scholarly journals Gender Peculiarities of Protein and Lipid Peroxidation of the Oral Liquid Under the Conditions of Preclinical Iodine and Iron Deficiency

2021 ◽  
Vol 27 (1) ◽  
Author(s):  
Olga Zayats
Keyword(s):  
Lipid Peroxidation ◽  
Iron Deficiency ◽  
Oral Fluid ◽  
Thiobarbituric Acid ◽  
Oxidative Modification ◽  
Peroxidation Of Lipids ◽  
Diene Conjugates ◽  
Endogenous Factors ◽  
Dental Pathology ◽  
Oral Liquid

The prevalence of somatic pathology in children increases significantly with each passing year, which is associated with socio-economic, environmental conditions, the complex interaction of exogenous and endogenous factors. This problem may be related to micronutrient imbalances, among which iodine deficiency and sideropenia are quite common. Objective. The aim of the study is to learn the peculiarities of peroxidation of proteins and lipids, the system of antioxidant defence of oral fluid in conditions of preclinical iodine and iron deficiency. Materials and Methods. The study included 115 children aged 6-18 years. The level of peroxidation of proteins was characterized by the content of oxidative modification of protein products (aldehyde derivatives of the main nature) in the oral fluid. Peroxidation of lipids of oral fluid was assessed by the accumulation of diene conjugates (DCs) of polyunsaturated fatty acids and products responding to thiobarbituric acid (TBA-RP). Results. It has been determined that in case of iodine and iron deficiencies, their combination causes significant activation of protein and lipid peroxidation processes in the oral fluid of children, accompanied by an imbalance of the prooxidant-antioxidant system of the oral cavity. Conclusion. Such changes can be predictors of the development of dental pathology

THE INTENSITY OF OXIDATIVE MODIFICATION OF PROTEINS AND PEROXIDATION OF LIPIDS IN THE BLOOD SERUM, BRAIN, LIVER AND PANCREAS TISSUES OF RATS WITH INSULIN RESISTANCE AND CONGENITAL IODINE DEFICIENCY

2019 ◽  
pp. 115-120
Author(s):  
V. B. Stetsevyat ◽  
N. M. Voronych-Semchenko
Keyword(s):  
Insulin Resistance ◽  
Iodine Deficiency ◽  
Thiobarbituric Acid ◽  
Oxidative Modification ◽  
Peroxidation Of Lipids ◽  
Diene Conjugates ◽  
Oxidative Modification Of Proteins ◽  
High Fructose ◽  
Liver And Pancreas ◽  
Dependent Processes

The results of the study about the nature of oxygen-dependent processes in rats that were on a high-fructose diet for 8 weeks under conditions of adequate iodine supply and congenital iodine deprivation is presented in the article. The significant activation of peroxide oxidation of proteins (by increasing the aldo- and keto-derivates of a neutral nature) and lipids (increased of diene conjugates content and products, that are responsible to thiobarbituric acid) of animals with insulin resistance was found. An aggravating factor in the course of these processes is a congenital iodine deficiency. The changes of oxidative modification of proteins in animals with congenital iodine deficiency had multidirectional character, and processes of lipid peroxidation mainly increased. The most pronounced changes of the studied processes were observed in the liver and pancreas of animals with insulin resistance against the background of congenital iodine deficiency.

THE INTENSITY OF OXIDATIVE MODIFICATION OF PROTEINS AND PEROXIDATION OF LIPIDS IN THE BLOOD SERUM, BRAIN, LIVER AND PANCREAS TISSUES OF RATS WITH INSULIN RESISTANCE AND CONGENITAL IODINE DEFICIENCY

2019 ◽  
pp. 115-120
Author(s):  
V. B. Stetsevyat ◽  
N. M. Voronych-Semchenko
Keyword(s):  
Insulin Resistance ◽  
Iodine Deficiency ◽  
Thiobarbituric Acid ◽  
Oxidative Modification ◽  
Peroxidation Of Lipids ◽  
Diene Conjugates ◽  
Oxidative Modification Of Proteins ◽  
High Fructose ◽  
Liver And Pancreas ◽  
Dependent Processes

The results of the study about the nature of oxygen-dependent processes in rats that were on a high-fructose diet for 8 weeks under conditions of adequate iodine supply and congenital iodine deprivation is presented in the article. The significant activation of peroxide oxidation of proteins (by increasing the aldo- and keto-derivates of a neutral nature) and lipids (increased of diene conjugates content and products, that are responsible to thiobarbituric acid) of animals with insulin resistance was found. An aggravating factor in the course of these processes is a congenital iodine deficiency. The changes of oxidative modification of proteins in animals with congenital iodine deficiency had multidirectional character, and processes of lipid peroxidation mainly increased. The most pronounced changes of the studied processes were observed in the liver and pancreas of animals with insulin resistance against the background of congenital iodine deficiency.

CHANGE IN THE QUANTITATIVE CONTENT OF DIENE CONJUGATES IN THE ORAL FLUID IN RATS UNDER THE EXPOSURE OF TETRAMETHYLTHIURAM DISULFIDE

2020 ◽  
Author(s):  
Лилия Александровна Ячменева ◽  
Владимир Анатольевич Королев ◽  
Елена Викторовна Фелькер ◽  
Иван Владимирович Королев
Keyword(s):  
Lipid Peroxidation ◽  
Toxic Effect ◽  
Oral Fluid ◽  
Diene Conjugates ◽  
Tetramethylthiuram Disulfide ◽  
Lipid Peroxidation Products ◽  
Quantitative Content

Целью исследования было установить уровень диеновых конъюгатов в ротовой жидкости при интоксикации тетраметилтиурамдисульфидом. В результате было установлено, что на 28 сутки был выявлен наибольший токсический эффект ТМДТ, который превысил контрольные значения в 4 раза (400%). Концентрация ДК на 7, 14, 21 сутки возросла на 158,3%, 225,0%, 258,3% соответственно. Полученные результаты говорят о накоплении продуктов перекисного окисления липидов диеновых конъюгатов с увеличением сроков пестицидной интоксикации. The aim of the study was to determine the level of diene conjugates in the oral fluid during tetramethylthiuram disulfide intoxication. As a result, it was found that on day 28, the greatest toxic effect of TMDT was detected, which exceeded the control values by 4 times (400%).. The concentration of DC on days 7, 14, and 21 increased by 158.3%, 225.0%, and 258.3%, respectively. The results obtained indicate the accumulation of lipid peroxidation products of diene conjugates with an increase in the duration of pesticide intoxication.

scholarly journals Changes of Prooxidant-Antioxidant Systems in Experimental Acute Pancreatitis

2017 ◽  
Vol 24 (3) ◽  
Author(s):  
Viktoria Cherkasova ◽  
Luibomyr Zaiats
Keyword(s):  
Lipid Peroxidation ◽  
Acute Pancreatitis ◽  
White Male ◽  
Thiobarbituric Acid ◽  
Activity Level ◽  
Male Rats ◽  
Antioxidant Systems ◽  
Diene Conjugates ◽  
Pronounced Increase ◽  
Oxidation Rates

Mortality in acute destructive pancreatitis, despite the development and introduction of new methods of treatment, remains stable high and in severe forms reaches 25-85%. Activation of neutrophils and macrophages in acute pancreatitis leads to an "oxygen burst", which is closely linked with the activation of lipid peroxidation.Goals. The purpose is to establish dynamic changes in the indexes of prooxidant-antioxidant systems in acute L-arginine-induced pancreatitis.Materials and methods. The study was performed on 62 white male rats of Wistar line weighing 180-220g, with modeled acute pancreatitis. Blood for analysis have been taken: the blood serum on 12, 24, 48 and 72 hours of experiment to determine the activity level of thiobarbituric acid products, diene conjugates, catalase and lactate for assessment of the intensity of oxidative stress and antioxidant systems.Results. The obtained results of the study showed that acute L-arginine-induced pancreatitis is accompanied by an intensification of lipid peroxidation processes (LPO). Revealed that the most pronounced increase in all blood parameters is observed 24 hours after the beginning of the study. A significant increase in the active products of tiobarbituric acid (TBA-AP) and diene conjugates (DC) was detected - 1.98 and 2.7 times, respectively, and 2.2 times the growth of catalase (CT). At the next stage of the experiment there is a slowdown in the rate of LPO, as evidenced by the following values. Thus, for 48 years in the 3rd group: TBA-AP - they increased by 5.1% (p> 0.05), DC - by 3.3% (p> 0.05), and the level of CT - by 43.4% (P <0.05), compared with data for 24 hours. It is important to note that at 72 hours, the CT level decreased by 23.3% (p> 0.05), which may indicate an exhaustion of antioxidant systems. Indicators of LPO on 72 hours compared with 48 hours in group III: TBA-AP - increased by 1.7% (p> 0.05), DC - by 5.7% (p> 0.05).Conclusions. Acute L-arginine-induced pancreatitis is accompanied by an intensification of lipid peroxidation-oxidation processes that can potentiate the development of multiple organ failure in pancreatic inflammation. The most pronounced changes in lipid peroxidation-oxidation rates are observed for 24 hours of study.

scholarly journals Role of lipid peroxidation in pathogenesis of senile cataract

2009 ◽  
Vol 66 (5) ◽  
pp. 371-375 ◽  
Author(s):  
Bojana Kisic ◽  
Dijana Miric ◽  
Lepsa Zoric ◽  
Ilija Dragojevic ◽  
Aleksandra Stolic
Keyword(s):  
Lipid Peroxidation ◽  
Thiobarbituric Acid ◽  
Diene Conjugates ◽  
Lipid Peroxidation Products ◽  
Mature Cataract ◽  
Structure Changes ◽  
Biochemical Research ◽  
Maturity Degree ◽  
Optical Change

Background /Aim. Cataract is a structural, biochemical and optical change in the eye lens, which changes transmission and refraction of light rays reducing keenness and clarity of a figure on the retina. Its occurrence is highest in older people, over the age of 65 (45.9%), thus a certain degree of opacification exists practically in all people over the 70. Our research was directed to measuring of lipid peroxidation products in cataract lenses involved in early stages of cataractogenesis through oxidative stress and in the development of mature cataract. Methods. Clinical and biochemical research was carried out in 101 patients with cataract, 46 women and 55 men. The average age of the group was 72.47 (? = 7.98). According to the cataract maturity degree the patients were classified into two groups as follows: cataracta senilis incipiens (n = 41) and cataracta senilis matura (n = 60). Measuring of diene conjugates was carried out by spectrophotometer. Fluorescent lipid peroxidation products were measured by a spectrofluorophotometer, and malondialdehyde (MDA) concentration was measured by colorimeter as a product of a reaction with thiobarbituric acid (TBA). Result. Significantly higher diene conjugated concentration in lenses was measured in the patients with the diagnosis cataracta senilis incipiens (p < 0.001) as well as the intensity of fluorescent iminopropens (p < 0.001). Significantly higher MDA concentration in lens (p < 0.001) was measured in the patients with cataracta senilis matura. Conclusion. The lens structure changes caused by lipid peroxidation can, with other risk factors present, influence the occurrence and development of mature cataract. Some cataract types show different lipid peroxidation intensity with the most distinct changes in cataract which started as corticonuclear.

scholarly journals LIPID PEROXIDATION AND OXIDATIVE PROTEIN PRODUCTS IN CHILDREN WITH EPISODIC FEVER OF UNKNOWN ORIGIN / LIPIDNA PEROKSIDACIJA I OKSIDATIVNI PROTEINSKI PRODUKTI KOD DECE SA EPIZODINOM GROZNICOM NEPOZNATOG UZROKA

2013 ◽  
Vol 33 (2) ◽  
pp. 197-202 ◽  
Author(s):  
Jelena Radović ◽  
Jelena Vojinović ◽  
Vladmila Bojanić ◽  
Tatjana Jevtović-Stoimenov ◽  
Gordana Kocićs ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Fever Of Unknown Origin ◽  
Plasma Concentrations ◽  
Unknown Origin ◽  
Thiobarbituric Acid ◽  
Well Being ◽  
Oxidative Modification ◽  
Healthy Children ◽  
Oxidative Stress Biomarkers ◽  
Activated Neutrophils

Summary Background: Episodic fever syndromes are commonly seen in pediatric practice. Episodic fever of unknown origin (FUO) lasts for a few days or weeks and is followed by a fever-free period with a sense of well-being. In this condition, activated neutrophils and monocytes intensively generate reactive oxidative species that may further damage various mole- cules. The aim of the study was to evaluate oxidative stress biomarkers, lipid peroxidation in erythrocytes and plasma, and advanced oxidation protein products (AOPP) in children with episodic FUO. Methods: The study enrolled 25 children with episodic FUO in afebrile phase and 25 healthy children as controls. Lipid peroxidation was evaluated by measuring malondialdehyde (MDA) production with the thiobarbituric-acid-reactive sub- stances (TBARS) assay in erythrocytes and plasma. Oxidative modification of proteins was measured spectrophotometri- cally by the determination of AOPP in plasma. Results: Mean duration of episodic fevers was 3.96±2.8 years. Erythrocyte MDA levels were higher in children with FUO than in controls (86.26± 10.75 vs. 78.0±3.21 nmol/g hemoglobin), although not significantly (p=0.202). The MDA plasma concentrations were similar (2.42±0.35 vs. 2.41 ±0.39 (xmol/L) between the groups (p=0.732). Unexpectedly, levels of AOPP were significantly lower in chil- dren with FUO than in healthy controls (18.8±5.04 vs. 25.1 ±3.35 nmol/L, p=0.047). Conclusions: Episodic fevers of unknown origin with an aver- age duration of 3.96±2.8 years do not cause significant oxidative modifications of lipids and proteins in children.

scholarly journals Study of prooxidant activity and some indicators of the pituitary-gonadal system in patients suffering from chronic endometritis

2020 ◽  
Vol 22 (1) ◽  
pp. 66-69
Author(s):  
N V Kirillova ◽  
O M Spasenkova ◽  
A G Platonova
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Protein Modification ◽  
Aliphatic Aldehyde ◽  
Oxidative Modification ◽  
Control Group ◽  
Diene Conjugates ◽  
Oxidative Modification Of Proteins ◽  
Chronic Endometritis ◽  
Oxidative Protein Modification

The state of lipid peroxidation and the oxidative modification of proteins in the blood of 29 women with chronic endometritis aged 27 to 40 years were studied. The control group consisted of 19 healthy women aged 27 to 44 years. The intensity of lipid peroxidation was estimated by the level of diene conjugates and malondialdehyde, the oxidative modification of proteins - by the level of carbonyl derivatives. It was shown that in the blood of women with chronic endometritis, the concentration of aliphatic aldehyde groups and 1,9 times the carbonyl groups in protein molecules increases by 3,3 times compared with the control group. A significant increase in diene conjugates by 34% and malondialdehyde in blood plasma and red blood cells by 47 and 43%, respectively, was established. The increase in lipid peroxidation products and oxidative protein modification in the blood indicates the development of oxidative stress in women suffering from chronic endometritis. Compared with healthy patients with chronic endometritis, a convincing tendency toward an increase in prolactin and anti- Muller hormone against a decrease in progesterone was revealed in the group compared to healthy ones. To predict possible complications in patients with chronic endometritis, it is advisable to study the indicators of protein and lipid peroxidation as markers of oxidative stress and the severity of the inflammatory process. In addition, such patients should be advised to take antioxidant drugs in combination with the basic therapy.

Sulfite Supported Lipid Peroxidation in Propofol Emulsions

2002 ◽  
Vol 97 (5) ◽  
pp. 1162-1167 ◽  
Author(s):  
Max T. Baker ◽  
Deborah J. Dehring ◽  
Marc S. Gregerson
Keyword(s):  
Lipid Peroxidation ◽  
Ascorbic Acid ◽  
Soybean Oil ◽  
Thiobarbituric Acid ◽  
Glass Electrode ◽  
Peroxidation Of Lipids ◽  
Sodium Metabisulfite ◽  
Oil Emulsion ◽  
Nitrobenzoic Acid ◽  
Oil Emulsions

Background Sodium metabisulfite is added to a commercial propofol emulsion as an antimicrobial agent. The sulfite ion (SO3(-2)) is capable of undergoing a number of reactions, including autooxidation and the promotion of lipid peroxidation. This study evaluated sulfite reactivity in propofol emulsions by determining thiobarbituric acid reacting substances (TBARS), sulfite depletion, and emulsion pH in emulsions containing sulfite or EDTA. Methods Commercial EDTA and sulfite propofol emulsions were compared, and 10% soybean oil emulsion containing various additives were evaluated for TBARS, sulfite, and pH. TBARS were analyzed with a modified thiobarbituric acid method. Sulfite was analyzed by the reaction of sulfite with 5,5'-dithiobis(2-nitrobenzoic acid). pH was measured by glass electrode methodology. Results Thiobarbituric acid reacting substances were detectable in commercial sulfite propofol emulsions in concentrations ranging from 0.02 to 0.22 microg/ml based on malondialdehyde. No TBARS were detected in EDTA propofol emulsions. Incubation (37 degrees C, up to 6 h) of sulfite propofol emulsions in air resulted in further increases in TBARS (35-160%). No increases occurred in incubated EDTA propofol emulsions. Metabisulfite (0.25 mg/ml) alone added to 10% soybean oil resulted in large increases in TBARS that were inhibited in part by propofol (10 mg/ml) and completely by ascorbic acid (0.05 mg/ml). Soybean oil emulsion pH declined rapidly on the addition of metabisulfite (0.25 mg/ml). The addition of propofol (10 mg/ml) partially inhibited the decline in pH and ascorbic acid (0.05 mg/ml) completely inhibited it. Conclusion These results show that sulfite supports the peroxidation of lipids in soybean oil emulsions and propofol functions to partially inhibit these processes.

scholarly journals PROCESSES OF LIPID PEROXIDATION AND ANTIOXIDANT PROTECTION IN ACUTE ODONOGIC OSTEOMYELITIS IN CHILDREN

2020 ◽  
Vol 20 (1) ◽  
pp. 67-71
Author(s):  
P.I. Tkachenko ◽  
N.M. Lokhmatova ◽  
O.B. Dolenko ◽  
Yu.V. Popelo ◽  
N.M. Korotych
Keyword(s):  
Lipid Peroxidation ◽  
Clinical Examination ◽  
Peripheral Blood ◽  
Oral Fluid ◽  
Antioxidant Protection ◽  
Significant Deterioration ◽  
Diene Conjugates ◽  
Fluid Fraction ◽  
Therapeutic Measures ◽  
Peroxidation Processes

The paper presented summarizes the results on the parameters of free radical lipid oxidation and antioxidant protection factors in 24 children aged from 3 to 15 years with acute odontogenic mandible osteomyelitis. In addition to standard clinical examination, peripheral blood and unstimulated oral fluid fractions were collected from all patients for 10 min at hospital admission and discharge. The samples were used to determine the components of lipid peroxidation: diene conjugates; malondialdehyde, its initial level, in 1.5 hours of incubation and its growth; antioxidant protection factors as superoxide dismutase, and catalase. It has been found out at the time of hospitalization, clinical examination showed a significant deterioration of the general condition, which was accompanied by widespread infiltrative changes and collateral oedema in the projection of the involved region of the mandible. In 12 people (50%) osteomyelitis spread from temporary molars. In other cases, permanent first and second molars were regarded as the causes. The patients demonstrated the activation of lipid peroxidation processes and reduction of antioxidant protection factors found out in peripheral blood and unstimulated oral fluid fraction. Repeated studies over the clinical recovery following the treatment conducted in accordance with the protocol of medical care enables to estimate the absence of infiltrative changes, but despite the elimination of acute inflammation, not all the studied parameters reached the level of control values. This makes it necessary to address the issue on making corrections towards the complex of therapeutic measures by including antioxidants that seems to be quiet promising and requires further in-depth investigation.

The evaluation of lipid peroxidation and oxidative modification of proteins in blood serum under obesity development and the consumption of aqueous kidney beans Phaseolus vulgaris pods extract

2020 ◽  
Vol 33 (1) ◽  
pp. 38-44
Author(s):  
Alona Yurchenko ◽  
Daryna Krenytska ◽  
Olexii Savchuk ◽  
Tetiana Halenova ◽  
Natalia Raksha ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Oxidative Modification ◽  
Resistance Development ◽  
Oxidative Modification Of Proteins ◽  
Kidney Beans ◽  
Calorie Diet ◽  
Diet Control ◽  
High Calorie Diet ◽  
High Calorie

AbstractOur interest has focused on the investigation of the anti-obese potential of kidney beans (P. vulgaris) pods extract. In the course of the study, obesity development in rats was induced with high-calorie diet. Control and obese rats then have consumed with aqueous kidney beans (P. vulgaris) pods extract during 6 weeks (200 mg/kg). Results show that the long-term consumption of P. vulgaris pods extract can lead to the reduction of hyperglycemia and insulin resistance development. Furthermore, we saw a normalization of lipid peroxidation parameters and oxidative modification of protein due to the consumption of the kidney beans (P. vulgaris) pods extract. Our experimental data demonstrate the ability of the kidney beans (P. vulgaris) pod extracts to mitigate obesity development but the details of this mechanism remains to be not fully understood.

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