Changes in essential oil profile of Thymus daenensis Celak., Thymus fedtschenkoi Ronniger., and Thymus vulgaris L. under methyl jasmonate treatment

Author(s):  
Alireza Shayganfar ◽  
Davod Akhzari

Background: Thymus plants are well-known medicinal plants and it is believed that the pharmaceutical and therapeutically properties of these plants are related to their essential oils. The quality and quantity of the essential oils, as a secondary metabolite of aromatic plant, is directly related to the physiological state of the plant. The role of jasmonates in the plant as signal molecules in mediation and up-regulation of plant defense and secondary metabolism processes is well recognized. Objective: With the aim of increasing the performance and stimulating secondary metabolites, this study evaluate the influence of foliar application of MJ on essential oil content and composition of three different Thymus species, whether as an elicitor or an activator. Method: The experiment was arranged in a randomized block design with MJ treatments in four levels (0, 30, 60, 100 mM) and three replications. Results: Compared to the control, essential oil content of all three species increased in all treatment levels. However, the changes of essential oil composition were different. Under MJ treatments, the amount of sesquiterpenes (especially caryophyllene oxide) increased in T. daenensis and T. fedtschenkoi. In addition, the amount of thymol in T. daenensis, thymol, and γ-terpinene in T. vulgaris increased, whereas carvacrol methyl ether in T. daenensis and p-cymene in T. vulgaris decreased. Conclusion: It seems the type of plant species has a specific role in determining the response. There were no interpretable changes between treatment levels.

2014 ◽  
Vol 6 (4) ◽  
pp. 505-508
Author(s):  
Prshang KHALILI ◽  
Reza AMIRNIA

Dracocephalum moldavica L. of the Labiatae family is an annual herbaceous plant. In Iran it is known as Badrashbi and Badrashboo. Recent publications have reported antibacterial and antimicrobial properties of its essential oil, which have a wide usage in industry. In this research, the effects of iron foliar application and different yields’ harvest times on the essential oil content and some morphological treats (plant height, branch number, fresh and dry weights, essential oil content and leaf Iron content) were investigated under experimental study in faculty of Agriculture of Urmia University during 2012. A factorial experiment in a randomized complete block design with three replications was used in the present study. Results showed that foliar application of iron had a significant effect on branch number, fresh and dry weight, biomass and oil percentage. Similarly, harvest time had a significant influence on fresh and dry weight, essential oil content, essential oil yield and leaf iron content, but its effect was not significant on stem diameter and branch number. The best harvest time for Dracocephalum moldavica L. was at 100% of flowering and 6 ml/l of iron application showed the best results in all characters measured.


2019 ◽  
pp. 1-5
Author(s):  
Eray Tulukcu

This study was conducted to determine the essential oil composition of some medicinal plants from herbalists that local community have interest in; The research was carried out in the Medicinal Plants Laboratory of  Konya, in 2014 – 2015. The medicinal plants used in the research were supplied by herbalists and wholesalers who supply medicinal plants in Konya. At the end of this study, essential oil composition of medicinal plants ranged from 0.71 % (Daphne) to 5% (Thyme). The lavender essential oil composition ranged between 2% and 5%, while the composition of thyme essential oil ranged between 1% and 5%. There are several reasons for the change observed in the essential oil content of medicinal plants. In general, the effect of difference in the proportion of essential oils of the medicinal plants used in the study was as a result of the difference in regions, domestically and abroad where these plants were collected.


2021 ◽  
Author(s):  
Rezaei Nazari Mahmonir ◽  
Vahid Abdossi ◽  
Zamani Hargalani Fariba ◽  
Kambiz Larijani

Abstract Background: In terms of medicinal and therapeutic properties, H. perforatum is the important species. However, Selenium has been applied as an alleviation strategy subjected to producing essential oils and biomass.Method: For this study, a randomized complete block design with three replications was used so that each experimental unit comprised of 30 number 8 pots. The treatments included the foliar application of selenium (6, 8, 10, and 12 mg/l), nano-selenium (6, 8, 10, and 12 mg/l), and control (distilled water), applied at the rosette stage and harvesting at 50% flowering stage.Results: The results of the analysis of variance showed that the effect of selenium and nano selenium fertilizers was significant (p<0.01) on plant height, inflorescence length, number of inflorescences, inflorescence weight, shoot weight, root length, the total weight of biomass, essential oil percentage, the content of hypericin and hyperforin, the selenium accumulation in the plant, relative leaf water content, the content of chlorophylls a, b, and total, the content of phenol, the content of proline, production and accumulation of catalase, peroxidase, malondialdehyde, and DPPH enzymes. The highest inflorescence weight (21 g/plant), shoot weight (70 g/plant), and essential oil content (0.14%) were obtained from the control treatment. The highest accumulation of hypericin (3.8 mg/g dry matter) was obtained from the foliar application of 8 mg/l selenium. The maximum accumulation of hyperforin (57 mg/g dry matter) was obtained from the foliar application of 10 mg/l selenium. The highest accumulation of selenium (287.52 μg/g dry matter) was obtained in the foliar application of 12 mg/l nano-selenium.Conclusion: If the purpose of producing H. perforatum is to use the shoots and essential oils of the plant, then the use of selenium and nano-selenium is not recommended at all and should not be used. If the goal is to produce high hypericin, high hyperforin, and also the accumulation of selenium in the plant, the use of treatments of 6 and 8 mg/l of selenium and nano-selenium could be applied.


2009 ◽  
Vol 64 (9-10) ◽  
pp. 611-614 ◽  
Author(s):  
Fathy M. Soliman ◽  
Miriam F. Yousif ◽  
Soumaya S. Zaghloul ◽  
Mona M. Okba

Z. Naturforsch. 64 c, 611 - 614 (2009); received March 1/30, 2009 The hydrodistilled essential oil content of Origanum majorana L. (Lamiaceae) ranged from 2.5 - 3% with the maximal value (3%) in spring. Analysis of the oil by GC/MS resulted in the identifi cation of 15, 15, 11, and 14 components in the oils prepared in spring, summer, autumn, and winter, respectively. The composition of the essential oils differed quantitatively and qualitatively according to the time of collection. Thymol (38.4%) and cis-sabinene hydrate (25.3%) were the major components in spring plants. Terpinen-4-ol (37.4%, 20.5%, 16.3%) was a major component in the summer, autumn and winter oils, respectively. cis- Sabinene hydrate (54.4%) was major in winter plants while terpinolene (43.1%) was the main component in autumn plants. Other components detected in lower amounts in all oil samples were sabinene and p-cymene (up to 7.4% and 13.9% in autumn), and α-terpinene (up to 13.3% in summer)


Agronomy ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1285
Author(s):  
Asma Bastami ◽  
Reza Amirnia ◽  
R. Z. Sayyed ◽  
Hesham A. El Enshasy

The quantitative yield and essential oil percentage and composition of two important savory species in response to various fertilizers were explored in a field experiment as a factorial study based on a randomized complete block design with three replications in north Lorestan, Iran, in 2017–2019. The first factor was assigned to three mycorrhizal fungi (Funneliformis mosseae, Rhizophagus irregularis, and Glomus fasciculatum), phosphate biofertilizer (Baravar-2), fish manure (800 kg/ha), cattle manure (20 t/ha), vermicompost (5 t/ha), and a control (no fertilization); the second factor was assigned to two savory species, including Satureja khuzestanica and S. rechingeri. The results of the combined analysis of variance for the second and third years showed that the simple effects of fertilizers and species were significant on all recorded traits, except for some constituents of the essential oil. Among the mycorrhizal fungi, R. irregularis and S. khuzestanica outperformed S. rechingeri in all traits, except for essential oil content and yield. The interaction between year and species was significant for all traits. The essential oil content of S. rechingeri in the third year (5.1%) was 18% higher than that of S. rechingeri in the second year (4.3%) and 41% higher than that of S. khuzestanica in the third year (3.6%). According to the results, the foliar application of vermin compost at a rate of 5 t/ha can contribute to the sustainable production of both savory species, improving their growth and essential oil yield.


2014 ◽  
Vol 16 (2) ◽  
pp. 240-251 ◽  

<div> <p>In recent years, there has been growing concern regarding undesirable side effects of synthetic antimicrobial drugs used for food preservation or in medicine and an explosive global spreading of multidrug resistant microbes are considered as a substantial global health threat. This necessitates the searching for new classes of safe and more effective antimicrobial agent by acting with different mechanisms. This study aimed to determine the antimicrobial activity of essential oils of some herbs which are endemic Iranian plants using minimum inhibitory (MIC) concentration of their essential oils. Results obtained from minimum inhibitory concentration showed that the essential oil of <em>Thymus vulgaris</em>, compared to other extracts, possess the best inhibitory effect in the lowest concentration. The extracts of <em>Artemisia kermanensis</em>, <em>Lavandula officinalis</em>, <em>Rosemarinous officinalis </em>and <em>Eucalyptus caesia </em>are reported to have inhibitory effect on <em>Pectobacterium carotovorum</em>.</p> </div> <p>&nbsp;</p>


2014 ◽  
Vol 62 ◽  
pp. 526-532 ◽  
Author(s):  
Sajad Heidari ◽  
Majid Azizi ◽  
Forouzandeh Soltani ◽  
Javad Hadian

Author(s):  
N. Pryvedeniuk ◽  
T. Kutsyk ◽  
L. Hlushchenko

The impact of nutrition area on Thymus vulgaris L. accumulation of essential oils in dry raw materials (Thymiherba) was studied and described in the article. Four schemes of cultivation were chosen to be studied: 45×40 cm — 56 thousand plants/ha, 45×30 cm — 74 thousand plants/ha, 45×20 cm — 111 thousand plants/ ha, 45×10 cm — 222 thousand plants/ha. It was shown that the increase of nutrition area helped to increase the content of essential oils in dry raw materials. Dry thyme grass with the highest content of essential oil 21.3 ml/kg was obtained when variants with the largest plant nutrition area were applied (the schemes of cultivation 45×40 cm — 56 thousand plants/ha). Reduction of the nutrition area decreased the essential oil content. According to the scheme of cultivation 45×30 cm (74 thousand plants/ha) content decreased to 20.8 ml/kg, and for the minimal nutrition area 45×10 cm (222 thousand plants/ha) the content of essential oil was 18.5 ml/kg. It was confirmed that main part of the essential oil in the raw thyme is contained in leaves, flowers and small sprouts. At the same time the woody stems contain essential oil only in trace amounts. According to the cultivation scheme 45x10 cm (222 thousand plants/ha) the leaves contained 34.2 ml/kg of essential oil, when the stems only 2.1 ml/kg. When the area of plant nutrition was increased the quality of raw materials slightly improved — for the scheme of cultivation 45x30 cm (74 thousand plants/ha) leaves contained 35.9 ml/kg, stems — 2.1 ml/kg of essential oil. The content of essential oil in the leaves was the highest — 36.2 ml/kg while using the scheme with maximum researched plant nutrition area 45×40 cm (56 thousand plants / ha). The efficiency of four norms of basic mineral fertilizers application was investigated: N0P0K0, N60P60K60, N120P120K120 and N180P180K180. It was found that increasing the rate of fertilizer application increased the yield of dry raw thyme and the content of essential oil. The most effective rate of fertilizer application is N180P180K180, which provided a dry leaves yield of 2.48 t/ha with an essential oil content of 38.2 ml/ha.The results showed that with the increase of the number of plants per unit area, the share of leaves in the total mass of raw materials decreased from 52.3% to 45.1%, but due to a significant increase in plantation productivity, dry leaf yields increased. The highest productivity was recorded for the variant of cultivation schemes 45×10 cm with a planting density of 222 thousand plants/ha, where the yield of dried thyme leaves was 1.52 t/ha.


2017 ◽  
Vol 7 (4) ◽  
pp. 619-626 ◽  
Author(s):  
Mahsa Roodbaraky ◽  
Ali Mehrafarin ◽  
Farahnaz Khalighi-Sigaroodi ◽  
Hassanali Naghdi Badi

<p>Background &amp; aim: Bioactive compounds and plant growth regulators could change a plant’s essential oil content and composition. In this study, the effect of bioactive compounds and plant growth regulators on lippia citriodora was investigated.<br />Objective: This experiment was done with three replications on the basis of factorial experiment in randomized complete block design. The bio-regulators were sprayed in four levels, i.e. distilled water, 50 ppm gibberellic acid (GA3) + 50 ppm indole-3-butyric acid (IBA), 50 ppm GA3 + 100 ppm IBA, and 100 ppm GA3 + 50 ppm IBA. The chitosan was sprayed in two levels: distilled water and 400 ppm chitosan. The methanol was sprayed in two levels: distilled water and 5% v/v methanol. The essential oil was extracted by hydrodistillation method and analyzed by GC and GC/MS.<br />Results: Thirty-two compounds were identified, representing 95.08% of the total essential oil. The main components were E-citral (geranial) (25.02%), Z-citral (neral) (16.04%), ar-curcumene (10.37%), caryophyllene oxide (9.56%), spathulenol (6.83%), limonene (4.99%) and (E)-caryophyllene (2.29%). The studied traits were significantly affected (p &lt; 0.01) by the interaction of bioregulators, methanol and chitosan application.<br />Conclusion: Foliar application of IBA, GA3, chitosan and methanol could increase aromatic values of L. citriodora essential oil.</p>


2020 ◽  
Vol 24 (9) ◽  
pp. 909-1009
Author(s):  
Maryam Akaberi ◽  
Zahra Tayarani-Najaran ◽  
Iraj Mehregan ◽  
Javad Asili ◽  
Amirhossein Sahebkar ◽  
...  

One of the most important families of Iranian flora is Apiaceae (Umbelliferae). Most of the species of this family are aromatic plants and rich in essential oils with diverse structures. In the present review, the essential oil composition of 63 genera comprising 141 Apiaceae (66.4% native 33.6% endemic) is summarized.


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