Effectively utilizing Peracetic Acid (PAA) to achieve the State of Missouri's E-coli permit requirements of 206 cfu/100 ml for wastewater disinfection

At the pilot-scale, peracetic acid effectively inactivates fecal coliforms,E. coliandEnterococcusspp. in secondary and tertiary treated wastewater. The addition of UV to PAA treatment increases inactivation of somatic coliphage.

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Water and Wastewater Disinfection with Peracetic Acid and UV Radiation and Using Advanced Oxidative Process PAA/UV

International Journal of Photoenergy ◽

10.1155/2015/860845 ◽

2015 ◽

Vol 2015 ◽

pp. 1-7 ◽

Cited By ~ 24

Author(s):

Jeanette Beber de Souza ◽

Fernanda Queiroz Valdez ◽

Rhuan Felipe Jeranoski ◽

Carlos Magno de Sousa Vidal ◽

Grasiele Soares Cavallini

Keyword(s):

Uv Radiation ◽

Peracetic Acid ◽

Combined Method ◽

Experimental Conditions ◽

E Coli ◽

Wastewater Disinfection ◽

Combined Action ◽

Oxidative Processes ◽

Water And Wastewater ◽

The Individual

The individual methods of disinfection peracetic acid (PAA) and UV radiation and combined process PAA/UV in water (synthetic) and sanitary wastewater were employed to verify the individual and combined action of these advanced oxidative processes on the effectiveness of inactivation of microorganisms indicators of fecal contaminationE. coli, total coliforms (in the case of sanitary wastewater), and coliphages (such as virus indicators). Under the experimental conditions investigated, doses of 2, 3, and 4 mg/L of PAA and contact time of 10 minutes and 60 and 90 s exposure to UV radiation, the results indicated that the combined method PAA/UV provided superior efficacy when compared to individual methods of disinfection.

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Urban wastewater disinfection for agricultural reuse: effect of solar driven AOPs in the inactivation of a multidrug resistant E. coli strain

Applied Catalysis B Environmental ◽

10.1016/j.apcatb.2014.10.043 ◽

2015 ◽

Vol 178 ◽

pp. 65-73 ◽

Cited By ~ 73

Author(s):

Giovanna Ferro ◽

Antonino Fiorentino ◽

María Castro Alferez ◽

M. Inmaculada Polo-López ◽

Luigi Rizzo ◽

...

Keyword(s):

Multidrug Resistant ◽

Coli Strain ◽

Urban Wastewater ◽

E Coli ◽

Wastewater Disinfection

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Advanced wastewater disinfection technologies: short and long term efficiency

Water Science & Technology ◽

10.2166/wst.1998.0516 ◽

1998 ◽

Vol 38 (12) ◽

pp. 109-117 ◽

Cited By ~ 39

Author(s):

V. Lazarova ◽

M. L. Janex ◽

L. Fiksdal ◽

C. Oberg ◽

I. Barcina ◽

...

Keyword(s):

Peracetic Acid ◽

Contact Time ◽

Pilot Scale ◽

Coli Strain ◽

Plate Count ◽

Galactosidase Activity ◽

Virus Removal ◽

E Coli ◽

Bacteriophage Ms2 ◽

Short And Long Term

Advanced disinfection processes (peracetic acid, UV irradiation and ozonation) have been tested and evaluated through bench and pilot scale studies. 3 log removals of total coliforms, faecal coliforms and faecal streptococci were achieved by 10mg/L peracetic acid at a 10min contact time, by UV radiation at 35mW.s/cm2 and by ozone at 5mg/L for 10min contact time. Higher doses are required for virus removal by UV and PAA and especially for highly resistant viruses such as F-specific bacteriophage MS2. Ozonation has the advantage of having a strong effect on all types of bacteriophages and protozoa cysts even when low treatment doses and short contact times are applied. The results of this study demonstrated that evaluation of disinfection efficiency of ozone, UV and PAA depends on the criteria and methods employed. Standard method (plate count) results showed an important disinfection effect on culturability, while results from non-standard methods (respiratory activity and β-galactosidase activity assay) indicated less reduction of viable cells. Moreover, the results confirm that disinfectants act on bacteria in different ways. It has been clearly demonstrated that b-galactosidase activity is affected by PAA while UV treatment has no or very limited effect on the enzyme activity. Even without sunlight reactivation, bacterial regrowth in seawater was observed after disinfection of sewage effluents. This study also shows that the biodegradability of sewage effluent for an E coli strain was affected differently by the oxidative disinfectants ozone and PAA. Biodegradability should therefore be considered when evaluating the total disinfection efficiency.

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A Clean-In-Place Type Sanitation Method Validation for a Benchtop Meat Grinder

Meat and Muscle Biology ◽

10.22175/mmb.10732 ◽

2019 ◽

Vol 3 (2) ◽

Author(s):

C. E. Rayfield ◽

R. Jadeja ◽

S. Billups

Keyword(s):

Lactic Acid ◽

Foodborne Pathogens ◽

Peracetic Acid ◽

Deionized Water ◽

Cross Contamination ◽

Direct Plating ◽

Water Ice ◽

E Coli ◽

Different Types ◽

Low Levels

ObjectivesThis research is designed to validate a novel clean-in-place type antimicrobial ice-based meat grinder sanitation method.Materials and MethodsFour different types of antimicrobial ice were prepared from peracetic acid (PAA, 350 mg/L) and combination PAA with 2% FreshFX® (PAAF), 2% Paradigm® (PAAP) and 2% lactic acid (PAAL). The grinders were inoculated by processing 400 g beef trim containing 400 μL of E. coli O157:H7 or S. Typhimurium DT 104 suspensions at 8.4 to 8.7 (high inoculation) and 5.3 to 5.5 (low inoculation) log CFU/mL. Each meat grinder was then treated by processing 1000 g of antimicrobial ice and 500 mL of corresponding antimicrobial solution. At the end of each treatment, 400 g un-inoculated beef was processed through the meat grinder, and the resulting ground beef was then analyzed for the presence of target pathogens by direct plating and after enrichment. Efficacies of antimicrobial ice-based treatments were compared with 1000 g deionized water ice + 500 mL deionized water (DI), and no treatment (NT) controls.ResultsAll antimicrobial ice treatments were able to reduce cross-contamination to non-detectable levels from the meat grinders inoculated at the low levels of pathogens, but after enrichment, target pathogens were detected in all the samples. Recoveries from the meat grinder inoculated with high levels of pathogens ranged from 5.95 to 3.50 log CFU/g and 5.86 to 3.46 log CFU/g for E. coli O157:H7 and S. Typhimurium DT 104, respectively. All antimicrobial ice treatments were significantly (p ≤ 0.05) more effective in reducing cross-contamination in comparison of NT and DI controls. The microbial reductions achieved by different antimicrobial ice treatments were not significantly (p ≤ 0.05) different from each other.ConclusionThe antimicrobial ice-based meat grinder sanitation technique could effectively reduce foodborne pathogens from meat grinders without needing meat grinder disassembly.

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Impact of UV and Peracetic Acid Disinfection on the Prevalence of Virulence and Antimicrobial Resistance Genes in Uropathogenic Escherichia coli in Wastewater Effluents

Applied and Environmental Microbiology ◽

10.1128/aem.00418-14 ◽

2014 ◽

Vol 80 (12) ◽

pp. 3656-3666 ◽

Cited By ~ 24

Author(s):

Basanta Kumar Biswal ◽

Ramzi Khairallah ◽

Kareem Bibi ◽

Alberto Mazza ◽

Ronald Gehr ◽

...

Keyword(s):

Escherichia Coli ◽

Antimicrobial Resistance ◽

Resistance Genes ◽

Peracetic Acid ◽

Content Type ◽

E Coli ◽

Antimicrobial Resistance Genes ◽

Antimicrobial Resistance Gene ◽

Municipal Wastewaters ◽

The Impact

ABSTRACTWastewater discharges may increase the populations of pathogens, includingEscherichia coli, and of antimicrobial-resistant strains in receiving waters. This study investigated the impact of UV and peracetic acid (PAA) disinfection on the prevalence of virulence and antimicrobial resistance genes in uropathogenicEscherichia coli(UPEC), the most abundantE. colipathotype in municipal wastewaters. Laboratory disinfection experiments were conducted on wastewater treated by physicochemical, activated sludge, or biofiltration processes; 1,766E. coliisolates were obtained for the evaluation. The target disinfection level was 200 CFU/100 ml, resulting in UV and PAA doses of 7 to 30 mJ/cm2and 0.9 to 2.0 mg/liter, respectively. The proportions of UPECs were reduced in all samples after disinfection, with an average reduction by UV of 55% (range, 22% to 80%) and by PAA of 52% (range, 11% to 100%). Analysis of urovirulence genes revealed that the decline in the UPEC populations was not associated with any particular virulence factor. A positive association was found between the occurrence of urovirulence and antimicrobial resistance genes (ARGs). However, the changes in the prevalence of ARGs in potential UPECs were different following disinfection, i.e., UV appears to have had no effect, while PAA significantly reduced the ARG levels. Thus, this study showed that both UV and PAA disinfections reduced the proportion of UPECs and that PAA disinfection also reduced the proportion of antimicrobial resistance gene-carrying UPEC pathotypes in municipal wastewaters.

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The state of humoral antibacterial immunity in purulent-inflammatory diseases of the abdominal cavity

Kazan medical journal ◽

10.17816/kazmj71988 ◽

2021 ◽

Vol 82 (1) ◽

pp. 42-44

Author(s):

V. F. Chikaev ◽

N. A. Safina ◽

O. D. Zinkevich ◽

O. V. Gevorkyan

Keyword(s):

Enzyme Immunoassay ◽

Inflammatory Diseases ◽

Abdominal Cavity ◽

The State ◽

E Coli

We have studied the level of antibacterial antibodies to E coli. Rg. mirabilis, Ps. aeruginosa, Bact. fragilis, St. aureus, as well as to the endotoxin of grammatical bacteria (glycolipid - HLP) by enzyme immunoassay in plasma in patients with varying degrees of prevalence of the pyoinflammatory process in the abdominal cavity.

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Pilot-plant comparative study of peracetic acid and sodium hypochlorite wastewater disinfection

Water Research ◽

10.1016/s0043-1354(02)00248-8 ◽

2003 ◽

Vol 37 (1) ◽

pp. 78-94 ◽

Cited By ~ 55

Author(s):

E Veschetti ◽

D Cutilli ◽

L Bonadonna ◽

R Briancesco ◽

C Martini ◽

...

Keyword(s):

Comparative Study ◽

Sodium Hypochlorite ◽

Peracetic Acid ◽

Pilot Plant ◽

Wastewater Disinfection

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Influence of different sanitizers on food contaminant bacteria: effect of exposure temperature, contact time, and product concentration

Food Science and Technology ◽

10.1590/s0101-20612012005000046 ◽

2012 ◽

Vol 32 (2) ◽

pp. 228-232 ◽

Cited By ~ 7

Author(s):

Cezar Augusto Beltrame ◽

Gabriela Busnello Kubiak ◽

Lindomar Alberto Lerin ◽

Ieda Rottava ◽

Altemir José Mossi ◽

...

Keyword(s):

Organic Acids ◽

Peracetic Acid ◽

Quaternary Ammonium ◽

Contact Time ◽

Industrial Plant ◽

Meat Industry ◽

Product Concentration ◽

E Coli ◽

Exposure Temperature ◽

Different Temperatures

The efficiency of four Sanitizers - peracetic acid, chlorhexidine, quaternary ammonium, and organic acids - was tested in this work using different bacteria recognized as a problem to meat industry, Salmonella sp., S. aureus, E. coli and L. monocytogenes. The effects of sanitizer concentration (0.2, 0.5, 0.6, 1.0, 1.1 and 1.4%), at different temperatures (10 and 45 °C) and contact time (2, 10, 15, 18 and 25 minutes) were evaluated. Tests in an industrial plant were also carried out considering previously obtained results. In a general way, peracetic acid presented higher efficiencies using low concentration (0.2%) and contact time (2 minutes) at 10 °C. The tests performed in industrial scale showed that peracetic acid presented a good performance in concentration and contact time lower than that suggested by the suppliers. The use of chlorhexidine and quaternary ammonium led to reasonable results at the indicated conditions, and organic acids were ineffective under concentration and contact time higher than those indicated by the suppliers in relation to Staphylococcus aureus. The results, in general, show that the choice for the most adequate sanitizer depends on the microorganism contaminant, the time available for sanitizer application, and also on the process cost.

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