The Antioxidant Potential of Black Cumin (Nigella sativa L.) Extracts Through Different Extraction Methods

2020 ◽  
Vol 15 (6) ◽  
pp. 623-630
Author(s):  
Muhammad J. Iqbal ◽  
Masood S. Butt ◽  
Muhammad Sohail ◽  
Hafiz A.R. Suleria
Keyword(s):  
Supercritical Fluid ◽  
Methanolic Extract ◽  
Nigella Sativa ◽  
Extraction Methods ◽  
Antioxidant Potential ◽  
Time Intervals ◽  
Black Cumin ◽  
Therapeutic Benefits ◽  
Bioactive Component ◽  
Better Than

Background: Herbal foods possess significant amounts of essential bioactive nutrients and offer clinical and therapeutic benefits. The aim of present research was to compare the antioxidant potential of black cumin extracts obtained using different extraction modes. Materials and Methods: Three solvents (methanol, acetone and hexane) were employed at different time intervals and ratios. However, Supercritical Fluid Extracts (SFE) were obtained at varying pressures (5500, 6500 and 7500 psi). Results: Outcomes revealed that total phenolics were maximum in methanolic extract (790.79±31.67 mg GAE/100 g) at 50 min. Similarly, DPPH and FRAP activity of methanolic extract was also better than other extracts as 80.99±4.02% and 27.44±0.88 mg TE/g, respectively. HPLC quantification exhibited that highest recovery of major bioactive component (thymoquinone) was obtained via supercritical extraction (12.38 mg/g) at 7500 psi. Conclusions: It is concluded that Pakistani variety of black cumin possess appreciable antioxidant activity which directly depends upon the extraction conditions. Among conventional solvents, methanol was evidenced to be more efficient. However, supercritical fluid extracts have proved a reliable mean for the extraction of thymoquinone.

Physicochemical and Antioxidant Properties of Pizza Dough-base Enriched with Black Cumin (Nigella sativa) Extracts

2019 ◽  
Vol 15 (5) ◽  
pp. 508-516
Author(s):  
Muhammad J. Iqbal ◽  
Masood S. Butt ◽  
Iqra Saeed ◽  
Hafiz A.R. Suleria
Keyword(s):  
Phenolic Content ◽  
Therapeutic Potential ◽  
Nigella Sativa ◽  
Antioxidant Properties ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Pharmacological Activities ◽  
Black Cumin ◽  
Ancient Times ◽  
Better Than

Background: Changing lifestyles have paved the way towards various physiological dysfunctions. Phytochemicals derived from spices are being widely utilized in diet from ancient times to fight against these physiological dysfunctions owing to their therapeutic potential and high pharmacological activities. Methods: Methods: The current investigation was an attempt to explore the antioxidant potential, physicochemical, and sensory properties of black cumin (Nigella sativa) enriched pizza base that was developed by using conventional and supercritical extracts. Results: It was observed that the texture of product becomes harder during storage from 4.36±0.16 to 4.71±0.17 kg force. Nonetheless, supercritical extract pizza base got better hedonic scores compared to other treatments. Antioxidant potential of pizza base enriched with supercritical extracts (Total Phenolic Content 108.08±4.88 mg GAE/100g) was also better than control and conventional solvent extract enriched treatment as 63.24±3.03 and 95.34±3.66 mg GAE/100g, respectively. Conclusion: Conclusively, the results depicted that pizza base containing supercritical extract exhibited superior physiochemical, hedonic and antioxidant properties.

Investigating the Antioxidant Potential of Garlic (Allium sativum) Extracts Through Different Extraction Modes

2019 ◽  
Vol 15 (1) ◽  
pp. 45-50 ◽  
Author(s):  
Kanza A. Awan ◽  
Masood S. Butt ◽  
Iahtisham Ul Haq ◽  
Hafiz A.R. Suleria
Keyword(s):  
Allium Sativum ◽  
Total Phenolics ◽  
Methanolic Extract ◽  
Antioxidant Potential ◽  
Flavonoid Content ◽  
Time Intervals ◽  
Methanolic Extracts ◽  
Maximum Extraction ◽  
Acid Potential ◽  
The Ideal

Background: Garlic (Allium sativum) possesses health enhancing abilities due to the presence various phytoceutics moities. The current research was deigned to explore the phytochemicals and antioxidant capacity of Pakistani garlic. Methods: Garlic extracts were obtained using methanol, hexane and ethyl acetate at different time intervals (35, 50 and 65 min) followed by their polyphenols and flavonoid content determination. Afterwards, the antioxidant potential was also determined. Results: The outcomes revealed that the methanolic extracts obtained at 50 min extraction time showed maximum total phenolics as 60.38±0.23 mg GAE/100g and flavonoids as 58.45±1.24 mg/100g. Similarly, the highest DPPH activity (61.59±1.58%) and β-carotene and linoleic acid potential (64.96±1.72%) were also observed for methanolic extract. Conclusion: Inferences were made that Pakistani garlic contains myriad of phenolics and flavonoids but the extraction of these components depends upon the solvent/time combination. In this study, methanol proved to be the ideal solvent for the maximum extraction of phytochemicals from garlic.

scholarly journals Optimization of Microwave-assisted Extraction of Thymoquinone from Nigella sativa L. Seeds

2016 ◽  
Vol 35 (2) ◽  
pp. 209 ◽  
Author(s):  
Erkan Karacabey
Keyword(s):  
Nigella Sativa ◽  
Extraction Methods ◽  
Microwave Assisted Extraction ◽  
Black Cumin ◽  
Solid Ratio ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Black Cumin Seed ◽  
Solid Liquid ◽  
Cumin Seed

The high potential of thymoquinone as an ingredient and/or additive in the food, pharmaceutical and cosmetic industries has been well established in previous studies. However, its extraction from natural sources was considered in the limited studies and none of them included the microwave-assisted extraction (MAE) of a thymoquinone-rich extract and process optimization. In the present study, this high-value-added bioactive was aimed to extract from its well-known natural source, black cumin seed (<em>Nigella sativa</em> L.), using methanol as a solvent for all of the studied extraction methods. For extraction of a compound of interest, microwave-assisted extraction system having temperature controlling function was used and its performance was compared with common extraction methods, Soxhlet and conventional solid/liquid extraction. The results indicated that the MAE system provided a rich extract containing thymoquinone, which was 2 and 7 times higher than those produced by conventional solid/liquid extraction and Soxhlet, respectively. Influences of temperature, time and solvent/solid ratio on thymoquinone yield were investigated for MAE. The solvent/solid ratio was found to have the main effect on extraction performance, whereas an interaction effect of temperature and time was significant. Variables of MAE were optimized by response surface methodology to produce a thymoquinone-rich extract. Optimal conditions for the highest yield of thymoquinone were determined as 10 minutes extraction at 30 °C, using 30 ml solvent per gram of black cumin seed. The estimated thymoquinone yield of the extract was 628 mg/kg black cumin seed. It could be concluded that the currently optimized MAE with temperature controlling function is a promising technique to produce a thymoquinone-rich extract from black cumin seeds.

Antimalarial and antioxidant activities of methanolic extract of Nigella sativa seeds (black cumin) in mice infected with Plasmodium yoelli nigeriensis

2010 ◽  
Vol 108 (6) ◽  
pp. 1507-1512 ◽  
Author(s):  
Valeelat O. Okeola ◽  
Oluwatosin A. Adaramoye ◽  
Chiaka M. Nneji ◽  
Catherine O. Falade ◽  
E. Olatunde Farombi ◽  
...  
Keyword(s):  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Nigella Sativa ◽  
Black Cumin

scholarly journals Nigella sativa – a Plant with Personality in Biochemistry and Experimental Medicine Researches

2016 ◽  
Vol 73 (2) ◽  
pp. 203
Author(s):  
Mirela Ahmadi ◽  
Mihaela Scurtu ◽  
Camelia Tulcan ◽  
Oana Maria Boldura ◽  
Cornelia Milovanov ◽  
...  
Keyword(s):  
Seed Oil ◽  
Nigella Sativa ◽  
Experimental Medicine ◽  
Black Cumin ◽  
Bioactive Component ◽  
Medical Benefits ◽  
Oil Extract ◽  
Neural Disorders ◽  
Inflammatory Action ◽  
Seed Extracts

Last decades often presented references to traditional medicine, or culinary use of natural resources for a better health status, prevention or treatment of different diseases. One of the natural plants came lately in the researches as a miracle salve: Nigella sativa. Also commonly known as black cumin, the most use of this plant are the seeds, such as, as powder, as oil extract, or as hydro or alcoholic extracts. Culinary usage of N. sativa is referring to the seeds used as spice. Medical usage of this plantis mostly used as oil or extracts administrated orally or intraperitoneal. The best demonstrated bioactive component is thymoquinone, an alkaloid, monotherpenoid compound, that seems to be the key of medical benefits of N. sativa. Experimental medicine proved that seeds of black cumin have health enhancement and pharmaceutical effects, being used in various disseases (cardiac, digestive and respiratory diseases; hepatic and renal tonic; inflammations; reproductive and neural disorders, analgesic; appetite stimulant; cancer prevention and treatment; spasmolytic and diabetes. Seeds (as powder, oil or hydro, alcoholic extracts) have been demonstrated that have antimicrobial, antiparasitic, antitumoral, antidiabetic, antioxidant, and anti-inflammatory action. N. sativa is easy to cultivate, storing, and offering diver potential of use as seed, seed oil, different type of seed extracts.

scholarly journals Methanolic Extract of Nigella sativa Seed Inhibits SiHa Human Cervical Cancer Cell Proliferation Through Apoptosis

2013 ◽  
Vol 8 (2) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Tarique N. Hasan ◽  
Gowhar Shafi ◽  
Naveed A Syed ◽  
Muhammad A Alfawaz ◽  
Mohammed A. Alsaif ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cancer Cells ◽  
Methanolic Extract ◽  
Nigella Sativa ◽  
Alternative Source ◽  
Inhibition Of Proliferation ◽  
Black Cumin ◽  
Cervical Cancer Cells ◽  
Human Cervical Cancer Cells ◽  
Human Cervical Cancer

Nigella sativa (NS), also known as black cumin, has long been used in traditional medicine for treating various cancer conditions. In this study, we sought to investigate the potential anti-cancer effects of NS extract using SiHa human cervical cancer cells. NS showed an 88.3% inhibition of proliferation of SiHa human cervical cancer cells at a concentration of 125 μL/mL methanolic extract at 24 h, and an IC50 value 93.2 pL/mL. NS exposure increased the expression of caspase-3, -8 and -9 several-fold. The analysis of apoptosis by DeadEnd terminaltransferase-mediated dUTP-digoxigeninend labeling (TUNEL) assay was used to further confirm that NS induced apoptosis. Thus, NS was concluded to induce apoptosis in SiHa cell through bothp53 and caspases activation. NS could potentially be an alternative source of medicine for cervical cancer therapy.

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