scholarly journals POTENCY OF Γ-ORYZANOL-RICH BLACK RICE BRAN (ORYZA SATIVA L. INDICA) EXTRACT FOR TYROSINASE INHIBITION

2020 ◽  
pp. 90-93
Author(s):  
AFIFAH VARDHANI ◽  
MAHDI JUFRI ◽  
ERNI PURWANINGSIH
Keyword(s):  
Oryza Sativa ◽  
Rice Bran ◽  
High Performance ◽  
Oryza Sativa L ◽  
Ethanolic Extract ◽  
Inhibition Activity ◽  
Tyrosinase Inhibition ◽  
Black Rice ◽  
Black Rice Bran ◽  
Skin Lightening

Objective: The objectives of this study were to quantify γ-oryzanol in an ethanolic extract of Oryza sativa L. Indica (black rice) bran and to evaluate its activity as a tyrosinase inhibitor. Methods: Black rice bran was extracted via maceration in 96% ethanol, and the γ-oryzanol concentration in the extract was measured through high-performance liquid chromatography. The applicability of the extract as a skin lightening agent was determined by evaluating its tyrosinase inhibition activity. Results: The dry rice bran contained 118.572 mg/g of γ-oryzanol, and the extract inhibited tyrosinase activity at an IC50 of 74.8%. Conclusion: The black rice bran extract was sufficiently potent for use in skin lightening formulations.

scholarly journals Evaluating the Efficacy of Lotion Containing Black Rice Bran (Oryza sativa L. indica) Extract as Skin Brightening Agent: A Clinical Trial

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Mahdi Jufri ◽  
Afifah Vardhani ◽  
Erni Purwaningsih
Keyword(s):  
Oryza Sativa ◽  
Rice Bran ◽  
Oryza Sativa L ◽  
Ethanolic Extract ◽  
P Value ◽  
Rice Grain ◽  
Black Rice ◽  
Rice Bran Extract ◽  
Black Rice Bran ◽  
Skin Lightening

Background: Ultraviolet exposure is an extrinsic factor to initiate melanogenesis, the process of melanin formation in the skin. Nowadays, natural ingredients tend to be more prevalent in cosmetic formulations due to consumers’ concern about synthetic ingredients and the risks they may represent for human health. Rice bran, the outer layer of a rice grain, can be utilized as a skin-lightening agent. Objectives: This study aimed to determine the efficacy of a lotion containing black rice bran (Oryza sativa L. indica) ethanolic extract as a skin lightening agent. Methods: The black rice bran ethanolic extract was formulated into oil in water (o/w) lotion. In this study, 34 women applied the lotion at one side of the forearm and base placebo lotion as control at the other side of forearm. The results were tested with a paired t-test by GraphPad Prism 8.3.0 software. Results: There was a significant decrease in the melanin index and erythema index in the forearm with a lotion containing black rice bran extract (P-value < 0.0001). Conclusions: The lotion containing 10% black rice bran extract was effective as a skin lightener because it effectively reduced skin melanin production when applied topically.

scholarly journals Gastroprotective Activities of Ethanol Extract of Black Rice Bran (Oryza sativa L.) in Rats

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3812
Author(s):  
Peerachit Tonchaiyaphum ◽  
Warangkana Arpornchayanon ◽  
Parirat Khonsung ◽  
Natthakarn Chiranthanut ◽  
Pornsiri Pitchakarn ◽  
...  
Keyword(s):  
Gastric Ulcer ◽  
Oryza Sativa ◽  
Rice Bran ◽  
Oryza Sativa L ◽  
Water Immersion ◽  
Rice Variety ◽  
Ethanol Extract ◽  
Gastric Ulcers ◽  
Black Rice ◽  
Black Rice Bran

Black rice is a type of rice in the Oryza sativa L. species. There are numerous reports regarding the pharmacological actions of black rice bran, but scientific evidence on its gastroprotection is limited. This study aimed to evaluate the gastroprotective activities of black rice bran ethanol extract (BRB) from the Thai black rice variety Hom Nil (O. sativa L. indica) as well as its mechanisms of action, acute oral toxicity in rats, and phytochemical screening. Rat models of gastric ulcers induced by acidified ethanol, indomethacin, and restraint water immersion stress were used. After pretreatment with 200, 400, and 800 mg/kg of BRB in test groups, BRB at 800 mg/kg significantly inhibited the formation of gastric ulcers in all gastric ulcer models, and this inhibition seemed to be dose dependent in an indomethacin-induced gastric ulcer model. BRB could not normalize the amount of gastric wall mucus, reduce gastric volume and total acidity, or increase gastric pH. Although BRB could not increase NO levels in gastric tissue, the tissue MDA levels could be normalized with DPPH radical scavenging activity. These results confirm the gastroprotective activities of BRB with a possible mechanism of action via antioxidant activity. The major phytochemical components of BRB comprise carotenoid derivatives with the presence of phenolic compounds. These components may be responsible for the gastroprotective activities of BRB. The 2000 mg/kg dose of oral BRB showed no acute toxicity in rats and confirmed, in part, the safe uses of BRB.

scholarly journals The Effect of Ethanolic Extract of Black and White Rice Bran (Oryza sativa L.) on Cancer Cells

2017 ◽  
Vol 21 (1) ◽  
pp. 63
Author(s):  
Rizal Maarif Rukmana ◽  
Nyoman Puniawati Soesilo ◽  
Rumiyati Rumiyati ◽  
Rarastoeti Pratiwi
Keyword(s):  
Cancer Cells ◽  
Rice Bran ◽  
Oryza Sativa L ◽  
Ethanolic Extract ◽  
Probit Analysis ◽  
Rice Cultivars ◽  
Chromatography Method ◽  
White Rice ◽  
Wide Range ◽  
Black Rice Bran

Indonesia has a wide range of rice cultivars and pigments. This rice can be used as a source ofphytochemical compounds for cancer prevention. This research aims to analyze the cytotoxic activitiesof the ethanolic extract of black rice bran of 4 local cultivars i.e. ‘Cempo Ireng’, ‘Woja Laka’, ‘Toraja’ and‘IR­64’ (white rice) on cancer cells and to determine the compounds groups of those extracts. First step,rice bran was extracted with ethanol. This extract was applied to Raji (a human Burkitt Lymphomacancer), HepG2 (a human liver cancer), and Vero (a nonhuman cell line) cells in order to measure thecytotoxic activities by using MTT assay. To determine descriptively the compounds groups of phenolics,flavonoids, terpenoids, steroids, and alkaloids the thin layer chromatography method was performed.The IC50 value was analyzed quantitatively by using probit analysis. Results showed that the IC50 valuesof ethanolic extract of rice bran ‘Woja Laka’, ‘Toraja’, ‘Cempo Ireng’ and ‘IR 64’ on HepG2 cells were857.23±99.19; 1,896.55±83,8; 1,494.47±87.81 and 727.89±145,97 µg/ml respectively. The IC50 on Raji cellswere 816.61±85.31; 1,079.93±28.31; 1,627.82; ±119.82, and 769.33±61.43 µg/ml respectively. The IC50 onVero cells were 1,295.2±37; 1,232.07±165.51; 1,874.14±169.56, and 724.4±122.79 µg/ml respectively. Theethanolic extracts of rice bran from four cultivars contain phenolics, flavonoids, terpenoids, and steroids.However, alkaloids could not be detected. The variety of rice cultivars indicates the variation of cytotoxicactivities on cancer cells. The ethanolic extracts of rice bran from those four rice cultivars contain similarkinds of organic compounds groups but vary in the Rf values.

scholarly journals Anthocyanin, nutrient contents, and antioxidant activity of black rice bran of Oryza sativa L. ‘Cempo Ireng’ from Sleman, Yogyakarta, Indonesia

2018 ◽  
Vol 22 (1) ◽  
pp. 49 ◽  
Author(s):  
Pratiwi Apridamayanti ◽  
Rarastoeti Pratiwi ◽  
Yekti Asih Purwestri ◽  
Woro Anindito Sri Tunjung ◽  
Rumiyati Rumiyati
Keyword(s):  
Antioxidant Activity ◽  
Rice Bran ◽  
Oryza Sativa L ◽  
Radical Scavenging ◽  
Anthocyanin Content ◽  
Rice Cultivars ◽  
Black Rice ◽  
Nutrient Contents ◽  
Macro And Micronutrients ◽  
Black Rice Bran

The chemical contents and health benefits of black rice bran of some rice cultivars have been investigated. However, there has been little research on the ‘Cempo Ireng’ cultivar from Sleman, Yogyakarta. The aim of this present study was to determine the anthocyanin, antioxidant activity, and macro- and micronutrients contents of black rice bran from this local cultivar. The anthocyanin in the black rice bran was extracted using the maceration method with methanol as a solvent. The extract obtained was separated through a preparative thin layer chromatography (TLC) of silica GF254 and a mobile phase composed of n-butanol, acetic acid, and water. Two fractions were collected and analyzed for the anthocyanin content. The preparative TLC spots were separated for further detection and measurement of cyanidin 3-O-glucoside using HPLC followed by LC-MS. The antioxidant activity of the fractions were measured using the DPPH free radical scavenging method. The results showed that the anthocyanin in fraction 1 was identified as cyanidin 3-O-glucoside (66.1 ± 10.6 µg/g). The IC50 of fractions 1 and 2 were 200.96 and 218.36 µg/mL, respectively. Analysis of the macro- and micronutrients revealed that the black rice bran of ‘Cempo Ireng’ had nutrient contents comparable with other rice cultivars. Therefore, this local black rice bran can be used as a source of antioxidants and macro-- and micronutrients.

scholarly journals EFEK KOPIGMENTASI DARI KATEKOL DAN TANIN TERHADAP STABILITAS ANTOSIANIN BEKATUL BERAS KETAN HITAM (Oryza sativa glutinosa) SELAMA PENYIMPANAN [Copigmentation Effect of Catechol and Tannin on Stability of Glutinous Black Rice Bran (Oryza sativa glutinosa) Anthocyanins During Storage]

2018 ◽  
Vol 23 (1) ◽  
pp. 31
Author(s):  
Dian Wulandari ◽  
Tirza Hanum ◽  
Azhari Rangga
Keyword(s):  
Oryza Sativa ◽  
Rice Bran ◽  
Storage Time ◽  
Life Time ◽  
Molar Ratio ◽  
Black Rice ◽  
Color Retention ◽  
Anthocyanin Concentration ◽  
Black Rice Bran ◽  
And Storage

Copigmentation has been suggested as an effective method to improve color and stability of anthocyanins and storage time.  Catechol and tannin can be used as a copigment to improve stabilize color of anthocyanins. This study aims to examine the effect of molar ratio of copigment to anthocyanin and storage time which stabilize anthocyanin.This study was arranged in factorial Randomized Complete Block Design (RCBD) with three replications. First factor were molar ratio 0:1, 50:1, 100:1; and second factor were time of storage 0, 10, 20, 30, 40 days at room temperature (28oC ± 2 oC).  Color and stability of anthocyanins were evaluated by thrugh determination of bathochromic and hyperchromic, anthocyanins concentration, retention, and kinetic parameters.  Variables were analyzed by Tuckey test, Bartlet test, and Polynomial Orthogonal test.The results showed that anthocyanin content of glutinous black rice bran extract was 426 mg/100g. Copigmentation with catechol resulted in bluish color (hypsochromic), while tannin resulted in reddish color (bathochromic).  The best molar ratio of catechol copigment was 50: 1 showed by decreasing of anthocyanin concentration of 0.35mM/day or 39.29mg/100g/day, color retention 12.78%/hour, and half-life time 8.66 hours. The best molar ratio of tannins copigment was 100: 1 showed by decreasing of anthocyanin concentration of 0.07mM/day or 7.86mg/100g/day, color retention was 2.39%/day, and half-life time of 19.80 hours.

scholarly journals The Role of Black Rice Bran (<i>Oryza sativa</i> L. “Sembada Hitam”) on Levels of Malondialdehyde in Induction Human Umbilical Vein Endothelial Cell Serum Preeclampsia

2020 ◽  
Vol 10 (12) ◽  
pp. 1686-1692
Author(s):  
David Randel Christanto ◽  
Johanes C. Mose ◽  
Tety Yuniarti ◽  
Muhammad Begawan Bestari ◽  
Yekti Asih Purwestri ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Endothelial Cell ◽  
Rice Bran ◽  
Umbilical Vein ◽  
Black Rice ◽  
Human Umbilical Vein ◽  
Black Rice Bran
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