scholarly journals Chemosystematic of Enterobacteriaceae Familia Obtained from Blood Cultures Based on Total Protein Profiles

2015 ◽  
Vol 18 (1) ◽  
pp. 58
Author(s):  
Sri Darmawati ◽  
Langkah Sembiring ◽  
Widya Asmara ◽  
Wayan T. Artama ◽  
Syaiful Anwar
Keyword(s):  
Key Words ◽  
Total Protein ◽  
Reference Strain ◽  
Protein Profile ◽  
Blood Cultures ◽  
Protein Profiles ◽  
E Coli ◽  
Relationship Analysis ◽  
Pair Group ◽  
Sds Page

The purpose of this study was to determine the chemosystematic of 14 strains of bacteria in blood cultures from Semarang using 1 reference strain S. typhi NCTC 786, based on the total protein profi les with the similarity relationship analysis based on Simple Matching Coeffi cient (SSM) analysis and algorithm methodof unweighted pair group with averages (UPGMA) presented in a dendrogram. The results showed that thechemosystematic based on the total protein profi les using SDS-PAGE method can classify the member ofbacterial strains of each species. The Clusters respectively consist of 4 strains of S. typhi (similarity: 89.7%),2 strains of Ser. marcescens (similarity: 89.7%), two strains of E. coli, and one strain of Salmonella ssp, S. typhi NCTC 786 (similarity: 100%). Those three incorporated clusters had the similarity value of 75.3%. Those four strains of Ent. cloacae composed in one cluster (similarity: 100%) are incorporated in a cluster consisting of one strain of Kleb. pneumoniae (similarity: 92.9%). Both clusters were incorporated in a cluster consisting of S. typhi NCTC 786 (similarity: 67.9%). Key words: Enterobacteriaceae, chemosystematic, blood cultures, protein profile

scholarly journals Effect Acupuncture for Low Back Pain; Biochemical and Protein Profile Analysis

2020 ◽  
Vol 16 (2) ◽  
pp. 207-215
Author(s):  
Suwaji Handaru Wardoyo ◽  
Solichan Badri
Keyword(s):  
Low Back Pain ◽  
Blood Serum ◽  
Total Protein ◽  
Profile Analysis ◽  
Protein Profile ◽  
Protein Band ◽  
Low Back ◽  
Protein Profiles ◽  
Sds Page ◽  
Blood Serum Protein

Low back pain (LBP) is one of the common symptoms experienced by some of the world community. Acupuncture as a method of healing a disease offers a solution for LBP symptom. This study aimed to determine the effect of acupuncture with local and Yamamoto New Scalp Acupuncture (YNSA) points with biochemical analysis related to total protein, albumin, and globulin, and analysis of blood serum protein profiles using the SDS-PAGE method. This research was conducted using sterile acupuncture needles that inserted in patients with several local points, namely BL22 (Sanjiaoshu), BL23 (Shenshu), BL24 (Qihaishu), BL25C (Dachangshu), and BL26C (Guanyuanshu) and combined with the YNSA points through somatotope D points and extra lumbar points H and I. Blood serum was collected for total protein, albumin, and globulin analysis. That blood serum was also used for protein profile analysis using Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) method. The results showed that six times of acupuncture treatments gave positive correlation in total protein, albumin, and globulin. Analysis of blood serum protein profiles using SDS-PAGE showed a protein band of about 12 KDa and it might be a interleukin-13 protein. The results were expected can be useful as scientific information especially related to acupuncture for LBP treatment and this of about 12 KDa protein band can be used as biomarker candidates for the symptoms.

scholarly journals Evaluation of Non-climacteric Senescence of Gladiolus sp. Flowers

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 760A-760
Author(s):  
G.H. Pemberton ◽  
Terril A. Nell ◽  
James E. Barrett
Keyword(s):  
Total Protein ◽  
Acc Synthase ◽  
Total Protein Content ◽  
Protein Profiles ◽  
Flower Longevity ◽  
Sds Page ◽  
Specific Proteins ◽  
Postharvest Life ◽  
Relationship Of ◽  
The Relationship

Senescence of gladiolus flowers, like many geophytes, does not involve a climacteric burst of ethylene. Eleven gladiolus cultivars were screened and all were non-climacteric (NC) for both respiration and ethylene production. Average ethylene levels for individual flowers were 0.5 μl C2H4/kg per h or less. As in other NC flowers, protein synthesis may be linked to senescence. Our goal was to identify specific proteins that were involved in the senescence process that could be used as indicators of postharvest longevity. SDS-PAGE protein profiles of cut gladiolus flowers were determined from a tight bud stage to senescence. Both increases and decreases were observed in major polypeptides that may be connected to postharvest flower longevity. Total protein content of gladiolus flower petals decreased by ≈70% during the profile period. This could explain the relatively short postharvest life of 3 to 5 days for individual gladiolus flowers. Total protein profiles were probed with an ACC synthase antibody to establish the relationship of this enzyme in NC senescence.

scholarly journals The Quality of Dory Fillets based on Water Soluble Protein, Color, and Myoglobin Concentration

2016 ◽  
Vol 19 (1) ◽  
pp. 44
Author(s):  
Nurfajrin Nisa ◽  
Mala Nurilmala ◽  
Tati Nurhayati ◽  
Nurlisa Butet
Keyword(s):  
Soluble Protein ◽  
Protein Profile ◽  
Water Soluble ◽  
Color Measurement ◽  
Protein Profiles ◽  
Water Soluble Protein ◽  
Sds Page

<p>Fillet of dory is very easy to be find in Indonesian market with various brand and produsen.<br />Imported dory fillet is preferred by consumer so far because it has a white color compare than local<br />fillets. Color is the important parameter that used by consumers to determine the quality of filet. This<br />study was aimed to determine the quality of local and imported fillets, including protein profile using<br />SDS PAGE, color measurement, and myoglobin extractability. The results of water soluble protein profiles<br />showed dory fillet contained 13-15 bands. The redness value (a*) of local fillet (DN, DL, DM) was higher<br />compared others. However, imported fillet (DI) had the highest if redness index (a/b). Imported fillet (DI)<br />showed the lowest concentration of myoglobin compared other samples.</p>

scholarly journals A new method of protein extraction from red wines

OENO One ◽  
2013 ◽  
Vol 47 (3) ◽  
pp. 213
Author(s):  
Anne-Claire Silvestri ◽  
Jessica Sabatier ◽  
Julien Ducruet
Keyword(s):  
Protein Extraction ◽  
Protein Profile ◽  
Protein Profiles ◽  
Red Wines ◽  
Sds Page ◽  
Fining Agents ◽  
The Stability ◽  
Main Components ◽  
Separation Of Proteins ◽  
Grape Varieties

<p style="text-align: justify;"><strong>Aim</strong>: Wine is a very complex medium and is often evaluated according to its main components like alcohol, sugar, tannins, and acid levels. Proteins are rarely considered in this evaluation because their concentrations are only a few mg/L of wine. However, in an enological context, proteins appear to be more and more important, in particular for the stability of wines with protein haze problems. The study of proteins is less obvious in red wines than in white wines because the proteins are strongly tied to tannins, which makes their extraction and analysis even more difficult.</p><p style="text-align: justify;"><strong>Methods and results</strong>: This article describes a technique for the separation of proteins from tannins thanks to a methanol/chloroform emulsion in an acid solution. The protein extract, obtained after 4 hours, was later analyzed by SDS-Page and the protein profile of the wine established. Experiments showed that the protein profiles remained the same during the different stages of the winemaking process, whereas the overall amount of proteins decreased. Characteristic protein profiles of different grape varieties were established, and it was also possible to visualize the presence of exogenous proteins from fining agents like albumin and casein.</p><p style="text-align: justify;"><strong>Conclusion</strong>: This procedure allowed the extraction of proteins from 8 red wine samples within 4 hours. It also made it possible to analyze the extracted proteins by SDS-Page without tannin interference within 2 hours.</p><p style="text-align: justify;"><strong>Significance and impact of the study</strong>: This method shows in a very promising manner how proteins might be extracted from red wines after being separated from their tannins. The extracted proteins are then available for analysis using even more advanced techniques such as ESI-QTof or ELISA.</p>

scholarly journals The Effect of Roasting on the Protein Profile and Antiradical Capacity of Flaxseed Meal

Foods ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1383
Author(s):  
Katarzyna Waszkowiak ◽  
Beata Mikołajczak
Keyword(s):  
Heat Treatment ◽  
Protein Profile ◽  
Protein Fractions ◽  
Maillard Reaction Products ◽  
Protein Profiles ◽  
Reaction Products ◽  
Flaxseed Meal ◽  
Sds Page ◽  
Roasting Temperature ◽  
Pre Treatment

Roasting is more and more often used as a pre-treatment of flaxseeds. However, the process can influence flaxseed proteins that may be crucial for their properties. The aim of this research was to study changes in the electrophoretic protein profile (SDS-PAGE) and the antiradical capacity of flaxseed meals after roasting. The roasting temperature (160, 180, and 200 °C) and flaxseed cultivars (golden and brown seed) were factors including in the study. The free (F-MRP) and bound-to-protein (B-MRP) Maillard reaction products were also analyzed. The most significant changes in the SDS-PAGE protein profiles of roasted seeds of each of the tested flax cultivars were observed for the 13 kDa protein fraction (decrease) and for the 19 kDa and 17 kDa fractions (increase). The research revealed a significant correlation between the roasting temperature and B-MRP content, and changes in the percentage share of those three protein fractions. The antiradical capacity of roasted flaxseeds decreased, as compared with untreated seeds. After roasting at 200 °C the antiradical capacity of flaxseeds improved slightly, probably due to the MRP formation, but it was still significantly lower than that of the raw seeds. The research provides novel information about key protein fractions that seem to be important changing during heat treatment.

scholarly journals Profil Protein Trypanosoma evansi dari Daerah Geografis Berbeda di Indonesia Tahun 2012-2014 dengan Sodium Dodecil Sulphate Polyacrylamide Gel Electrophoresis (TRYPANOSOMA EVANSI PROTEIN PROFILE OF DIFFERENT GEOGRAPHICAL AREAS ORIGIN IN INDONESIA

2018 ◽  
Vol 18 (4) ◽  
pp. 526
Author(s):  
Fitrine Ekawasti ◽  
Ichwan Yuniarto ◽  
Sulinawati Sulinawati ◽  
Didik Tulus Subekti
Keyword(s):  
Soluble Protein ◽  
Serological Test ◽  
Protein Profile ◽  
Polyacrylamide Gel Electrophoresis ◽  
Trypanosoma Evansi ◽  
Blue Staining ◽  
Protein Profiles ◽  
Coomassie Blue Staining ◽  
Coomassie Blue ◽  
Sds Page

Surra outbreak in 2012 has led to more than 1,700 animals have died in the province of East Nusa Tenggara (NTT) Indonesia. Surra case sporadically continues throughout the year in various areas, especially Kalimantan, Banten as well as other areas. Some reports reveal differences in protein profiles among multiple isolates of T. evansi. Therefore the purpose of this research were to find out the protein profile of each isolate T. evansi in Indonesia and the possible biological differences among them. Eleven isolates originating from the province of East Nusa Tenggara, South Kalimantan and Central Kalimantan, Banten, Lampung and Bengkulu has been isolated and purified Using DEAE. Trypanosoma isolate were frezeethawing repeatedly to obtain soluble protein. Furthermore, soluble protein is treated with heating or without heating and then each was run on SDS PAGE with Coomassie Blue staining. The protein profiles of all isolates were compared each other. The results showed that eleven isolates of T. evansi in Indonesia has a very diverse protein profile. Then for the purposes of development of diagnostic kit can be used whole lysate cell (WCL) as stock antigen in serological test process.

scholarly journals Effects of Different Methods of Slaughtering on Protein Expression in Chicken Meat

2012 ◽  
Vol 13 (1) ◽  
Author(s):  
Rahela Zaman ◽  
Hamzah Mohd. Nassir ◽  
Nafiu Bidemi Abdurrazq ◽  
Hamzah Mohd. Salleh ◽  
Mohammad Tariqur Rahman
Keyword(s):  
Skeletal Muscle ◽  
Spinal Cord ◽  
Protein Expression ◽  
Total Protein ◽  
Protein Profile ◽  
The Body ◽  
2D Page ◽  
Sds Page ◽  
Sharp Knife ◽  
Chicken Skeletal Muscle

ABSTRACT: This study investigated the variation in total protein profile in chicken skeletal muscle which is thought to be influenced by variations in the method of slaughtering. Two types of samples, depending on the method of slaughtering using a sharp knife, were examined. These were: (i) CT- neck was cut off completely and body was tied until the animal expired, and (ii) PR- neck was cut off partially leaving the spinal cord intact and the body was released immediately after slaughtering. Proteins, expressed in higher amounts, were mostly found to be distributed in the range of Mw 45-66 kDa -- as resolved using SDS-PAGE. 2D-PAGE resolution showed differences in protein expression between the samples. Protein spotted on the gel near pH 5.0 and Mw 116 kDa was found in the skeletal muscle from CT samples but were absent in the PR samples.ABSTRAK: Variasi profil jumlah protein di dalam otot skeletal telah dikaji dengan jangkaan bahawa ianya dipengaruhi oleh kaedah penyembelihan  yang berbeza. Berdasarkan kaedah penyembelihan, dua jenis sampel telah diperiksa: (i) CT – leher telah dipotong sepenuhnya dan badan haiwan diikat hingga mati and (ii) PR – leher dipotong separuh meninggalkan kord tulang belakang dalam keadaan sempurna dan badan dilepaskan serta-merta selepas penyembelihan. Kandungan protein didapati tinggi dalam taburan Mw 45-66 kDa sebagai terurai menggunakan SDS-PAGE. Sementara itu, kandungan protein yang berbeza diperhatikan di dalam penguraian 2D-PAGE. Tompok protein kelihatan pada gel sekitar pH 5 dan Mw 116 kDa telah dijumpai pada CT otot skeletal, tetapi tidak kelihatan pada sampel PR.KEYWORDS: 2D-PAGE; slaughtering; protein; slaughtering

scholarly journals Perbandingan Metode Hierarchical Cluster Analysis untuk Analisis Keragaman Hayati Trypanosoma evansi dari Indonesia Berdasarkan Profil Protein (COMPARISON OF HIERARCHICAL CLUSTER ANALYSIS METHODS FOR BIODIVERSITY ANALYSIS OF TRYPANOSOMA EVANSI

2018 ◽  
Vol 18 (4) ◽  
pp. 516
Author(s):  
Didik Tulus Subekti ◽  
Ichwan Yuniarto ◽  
Sulinawati Sulinawati
Keyword(s):  
Cluster Analysis ◽  
Hierarchical Cluster Analysis ◽  
Binary Data ◽  
Protein Profile ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Hierarchical Cluster ◽  
Trypanosoma Evansi ◽  
Protein Profiles ◽  
Sds Page

Hierarchical Clustering Analysis (HCA) has long been known to be useful for the analysis of biodiversity of microorganisms based on SDSPAGE protein profile (sodium dodecyl sulfate polyacrylamide gel electrophoresis). However, varying methods of HCA consequently produce variability of analysis results and interpretations. Therefore, it is necessary to evaluate and further determine the most appropriate method which could described the biodiversity based on protein profiles of T.evansi isolates from Indonesia. Eleven isolates of T.evansi from different geographic locations were run on SDS PAGE. Furthermore, SDS PAGE protein profiles from eleven isolates were converted into binary data and analyzed using five different methods of HCA i.e. Average Linkage, Complete Linkage, Single Linkage, Ward Linkage and McQuitty Linkage, respectively.Data were also analyzed by multidimensional scaling (MDS) and densitogram. The analysis showed that the dendrogram constructed with Ward Linkage gives the best results and corresponding with densitogram, MDS and able to describe the geographical origins of isolates.

Pomphorhynchus laevis (Acanthocephala): elevation of haemolymph protein concentrations in the intermediate host, Gammarus pulex (Crustacea: Amphipoda)

Parasitology ◽  
1993 ◽  
Vol 107 (2) ◽  
pp. 193-198 ◽  
Author(s):  
C. R. Bentley ◽  
H. Hurd
Keyword(s):  
Total Protein ◽  
Volume Ratio ◽  
Host Tissue ◽  
Gammarus Pulex ◽  
Protein Profiles ◽  
Pomphorhynchus Laevis ◽  
Tissue Weight ◽  
Sds Page ◽  
Respiratory Protein ◽  
One Way Anova

SUMMARYComparisons were made between the protein concentrations and protein profiles of uninfected Gammarus pulex and those infected with cystacanths of Pomphorhynchus laevis. Comparisons of the extractable haemolymph volumes from uninfected and infected G. pulex, when related to host tissue weight, showed that there was no difference in the host tissue/haemolymph volume ratio between infected and uninfected individuals. SDS-PAGE of haemolymph samples resolved 36 bands of protein, the major components being band 6(19 kDa) and 21(33 kDa). Densitometric analysis of haemolymph samples showed that infected gammarids had total protein concentrations elevated by 84%. The (88 kDa) subunit was identified as the monomer of the respiratory protein haemocyanin. This band was significantly elevated in infected individuals (one-way ANOVA P < 0.001); increasing from 30 to 45% of the total haemolymph proteins.

scholarly journals KERAGAMAN BEBERAPA TUMBUHAN CIPLUKAN (Physalis spp.) DI LERENG GUNUNG KELUD, JAWA TIMUR

2018 ◽  
Vol 17 (2) ◽  
Author(s):  
Nugraheni Hadiyanti ◽  
Supriyadi Supriyadi ◽  
Pardono Pardono
Keyword(s):  
Sea Level ◽  
Total Protein ◽  
Leaf Shape ◽  
Protein Profile ◽  
Morphological Diversity ◽  
Morphological Characters ◽  
Wild Plant ◽  
Flavonoid Compound ◽  
Leaf Margin ◽  
Protein Profiles

Ciplukan (Physalis spp.) is a wild plant which is that potentially used as a medicinal plant. Information related to the diversity of Physalis spp. in Indonesia is limited. The objectives of the research was to identify the diversity of wild Physalis sp. on Mt. Kelud, East Java based on morphological characters, total protein profiles, and flavonoid compounds. The research was conducted by survey on some gradients viz. 200–400, 400–600, 600–800, 800–1,000 and >1,000 m above sea level. Identification of morphological characters was focused on root, stem, leaf, flower and fruit covering 16 characters for scoring. Identification of total protein profile was performed by SDS-PAGE method and the flavonoid compound was analyzed using UV spectrophotometric method based on the colorimetric principle. The degree of diversity of Physalis spp. was determined based on morphological similarity and total protein profiles through cluster analysis. The results showed that Physalis spp. on Kelud was found up to 1,000 m above sea level. Altitude affected to the morphological diversity, total protein profile and the flavonoid compound. Morphological features that play a role in the diversity of Physalis sp. were the direction of growing stems, leaf shape, leaf margin, leaf surface, leaf stalk color, stain color on the inner neck of thr flower, and fruiting calyx color. Expression of total protein P. minima. shows similarity to P. angulata L. Physalis sp. in the low gradient have a higher flavonoid compound as compared to the middle and high gradients. There were two species of Physalis found on the gradient of Mt. Kelud, East Java, namely Physalis minima L. and Physalis angulata L.

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