scholarly journals GC/MS Analysis of Fatty Acids in Italian Dry Fermented Sausages

2015 ◽  
Vol 9 (1) ◽  
pp. 5-13 ◽  
Author(s):  
Angelo Liguori ◽  
Emilia Lucia Belsito ◽  
Maria Luisa Di Gioia ◽  
Antonella Leggio ◽  
Francesca Malagrinò ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Lipid Oxidation ◽  
Free Fatty Acids ◽  
Methyl Esters ◽  
Colorimetric Method ◽  
Monounsaturated Fatty Acids ◽  
Fatty Acids Profile ◽  
Fermented Sausages ◽  
Dry Fermented Sausages

The present investigation reports a study about the evolution, during ripening, of the fatty acid profile and the fatty acid composition in acylglycerols of three different fermented sausages industrially produced in the Calabria region (Southern Italy). Statistical analysis (ANOVA) was applied to the results obtained for the profiles to check all the differences between samples. The study comprised also an evaluation of the lipid oxidation level. All kind of sausages showed a free fatty acids profile in which the monounsaturated fatty acids were predominant, followed by saturated and polyunsaturated fatty acids. In acylglycerols, a low content in linoleic acid (approximately 2% of total methyl esters) was displayed, while that found in the free fatty acids profile was higher (approximately 17% of total FFAs). In addition, the generation of aldehydes through secondary lipid oxidation was clearly confirmed after long ripening period by classical qualitative colorimetric method supported by 1H NMR spectroscopy. The differences in fatty acids profiles observed in the free fatty acids and the acylglycerol fractions were certainly due to the high selectivity of lypase activity during the ripening.

scholarly journals Content and composition of lipid classes, fatty acid from \(\textit{Sargassum}\) seaweed collected at Con Dao and Van Phong bay

2021 ◽  
Vol 21 (3) ◽  
pp. 311-318
Author(s):  
Thu Hue Pham ◽  
Van Tuyen Anh Nguyen Nguyen ◽  
Yen Kieu Thi Hoang ◽  
Nguyen Nguyen ◽  
Hai Nam Hoang ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Free Fatty Acids ◽  
Total Lipid ◽  
Polar Lipid ◽  
Brown Seaweed ◽  
Monounsaturated Fatty Acids ◽  
Lipid Classes ◽  
Average Content ◽  
Fresh Weight

This study studied the content and composition of the total lipid, lipid classes and fatty acids in 13 brown seaweed Sargassum species collected from Con Dao and Van Phong, Vietnam. The total lipid has a low content and varies among species from 0.10–1.70% of the fresh weight. From 13 species, seven lipid classes including polar lipid (Pol), free fatty acids (FFA), sterol (ST), hydrocarbon and wax (HW), triacylglycerol (TG), diacylglycerol (DG), and monoalkydiacylglycerol (MADG). Using the GC-FID technique, we have identified 29 fatty acids classified into 3 groups of saturated fatty acid, monounsaturated fatty acids, polyunsaturated fatty acids with an average content of 44.93%, 24.57% and 27.44%, respectively. Among those, many value fatty acids have been detected with high content such as C18:3n-3, C20:4n-6, 20:5n-3, and 22:6n-3. The lipid of 13 brown seaweed Sargassum species also fully contains omega-3,6,9 fatty acids with the content of 9.28%, 16.28% and 16.63%, respectively.

scholarly journals Identification and Quantification of Fatty Acids in T. viridissima, C. biguttulus, and C. brunneus by GC-MS

2018 ◽  
Vol 2018 ◽  
pp. 1-8
Author(s):  
Alexander M. Wathne ◽  
Hanne Devle ◽  
Carl Fredrik Naess-Andresen ◽  
Dag Ekeberg
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Solid Phase Extraction ◽  
Free Fatty Acids ◽  
Dry Matter ◽  
Solid Phase ◽  
Neutral Lipids ◽  
Methyl Esters ◽  
Phase Extraction ◽  
Chorthippus Biguttulus

Fatty acid (FA) profiles of the species Tettigonia viridissima, Chorthippus biguttulus, and Chorthippus brunneus were determined and quantitated. Extracted lipids were derivatized into FA methyl esters (FAMEs) prior to analysis by GC-MS. A total of 37 different FAs were identified in T. viridissima, yielding a total FA content of 10.4 g/100 g of dry matter. The contents of saturated FAs, monounsaturated FAs, and polyunsaturated FAs were 31.1, 35.9, and 33.0%, respectively. Lipids from T. viridissima were also fractioned into neutral lipids, free fatty acids, and polar lipids by offline solid phase extraction. For C. brunneus and C. biguttulus, 33 FAs were identified, yielding a total FA content of 6.14 g/100 g of dry matter. SFAs, MUFAs, and PUFAs, respectively, constituted 32.7, 25.1, and 42.1% of the total FA content. The contents of MUFAs, PUFAs, n-3 FAs, and n-6 FAs of each species, and the n-6/n-3 ratio, were subsequently discussed.

Fatty Acid Profiles of Main Lipid Classes in Adult Chrysomela vigintipunctata (Scopoli) (Coleopterai:Chrysomelidae)

2000 ◽  
Vol 55 (7-8) ◽  
pp. 661-666 ◽  
Author(s):  
Nelly Nikolova ◽  
Tomas Rezanka ◽  
Boryana Nikolova-Damyanova
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Free Fatty Acids ◽  
Monounsaturated Fatty Acids ◽  
Carbon Chain ◽  
Leaf Beetle ◽  
Polar Lipids ◽  
Total Lipid Extract ◽  
Branched Chains ◽  
Willow Leaf Beetle

Abstract The fatty acid composition of the willow leaf beetle Chrysomela vigintipunctata (Scopoli) (Coleoptera: Chrysomelidae) is presented in this paper. Fatty acids in the total lipid extract, triacylglycerols, free fatty acids and polar lipids were compared. One hundred and fifteen fatty acids were identified in the total lipids. The mixture comprised compounds with normal and branched-chains of 12-30 carbon atoms and zero to six double bonds in different positions in the carbon chain. Substantial amounts of unsaturated eicosanoic fatty acids known as important precursor of eicosanoids in insects were detected in the lipids as were biologically significant positionally isomeric dienes, trienes and tetraenes of the series (n-3) and (n-6) of C16, C18, and C22 fatty acids. Also present was a mixture of hydroxy-FA. Triacylglycerols contained mostly saturated and monounsaturated fatty acids. Polyunsaturated fatty acids were found mostly in free fatty acids and especially in polar lipids.

Crude glycerine characterization: analysis of free fatty acids, fatty acid methyl esters, and acylglycerides

2020 ◽  
Author(s):  
Maira Alejandra Maquirriain ◽  
Lucas Gabriel Tonutti ◽  
Carlos Alberto Querini ◽  
María Laura Pisarello
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Free Fatty Acids ◽  
Fatty Acid Methyl Esters ◽  
Methyl Esters ◽  
Crude Glycerine ◽  
Acid Methyl

scholarly journals Analysis Fatty Acids Profile in Tabanus bivittatus Mats with Gas Chromatography-Mass Spectrometry

2015 ◽  
Vol 9 (1) ◽  
pp. 113-118
Author(s):  
Wang Yanhua ◽  
Wu Fuhua ◽  
Guo Zhaohan ◽  
Peng Mingxing ◽  
Xia Min ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Palmitoleic Acid ◽  
Unsaturated Fatty Acids ◽  
Methyl Esters ◽  
Soxhlet Extraction ◽  
Gas Chromatography Mass Spectrometry ◽  
Chemical Components ◽  
Fatty Acids Profile ◽  
Total Fatty Acids

Tabanus bivittatus Mats., a traditional Chinese medicine, is commonly used for cardiovascular disorders treatment including atherosclerosis. There have been only a few researches on its chemical components, and no detailed report has appeared on its fatty acids. To develop a simple and effective method for the extraction of total fatty acids from Tabanus bivittatus Mats., the Soxhlet extraction (SE) condition was optimized with response surface methodology. The fatty acid composition of the extract were determined by GC-MS with previous derivatization to fatty acid methyl esters (FAMEs). The major fatty acids in Tabanus bivittatus Mats. were oleic acid, palmitic acid, linoleic acid, palmitoleic acid, and stearic acid, and the unsaturated fatty acids occupy 63.9% of the total fatty acids.

scholarly journals Synthesis of Fatty Acid Methyl Esters from Jatropha curcas Oil and Its Purification Using Solvent Fractionation

2014 ◽  
Vol 13 (1) ◽  
pp. 31
Author(s):  
Avita Kusuma Wardhani ◽  
Chusnul Hidayat
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Free Fatty Acids ◽  
Jatropha Curcas ◽  
Fatty Acid Methyl Esters ◽  
Methyl Esters ◽  
Solvent Fractionation ◽  
Jatropha Curcas Oil ◽  
Acid Methyl ◽  
Purification Factor

Fatty acid methyl esters (FAME) are produced by transesterification. The problem in the product of transesterification is the presence of impurities such as mono-, di-, triglycerides, and free fatty acids. So that, the purification using solvent fractionation is needed to separate them from FAME. The objective of this research were to determine the effects of crude fatty acid methyl esters-to-acetone (CFAME/acetone) ratio on yield, purity, purification factor, and recovery of FAME after fractionation and to evaluate the impurities which were separated in each step of fractionation. FAME were produced from Jatropha curcas oil using Berchmans’s and Tiwari’s methods. The impurities were separated by solvent fractionation using acetone. CFAME/acetone ratios were 1, 2, 3, 4, and 5. Fractionation was done stepwise namely 21°C, 16°C, 12°C, and 5°C. The results showed that the conversion of FAME using Tiwari’s method was 1.7-fold higher than Berchmans’s method. Purification of FAME using solvent fractionation resulted that the best CFAME/acetone ratio was 1. Yield decreased 1.6-fold at CFAME/acetone ratio 4. Purity decreased 8.74% with an increase in CFAME/acetone ratio 1 to 5. Purification factor decreased 2-fold at CFAME/acetone 1 to 3. Recovery decreased 1.3-fold at CFAME/acetone ratio 1 to 4. The impurities which were separated from FAME were mono-, di-, triglycerides, and free fatty acids and the major component of impurities was triglycerides (>59%). The results indicated that solvent fractionation could be used as an alternative method for purifying FAME and further study to optimize this method was needed.

scholarly journals Role of free fatty acids and insulin in determining free fatty acid and lipid oxidation in man.

1991 ◽  
Vol 87 (1) ◽  
pp. 83-89 ◽  
Author(s):  
L C Groop ◽  
R C Bonadonna ◽  
M Shank ◽  
A S Petrides ◽  
R A DeFronzo
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Lipid Oxidation ◽  
Free Fatty Acids

scholarly journals Adding Blends of NaCl, KCl, and CaCl2to Low-Sodium Dry Fermented Sausages: Effects on Lipid Oxidation on Curing Process and Shelf Life

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Bibiana Alves dos Santos ◽  
Paulo Cezar Bastianello Campagnol ◽  
Mariane Bittencourt Fagundes ◽  
Roger Wagner ◽  
Marise Aparecida Rodrigues Pollonio
Keyword(s):  
Fatty Acids ◽  
Lipid Oxidation ◽  
Meat Products ◽  
Good Alternative ◽  
Curing Process ◽  
Low Sodium ◽  
Oxidative Reactions ◽  
Fermented Sausages ◽  
Dry Fermented Sausages ◽  
And Storage

The effect of a 50% reduction of NaCl and its replacement by KCl, CaCl2, and a blend of KCl and CaCl2(1 : 1) on lipid oxidation of dry fermented sausages was investigated. We found that a 50% reduction in NaCl decreased the intensity of the reactions to lipid oxidation, while treatments with added CaCl2resulted in increased lipid oxidation during manufacture and storage. Fatty acid composition also changed owing to the presence of KCl and CaCl2, showing a decrease in saturated, monounsaturated, and polyunsaturated fatty acids after 30 days of storage. Furthermore, a decreased intensity ofL⁎and increasedb⁎values were found in salamis with CaCl2. These results suggest that using CaCl2as a substitute for NaCl increases the intensity of oxidative reactions while the addition of KCl could be a good alternative to reduce the NaCl content in fermented meat products.

Effect of feeding diets high in monounsaturated fatty acids and α-tocopheryl acetate to rabbits on resulting carcass fatty acid profile and lipid oxidation

1997 ◽  
Vol 64 (1) ◽  
pp. 177-186 ◽  
Author(s):  
C. Lopez-Bote ◽  
A. Rey ◽  
J. Ruiz ◽  
B. Isabel ◽  
R. Sanz Arias
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Lipid Oxidation ◽  
Relative Proportion ◽  
Monounsaturated Fatty Acids ◽  
Membrane Lipid ◽  
Tocopheryl Acetate ◽  
Gas Liquid Chromatography ◽  
Animal Tissues ◽  
Volatile Aldehydes

AbstractThe effects of olive (MONO) or sunflower (POLY) oil-enriched (30 g/kg) diets with either a basal (10 mg/kg food) or supplemented (200 mg/kg) level of a-tocopheryl acetate on some measures of production, fatty acid composition of animal tissues and susceptibility to oxidation of rabbit meat and membrane extracts have been studied. MONO diet produced higher levels of C18:1 in animal tissues. Animals that received POLY diet had a higher level of C18:2 in perirenal and neutral fraction of intramuscular fat and higher levels of C18:2, C20:4, C22:4 and C22:5 in phospholipid, reaching a higher overall unsaturation (P = 0·001). Muscle samples from rabbits given the POLY diet were more susceptible to lipid oxidation (P = 0·0001). Differences in membrane lipid oxidation, between groups followed a similar pattern to that of meat. Diets rich in C18:2 resulted in increases in concentration of pentanal (P < 0·001), hexanal (P = 0·0001) and total volatile aldehydes (P = 0·0001) in meat as monitored by headspace gas liquid chromatography. Dietary supplementation with α-tocopheryl acetate reduced the overall concentration of volatile aldehydes (P < 0·05), particularly hexanal (P < 0·05). Dietary administration of monounsaturated fatty acids not only reduces membrane and meat lipid oxidation but also modifies the relative proportion of volatile aldehydes generated upon heating, with a specific decrease in those generally related to rancidity and off-flavour of meats.

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