scholarly journals Histomorphological Effects of Nicotine on Selected Parts of the Brain of Adult Wistar Rats

2018 ◽  
Vol 25 (2) ◽  
Author(s):  
John Chukwuma Oyem ◽  
Emmanuel Igho Odokuma
Keyword(s):  
Substantia Nigra ◽  
Cell Count ◽  
Wistar Rats ◽  
Neuronal Cell ◽  
Oral Exposure ◽  
Control Group ◽  
Cell Diameter ◽  
Dependent Manner ◽  
Histological Techniques ◽  
Group 1

Nicotine has been defined as a potent parasympathomimetic alkaloid that accumulates in the roots and leaves of Nightshade family of plants Aim: This study was aimed at evaluating the effects of orally ingested nicotine in the histology of hippocampus, substantia nigra and cerebellum.Materials and Methods: Twenty four adult male Wistar rats (100g – 200g) were randomly divided into 4 groups (group 1 – group 4). Group 1 served as the control group, while groups 2 - 4 were the treated groups. Nicotine was diluted in water and 1ml of the different dosage (2mg/kg/day, 4mg/kg/day and 6mg/kg/day) were administered to the treated groups respectively with the aid of orogastric cannula for 42 days. Animals were euthanized by cervical dislocation at the end of 7, 21 and 42 days so as to demonstrate the dose and time dependant effect of this agent. Brain tissues were harvested, processed and stained using Haematoxylin and eosin according to standard histological techniques. Stained tissue images were captured using digital micrometer eyepiece and cell count was determined using stereological technique.Statistical analysis: Data obtained were subjected to statistical analysis with the use of statistical package for social sciences (SPSS version 20). Significant differences were obtained using One Way Analysis of Variance with a probability of  0.05 (95% confidence limit) and Tukeys post hoc  test was further used to determine the mean significant differences between specific groups.Results: Histological findings showed mild, moderate and severe hyperplasia in a dose and time dependant manner. However, observations from quantitative analysisalso revealed a dose and time dependant significant increase in neuronal cell count and cell diameter of the hippocampus, Substantia nigra and cerebellum.Conclusion: This study has demonstrated that oral exposure of Nicotine in rats display proliferative adaptive changes on the hippocampus, substantia nigra and cerebellum in a dose/time dependent manner.

The Effects of the "XGTQ" Medicine in Treating Cirrhosis in Wistar Rats

2020 ◽  
Vol 06 ◽  
Author(s):  
Ngan Nguyen Hoang ◽  
Thang Duong Minh ◽  
Tuan Anh Hoang ◽  
Son Le Ngoc Bich ◽  
Duong Nguyen Huu ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Reference Group ◽  
Control Group ◽  
Plain Water ◽  
Group 4 ◽  
White Rats ◽  
Collagen Accumulation ◽  
Activity Of Enzymes ◽  
Group 3 ◽  
Group 1

Objectives: Evaluate the effects of "XGTQ" in the treatment of cirrhosis induced by Carbon tetrachloride (CCL4) in combination with alcohol and high-fat diet on Wistar rats. Materials and methods: Cirrhosis on white rats was induced by subcutaneously injecting CC14 at an initial dose of 5,0ml/kg, followed by 1,2ml/kg once a week in 10 weeks. Then, fed with synthetic food, added 20% fat, and 0.05% cholesterol and iron oxalate. Rats were administered every day with plain water and 1 day with water mixed with 30% ethanol. The rats were randomly divided into 5 groups and given distilled water (group 1 and 2 or control group), silymarin (group 3 or reference group) or the "XGTQ" drug extract (group 4, 5) for 4 weeks. Collected blood for biochemical test and liver were dissected to evaluate weight, morphology and quantified 4-hydroxyproline to evaluate fibrosis and collagen accumulation. Results: In cirrhotic wistar rats, "XGTQ" drug at 19.6 g/kg/24h and 58.8 g/kg/24h showed the ability of reducing the activity of enzymes AST, ALT in the blood (p<0.01), increasing plasma albumin and decreasing prothrobin time (p<.05); improving physical condition, macroscopic and microscopic images of H&E-stained liver; decreasing the concentration of hydroxyproline in the liver and reducing the level of cirrhosis on the masson-stained templates. The effects of "XGTQ" increased with the dose, and was equivalent to silymarin at the dose of 70 mg/kg/24h. Conclusion: The extract of "XGTQ" drug is effective in treating cirrhosis in Wistar rats.

scholarly journals Urinary N-Acetyl-Beta-D-Glucosaminidase Activity in Rat Experimental Ischemic and Toxic Models of Acute Kidney Injury

2017 ◽  
Vol 74 (1) ◽  
pp. 105
Author(s):  
Razvan Andrei CODEA ◽  
Mircea MIRCEAN ◽  
Sidonia Alina BOGDAN ◽  
Andras Laszlo NAGY ◽  
Alexandra BIRIS ◽  
...  
Keyword(s):  
Acute Kidney Injury ◽  
Experimental Model ◽  
Wistar Rats ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Kidney Injury ◽  
Control Group ◽  
Tubular Injury ◽  
Group 2 ◽  
Group 1

The identification of a suitable prevention method which facilitates limiting the deleterious effects of acute kidney injuries is highly required. In order to identify a proper treatment for acute kidney injuries, a suitable experimental model that replicates the structural, metabolic and inflammatory lesions that occur in the natural acute injured kidney is highly necessary. Intense urinary NAG activity can be found in a variety of renal disease such as toxic nephropathies, ischemic renal injury following cardiac surgery or renal transplantation but also in glomerular disease especially in diabetic nephropathy. Rises in urinary NAG enzyme activity strongly suggests tubular cell damage and support NAG enzyme as a biomarker of renal tubular injury. The aim of this paper is to obtain a stable in vivo acute kidney injury experimental model, in Wistar, rats and to evaluate the urinary activity of N-acetyl-β-D-glucosaminidase (NAG) enzyme, blood levels of urea and creatinine and microstructural renal alterations induced by ischemia/reperfusion injury respectively gentamicin nephrotoxicity. For this purpose we have used a rat experimental model. Adult male Wistar rats weighing 250-300 g were randomly divided into 3 groups with 8 rats in each group. Group 1 served as a model for the renal ischemia/reperfusion injury experiment, group 2 served for toxic kidney injury experimental model and group 3 served as control group. All individuals in both groups 1 and 2 presented marked elevations in blood urea and creatinine at the moment of euthanasia (day 3 for group 1 and day 9 for group 2) compared to the control group where biochemical values remained within normal limits. Urine analysis of both group 1 and 2 showed marked urinary NAG index activity which suggests acute tubular injury, suggestion confirmed by histological evaluation of the renal parenchyma sampled from this subjects

Effects of hydroxyl-functionalized multiwalled carbon nanotubes on sperm health and testes of Wistar rats

2017 ◽  
Vol 33 (6) ◽  
pp. 519-529 ◽  
Author(s):  
NK Nirmal ◽  
KK Awasthi ◽  
PJ John
Keyword(s):  
Carbon Nanotubes ◽  
Wistar Rats ◽  
Chemical Properties ◽  
Control Group ◽  
Dependent Manner ◽  
Multiwalled Carbon ◽  
Treatment Groups ◽  
Significant Impairment ◽  
Functionalized Multiwalled Carbon Nanotubes ◽  
Male Reproductive Health

Carbon nanotubes (CNTs) are promising candidates for various applications including biomedical purposes. Owing to their remarkable physical, mechanical, electrical and chemical properties, CNTs have become an area of intense research and industrial activity in recent years. Therefore, toxicity and risk assessment studies are becoming increasingly important. The present study was designed to assess the effects of hydroxyl-functionalized multiwalled CNTs (OH-f MWCNTs) on sperm health and testes of adult Wistar rats. Animals were treated with different doses of OH-f MWCNTs (0.4, 2.0 and 10.0 mg/kg) along with a control group receiving only vehicle. Assessments after 15 alternate intraperitoneal doses revealed dose-related adverse effects on many endpoints tested. Results of the study showed significant impairment of sperm health at 2.0 and 10.0 mg/kg. Histology of testes demonstrated degeneration of germinal epithelium and loss of germ cells in the treatment groups. The exposure resulted in increased oxidative stress in testes in a dose-dependent manner. The findings of the study demonstrate that CNTs are potentially harmful for male reproductive health.

scholarly journals Histomorphological evaluation of osteoclast cell count in femur bone of mice induced by anastrazole and protective effect of olive oil.

2021 ◽  
Vol 28 (12) ◽  
pp. 1837-1843
Author(s):  
Anjum Ishaque ◽  
Saima Nadeem ◽  
Shagufta Nisar ◽  
Hasnain Ali Shah ◽  
Khalid Javed ◽  
...  
Keyword(s):  
Experimental Study ◽  
Bone Loss ◽  
Olive Oil ◽  
Cell Count ◽  
Control Group ◽  
Femur Bone ◽  
Group 3 ◽  
Albino Mice ◽  
Group 2 ◽  
Group 1

Objective: The objective of this study is to find out protective effect of olive oil to prevent bone loss by decreasing osteoclast count in patient receiving Anastrazole. Study Design: Experimental study. Setting: Pakistan Council for Scientific and Industrial Research (PCSIR) Animal House, Peshawar and Pathology Lab KGMC Peshawar. Period: March 2019 to December 2019. Material & Methods: Sixty female albino mice 6-8 weeks of age were selected for this experimental study and Aromatase inhibitor drug Anastrazole was given alone and in combination with olive oil once daily for 30 successive days. Femur bone samples were collected and stained with Eosin and Hematoxylin for histomorphological evaluation of osteoclast cell count in three all three groups i.e. control group, those receiving Anastrazole alone and those given Anastrazole and olive oil in combination. Results: The mean weight of all experimental female albino mice before study was 30.77- 33.05 grams and after the study was 30.84- 21.31 grams. Control group 1 which was given normal diet showed increased weight of mice with less osteoclast cell count as compared to experimental groups (2 and 3).  In group 2 (Drugged) which was given Anastrazole, weight of were lesser than control group 1 and group 3(Anastrazole + olive oil), while, osteoclast score was greater than group 1(control) and group 3 (Anastrazole + olive oil). Group3 (Drugged+ Olive oil) showed greater weight of mice than group 2 (Anastrazole) but, lesser than control group 1. Osteoclast score was greater than control group but lesser than group 2 (Anastrazole). Conclusion: The results showed positive and protective effects of olive oil against Anastrazole induced bone loss in female albino mice.

scholarly journals The Green Bitter Weed (Hymenoxys odorato) Plant Extract Is Toxic to the Liver and Kidney of Wistar Rats

2018 ◽  
Author(s):  
John Juma Ochieng ◽  
Isaac Echoru ◽  
Musa Ajibola Iyiola
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Complete Blood Count ◽  
Medical Personnel ◽  
Control Group ◽  
Dependent Manner ◽  
Snake Bites ◽  
Male Wistar Rats ◽  
Liver And Kidney ◽  
High Doses

Background: Medicinal plants are of great importance to health of individual and communities. About 80% of the population in Uganda relies on traditional medicine because western-trained medical personnel are limited especially in villages. Most Ugandans use Hymenoxys odorato for medicinal purposes e.g. to treat colds, fever, coughs, anti-helminthes, locally used as tea, anti-allergy and also as an anti-venom to relieve snake bites. Method: A group of 25 male wistar rats of 150 g&ndash;210 g were kept for 14 days while being fed and treated with the extract. At 14th day, anesthesia was given and blood samples collected by cardiac puncture for hematological and biochemical investigations. Serum was analyzed for Alkaline Phosphatase, Aspartate Transaminase and Alanine Transaminase while whole blood was used for complete blood count. The liver and kidney were removed and placed in 10% formalin to prepare for histology staining using haematoxylin and eosin technique. Results: The extract elevated hepatic biomarker enzymes i.e. ALP, ALT and AST. The increase was found to be significantly different (P &gt; 0.05) at 400 and 500 mg/kg doses as compared to the control group. Histological sections of the liver showed distortion of liver cytoarchitecture, steatosis, necrosis of hepatocytes and congestion of the sinusoids at high doses 300, 400 and 500 mg/kg body weight. In the sections of the kidney, there was mild distortion of the integrity of the kidney with glomerular hypercellularity at high doses (400 and 500 mg/kg per body weight). Conclusion: Hymenoxys odorato aqueous extract has toxic effects on the liver and kidney of wistar rats. The effects were observed to be in a dose dependent manner.

scholarly journals Cypermethrin and chlorpyrifos raises serum urea level and causes abnormal sperm morphology in Wistar rats

2019 ◽  
Vol 9 (3) ◽  
pp. 3969-3973 ◽  
Keyword(s):  
Wistar Rats ◽  
Sperm Morphology ◽  
Biochemical Properties ◽  
Oral Exposure ◽  
Control Group ◽  
Serum Urea ◽  
Rat Serum ◽  
Toxicological Effect ◽  
Abnormal Sperm ◽  
Male Wistar Rats

Chlorpyrifos (organophosphate) and cypermethrin (pyrethroid) are insecticides, which are widely used for agricultural as well as for domestic purposes. This study investigated the toxicological effect of chlorpyrifos and cypermethrin on selected organs and tissues of male Wistar rats. Nine (9) male Wistar rats were randomly grouped into three and were orally given chlorpyrifos or cypermethrin, while the control group was given distilled water for 28 days. The results revealed a significant increase (p<0.05) in rat serum AST activity for the chlorpyrifos and cypermethrin groups. Also, there was significant elevation in serum urea following oral exposure to either chlorpyrifos or cypermethrin. Conversely, a reduction in the rat liver ALP activity for treatment with cypermethrin or chlorpyrifos was recorded. The histology results revealed that the administration of chlorpyrifos but not cypermethrin for 28 days has no significant effect on the biochemical properties and sperm morphology of the rats. Taken together, findings indicate that cypermethrin and chlorpyrifos exposure in rats predisposes to renal injury, while altering sperm morphology.

scholarly journals Developmental changes in frontocortical morphology and neurochemistry of neonatal rats following gestational nicotine exposure

1970 ◽  
Vol 6 (2) ◽  
pp. 969-976
Author(s):  
Gabriel Olaiya Omotoso ◽  
Adeolu Stephen Alabi ◽  
Oluwole Busayo Akinola ◽  
Bernard Ufuoma Enaibe
Keyword(s):  
Frontal Cortex ◽  
Wistar Rats ◽  
Developmental Changes ◽  
Neurological Deficits ◽  
Control Group ◽  
Nicotine Exposure ◽  
Nicotine Administration ◽  
Prenatal Nicotine ◽  
Group 1

Exposure of the embryo or foetus to nicotine during development results in some forms of neurological deficits later in life. The current study aimed at determining the effects of prenatal nicotine administration during the 1st and 2nd gestational weeks on the frontal cortex of neonatal Wistar rats. For each week of gestation, pregnant Wistar rats were assigned to 3 groups: a control group (1), and two treated groups (2 and 3), and were given intra-peritoneal nicotine at 6.88 mg/ kg and 13.76 mg/kg doses respectively. The weights of the litters were taken at birth and at postnatal day 4; the whole brain and frontal cortical weights were also assessed. Tissues for histological demonstration were fixed in freshly prepared formol calcium, while specimen for biochemical studies were homogenised and processed for the determination of alkaline phosphatase (ALP) and malondialdehyde (MDA) activities. Findings in the treated animals showed low birth weights, raised ALP but reduced MDA, with corresponding alterations in the cortical cytoarchitecture, which could explain some of the pathological basis for the neurobehavioural problems associated with offspring of women smokers.Keywords: Prenatal nicotine, Frontal cortex, Morphology, Histology, ALP, MDA

scholarly journals Effect of ovarian autotransplantation on FSH levels in Wistar rats late menopause model

2018 ◽  
Vol 26 (1) ◽  
pp. 42
Author(s):  
Abdurahman Laqif ◽  
Dewi Kartika DJ Anwar ◽  
Eriana Melinawati
Keyword(s):  
Data Analysis ◽  
Wistar Rats ◽  
Control Group ◽  
Bilateral Oophorectomy ◽  
Group 3 ◽  
The Mean ◽  
Group 2 ◽  
Post Hoc ◽  
Group 1 ◽  
Elisa Data

Objectives: To determine the effect of ovarian autotransplan-tation on decreasing FSH level in Wistarrats late menopause model.Materials and Methods: Experimental analytic research on 27 Wistar rats (Rattus novergicus) was divided into 3 groups. Group 1 (K1) or control. Group 2 (K2) performed bilateral oophorec-tomy without autotransplantation. Group 3 (K3) performed bilateral oophorectomy with autotransplantation. Measurements of FSH levels were performed on the first day, day 28 (four weeks after bilateral oophorectomy, during late menopause) and 56 day (four weeks after autotransplant). Measurement of FSH levels using ELISA. Data analysis used ANOVA and Post Hoc test.Results: The mean FSH level measured on day 56 at K1 = 63.400 ng/mL, at K2 = 78.416 ng/mL and K3 = 31.991 ng/mL. There were significant differences between K1 and K2 (p = 0,000), K1 and K3 (p = 0,000), and between K2 and K3 (p = 0,000).Conclusion: Ovarian autotransplantation decrease FSH levels in Wistar rats late menopause model.

scholarly journals Hippocampal Glial Degenerative Potentials of Mefloquine and Artequin in Adult Wistar Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-5
Author(s):  
Nsikan-Abasi B. Udoh ◽  
Theresa B. Ekanem ◽  
Moses B. Ekong ◽  
Aniekan I. Peter ◽  
Amabe O. Akpantah
Keyword(s):  
Wistar Rats ◽  
Pyramidal Neurons ◽  
Silver Impregnation ◽  
Control Group ◽  
Distilled Water ◽  
Impregnation Method ◽  
Hippocampal Function ◽  
Oral Doses ◽  
Dose Dependent ◽  
Group 1

Mefloquine and Artequin are two effective antimalarial drugs currently in use in the treatment of uncomplicated malaria. This study was to investigate the hippocampal glial degenerative potentials of these drugs in adult Wistar rats. Forty-nine adult Wistar rats weighing 200 g were divided into groups 1–7. Group 1 served as the control that received distilled water, while groups 2–7 received oral doses of 0.86/1.07 mg/kg, 1.71/2.14 mg/kg, and 3.24/4.28 mg/kg of Artequin and 1.07 mg/kg, 2.14 mg/kg, and 4.28 mg/kg of Mefloquine. The treatment lasted for three days, and on day 4 the animals were sacrificed. Their hippocampi were preserved in neutral formal saline and processed by silver impregnation method. The histomorphology of the hippocampal sections of rats in the groups treated with 2.14 mg/kg and 4.28 mg/kg of Mefloquine and 0.86/1.07 mg/kg, 1.71/2.14 mg/kg, and 3.24/4.28 mg/kg of Artequin showed large and dense populations of astrocytes and astrocytes’ processes, with either loss or reduction in the population of oligodendrocytes. There was also loss in the population of pyramidal neurons all compared with the control group. In conclusion, Mefloquine and Artequin administration induced dose-dependent reactive astrocytes and astrocytes’ processes formation in the hippocampus. This may impair the uptake of neurotransmitter and alter neuronal environment thus altering the hippocampal function.

scholarly journals EVALUATION OF ANTIDEPRESSANT ACTIVITY OF ETHANOLIC EXTRACT OF LAGERSTROEMIA SPECIOSA LEAVES IN ALBINO WISTAR RATS

2019 ◽  
pp. 68-74
Author(s):  
VANITA KANASE ◽  
SUNITA VISHWAKARMA
Keyword(s):  
Oxidative Stress ◽  
Normal Saline ◽  
Restraint Stress ◽  
Wistar Rats ◽  
Ethanolic Extract ◽  
Antidepressant Activity ◽  
Control Group ◽  
Dependent Manner ◽  
Lagerstroemia Speciosa ◽  
Dose Dependent

Objective: The objective of the study was to evaluate the antidepressant activity of ethanolic extract of dried leaves of Lagerstroemia speciosa L. (EELS) on acute restraint stress (ARS)-induced depression-like behavior and biochemical alterations in albino Wistar rats. Methods: Thirty rats were randomly divided into five experimental groups. Group-I (normal control) rats received normal saline (2.0 ml/kg, p.o.) daily for 14 days; Group-II (stress control) rats received normal saline (2.0 ml/kg, p.o.) daily for 14 days and subjected to restraint stress on the 13th day. Group-III (standard drug-treated) rats received imipramine (15 mg/kg, p.o.) daily for 14 days and subjected to restraint stress on the 13th day. Groups-IV and V rats were treated with EELS (100 mg/kg and 300 mg/kg, p.o.) daily for 14 days subjected to ARS on the 13th day. Stress-like behavior was assessed by subjecting the rats to behavioral paradigms such as tail-suspension test (TST) and open field test (OFT), 40 min post-restraint stress procedure. Pretest of 10 min for forced swim test (FST) was also given to each rat simultaneously. Then, 23.5 h later, the relevant samples were administered and the main test performed 30 min later. Oxidative stress parameters such as superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), and extent of lipid peroxidation (LPO) were analyzed in restraint stress-induced animals and control group, following FST on the 15th day. Statistical Analysis: Expression of data was done as a mean standard error of the mean. The normally distributed data were subjected to one-way analysis of variance followed by Dunnett’s test. *p<0.05 was considered statistically significant. Results: It was observed that L. speciosa L. showed a significant dose-dependent decrease in duration of immobility time in TST and FST when compared with the control group in a dose-dependent manner. The results of OFT also showed a dose-dependent increase in locomotor activity. In addition to behavioral tests, EELS also normalized oxidative stress markers such as CAT, SOD, MDA, and LPO in a dose-dependent manner. Conclusion: The results suggest that the ethanolic extract of L. speciosa L. leaves possesses significant antidepressant property, may be recommended as a supplement for the antidepressant activity.

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