Effect of Boswellia serrata on Rat Trachea Contractility In Vitro

2020 ◽  
Vol 10 (1) ◽  
pp. 33-43
Author(s):  
Wafaa Ahmed Hewedy
Keyword(s):  
Taste Receptor ◽  
Inhibitory Effect ◽  
Boswellia Serrata ◽  
Traditional Use ◽  
Relaxant Effect ◽  
Airway Diseases ◽  
Bowel Diseases ◽  
Male Wistar Rats ◽  
Rat Trachea

Background: Boswellia serrata (family Burseraceae) has been traditionally used for the treatment of a wide variety of diseases as arthritis, inflammatory bowel diseases, and airway diseases. However, the direct bronchodilator efficacy of Boswellia serrata hasn’t been explored yet. Objective: We aimed at the present study to evaluate the direct effect of Boswellia serrata extract (BSE) on isolated rat tracheal preparations precontracted with either Acetylcholine (ACh) or potassium chloride (KCl). Methods: Tracheal rings were prepared from male Wistar rats (200-250 g). BSE (1-200 μg/ml) was added to tracheal strips precontracted with either ACh or KCl and the response was observed. We also investigated the consequences of epithelial denudation, indomethacin, and N-Nitro-L-arginine on the relaxant effect of BSE as compared to that of the β-adrenoceptor agonist isoprenaline, or the bitter taste receptor (TAS2R) agonist denatonium benzoate. Finally, the possible additive effects of BSE to isoprenaline or denatonium-induced relaxation were evaluated. Results: By using a set of serial dosing and washout experiments with tracheal rings, results showed that exposure to BSE resulted into a significant and concentration-dependent inhibitory effect on airway smooth muscle contractions precontracted with either ACh or KCl. Epithelial denudation, indomethacin, or N-Nitro-L-arginine had no significant effect on the obtained relaxation. Furthermore, BSE potentiated the relaxant effect of isoprenaline on rat trachea. Conclusion: BSE exerts a direct concentration-dependent relaxant effect on precontracted tracheal strips. These results could contribute towards validation of the traditional use of BSE in the treatment of airway diseases.

scholarly journals Pharmacological Evaluation of the Bronchorelaxant Effect of Waltheria indica L. (Malvaceae) Extracts on Rat Trachea

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Rainatou Boly ◽  
Zakaline Yabre ◽  
Mathieu Nitiema ◽  
Boubacar Yaro ◽  
Jules Yoda ◽  
...  
Keyword(s):  
Relaxation Effect ◽  
Phytochemical Screening ◽  
Oral Toxicity ◽  
Traditional Use ◽  
Hydroalcoholic Extract ◽  
Relaxant Effect ◽  
K Channels ◽  
Rat Trachea ◽  
Isolated Rat

Waltheria indica L. (Malvaceae) is a plant used in Burkina Faso for the treatment of various ailments including asthma. The aim of the study was to evaluate the pharmacological relaxant effect of the leafy stem extracts of Waltheria indica and thereby verify claim of use in treating asthma. Aqueous decoction and hydroalcoholic extracts obtained from the powdered leafy stems were screened for the presence of some phytoconstituents. The in vitro relaxant effect of the two extracts was evaluated on acetylcholine- (ACh 10−5 M) and potassium chloride- (KCl 6 × 10−2 M) induced contractions on rat-isolated tracheal preparations. To examine whether the potassium (K+) channels are involved in the relaxant effect, glibenclamide, an ATP-sensitive potassium channel inhibitor, was used. Moreover, to assess the safety of the extracts, acute oral toxicity was carried out on mice. The phytochemical screening revealed the presence of alkaloids, flavonoids, saponins, steroids, triterpenoids, tannins, and coumarins in the hydroalcoholic extract. Tannins, steroids, triterpenoids, and coumarins were not detected in the aqueous decoction. With respective EC50 values of 1.517 ± 0.002 mg/mL and 1.433 ± 0.001 mg/mL on ACh-and KCl-provoked contractions, the hydroalcoholic extract was found more potent in relaxing the isolated rat tracheal preparations compared to the aqueous decoction. In the presence of glibenclamide, the relaxant effect of the hydroalcoholic extract (EC50 = 0.191 ± 0.002 mg/mL) increased and was higher than that of the aqueous decoction. At dose of 5000 mg/kg of body weight, the extracts did not produce deaths or any significant changes in the general behavior of mice. The results suggest that different mechanisms including modulation of calcium and potassium channels, particularly the ATP-sensitive K+ channels, could be involved in the relaxation effect. These findings could justify the traditional use of W. indica in the management of asthma.

scholarly journals Protective effects of Trigonella foenum-graecum crude extract over damage induced by Sreptozotocin diabetes rats

2020 ◽  
Vol 10 (4-s) ◽  
pp. 138-141
Author(s):  
Dalila Bencheikh ◽  
Seddik Khennouf ◽  
Saliha Dahamna
Keyword(s):  
Streptozotocin Diabetes ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Protective Effects ◽  
Traditional Use ◽  
Orogastric Tube ◽  
Trigonella Foenum Graecum ◽  
Male Wistar Rats ◽  
Treatment Of Diabetes

The seeds of Trigonella foenum-graecum (fenugreek) are used for treatment of diabetes mellitus in traditional medicine. This paper examines the protection effects of fenugreek from the damage induced by streptozotocin diabetes rats. Tannins content of T. foenum-graecum was also estimated in vitro. Normoglycemic male Wistar rats, weighing 170-250 g, were selected and randomly divided into five groups (n= 6): normal control, diabetic + TFGE (200mg/kg), diabetic+ TFGE (600mg/kg), diabetic + Glibil (3mg/kg), untreated group. Diabetes was induced after a single intraperitoneal injection of streptozotocin (50 mg/kg body weight) and Fenugreek was given every day via orogastric tube for 18 days. At the end of experiment, rats were sacarificed. Organ weight was estimated of all groups. Trigonella foenum-graecum administration significantly improved the polydipsia, polyphagia, and it also compensated weight loss of diabetic rats (P<0.05, P < 0.01). Moreover, fenugreek had a significant concentration of tannins (806.22 ±0.036 µg TAE/gE). The results revealed that fenugreek improves the damage in diabetic rats that in some ways validates the traditional use of this plant in treatment of diabetes. Keywords: Antidiabetic activity, Protective effect, Streptozotocin, Tannins, Trigonella foenum-graecum

scholarly journals INHIBITORY EFFECT OF ETHANOLIC EXTRACT OF ERIOBOTRYA JAPONICA LEAVES PRE-INCUBATED WITH THEOPHYLLINE AND ASPIRIN ON ISOLATED GUINEA PIG TRACHEAL

2018 ◽  
Vol 11 (13) ◽  
pp. 46
Author(s):  
Marianne Marianne ◽  
Urip Harahap ◽  
Emil Salim ◽  
Dadang Irfan Husori ◽  
Fahrumsyah Jali Rambe ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Protective Effect ◽  
Contractile Response ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Inhibitory Effect ◽  
Eriobotrya Japonica ◽  
Relaxant Effect ◽  
Significant Difference

 Objectives: The objectives of the study were to examine the inhibitory effect of ethanol extract of Eriobotrya japonica leaves (EEEJL) pre-incubated with theophylline and aspirin on isolated guinea pig tracheal chains against acetylcholine (ACh)-induced contraction.Methods: The effect of EEEJL (1-8 mg/Ml) on the isolated tracheal strips was tested in vitro. Furthermore, the mechanism of relaxant effects of EEEJL was evaluated in the presence of theophylline and aspirin.Results: The contractile response evoked by Ach (1.25 × 10−3 M) was decreased by EEEJL (effective concentration50 = 1.36 mg/mL) and has no significant difference of relaxant effect to that of EEEJL pre-incubated with theophylline and aspirin (p>0.05).Conclusion: The EEEJL decreased the ACh-induced contraction through the inhibition of PDE and the protective effect on prostaglandin E2.

scholarly journals Lack of Small Intestinal Dysbiosis Following Long-Term Selective Inhibition of Cyclooxygenase-2 by Rofecoxib in the Rat

Cells ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 251 ◽  
Author(s):  
Bernadette Lázár ◽  
Gábor Brenner ◽  
András Makkos ◽  
Mihály Balogh ◽  
Szilvia László ◽  
...  
Keyword(s):  
Selective Inhibition ◽  
Mucosal Damage ◽  
Inhibitory Effect ◽  
Intestinal Dysbiosis ◽  
Bowel Diseases ◽  
Direct Inhibitory Effect ◽  
Cox 2 ◽  
Small Intestinal

Intestinal dysbiosis is linked to numerous gastrointestinal disorders, including inflammatory bowel diseases. It is a question of debate if coxibs, selective inhibitors of cyclooxygenase (COX)-2, cause dysbiosis. Therefore, in the present study, we aimed to determine the effect of long-term (four weeks) selective inhibition of COX-2 on the small intestinal microbiota in the rat. In order to avoid mucosal damage due to topical effects and inflammation-driven microbial alterations, rofecoxib, a nonacidic compound, was used. The direct inhibitory effect of rofecoxib on the growth of bacteria was ruled out in vitro. The mucosa-sparing effect of rofecoxib was confirmed by macroscopic and histological analysis, as well as by measuring the intestinal levels of cytokines and tight junction proteins. Deep sequencing of bacterial 16S rRNA revealed that chronic rofecoxib treatment had no significant influence on the composition and diversity of jejunal microbiota. In conclusion, this is the first demonstration that long-term selective inhibition of COX-2 by rofecoxib does not cause small intestinal dysbiosis in rats. Moreover, inhibition of COX-2 activity is not likely to be responsible per se for microbial alterations caused by some coxibs, but other drug-specific properties may contribute to it.

scholarly journals Bronchodilatory effect of Myxopyrum serratulum in animal model

2017 ◽  
Vol 12 (1) ◽  
pp. 84
Author(s):  
Vijayalakshmi Maruthamuthu ◽  
Ruckmani Kandasamy
Keyword(s):  
Guinea Pig ◽  
Methanolic Extract ◽  
Relaxation Effect ◽  
Inhibitory Effect ◽  
Chlorpheniramine Maleate ◽  
Relaxant Effect ◽  
Significant Inhibitory Effect ◽  
Guinea Pig Model

<p class="Abstract">The plant <em>Myxopyrum </em>serratulum is traditionally claimed to relieve asthma and cough. The present study was undertaken to evaluate the bronchodilatory effect of the methanolic extract of <em>M. </em>serratulum on histamine-induced bronchospasm by <em>in vivo</em> and the inhibitory effect of the extract on histamine-contracted tracheal chain and ileum by <em>in vitro</em> guinea pig model. Additionally, the relaxant effect of four cumulative concentrations of the extract (0.25, 0.5, 0.7 and 1.0 g%) was assessed using precontracted tracheal chain under different conditions. The extract (400 mg/kg) prolonged the preconvulsive time to 102.3 ± 3.8 sec when compared to saline and standard chlorpheniramine maleate as 121.3 ± 4.5 sec (p&lt;0.05). The extract also possessed significant inhibitory effect on histamine-contracted guinea pig ileum and tracheal chain and also exhibited significant relaxation effect on precontracted tracheal chain of guinea pig models contracted by 60 mM KCl (p&lt;0.001) and 10 µM methacholine (p&lt;0.001) when compared with standard theophylline.</p>

scholarly journals Vasorelaxation in rat pulmonary artery induced by the monoterpene thymol: evaluation of the endothelium derived relaxant factors dependence

2021 ◽  
Vol 10 (4) ◽  
pp. e29010413971
Author(s):  
Renata de Souza Sampaio ◽  
Giuliana Amanda de Oliveira ◽  
Luiz Henrique César Vasconcelos ◽  
Paula Benvindo Ferreira ◽  
Maria da Conceição Correia Silva ◽  
...  
Keyword(s):  
Essential Oil ◽  
Pulmonary Artery ◽  
Inhibitory Effect ◽  
Tonic Contraction ◽  
Relaxant Effect ◽  
Vasorelaxant Effect ◽  
Cholinergic Pathway

Thymol and carvacrol are the main compounds found in Lippia mycrophylla essential oil (LM-OE) and have presented some spasmolytic effects. This work was designed to explore a possible vasorelaxant effect of LM-OE and its major monoterpenes constituents on rat pulmonary artery. For that, the organ was in vitro stimulated with phenylephrine (Phe) 3 mM and over the tonic contraction the relaxant effect of LM-OE, carvacrol and thymol was observed in both intact and denuded-endothelium. Moreover, atropine, L-NAME, indomethacin, 2,3-O-isopropylidene adenosine, H-89 and Y-27632 were incubated before the relaxant curve of thymol over Phe-tonic contraction. Furthermore, the effects of thymol on KCl 30 or 80 mM and S-(−)-Bay K8644-induced tonic contractions were evaluated, as well as its inhibitory effect on CaCl2-induced cumulative contractions. LM-OE, carvacrol and thymol presented relaxant effect on pulmonary artery, thymol was the most potent and its relaxant potency in intact-endothelium preparations was reduced by atropine, L-NAME, indomethacin, 2,3-O-isopropylidene adenosine and H-89, despite there was not change on its maximum relaxat effect. Also, the monoterpene relaxed equipotently KCl 30 or 80 mM pre-contracted pulmonary artery, antagonized CaCl2-induced cumulative contractions and relaxed S-(−)-Bay K8644 pre-contracted organ. Ultimately, thymol relaxant potency was not modified by Y-27632. Therefore, thymol acts by endothelium-dependent and independent mechanisms, possibly positively modulating the endothelial cholinergic pathway, prostanoids release and further activation of AC/PKA and also inhibiting Ca2+ influx through CaV.

scholarly journals Effects of Boswellia serrata Roxb. and Curcuma longa L. in an In Vitro Intestinal Inflammation Model Using Immune Cells and Caco-2

2018 ◽  
Author(s):  
Paolo Governa ◽  
Maddalena Marchi ◽  
Veronica Cocetta ◽  
Bianca De Leo ◽  
Philippa T. K. Saunders ◽  
...  
Keyword(s):  
Mechanism Of Action ◽  
Immune Cells ◽  
Inflammatory Bowel Diseases ◽  
Intestinal Inflammation ◽  
Curcuma Longa ◽  
Intestinal Barrier Function ◽  
Boswellia Serrata ◽  
Bowel Diseases ◽  
Inflammatory Bowel

Inflammatory bowel diseases, which consist of chronic inflammatory conditions of the colon and the small intestine, are considered a global disease of our modern society. Recently, the use of herbal therapies has increased in patients with inflammatory bowel diseases because of their effectiveness and better safety profile, compared to conventional drugs. Boswellia serrata Roxb. and Curcuma longa L. are amongst the most promising herbal drugs, however, their clinical use in inflammatory bowel diseases is limited and little is known on their mechanism of action. The aim of this work was to investigate the effects of two standardized extract of B. serrata and C. longa in an in vitro model of intestinal inflammation. Their impact on cytokine release and reactive oxygen species production, as well as the maintenance of the intestinal barrier function and on intestinal mucosa immune cells infiltration, has been evaluated. The extracts showed a good protective effect on the intestinal epithelium at 1 &micro;g/ml, with C. longa having an anti-inflammatory mechanism of action and B. serrata acting as an antioxidant. In summary, these herbal products were demonstrated to be promising agents for the management of inflammatory bowel diseases by modulating in vitro parameters which have been identified in the clinical conditions.

scholarly journals α-Glucosidase Inhibitory and Antiradical Properties of Acacia macrostachya

2020 ◽  
Vol 2 (5) ◽  
Author(s):  
Hamidou Têeda Ganamé ◽  
Yssouf Karanga ◽  
Ousmane Ilboudo ◽  
Wende-Konté Hazael Conania Nikiema ◽  
Richard Wamtinga Sawadogo ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Antiradical Activity ◽  
Methanolic Extract ◽  
Inhibitory Effect ◽  
Traditional Use ◽  
Methanol Extracts ◽  
Antiradical Properties ◽  
Ic50 Values ◽  
Vitro Model

In this work, the anti-diabetic activity of three extracts of Acacia macrostachya was investigated by following the inhibitory effect of these extracts on -glucosidase using the in vitro model. The antiradical activity of these extracts was also determined. Methanol extracts of root and stem barks showed a very significant inhibitory effect against the enzyme activity of -glucosidase with IC50 2.487 ± 0.441 µg/mL and 1.650 ± 0.229 µg/mL respectively. For antiradical activity, the same extracts presented the highest scavenging of the radical DPPH● with IC50 values of 9.307 ± 0.262 µg/mL and 5.242 ± 0.068 µg/mL respectively. With the cationic radical ABTS●+, IC50 varied from 45.049 ± 0.730 µg/mL for methanolic root barks extract to 14.136 ± 0.161 µg/mL for methanolic extract from stem barks. Thus, the methanol extracts of the root and stem barks of Acacia macrostachya possess compounds with very interesting anti-diabetic and antiradical properties and could justify its traditional use.

scholarly journals Effect of Hydro Alcoholic Extracts of Boswellia serrata and Terminalia bellerica Against Cyclo-Oxygenase and Lipoxygenase Enzymes- An In Vitro Approach

2017 ◽  
Vol 9 (08) ◽  
Author(s):  
Rajesh S. Mony ◽  
Sreenath Kunnathupura Baskaran ◽  
Muneeswari Perumalsamy ◽  
Poornima Kannappan
Keyword(s):  
Inhibitory Effect ◽  
Boswellia Serrata ◽  
Hydroalcoholic Extract ◽  
Terminalia Bellerica ◽  
Standard Procedures ◽  
Maximum Inhibition ◽  
Physiological Abnormalities ◽  
Cox 2 ◽  
Inhibitory Potential

Introduction: Inflammation is a key mediator in plenty of important physiological abnormalities like cancer, myocardial infarction and arthritis. Natural anti-inflammatory agents are of great demand owing to its decreased side effects and increased curative properties. Aim: To investigate the in vitro inhibitory effect of three different concentrations of hydroalcoholic extract of Boswellia serrata (BS) and Terminalia bellerica (TB) on COX-2 and LOX-2 enzymes. Materials and Methods: Three different concentrations of hydroalcoholic menstruum of BS and TB were prepared using the standard procedures. The in vitro inhibitory activities of COX-2 and LOX-2 were performed using previously mentioned methods with minor modifications. Results: Among the different concentrations used the 90:10 menstruum of BS at 12 mg/ml displayed maximum inhibitory potential against COX-2 enzyme when compared to the same concentration of TB extract. This is suggestive that the majority of bioactive compounds present in BS was alcohol soluble and hence elicited maximum inhibition potential at 90 % ethanol ratio. A similar pattern of results were observed against LOX-2 enzyme as well. Conclusion:From the results it is concluded that the 90:10 hydroalcoholic extract of Boswellia serrata and Terminalia bellerica exhibit appreciable percentage of COX-2 and LOX-2 inhibition, which could be attributed to the ethanol soluble bioactive components present in the extract.

scholarly journals Effects of Boswellia Serrata Roxb. and Curcuma longa L. in an In Vitro Intestinal Inflammation Model Using Immune Cells and Caco-2

2018 ◽  
Vol 11 (4) ◽  
pp. 126 ◽  
Author(s):  
Paolo Governa ◽  
Maddalena Marchi ◽  
Veronica Cocetta ◽  
Bianca De Leo ◽  
Philippa Saunders ◽  
...  
Keyword(s):  
Mechanism Of Action ◽  
Immune Cells ◽  
Inflammatory Bowel Diseases ◽  
Intestinal Inflammation ◽  
Curcuma Longa ◽  
Intestinal Barrier Function ◽  
Boswellia Serrata ◽  
Bowel Diseases ◽  
Inflammatory Bowel

Inflammatory bowel diseases, which consist of chronic inflammatory conditions of the colon and the small intestine, are considered a global disease of our modern society. Recently, the interest toward the use of herbal therapies for the management of inflammatory bowel diseases has increased because of their effectiveness and favourable safety profile, compared to conventional drugs. Boswellia serrata Roxb. and Curcuma longa L. are amongst the most promising herbal drugs, however, their clinical use in inflammatory bowel diseases is limited and little is known on their mechanism of action. The aim of this work was to investigate the effects of two phytochemically characterized extracts of B. serrata and C. longa in an in vitro model of intestinal inflammation. Their impact on cytokine release and reactive oxygen species production, as well as the maintenance of the intestinal barrier function and on intestinal mucosa immune cells infiltration, has been evaluated. The extracts showed a good protective effect on the intestinal epithelium at 1 µg/mL, with TEER values increasing by approximately 1.5 fold, compared to LPS-stimulated cells. C. longa showed an anti-inflammatory mechanism of action, reducing IL-8, TNF-α and IL-6 production by approximately 30%, 25% and 40%, respectively, compared to the inflammatory stimuli. B. serrata action was linked to its antioxidant effect, with ROS production being reduced by 25%, compared to H2O2-stimulated Caco-2 cells. C. longa and B. serrata resulted to be promising agents for the management of inflammatory bowel diseases by modulating in vitro parameters which have been identified in the clinical conditions.

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