Iran

2020 ◽  
Vol 16 (2) ◽  
pp. 134-138
Author(s):  
Omid Kooshkaki ◽  
Elham Atabati ◽  
Majid Shayesteh ◽  
Fatemeh Salmani ◽  
Gholamreza A. Sarab
Keyword(s):  
Knee Osteoarthritis ◽  
Human Leukocyte ◽  
Inflammatory Factors ◽  
Control Group ◽  
Specific Primer ◽  
Leukocyte Antigen ◽  
Genetic Components ◽  
Bone Sclerosis ◽  
East Of Iran ◽  
Joint Trauma

Background: Osteoarthritis (OA) is a painful social problem, which breaks down the articular cartilage, causes the failure of synovial joints and subchondral bone sclerosis. OA etiology is not completely understood, but joint trauma, infection, obesity, and diseases are the most important risk factors for OA developing. Recent studies suggested inflammatory factors and genetic components can be involved in the pathogenesis of OA. Experimental evidences suggest a linkage between Human Leukocyte Antigen (HLA) genetic diversity and OA. But a few studies have been conducted in this subject. Objective: To investigate the association between HLA-DRB1*0101 and OA in Iranian patients. Methods: Thirty patients with knee osteoarthritis and 30 healthy people as the control group were included in the study. Sex, weight, age, Body mass index (BMI) and height of all participants were recorded. HLA-DRB1*0101 was typed by PCR using the sequence-specific primer. Results: Our results showed 80% of knee osteoarthritis patients were positively HLA-DRB1*0101 (n=24), while only 26.7% of controls were positive (n=8) (P= 0.015). Conclusion: These findings proposed that there is a significant association between HLADRB1* 0101 and susceptibility to knee osteoarthritis.

scholarly journals Treatment of Anti-HLA Donor-Specific Antibodies Results in Increased Infectious Complications and Impairs Survival after Liver Transplantation

2020 ◽  
Vol 9 (12) ◽  
pp. 3986
Author(s):  
Sinem Ünlü ◽  
Nils Lachmann ◽  
Maximilian Jara ◽  
Paul Viktor Ritschl ◽  
Leke Wiering ◽  
...  
Keyword(s):  
Liver Transplantation ◽  
Causes Of Death ◽  
Human Leukocyte ◽  
Infectious Complications ◽  
Control Group ◽  
Leukocyte Antigen ◽  
High Urgency ◽  
Donor Specific Antibodies ◽  
One Year ◽  
The Relationship

Donor-specific anti-human leukocyte antigen antibodies (DSA) are controversially discussed in the context of liver transplantation (LT). We investigated the relationship between the presence of DSA and the outcome after LT. All the LTs performed at our center between 1 January 2008 and 31 December 2015 were examined. Recipients < 18 years, living donor-, combined, high-urgency-, and re-transplantations were excluded. Out of 510 LTs, 113 DSA-positive cases were propensity score-matched with DSA-negative cases based on the components of the Balance of Risk score. One-, three-, and five-year survival after LT were 74.3% in DSA-positive vs. 84.8% (p = 0.053) in DSA-negative recipients, 71.8% vs. 71.5% (p = 0.821), and 69.3% vs. 64.9% (p = 0.818), respectively. Rejection therapy was more often applied to DSA-positive recipients (n = 77 (68.1%) vs. 37 (32.7%) in the control group, p < 0.001). At one year after LT, 9.7% of DSA-positive patients died due to sepsis compared to 1.8% in the DSA-negative group (p = 0.046). The remaining causes of death were comparable in both groups (cardiovascular 6.2% vs. 8.0%; p = 0.692; hepatic 3.5% vs. 2.7%, p = 0.788; malignancy 3.5% vs. 2.7%, p = 0.788). DSA seem to have an indirect effect on the outcome of adult LTs, impacting decision-making in post-transplant immunosuppression and rejection therapies and ultimately increasing mortality due to infectious complications.

scholarly journals Multiple sclerosis and human leukocyte antigen genotypes: Focus on the Middle East and North Africa region

2020 ◽  
Vol 6 (1) ◽  
pp. 205521731988177
Author(s):  
Zhila Maghbooli ◽  
Mohammad Ali Sahraian ◽  
Abdorreza Naser Moghadasi
Keyword(s):  
Multiple Sclerosis ◽  
Middle East ◽  
Human Leukocyte Antigen ◽  
North Africa ◽  
Human Leukocyte ◽  
Genetic Studies ◽  
Leukocyte Antigen ◽  
Genetic Components ◽  
Control Designs

Recent reports have demonstrated that the prevalence of multiple sclerosis (MS) is increasing in the Middle East and North Africa region. There is also emerging evidence regarding the genetic components of MS risk. This review provides an overview of the role of genetic factors in MS susceptibility by examining human leukocyte antigen loci in patients within the Middle East and North Africa region. Most of the genetic studies conducted in the Middle East and North Africa region have been based on case–control designs, which cannot confirm direct causality of genetic variants on MS susceptibility. Moreover, there are very limited and inconsistent studies on human leukocyte antigen class I and II (DQA and DQB) in MS patients of the Middle East and North Africa region. To identify common risk haplotypes in the Middle East and North Africa region or its sub-populations, further longitudinal studies will be required.

Validation of sensitive human leukocyte antigen-sequence-specific primer and probe typing in forensic DNA examination

2006 ◽  
Vol 8 (4) ◽  
pp. 203-209 ◽  
Author(s):  
Masao Ota ◽  
Kazunori Shimada ◽  
Hideki Asamura ◽  
Kayoko Takayanagi ◽  
Yoshihiko Katsuyama ◽  
...  
Keyword(s):  
Human Leukocyte Antigen ◽  
Human Leukocyte ◽  
Forensic Dna ◽  
Specific Primer ◽  
Leukocyte Antigen

scholarly journals HLA-B27 antigen frequency among suspected Spondyloarthropathy patients attaining a tertiary level hospital of Bangladesh

2015 ◽  
Vol 40 (3) ◽  
pp. 102-106 ◽  
Author(s):  
A Nessa ◽  
S Tabassum ◽  
S Sultana
Keyword(s):  
Healthy Subjects ◽  
Age Groups ◽  
Human Leukocyte ◽  
Disease Association ◽  
Control Group ◽  
Hla B27 ◽  
Leukocyte Antigen ◽  
Male Preponderance ◽  
Antigen Frequency ◽  
Medical University

Human leukocyte antigen B27 (HLA-B27), a class I molecules of the major histocompatibility complex has a strong disease association with different types of spondarthropathies (SpA). The strength of this disease association varies markedly among racial and ethnic populations. The present study aimed to identify the HLA-B27 antigen frequencies among suspected SpA patients as well as healthy Bangladeshi individuals. The frequency of HLA-B27 was determined in 1500 patients and 1000 healthy subjects attending the Bangabandhu Sheikh Mujib Medical University (BSMMU). HLAB 27 typing was done by microlymphocytotoxicity test using commercial kit. A total of 738 (49.2%) suspected SpA patients and 107 (10.7%) healthy subjects tested positive for HLA-B27 antigen with higher frequency among younger age groups (54.9%, 52.4% and 56.2% in 0-14 years, 15-24 years and 25–34 years of age respectively). The male female positivity was almost same (11.4% and 9.6%) among control group, but in patient group it was 53.0% and 41.2% respectively. The findings of this hospital based study showed a high frequency of HLA-B27 among suspected SpA patients with male preponderance which is comparable with neighboring countries.Bangladesh Med Res Counc Bull 2014; 40 (3): 102-106

Significance of Expression of miR-365, Cartilage Oligomeric Matrix Protein and Inflammatory Factors in Knee Osteoarthritis

2020 ◽  
Vol 10 (7) ◽  
pp. 1065-1069
Author(s):  
Ren Lin ◽  
Jinglai Xue ◽  
Junqin Qiu
Keyword(s):  
Knee Osteoarthritis ◽  
Cartilage Oligomeric Matrix Protein ◽  
Matrix Protein ◽  
Serum Levels ◽  
Inflammatory Factors ◽  
Control Group ◽  
Observation Group ◽  
Logistic Analysis ◽  
Tnf Α ◽  
And Gender

This study is to investigate the high expression of miR-365, cartilage oligomeric matrix protein (COMP) and inflammatory factors in knee osteoarthritis and their correlation with disease activity. The 94 patients with knee osteoarthritis were selected as the observation group. Controls were 94 healthy people matched according to the age and gender of the observation group. The expression levels of blood miR-365, cartilage oligomeric matrix protein, and the inflammatory IL-6 and TNF-α were compared between two groups and correlations between these indicators and Lysholm knee scores were determined. The serum levels of miR-365, cartilage oligomeric matrix protein and the inflammatory factors IL-6 and TNF-α in osteoarthritis patients exceeded those in the normal control group (P < 0 05), and miR-365 was positively correlated with cartilage oligomeric matrix protein and IL-6 and TNF-α (P < 0 05). In addition, the positive correlation was found between the Lysholm osteoarthritis score and the above factors (P < 0 05). Finally, logistic analysis of elevated peripheral blood miR-365 and COMP is an independent risk factor for osteoarthritis. MiR-365, COMP and inflammatory factors IL-6 and TNF-α are highly expressed in knee osteoarthritis.

MHC Class I related chain A (MICA), Human Leukocyte Antigen (HLA)-DRB1, HLA-DQB1 genotypes in Turkish patients with ulcerative colitis

2020 ◽  
Vol 45 (5) ◽  
pp. 587-592
Author(s):  
Cigdem Kekik Cinar ◽  
Kadir Demir ◽  
Sonay Temurhan ◽  
Filiz Akyuz ◽  
Binnur Pinarbasi ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Human Leukocyte Antigen ◽  
Mhc Class I ◽  
Patient Group ◽  
Human Leukocyte ◽  
Class I ◽  
Control Group ◽  
Leukocyte Antigen ◽  
Hla Genotypes ◽  
Turkish Patients

AbstractObjectivesWe aimed to determine Human Leukocyte Antigen (HLA)-DRB1, DQB1, and MHC Class I related chain A (MICA) genotypes in patients with ulcerative colitis.MethodsHLA-DRB1, HLA-DQB1, MICA genotyping of patient (n:85) and controls (n:100) were performed by PCR-SSO Luminex (One Lambda genotyping kit).ResultsWe found significantly higher DRB1*01 (p:0.022, OR:0.23, CI:0.06–0.8) and MICA*0002/20/55 (p:0.03, OR:0.53, CI:0.29–0.93) alleles in control group whereas DRB1*14 (p:0.04, OR:2.25, CI:1–5.08), DRB1*15 (p:<0.0001, OR:4.54, CI:2.09–9.88) and MICA*0004 (p:0.01, OR:2.84, CI:1.2–6.7) alleles were higher in patient group.ConclusionsThe present study will inform the MICA and HLA genotypes about the protective (DRB1*01, MICA*0002/20/55) or susceptible (DRB1*14, DRB1*15, MICA*0004) alleles of the disease and helps the literature on Turkish patients with ulcerative colitis.

scholarly journals HLA Polymorphisms and Risk of Glioblastoma in Koreans

2021 ◽  
Author(s):  
Stephen Ahn ◽  
Haeyoun Choi ◽  
In-Cheol Baek ◽  
Soon A. Park ◽  
Yeo Song Kim ◽  
...  
Keyword(s):  
Human Leukocyte ◽  
Hla Class I ◽  
Class I ◽  
Control Group ◽  
Hla Association ◽  
Hla Polymorphism ◽  
Leukocyte Antigen ◽  
Cancer Types ◽  
Control Study

Purpose Immune responses for cancer cells can be altered according to genetic variation of human leukocyte antigen (HLA). Association of HLA polymorphism with risk of various cancer types is well known. However, the association between HLA and glioblastoma (GBM) remains uncertain. We sought to evaluate the association of HLA polymorphism with risk of GBM development in Koreans. Materials and Methods A case-control study was performed to identify the odds ratios (OR) of HLA class I and II genes for GBM. The control group consisted of 142 healthy Korean volunteers, and the GBM group was 80 patients with newly diagnosed GBM at our institution. HLA class I (-A, -B, and &ndash;C) and class II (-DR, -DQ, and &ndash;DP) genotyping was performed by high-resolution polymerase chain reaction (PCR)-sequence-based typing (PCR-SBT) methods. Results There were significantly decreased frequencies of HLA-A*26:02 (OR 0.22 CI 0.05-0.98), HLA-C*08:01 (OR 0.29 CI 0.10-0.87), and HLA-DRB1*08:03 (OR 0.32 CI 0.11-0.98), while there was significantly increased frequency of HLA-C*04:01 (OR 2.29 CI 1.05-4.97). In analysis of haplotypes, the frequency of DRB1*14:05-DQB1*05:03 was significantly decreased (OR 0.22 CI 0.05-0.98). Conclusion This study suggests that genetic variations of HLA may affect GBM development in Koreans. Further investigations with larger sample sizes are needed to delineate any potential role of the HLA polymorphisms in the pathogenesis of GBM development.

scholarly journals The Relationship between the HLA-G Polymorphism and sHLA-G Levels in Parental Pairs with High-Risk Pregnancy

2019 ◽  
Vol 16 (9) ◽  
pp. 1546 ◽  
Author(s):  
Olimpia Sipak ◽  
Aleksandra Rył ◽  
Anna Grzywacz ◽  
Maria Laszczyńska ◽  
Małgorzata Zimny ◽  
...  
Keyword(s):  
Genomic Dna ◽  
Human Leukocyte ◽  
Control Group ◽  
Polish Population ◽  
Leukocyte Antigen ◽  
Risk Pregnancy ◽  
Human Leukocyte Antigen G ◽  
The Relationship ◽  
Class Ib

Human leukocyte antigen G (HLA-G) is observed in immune system cells and other organs. It is a class Ib molecule, which plays a pivotal role in the implantation and maintenance of pregnancy. The aim of this study was to assess the relationship between serum sHLA-G levels and the HLA-G allele in parental pairs with complicated obstetric histories. The clinical material consisted of 210 women and 190 men with the experience of a complicated or an unsuccessful pregnancy. The control group included parents―89 women and 86 men―lacking complicated obstetric histories. We applied genetic analysis methods: isolation of genomic DNA, sequencing, and determination of serum sHLA-G levels. There were no statistically significant differences in the frequencies of the HLA-G −725 C>G polymorphism between particular experimental groups compared with the control group (p > 0.05). The median sHLA-G levels in the women with the HLA-G10101 allele (15.4 U/mL) were significantly higher than in the women with other alleles (p < 0.05). The HLA-G 10101 allele seems to protect against antiphospholipid syndrome, which may be associated with increased serum sHLA-G levels in its carriers. The relationship between serum sHLA-G levels and the HLA-G polymorphisms in the Polish population requires further investigation.

scholarly journals Molecular Analysis of HLA-G in Women with High-Risk Pregnancy and Their Partners with Regard to Possible Complications

2019 ◽  
Vol 16 (6) ◽  
pp. 982 ◽  
Author(s):  
Olimpia Sipak ◽  
Aleksandra Rył ◽  
Anna Grzywacz ◽  
Maria Laszczyńska ◽  
Sławomir Szymański ◽  
...  
Keyword(s):  
Human Leukocyte ◽  
Control Group ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Leukocyte Antigen ◽  
Risk Pregnancy ◽  
Polymerase Chain ◽  
Complicated Pregnancy ◽  
Human Leukocyte Antigen G ◽  
G 14

The understanding of the molecular and biochemical characteristics of the human leukocyte antigen-G (HLA-G) is important because of the diverse influence of this antigen’s polymorphisms on the course of a pregnancy. The aim of our study was to assess how the variation of the HLA-G allele and the HLA-G 14-bp ins/del polymorphism influence predisposition to a complicated pregnancy. The clinical material consisted of parental pairs with complicated pregnancies (210 women; 190 men). The control group included parental pairs without complications during pregnancy (89 women; 86 men). The study involved isolation of genome DNA from peripheral blood leukocytes, sequencing, and analysis of the 14-bp ins/del polymorphism in the 3′-untranslated region (3′-UTR) of the HLA-G gene based on polymerase chain reaction (PCR). The most common HLA-G allele in the group of women with complicated pregnancies was the HLA-G 10101 allele. There were no statistically significant differences in the frequencies of the 14-bp ins/del polymorphism in the 3′UTR of the HLA-G gene between the groups. Our results suggest that the risk of complications in pregnancy is influenced by the HLA-G 10101, HLA-G 10108, and HLA-G 10106 alleles and is not influenced by the 14-bp ins/del polymorphism in the 3′UTR of the HLA-G gene.

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