Effects of Beer and Lettuce (Lactuca Sativa) Consumption on Antioxidant Status in Healthy Volunteers: A Small-Scale Crossover Trial

Several disorders of amino acid (AA) metabolism are treated with a protein-restricted diet supplemented with specific AA mixtures. Delivery kinetics impacts AA absorption and plasma concentration profiles. We assessed plasma profiles after ingestion of an AA mixture engineered to prolong AA absorption with Physiomimic TechnologyTM (Test) in a randomized, single-dose, four-way crossover trial in healthy volunteers (Trial Registration: ISRCTN11016729). In a two-step hypothesis, the primary endpoints were (i) significant reduction in peak plasma concentrations (Cmax) of essential amino acids (EAAs) while (ii) maintaining EAA bioavailability (AUC0-300 min) compared to a free AA mixture (Reference). Secondary endpoints included effects on plasma profiles of other AA groups and effects on several metabolic markers. Thirty subjects completed the study. Both co-primary endpoints were met: Cmax for EAAs was 27% lower with the Test product compared to the Reference product (ratio, 0.726, p < 0.0001); overall plasma EAA levels from the two AA mixtures was within the pre-specified bioequivalence range (AUC0-300min ratio, 0.890 (95% CI: 0.865, 0.915)). These findings were supported by the results of secondary endpoints. Prolongation of AA absorption was associated with modulation of several metabolic markers. It will be important to understand whether this can improve the long-term management of disorders of AA metabolism.

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Higher bioavailability of isoflavones after a single ingestion of a soya-based supplement than a soya-based food in young healthy males

British Journal Of Nutrition ◽

10.1017/s0007114507803953 ◽

2008 ◽

Vol 99 (2) ◽

pp. 333-344 ◽

Cited By ~ 25

Author(s):

Sébastien Vergne ◽

Catherine Bennetau-Pelissero ◽

Valérie Lamothe ◽

Philippe Chantre ◽

Mylène Potier ◽

...

Keyword(s):

Healthy Volunteers ◽

Crossover Trial ◽

Human Subjects ◽

Biologically Active ◽

Pharmacokinetic Parameters ◽

Relative Importance ◽

Elisa Method ◽

Isoflavone Content ◽

Derivative Products ◽

Healthy Males

Soya isoflavones, genistein and daidzein, are the focus of numerous studies investigating their potential effects on health and results remain controversial. Bioavailability is clearly a crucial factor influencing their bioefficacy and could explain these discrepancies. This study aimed at assessing: (1) the isoflavone content of sixty-nine European soya-derivative products sold on the French market; (2) the bioavailability of isoflavones comparing supplement with food. Twelve healthy volunteers were recruited in a randomized two-way crossover trial and received 35 mg isoflavones equivalent aglycone either through supplements or through cheese, both containing different patterns of isoflavone conjugates and different daidzein:genistein ratios. A specific ELISA method was used to assess the plasma and urinary concentrations of isoflavones and thus the pharmacokinetic parameters, which were then normalized to mg of each isoflavone ingested. Results showed that the normalized Cmax of daidzein (P = 0·002) and similarly the normalized AUC0 → ∞andCmaxof genistein (P = 0·002) from soya-based capsules were higher than that from soya-based cheese. In conclusion, this work completes studies on isoflavone bioavailability and presents new data regarding isoflavone concentrations in soya-derivative products. Assuming that isoflavone conjugation patterns do not influence isoflavone bioavailability, this study shows that isoflavones contained in capsules are more bioavailable than those contained in soya-based cheese. Although the supplement is more bioavailable, the relative importance of this is difficult to interpret as there is little evidence that supplements are biologically active in human subjects to date and further studies will be necessary for this specific supplement to prove its efficacy.

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Inhibition of Platelet Aggregation in Man by Indobufen (K 3920)

Journal of International Medical Research ◽

10.1177/030006058100900103 ◽

1981 ◽

Vol 9 (1) ◽

pp. 12-17 ◽

Cited By ~ 6

Author(s):

A Vittoria ◽

F Laghi Pasini ◽

G L Messa ◽

T Di Perri ◽

G Corvi ◽

...

Keyword(s):

Platelet Aggregation ◽

Healthy Volunteers ◽

Crossover Trial ◽

Marked Effect ◽

Threshold Concentration ◽

Dose Effect ◽

Inhibition Of Platelet Aggregation ◽

Oral Doses

A complete crossover trial was undertaken in six healthy volunteers to gain information on dose-effect responses to indobufen by assessing the intensity and duration of the effect of 3 single oral doses of the drug on platelet aggregation induced by threshold concentration of ADP and by 3 added doses of collagen. The results of the study confirm that the activity is dose-related and is reversible since 24 hours after administration it has practically disappeared. The effect of the same dose of indobufen differed significantly according to the amount of collagen added to plasma, whereas increasing doses of indobufen provoked a significantly more marked effect when the amount of inducer employed was the same.

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Comparative study of electronic vs. paper VAS ratings: a randomized, crossover trial using healthy volunteers

Pain ◽

10.1016/s0304-3959(02)00178-1 ◽

2002 ◽

Vol 99 (1) ◽

pp. 341-347 ◽

Cited By ~ 124

Author(s):

Robert N. Jamison ◽

Richard H. Gracely ◽

Stephen A. Raymond ◽

Jonathan G. Levine ◽

Barbara Marino ◽

...

Keyword(s):

Comparative Study ◽

Healthy Volunteers ◽

Crossover Trial ◽

Randomized Crossover Trial

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Effects of Single Oral Doses of Gemifloxacin (320 Milligrams) versus Trovafloxacin (200 Milligrams) on Fecal Flora in Healthy Volunteers

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.45.2.608-611.2001 ◽

2001 ◽

Vol 45 (2) ◽

pp. 608-611 ◽

Cited By ~ 9

Author(s):

Gloria Garcia-Calvo ◽

Ana Molleja ◽

Maria J. Giménez ◽

Araceli Parra ◽

Eva Nieto ◽

...

Keyword(s):

Escherichia Coli ◽

Healthy Volunteers ◽

Washout Period ◽

Crossover Trial ◽

Fecal Flora ◽

Randomized Crossover Trial ◽

The Family ◽

Stool Samples ◽

Resistant Strains ◽

Oral Doses

ABSTRACT Gemifloxacin and trovafloxacin were administered to 12 volunteers in a randomized crossover trial with a 2-week washout period. Stool samples were collected predose and 1, 2, and 3 days postdose. Both quinolones reduced the number of organisms of the familyEnterobacteriaceae and aerobic gram-positive organisms. Escherichia coli reduction was greater with gemifloxacin than with trovafloxacin, with postdose isolation of quinolone-resistant strains for which MICs of trovafloxacin were higher than those of gemifloxacin.

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Elucidating the genetic basis of antioxidant status in lettuce (Lactuca sativa)

Horticulture Research ◽

10.1038/hortres.2015.55 ◽

2015 ◽

Vol 2 (1) ◽

Cited By ~ 13

Author(s):

Annabelle Damerum ◽

Stacey L Selmes ◽

Gaia F Biggi ◽

Graham JJ Clarkson ◽

Steve D Rothwell ◽

...

Keyword(s):

Lactuca Sativa ◽

Antioxidant Status ◽

Genetic Basis

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A Study of the Effectiveness of Hydroponic Growing Variables on Lactuca sativa var. capitata

Journal of Student Research ◽

10.47611/jsrhs.v10i2.1506 ◽

2021 ◽

Vol 10 (2) ◽

Author(s):

Jacob Zajkowski ◽

Whitney Short

Keyword(s):

Deep Water ◽

Lactuca Sativa ◽

Nutrient Concentration ◽

Growth Efficiency ◽

Nutrient Concentrations ◽

Small Scale ◽

Controlled Environment ◽

Water Culture ◽

Future Studies ◽

System Variables

Hydroponic growing in controlled environment horticulture has been an increasingly used method of produce production around the world. Its many methods integrate sustainability and growth efficiency through the control of climatic and system variables. This study investigated control variables that would produce and market a more effective lettuce (lactuca sativa. var capitata) crop. Three objectives determined: The comparison between the dimensions of deep water culture systems and the lettuce harvesting length, Consumer and ICP spectrometry recognition of different post-harvest hydroponic nutrient concentration, and the preference of lettuce grown in different hydroponic nutrient concentrations. Through growing trials and consumer tests, it was found that the 14 gallon (102 x 50.8 x 66cm) size deep water culture system also produced lettuce with larger harvest-length; spectrometry recognition of lettuce crops grown in different nutrient levels was effective with 7 of the 12 nutrients elements showing sufficient results of concentration in concentrated lettuce. Consumer identification recognition wasn’t successful with 40% of consumers unable to recognize any nutrient concentration level compared to two other varieties. Representing a diverse market audience, of consumers determined that variety 127 (50% regular concentration) was preferred as significant market influence of purchase. This research will impact future studies in effective small scale hydroponic growing and growers looking to expand knowledge of beneficial growth.

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