scholarly journals Effect of Season on Physico-Morphological Attributes of Cryopreserved Hariana Bull Spermatozoa

2018 ◽  
Vol 14 (1) ◽  
Author(s):  
Vipin Singh ◽  
Anuj Kumar ◽  
Chhote Lal Yadav ◽  
Akhil Patel ◽  
Brajesh Yadav ◽  
...  
Keyword(s):  
Sperm Concentration ◽  
Winter Season ◽  
Morphological Characteristics ◽  
Summer Season ◽  
Morphological Attributes ◽  
Bull Semen ◽  
Sperm Abnormality ◽  
Different Seasons ◽  
Live Sperm ◽  
Acrosomal Integrity

The present study was undertaken to evaluate the effect of seasonal variations on various seminal attributes and freezability of Hariana bull semen. The study was conducted over a period of four months and divided into two peak seasons as winter (December-January) and summer (May-June). Twenty-four ejaculates were collected from 4 bulls in each season. The physico-morphological characteristics of semen, viz., volume (ml), mass activity (0-5 scale), pH, sperm concentration (million/ ml), progressive motility (%), sperm livability (%), HOST (%), total morphological sperm abnormality (%) and acrosomal integrity (%) were estimated at different stages of cryopreservation in different seasons. During summer season the ejaculate volume and seminal pH were found non-significantly higher than in winter, whereas the percentage of morphologically abnormal spermatozoa was significantly (p Lass Than 0.05) higher in summer. Post- thaw percentage of progressively motile spermatozoa was significantly (p Lass Than 0.05) higher in winter than summer season. Live sperm per cent were significantly (p Lass Than 0.05) higher in winter than summer season both at fresh and pre-freeze stage. HOST positive sperm and acrosomal integrity were significantly (p Lass Than 0.01) higher in winter season than summer at all three stages. The study generated basic information that the summer season adversely affects the various physico-morphological characteristics of Hariana bull semen altering its freezability.

scholarly journals Influence of Seasons on Seminal Attributes of Crossbred Bulls and its Implications on Fresh, Diluted and Cryopreserved Semen

2018 ◽  
Vol 14 (1) ◽  
Author(s):  
P. M. Kapale ◽  
A. S. Nagvekar ◽  
P. L. Dhande ◽  
S. D. Ingole ◽  
N. R. Dagli ◽  
...  
Keyword(s):  
Monsoon Season ◽  
Sperm Concentration ◽  
Winter Season ◽  
Egg Yolk ◽  
Summer Season ◽  
Monthly Interval ◽  
Semen Volume ◽  
Microscopic Evaluation ◽  
Live Sperm ◽  
Fresh Semen

The present study was conducted on eight crossbred bulls (HFxGir, 50-75% HF inheritance) under Konkan Development Corporation Ltd. of Maharashtra. Semen ejaculates obtained at monthly interval were used for this study. Semen samples were subjected to routine macroscopic and microscopic evaluation. The study was planned for three seasons, viz., summer, winter and monsoon. The ejaculates were divided into two parts: one part was used for analysis as fresh semen and second part was diluted using egg yolk extender and then cryopreserved. It was observed that the sperm motility and live sperm percentage were significantly low during summer season as compared to winter and monsoon season in fresh semen samples. The semen volume was significantly higher during summer season whereas the sperm concentration was higher in winter season. The colour, consistency and density were not affected by season. Thus, it was concluded that summer season adversely affects the seminal attributes which may result in low quality semen production.

scholarly journals Effect of Age and Frequency of Collection on Quality of Jersey Bulls Semen at National Livestock Breeding Center (NLBC), Nepal

2019 ◽  
Vol 7 (1) ◽  
pp. 88-95 ◽  
Author(s):  
S. Sankhi ◽  
K.R. Sapkota ◽  
B. Regmi
Keyword(s):  
Semen Quality ◽  
Sperm Concentration ◽  
Quality Parameters ◽  
Bull Semen ◽  
Livestock Breeding ◽  
Abnormal Sperm ◽  
Breeding Center ◽  
Live Sperm ◽  
Level Of Significance ◽  
Effect Of Age

Objective: To determine the effect of age and frequency of collection on quality parameters of Jersey bull semen at National Livestock Breeding Center (NLBC), Pokhara. Materials and Methodology: Nine Jersey bulls were selected randomly from NLBC. To obtain the effect of age, six bulls of three different age interval (3-4, 5-7 and 8-9 years) were selected randomly. Remaining three bulls were used to determine the effect of frequency of collection on semen quality. Studies were conducted for three months (Nov/Dec to Dec/Jan, 2016). Semen quality was analyzed using CASA system and SMILE software. ANOVA and paired t- tests were applied to find out level of significance. All the statistical analyses were done using SPSS 17.0 Results: Sperm concentration, post- thaw motility, live sperm, and abnormal sperm were significantly affected by the age of the bulls. Semen quality was found superior in the bulls belonging to 5-7 years of age intervals. Significantly higher value for semen volume, sperm concentration and post- thaw motility was obtained on second collection (P<0.05). However, volume initial motility, pre-filling motility, live sperm and abnormal sperm percentage did not differ significantly among different age intervals and between the frequencies of collection. Conclusion: Different quality parameters of semen like volume, concentration, initial motility, pre-filling motility, post thaw motility, live and normal spermatozoa percentages were higher in 5-7 years old bulls and in second collection of semen. Int. J. Appl. Sci. Biotechnol. Vol 7(1): 88-95

scholarly journals Effect of Age and Frequency of Collection in Quality of Jersey Bulls Semen at National Livestock Breeding Center, Nepal

2018 ◽  
Vol 35 ◽  
pp. 9-22
Author(s):  
S. Sankhi ◽  
K.R. Sapkota ◽  
B. Regmi
Keyword(s):  
Semen Quality ◽  
Sperm Concentration ◽  
Quality Parameters ◽  
Bull Semen ◽  
Livestock Breeding ◽  
Abnormal Sperm ◽  
Breeding Center ◽  
Live Sperm ◽  
Level Of Significance ◽  
Effect Of Age

The present study was undertaken to determine the effect of age and frequency of collection on quality parameters of Jersey bull semen at National Livestock Breeding Center (NLBC), Pokhara, Kaski district. Nine Jersey bulls were selected randomly from NLBC. To obtain the effect of age, six bulls of three different age interval (3- 4, 5-7 and 8-9 years) were selected randomly. Remaining three bulls were used to determine the effect of frequency of collection on semen quality. Studies were conducted for three months (Nov/Dec to Dec/Jan 2017). Semen quality was analyzed using CASA system and SMILE software. ANOVA and paired t- tests were applied to find out level of significance. All the statistical analyses were done using SPSS 17.0. Sperm concentration (0.551 ±0.004 to 1.084 ± 0.014x109), post- thaw motility (42.44±0.11 to 51.72±0.03 %), live sperm (45.62 ± 0.13 to 51.23±0.15 %), and abnormal sperm (11.57±0.12 to 19.59±0.24%) were significantly affected by the age of the bulls. Semen quality was found superior in the bulls belonging to 5-7 years of age intervals. On the other hand, volume (3.98±0.09 to 6.72±0.06 ml), initial motility (72.68±0.02 to 78.64±0.15 %) and pre-filling motility (71.34±0.17 to 77.62±0.02 %) did not differ significantly among different age intervals. Significantly, higher value for semen volume (3.98±0.12 to 6.02±0.14 ml), sperm concentration (0.572±0.119 to 1.095±0.015) and post- thaw motility (40.34±0.21 to 48.54±0.06% was obtained on second collection. However, initial motility (70.58±0.06 to 75.64±0.13 %), prefilling motility (68.11±0.12 to 71.62±0.02 %), live sperm (53.27±0.54 to 56.17±0.02 %) and abnormal sperm (12.31±0.12 to 14.3±0.47 %) did not differ significantly between the frequencies of collection. Different quality parameters of semen like volume, concentration, initial motility, pre-filling motility, post thaw motility, live and normal spermatozoa percentage were higher in 5-7 years old bulls and in second collection of semen.

scholarly journals Raw Semen Characteristics of Three Different Indonesian Local Roosters

2016 ◽  
Vol 18 (3) ◽  
pp. 165
Author(s):  
Emilia Kamung Hambu ◽  
Raden Iis Arifiantini ◽  
Bambang Purwantara ◽  
Sri Darwati
Keyword(s):  
Sperm Motility ◽  
Semen Quality ◽  
Mass Movement ◽  
Sperm Concentration ◽  
Semen Volume ◽  
Sperm Abnormality ◽  
Microscopic Evaluation ◽  
Semen Characteristics ◽  
Live Sperm ◽  
Better Than

Indonesia has agreat variety of roosters, either indigenous type as well as exotic and cross breed. The purpose of this experiment was to study the characteristics of semen from three types of Indonesian local roosters such as Merawang, Kampung and crosses Sentul Kampung with Kedu (SK Kedu). A total of 15 roosters consist of  Merawang roosters,  Kampung, and SK Kedu roosters were 5 each. The semen was collected 3 times a week by dorso-abdominal and cloaca massage method. The parameters evaluation was macroscopic characteristics consist of volume, color, consistency, and pH. Microscopic evaluation of semen such as a mass movement, sperm motility, live sperm, sperm abnormality and sperm concentration. Results of this experiment showed that semen volume of  Merawang  (0.40±0.26 mL) was higher (p<0.05) compare to Kampung (0.24±0.12 mL) or  SK Kedu (0.16±0.10 mL) but no difference on semen color, consistency and semen pH. There were no difference in the mass movement, sperm motility and live sperm as well as on sperm abnormality among three types of roosters.  Sperm  concentration of Merawang (4490 million mL-1) was significantly higher than Kampung (3245 million mL-1) and the SK Kedu roosters (3751 million mL-1). Its was conclude that Merawang roosters had good semen quality better than Kampung and SK Kedu roosters

scholarly journals The effect of freezing on cryosurvival of yak sperm

2018 ◽  
Vol 15 (2) ◽  
pp. 215-218
Author(s):  
S Deori
Keyword(s):  
Liquid Nitrogen ◽  
Sperm Motility ◽  
Artificial Insemination ◽  
Sperm Concentration ◽  
Egg Yolk ◽  
Mean Values ◽  
Frozen Semen ◽  
Sperm Abnormality ◽  
Semen Characteristics ◽  
Live Sperm

A study was carried out to study the effect of freezing on cryosurvival of yak semen. Artificial insemination in yak is still in infancy. Semen cryopreservation and use of artificial insemination can be applied in yak husbandry for conservation and rapid multiplication of superior germplasm. Semen was collected from four adult yak bulls using artificial vagina method managed under uniform conditions. A total of 40 ejaculates comprising of 10 ejaculates each bull were collected following twice a week schedule and evaluated for fresh semen characteristics. The fresh yak semen characteristics viz. ejaculate volume (ml), mass activity (0-4), initial sperm motility (%), sperm concentration (x 106/ml), live sperm (%), sperm abnormality (%) and intact acrosome (%) were 3.10 ± 0.18, 3.53 ± 0.96, 83.89 ± 2.87, 1180.22 ± 42.32, 77.63 ± 4.23, 8.45 ± 3.33 and 93.61 ± 3.78 respectively. The ejaculates were diluted (1:10) with Tris extender consisting of 6.4 ml glycerol and 20 ml of fresh egg yolk. Straws were equilibrated at 5°C for 4 hours followed by exposure to liquid nitrogen vapour for 10 minutes and finally transferred to liquid nitrogen container for storage. The cryosurvival rate was studied after 7 days of storage in liquid nitrogen. The frozen semen was thawed in warm water (37°C) for 30 seconds for evaluation. Mean values of postthaw sperm motility (%), live sperm (%) and intact acrosome (%) in yaks were 55.67 ± 4.67, 65.62 ± 3.23 and 89.26 ± 3.67 respectively. In conclusion, yak semen has a better cryosurvival while freezing in tris extender with 6.4 per cent glycerol and 20 per cent egg yolk following an equilibration period of 4h.SAARC J. Agri., 15(2): 215-218 (2017)

Effect of water sprinkling during the hot-dry summer season on semen quality of Holstein bulls in Saudi Arabia

1992 ◽  
Vol 55 (1) ◽  
pp. 59-63
Author(s):  
M. S. Salah ◽  
F. D. El-Nouty ◽  
M. R. Al-Hajri
Keyword(s):  
Saudi Arabia ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Experimental Period ◽  
Summer Season ◽  
Cooling Period ◽  
Sperm Counts ◽  
Sperm Abnormality ◽  
Blood Haemoglobin ◽  
Holstein Bulls

AbstractTen Holstein bulls, 4 to 5 years old were used to study the effect of cooling the bulls on their semen quality. Five bulls were sprinkled with water for 15 min five times a day throughout a 6-week period of the summer season (hot-dry) in Saudi Arabia, and the other five bulls acted as the control and were not sprinkled with water. Semen quality was improved significantly during the cooling period. This improvement was evident from the significant increase in sperm motility (P < 0·01) and decrease in the percentage of both dead and morphologically abnormal (primary and secondary) spermatozoa per ejaculate of cooled bulls (P < 0·01). The effect of cooling was more marked through the last 3 weeks than during the first 3 weeks of the experimental period. Ejaculate volume, sperm concentration and hence total sperm counts did not differ significantly between the cooled and the non-cooled bulls. Concerning the type of abnormal spermatozoa, the overall secondary abnormality was higher than the primary in both the cooled and the control groups, with higher values for the control (P < 0·01). Only the mid piece showed higher primary defects than the secondary. The pyriform heads, the coiled mid pieces and tails and the bent tails were the main sources of variations (P < 0·01) in the incidence of sperm abnormality between the cooled and the non-cooled bulls. Cooling the bulls during the heat stress period also lowered their rectal temperature and increased blood haemoglobin level without affecting the packed cell volume

In vitro Fertilisation Capacity of Frozen Crossbred Bull Semen Cryopreserved During Different Seasons in Kerala

2021 ◽  
Author(s):  
M.M. Revathy ◽  
R.S. Abhilash ◽  
C. Jayakumar ◽  
P.K. Magnus ◽  
K. Raji ◽  
...  
Keyword(s):  
Rainy Season ◽  
Monsoon Season ◽  
Summer Season ◽  
Maximum Temperature ◽  
In Vitro Fertilisation ◽  
Post Monsoon ◽  
Bull Semen ◽  
Post Monsoon Season ◽  
Different Seasons

Background: Assessment of semen quality of bulls in frozen semen stations is of paramount importance as they are used for inseminating large number of cattle. The present study was conducted to assess the in vitro fertilisation capacity of crossbred bull semen cryopreserved during different seasons in Kerala as reports of such an evaluation are scarce.Methods: Semen samples from six crossbred bulls of same exotic inheritance, cryopreserved during rainy, post monsoon and summer seasons were procured from KLDB, Dhoni. The collected samples were evaluated for their in vitro fertilisation potential.Result: Hot dry summer season in Kerala adversely affects the fertilisation capacity of spermatozoa. Rainy season was observed to be the most favourable season for good quality semen production and post monsoon season was intermediate between summer and rainy season. It can be concluded from the present investigation that semen cryopreserved during summer season have lower fertilisation and cleavage rate than rainy and post monsoon season. This might be due to the harmful effect of significantly higher average maximum temperature and lower relative humidity occurred in the area during summer season on spermatogenesis.

Fresh and Post-Thaw Seminal Attributes of Jafarabadi Buffalo Bulls

2020 ◽  
Vol 15 (03) ◽  
pp. 45-48
Author(s):  
K. H. Parmar ◽  
FS Kavani ◽  
TK Patbandha ◽  
SS Parikh
Keyword(s):  
Semen Quality ◽  
Sperm Concentration ◽  
Conception Rate ◽  
Mean Values ◽  
Abnormal Sperm ◽  
The Mean ◽  
Live Sperm ◽  
One Year ◽  
Acrosomal Integrity

The study was conducted on four Jafarabadi breeding bulls, 5-6 years old to know the fresh and post-thawed seminal characteristics based on total of 192 semen ejaculates evaluated and cryopreserved over one year period. The mean values of fresh neat seminal characteristics of Jafarabadi bulls, viz., ejaculate volume (ml), colour/density (score), sperm concentration (million/ml), mass activity (score), initial motility (%), live sperm (%), abnormal sperm (%), HOS reactive sperm (%) and acrosomal integrity (%) were 5.19±0.18, 2.38±0.10, 1253.36±24.75, 3.73±0.05, 80.31±0.05, 86.20±0.64, 5.00±0.40, 85.75±0.43 and 93.56±0.56, respectively, whereas the mean post-thawed sperm characteristics, viz., progressive sperm motility, live sperm (%), abnormal sperm (%), HOS reactive sperm (%), acrosomal integrity (%) and first insemination conception rate (%) observed were 57.60±0.36, 66.34±0.53, 8.85±0.33, 56.97±0.46, 75.26±0.17 and 44.63±0.14, respectively. The semen quality of fresh and post-thawed samples observed was within normal limit for use in breeding program with satisfactory first insemination conception rate.

Seasonal changes in hematological and biochemical profile of dairy cows at high altitude cold desert

2016 ◽  
Vol 5 (12) ◽  
pp. 5156 ◽  
Author(s):  
Arup Giri ◽  
Vijay Kumar Bharti* ◽  
Sahil Kalia ◽  
Vineeth Ravindran T. ◽  
Puneet Ranjan ◽  
...  
Keyword(s):  
High Altitude ◽  
Milk Production ◽  
Hematological Parameters ◽  
Winter Season ◽  
Summer Season ◽  
Mean Corpuscular Hemoglobin ◽  
Cold Desert ◽  
Different Seasons ◽  
Humidity Index ◽  
Jersey Cows

Cattle are the main source of livelihood for small farmers in high altitude cold desert. They also provide draught power, social and cultural strength. Cattle, therefore, contribute to subsistence farming and enhance the sustainability of smallholder farming systems. Determination of nutritional and health status of cattle is important in modern animal agriculture. Haematological and biochemical aids have been used to identify status of cattle. Keeping this in view, the present study was undertaken to establish hematological and biochemical responses on milk production of Jersey cows at high altitude production in two different seasons (summer and winter). For each period, ambient temperature and relative humidity were recorded and the temperature-humidity index (THI) was calculated as indicator of thermal comfort for cattle. A total of 140 (70 cows in each season) clinically healthy lactating Jersey cows aged 2 – 17 years at 3327 – 3575-meter altitude from mean sea level in the Ladakh region of Jammu and Kashmir, India, were used to evaluate the effect of environmental conditions on haematological parameters. The hematological parameters were estimated by using an automatic blood analyzer and biochemical test performed by the serum semi-auto analyzer. Among the hematological parameters, white blood cell (WBC), lymphocyte (LYM), granulocytes (GRAN), red blood cells (RBC), hemoglobin (Hb), hematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), and platelets (PLT) were analyzed. The results showed a significant difference in most of the parameters due to the variation in ambient temperature, relative humidity and temperature-humidity index. A significant increase (p<0.05) in RBC, Hb, and HCT was recorded in winter season as compared to the summer season. MCV, MCH, MCHC and PLT levels also exhibited the similar trend whereas, the WBC, lymphocyte, monocytes, and granulocytes values found were higher in summer compared to the winter season (p<0.05). However, in case of biochemical profile, glucose and aspartate aminotransferase (AST) level showed a significant increase (p<0.05) in summer season but level of albumin went significantly higher (p<0.05) in the winter season. Milk production level was significantly higher (p<0.05) in the summer season. These results provide an insight into the hematological and biochemical responses of Jersey cows to different environmental conditions. As the hematological and biochemical profiles of dairy cows has been altered in response to the different season which ultimately affected on the milk production. This study will be helpful for the better dairy cattle management in different seasons for higher production at the cold arid high altitude region.

scholarly journals Seasons and pediatric dermatoses: a cross-sectional observational study in an urban skin hospital

2021 ◽  
Vol 7 (4) ◽  
pp. 513
Author(s):  
Laxmi B. Horatti ◽  
Kumar Dilip N. R. ◽  
A. R. Shashikiran
Keyword(s):  
Seasonal Variation ◽  
Observational Study ◽  
Skin Diseases ◽  
Winter Season ◽  
Climatic Conditions ◽  
Summer Season ◽  
Cross Sectional ◽  
The Status ◽  
Different Seasons ◽  
Skin Conditions

<p class="abstract"><strong>Background:</strong> It is a well-known that climatic conditions and seasonal variation have a direct effect on skin and the prevalence of certain skin diseases change directly with changing seasons. The presence of certain skin diseases in children reflect the status of health, hygiene and personal cleanliness of the society. The aim of the study was to know the prevalence of various skin conditions in different seasons.</p><p class="abstract"><strong>Methods:</strong> All fresh cases of children under 12 years of age attending the skin OPD were recorded and were divided based on the three seasons i.e.; summer winter and rainy. The results were statistically evaluated.</p><p class="abstract"><strong>Results:</strong> The top six conditions of our study were impetigo, miliaria, pityriasis alba, scabies, xerosis and papular urticaria. Impetigo and miliaria were more common during summer season, xerosis during winter season and scabies during rainy season.</p><p class="abstract"><strong>Conclusions:</strong> Different climatic conditions have varied effect on the skin and may lead to various dermatoses. To effectively corelate between seasons and skin conditions more such extensive studies on different population and ethnic groups have to be conducted.</p>

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